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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMRX-IP-GFP-LC3-RFP

Fluorescent probe to evaluate autophagic flux by the rat LC3 fused with GFP and RFP.

Catalog number RDB14601
Resource name pMRX-IP-GFP-LC3-RFP
Clone info. Fluorescent probe to evaluate autophagic flux. Expression vector of rat microtubule-associated protein 1 light chain 3 beta (LC3) fused with GFP and RFP. This probe is cleaved by endogenous ATG4 proteases into equimolar amounts of GFP-LC3 and RFP. The GFP-LC3 is degraded by autophagy, while RFP remains in the cytosol as an internal control. Thus, autophagic flux can be estimated by calculating the GFP/RFP signal ratio. Please refer DISCUSSION section of Kaizuka, T., Mol. Cell 64, 835-849, 2016 for the difference between pMRX-IP-GFP-LC3-RFP-LC3deltaG and this clone.
Comment Expression was confirmed by the depositor with immunoblotting and fluorescent microscopy. Please refer Saitoh, T. et al., FEBS Lett., 532 (1-2): 45-51, (2002) for the construction of pMRX-IP vector.
Vector backbone pMRX-IP (Plasmid)
Selectable markers Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name Rat LC3 cDNA Aequorea victoria GFP cDNA Discosoma sp. RFP cDNA
Depositor|Developer Mizushima, Noboru |
Other clones in our bank

External Database
Rat LC3 Aequorea victoria GFP
    Discosoma sp. RFP

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution (1) BIOLOGICAL RESOURCE is limited to for academic research in non-profit organization. (2) The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. (3) Any use of the BIOLOGICAL RESOURCE for profit purposes by a non-profit organization or any use of the BIOLOGICAL RESOURCE by a profit organization requires a separate license from the DEPOSITOR prior to distribution. (4) In publishing research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the original publication designated by the DEPOSITOR (Kaizuka, T. et al. Mol. Cell, 64 (4): 835-849, 2016) and an acknowledgment to the DEPOSITOR are requested. (5) The RECIPIENT must contact the DEPOSITOR in case of application for any patents or commercial use with the results from the use of BIOLOGICAL RESOURCE.
          Additional terms and conditions:
          Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          Approval form by depositor [Word]
          Remarks ((Additional form)) Approval Form(FormD)
          Remember that you will be working with samples containing infectious virus.
          This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
          Please obtain written approval of the depositor.
          提供案内 (日本国内) [open/close]

          提供条件 1. 非営利団体による学術研究に限る。2. 利用者は事前に寄託者の提供承諾書を得る。3. 当該リソースの非営利団体の営利目的での利用および営利団体の利用については、事前に寄託者との間で、別途使用許諾に係る契約の締結が必要である。4. 研究成果の公表にあたって寄託者の指定する文献(Kaizuka, T. et al. Mol. Cell, 64 (4): 835-849, 2016)を引用し、謝辞の表明を必要とする。5. 利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
          付加的提供条件:
          pMRXベクターについて:利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。
          寄託者による提供承諾書 [Word]
          備考 【追加提供依頼書】承諾書(FormD)
          このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
          Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。
          寄託者の書面による提供承諾が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB14601 pMRX-IP-GFP-LC3-RFP DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Morishita, H., A new probe to measure autophagic flux in vitro and in vivo. Autophagy 13 (4): 757-758 (2017). PMID 28121224.
