Resource data sheet
DNA Bank Top
Please review the HTS results indicated by check icon below as well as clone information before placing your order.

pMXs-IP-GFP-Atg14

Retroviral vector for stable expression of human Atg14 with N-terminal EGFP.

Catalog number RDB19790
Resource name pMXs-IP-GFP-Atg14
Clone info. Retroviral vector for stable expression of human Atg14 with N-terminal EGFP.
Vector backbone pMXs-IP (plasmid)
Selectable markers Ampicillin (E. coli), Puromycin (mammalian cell)
Gene/insert name human WIPI1 cDNA; Aequorea victoria GFP (EGFP).
Depositor|Developer Mizushima, Noboru |
 
Sequence (full) RDB19790hts01.seqcheck assembled from experimentally sequenced data
Test sheet RDB19790_B2Jlp1-1.pdf 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Itakura, E. et al. Mol. Biol. Cell. 19(12): 5360-5372, 2008)
Additional terms and conditions:
Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Ordering
Order form [Credit Card Payment]  [Bank Transfer Payment]
MTA, for use for not-for-profit academic purpose [Word]
Please visit Information of Request for Distribution.[link] 
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Itakura, E. et al. Mol. Biol. Cell. 19(12): 5360-5372, 2008)。
付加的提供条件:
pMXsシリーズベクターについて:利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所 北村俊雄博士への謝辞の表明を必要とする。
提供依頼
提供依頼書 [Word]
提供同意書 (MTA, 非営利学術目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19790 pMXs-IP-GFP-Atg14 DNA solution


How to cite this biological resource

Materials & Methods section:

The pMXs-IP-GFP-Atg14 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19790).

Reference section:

Itakura, E., Kishi, C., Inoue, K., Mizushima, N., Beclin 1 forms two distinct phosphatidylinositol 3-kinase complexes with mammalian Atg14 and UVRAG. Mol. Biol. Cell 19 (12): 5360-5372 (2008). PMID 18843052. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles

original Itakura, E., Beclin 1 forms two distinct phosphatidylinositol 3-kinase complexes with mammalian Atg14 and UVRAG. Mol. Biol. Cell 19 (12): 5360-5372 (2008). PMID 18843052. [link to RRC of NBRP]