          original Kaizuka, T., An Autophagic Flux Probe that Releases an Internal Control. Mol Cell. 2016 Nov 17;64(4):835-849 (2016). PMID 27818143.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Autophagy (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB19179_B1Ftp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: MMLV-pol_F
          Region: pol region,insert 5'(EGFP 5')
          Sequence file: RDB19179_B1Fta.seq check
          >14601_19179_B1Ft_2_MMLV-pol_F_150_E02_14_ABI24.ab1
              1 NNNNNNNNNN NNGGNATCGN AGCTTGGNNA CNCGCCGCCC ACGTGAAGGC TGCCGACCCC
             61 GGGGGTGGAC CATCCTCTAG ACTGCCGGAT CTCCCGCCGC CACCATGGTG AGCAAGGGCG
            121 AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC
            181 ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA
            241 AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCCTGA
            301 CCTACGGCGT GCAGTGCTTC AGCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA
            361 AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG GACGACGGCA
            421 ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC CGCATCGAGC
            481 TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG GAGTACAACT
            541 ACAACAGCCA CAACGTCTAT ATCATGGCCG ACAAGCAGAA GAACGGCATC AAGGTGAACT
            601 TCAAGATCCG CCACAACATC GAGGACGGCA GCGTGCAGCT CGCCGANCAC TACCAGCAGA
            661 ACACCCCNAT CNGCGANGGC CCNNNGCTGC TGCCNGANNN CCNCTNNNNN ANCNNNNNNN
          721 NNCNNNNNNN ANNNNCCNNN NNNNNNN
          //
          Primer: mRFP1-inner1
          Region: insert 3'(mRFP1 3'),IRES2
          Sequence file: RDB19179_B1Ftb.seq check
          >14601_19179_B1Ft_2_mRFP1-inner1_C03_09_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNTCCTCC CNGNNNGNNG GCNNNTTCAT CTACAAGGTG
             61 AAGCTGCGCG GCACCAACTT CCCCTCCGAC GGCCCCGTAA TGCAGAAGAA GACCATGGGC
            121 TGGGAGGCCT CCACCGAGCG GATGTACCCC GAGGACGGCG CCCTGAAGGG CGAGATCAAG
            181 ATGAGGCTGA AGCTGAAGGA CGGCGGCCAC TACGACGCCG AGGTCAAGAC CACCTACATG
            241 GCCAAGAAGC CCGTGCAGCT GCCCGGCGCC TACAAGACCG ACATCAAGCT GGACATCACC
            301 TCCCACAACG AGGACTACAC CATCGTGGAA CAGTACGAGC GCGCCGAGGG CCGCCACTCC
            361 ACCGGCGCCT AAAGATCCCA GTGTGGTGGT ACGGGAATTC CTGCAGGCCT CGAGGGCCGG
            421 CGCGCCGCGG CCGCTACGTA AATTCCGCCC CCCCCCCCCC TCTCCCNNNN NNNNNCCTAA
            481 CGTTNNNNNN CGAANCCNCT TGNANNANNN NNGGTGTGCG TTTGTCTNNN NNNTATTTNN
          541 NNNNNNNN
          //
          Primer: mVenus-M_F
          Region: insert mid(EGFP,rat LC3)
          Sequence file: RDB19179_B1Ftc.seq check
          >14601_19179_B1Ft_2_mVenus-M_F_B03_06_ABI24.ab1
              1 NNNNNNNNNN NNNGGNNTNN GGNNNANTTN ANGNTCCGCC ACAACATCGA GGNNGGCAGC
             61 GTGCAGCTCG CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG
            121 CCCGACAACC ACTACCTGAG CACCCAGTCC GCCCTGAGCA AAGACCCCAA CGAGAAGCGC
            181 GATCACATGG TCCTGCTGGA GTTCGTGACC GCCGCCGGGA TCACTCTCGG CATGGACGAG
            241 CTGTACAAGG GTGGCGGTGG CTCGGGCGGT GGTGGGTCGG GTGGCGGCGG ATCTATCGAT
            301 GAATTGAATT CCATGCCGTC CGAGAAGACC TTCAAACAGC GCCGGAGCTT CGAACAAAGA
            361 GTGGAAGATG TCCGGCTCAT CCGGGAGCAG CACCCCACCA AGATCCCAGT GATTATAGAG
            421 CGATACAAGG GTGAGAAGCA GCTGCCCGTC CTGGACAAGA CCAAGTTCCT TGTACCTGAT
            481 CACGTGAATA TGAGCGAACT CATCAAGATA ATTAGAAGGC GCCTGCAGCT CAATGCTAAC
            541 CAAGCCTTCT TCCTCCTGGT GAATGGGCAC AGCATGGTGA GTGTGTCCNC ACCCATCTCT
            601 GAAGTGTACG AGAGCGAGAG AGANGAAGAC GGCTTCCTGT ACNTGGNCNN NGNCNTCCNN
            661 GGAGNNGTTC GGNANNNNCN TCCTCCGANG NNNGTCNTNN NNNNNNTNNN NGNNNNNNNN
          721 NN
          //
          Primer: mCherry-check_R
          Region: insert mid(mRFP1,rat LC3)
          Sequence file: RDB19179_B1Ftd.seq check
          >14601_19179_B1Ft_2_mCharry-check_R_E03_15_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNTCC TCNNNNTTCN TCACGCGCTC CCACTTGAAG
             61 CCCTCGGGGA AGGACAGCTT CAAGTAGTCG GGGNTGTCGG CGGGGTGCTT CACGTAGGCC
            121 TTGGAGCCGT ACTGGAACTG AGGGGACAGG ATGTCCCAGG CGAAGGGCAG GGGGCCGCCC
            181 TTGGTCACCT TCAGCTTGGC GGTCTGGGTG CCCTCGTAGG GGCGGCCCTC GCCCTCGCCC
            241 TCGATCTCGA ACTCGTGGCC GTTCACGGAG CCCTCCATGC GCACCTTGAA GCGCATGAAC
            301 TCCTTGATGA CGTCCTCGGA GGAGGCCATC CCGAACGTCT CCTGGGAGGC ATAGACCATG
            361 TACAGGAAGC CGTCTTCATC TCTCTCGCTC TCGTACACTT CAGAGATGGG TGTGGACACA
            421 CTCACCATGC TGTGCCCATT CACCAGGAGG AAGAAGGCTT GGTTAGCATT GAGCTGCAGG
            481 CGCCTTCTAA TTATCTTGAT GAGTTCGCTC ATATTCACGT GATCAGGTAC AAGGAACTTG
            541 GTCTTGTCCA GGACGGGCAG CTGCTTCTCA NCCTTGTATC GCTCTATAAT CACTGGGATC
            601 TTGGTGGGGT GCTGCTCCCG GNTGAGCCNG GACATCTTCC NCTCTTTGNT CGAANCTCNN
          661 GCGCTGTTTG ANNGNCTTCT CNNACNNNNN NNNNN
          //
          Primer: gag-N_R
          Region: MMLV psi, R-U5
          Sequence file: RDB19179_B1Fte.seq check
          >14601_19179_B1Ft_2_gag-N_R_C02_08_ABI24.ab1
              1 NNNNNNNNNN NNCNGGCCNG GGCCNNTATT TTNNGACAAA TACAGAAACA CAGTCAGACA
             61 GAGACAACAC AGAACGATGC TGCAGCAGAC AAGACGCGCG GCGCGGCTTC GGTCCCAAAC
            121 CGAAAGCAAA AATTCAGACG GAGGCGGGAA CTGTTTTAGG TTCTCGTCTC CTACCAGAAC
            181 CACATATCCC TCCTCTAAGG GGGGTGCACC AAAGAGTCCA AAACGATCGG GATTTTTGGA
            241 CTCAGGTCGG GCCACAAAAA CGGCCCCCGA AGTCCCTGGG ACGTCTCCCA GGGTTGCGGC
            301 CGGGTGTTCC GAACTCGTCA GTTCCACCAC GGGTCCGCCA GATACAGAGC TAGTTAGCTA
            361 ACTAGTACCG ACGCAGGCGC ATAAAATCAG TCATAGACAC TAGACAATCG GACAGACACA
            421 GATAAGTTGC TGGCCAGCTT ACCTCCCGGT GGTGGGTCGG TGGTCCCTGG GCAGGGGTCT
            481 CCCGATCCCG GACGAGCCCC CAAATGAAAG ACCCCCGCTG ACGGGTAGTC AATCACTCAG
            541 AGGAGACCCT CCCNAGGAAC AGCGAGACCA CAAGTCGGAT GCAACTGCAA GAGGGTTTAT
            601 TGGATACACG GGTANCCGGG CGANTCNGTC AATCGNANGA NTGGCGCGCC NANTGAGGGG
            661 NTGTGGGCTC NTTTATTGAN NTCGGGGANC ANAANCGCGC GANNNGAANC NANNNCGAAN
          721 NGANNNNNNN NNN
          //
          Primer: pMFG_1
          Region: pol region,gag
          Sequence file: RDB19179_B1Ftf.seq check
          >14601_19179_B1Ft_2_pMFG_1_D03_12_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNTCTNGT NNTGTCAGGG TCAGGGNAGT TTACAAGGGG
             61 CGGGGGTGCC CCATATAAGA TCTCATATGG CCATATGGGG GCGCCTAGAG AAGGAGTGAG
            121 GGCTGGATAA AGGGAGGATC GAGGCGGGGT CGAACGAGGA GGTTCAAGGG GGAGAGACGG
            181 GGCGGATGGA GGAAGAGGAG GCGGAGGCTT AGGGTGTACA AAGGGCTTGA CCCAGGGAGG
            241 GGGGTCAAAA GCCAAGGCTT CCCAGGTCAC GATGTAGGGG ACCTGGTCTG GGTGTCCATG
            301 CGGGCCAGGT GAAAAGACCT TGATCTTAAC CTGGGTGATG AGGTCTCGGT TAAAGGTGCC
            361 GTCTCGCGGC CATCCGACGT TAAAGGTTGG CCATTCTGCA GAGCAGAAGG TAACCCAACG
            421 TCTCTTCTTG ACATCTACCG ACTGGTTGTG AGCGATCCGC TCGACATCTT TCCAGTGACC
            481 TAAGGTCAAA CTTAAGGGAG TGGTAACAGT CTGGCCCGGG CCCATATTTT CAGACAAATA
            541 CAGAAACACA GTCAGACAGA GACNACACAG AACGATGCTG CAGCAGACAA GACGCGCGGC
            601 GCGGCTTCNG TCCCAAACCG AAAGCAAAAA TTCNGANGGA GGCGGGAACT GNTTTAGNNT
            661 CTCGTCTCCT ACCAGANNCA CATATCCCTN CNTCTNANNG GGGGNGNANN NNNNNANTNN
          721 NNAANNNNTN NNNNNNNTTN TNNNNNNNNN N
          //
          Primer: mCherry-C_R
          Region: insert mid(mRFP1)
          Sequence file: RDB19179_B1Ftg.seq check
          >14601_19179_B1Ft_2_mCherry-C_R_C03_09_ABI24.ab1
              1 NNNNNNNNNN NNNCNNNNNG TCGGTCTTGT AGGCGCCGGG CAGCTGCACG GGCTTCTTGG
             61 CCATGTAGGT GGTCTTGACC TCGGCGTCGT AGTGGCCGCC GTCCTTCAGC TTCAGCCTCA
            121 TCTTGATCTC GCCCTTCAGG GCGCCGTCCT CGGGGTACAT CCGCTCGGTG GAGGCCTCCC
            181 AGCCCATGGT CTTCTTCTGC ATTACGGGGC CGTCGGAGGG GAAGTTGGTG CCGCGCAGCT
            241 TCACCTTGTA GATGAACTCG CCGTCCTGCA GGGAGGAGTC CTGGGTCACG GTCACCACGC
            301 CGCCGTCCTC GAAGTTCATC ACGCGCTCCC ACTTGAAGCC CTCGGGGAAG GACAGCTTCA
            361 AGTAGTCGGG GATGTCGGCG GGGTGCTTCA CGTAGGCCTT GGAGCCGTAC TGGAACTGAG
            421 GGGACAGGAT GTCCCAGGCG AAGGGCAGGG GGCCGCCCTT GGTCACCTTC AGCTTGGCGG
            481 TCTGGGTGCC CTCGTAGGGG CGGCCCTCGC CCTCGCCCTC GATCTCGAAC TCGTGGCCGT
            541 TCACGGAGCC CTCCATGCGC ACCTTGAAGC GCATGAACTC CTTGATGACG TCCTCGGANG
            601 ANGNCATCCC GAACGTCTCC TGGGANGCAT AGACCATGTA CNGGAAGCCG TCTTCNTCTC
            661 TCTCGCTCTC NNANNCTTNN NANNNGGGNG NGGNNNNNCT CNCNANGCTG NGNCNNTTCN
          721 NNNNGNANNA ANANNNNNNN NNN
          //
          Primer: IRES-F
          Region: IRES2,PuroR
          Sequence file: RDB19179_B1Fth.seq check
          >14601_19179_B1Ft_2_IRES-F_F03_18_ABI24.ab1
              1 NNNNNNNNNN NNNNNTNNNN CNNNGTTANN AAACGTCTAG GCCCCCCGAA CCACGGGGNC
             61 GTGGTTTTCC TTTGAAAAAC ACGATGATAA TATGGCCACA ACCATGACCG AGTACAAGCC
            121 CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCCGGGCC GTACGCACCC TCGCCGCCGC
            181 GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGACCCG GACCGCCACA TCGAGCGGGT
            241 CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC GACATCGGCA AGGTGTGGGT
            301 CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG GAGAGCGTCG AAGCGGGGGC
            361 GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC GGTTCCCGGC TGGCCGCGCA
            421 GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG GAGCCCGCGT GGTTCCTGGC
            481 CACCGTCGGC GTCTCGCCCG ACCACCAGGG CAAGGGTCTG GGCAGCGCCG TCGTGCTCCC
            541 CGGAGTGNNN GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC CTGGAGACCT CCGCGCCCCG
            601 CAACCNTCCC CCTTCTANNN NNNGCTCGNN NTCACCNGNN NNNGCCNACG TCNAGGTGCC
            661 CGNANNNANN NNGCACNNGN NNNNNNGNNC CNNNNNNNCG GNGNCNNNNG NCCNGNNCNN
          721 NNNNNNNN
          //
          Primer: p15Aori_F
          Region: 3' LTR
          Sequence file: RDB19179_B1Fti.seq check
          >14601_19179_B1Ft_2_p15Aori_F_G03_21_ABI24.ab1
              1 NNNNNNNNNN NNNNNGNNGC TCNTCNNGGG GGCGGNNCCT ATGGNAAAAC GCCAGCAACG
             61 CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT
            121 TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC
            181 GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC
            241 GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAACTATGGC CATTTAATGT
            301 AAATACTTAA GAAAAAAAAC CAAATTAATT TTGATACATG CTGCATGTGA AAGACCCCCG
            361 CTGACGGGTA GTCAATCACT CAGAGGAGAC CCTCCCAAGG AACAGCGAGA CCACAAGTCG
            421 GATGCAACTG CAAGAGGGTT TATTGGATAC ACGGGTACCC GGGCGACTCA GTCAATCGGA
            481 GGACTGGCGC CCCGAGTGAG GGGTTGTGGG CTCTTTTATT GAGCTCGGGG AGCAGAAGCG
            541 CGCGAACAGA AGCGAGAAGC GAACTGATTG GTTAGTTCAA ATAAGGCACA GGGTCATTTC
            601 AGGTCCTTGG GGGCACNCTG GAAACATCTG ATGGNTCTCT AGAAACTGCT GANGGCTGGA
            661 CCGCATCTGG GGANCATCNG NTCTTGGNCN TNNNNCNNGG GNNANNNNNG CNTNANNNCN
          721 NNNATNCNNG NNTNNGNNNC NNNNNTNNNN NNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Morishita, H., A new probe to measure autophagic flux in vitro and in vivo. Autophagy 13 (4): 757-758 (2017). PMID 28121224.
          original Kaizuka, T., An Autophagic Flux Probe that Releases an Internal Control. Mol Cell. 2016 Nov 17;64(4):835-849 (2016). PMID 27818143.
          reference Nguyen, T.B., DGAT1-Dependent Lipid Droplet Biogenesis Protects Mitochondrial Function during Starvation-Induced Autophagy. Dev. Cell 42 (1): 9-21 (2017). PMID 28697336.
          reference Tamura, N., Differential requirement for ATG2A domains for localization to autophagic membranes and lipid droplets. FEBS Lett. 591 (23): 3819-3830 (2017). PMID 29113029.
          user_report Ohnishi, K., Identification of Dietary Phytochemicals Capable of Enhancing the Autophagy Flux in HeLa and Caco-2 Human Cell Lines. Antioxidants (Basel) 9 (12): 1193 (2020). PMID 33261065.

          2022.10.12

          GNP_filter3_RDBDEP_html_221010.pl