RDB14601 - pMRX-IP-GFP-LC3-RFP

Fluorescent probe to evaluate autophagic flux by the rat LC3 fused with GFP and RFP.

Distribution information

Catalog number	RDB14601
Resource name	pMRX-IP-GFP-LC3-RFP
Clone info.	Fluorescent probe to evaluate autophagic flux. Expression vector of rat microtubule-associated protein 1 light chain 3 beta (LC3) fused with GFP and RFP. This probe is cleaved by endogenous ATG4 proteases into equimolar amounts of GFP-LC3 and RFP. The GFP-LC3 is degraded by autophagy, while RFP remains in the cytosol as an internal control. Thus, autophagic flux can be estimated by calculating the GFP/RFP signal ratio. Please refer DISCUSSION section of Kaizuka, T., Mol. Cell 64, 835-849, 2016 for the difference between pMRX-IP-GFP-LC3-RFP-LC3deltaG and this clone. Expression was confirmed by the depositor with immunoblotting and fluorescent microscopy. Please refer Saitoh, T. et al., FEBS Lett., 532 (1-2): 45-51, (2002) for the construction of pMRX-IP vector.
Vector backbone	pMRX-IP (Plasmid)
Selectable markers	Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name	Rat LC3 cDNA Aequorea victoria GFP cDNA Discosoma sp. RFP cDNA
Depositor	Mizushima, Noboru \|

Other clones in our bank	Rat Map1lc3b (KEGG orthology K10435) \|

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	(1) BIOLOGICAL RESOURCE is limited to for academic research in non-profit organization. (2) The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. (3) Any use of the BIOLOGICAL RESOURCE for profit purposes by a non-profit organization or any use of the BIOLOGICAL RESOURCE by a profit organization requires a separate license from the DEPOSITOR prior to distribution. (4) In publishing research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the original publication designated by the DEPOSITOR (Kaizuka, T. et al. Mol. Cell, 64 (4): 835-849, 2016) and an acknowledgment to the DEPOSITOR are requested. (5) The RECIPIENT must contact the DEPOSITOR in case of application for any patents or commercial use with the results from the use of BIOLOGICAL RESOURCE.
Additional terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOUECE, the BIOLOGICAL RESOUECE should be described as pMRX-IP-GFP-LC3-RFP, which has the pMX backbone.
Ordering	Please visit Information of Request for Distribution. Please contact us. UCSD's MTA is also required. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link] Approval form by depositor [Word]
Remarks	((Additional form)) Approval Form(FormD), GFP-A Remember that you will be working with samples containing infectious virus. Please contact us. UCSD's MTA is also required. Please use Schedule A (GFP-A) additionally. Please obtain written approval of the depositor.

Distribution information (domestic users)

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提供条件	1. 非営利団体による学術研究に限る。2. 利用者は事前に寄託者の提供承諾書を得る。3. 当該リソースの非営利団体の営利目的での利用および営利団体の利用については、事前に寄託者との間で、別途使用許諾に係る契約の締結が必要である。4. 研究成果の公表にあたって寄託者の指定する文献(Kaizuka, T. et al. Mol. Cell, 64 (4): 835-849, 2016)を引用し、謝辞の表明を必要とする。5. 利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
付加的提供条件	利用者は、研究成果の公表にあたって次のように記す： pMRX-IP-GFP-LC3-RFP, which has the pMX backbone.
提供依頼	手続きの詳細は、「提供申込みについて」をご覧ください。別途、UCSDとのMTAも必要です。お問い合わせください。提供依頼書 [Word] 提供同意書 [Word] Schedule A（GFP-A, 英語版のみ） [Link] 寄託者による提供承諾書 [Word]
備考	【追加提供依頼書】承諾書(FormD), GFP-A このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。別途、UCSDとのMTAも必要です。お問い合わせください。 Schedule A（GFP-A, 英語版のみ）が追加で必要です。寄託者の書面による提供承諾が必要です。

References and tips

Sequence information

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB14601	pMRX-IP-GFP-LC3-RFP	DNA solution	Please contact us.

Featured content	Autophagy (English text)

original	Morishita, H., A new probe to measure autophagic flux in vitro and in vivo. Autophagy 13 (4): 757-758 (2017). PMID 28121224.
original	Kaizuka, T., An Autophagic Flux Probe that Releases an Internal Control. Mol Cell. 2016 Nov 17;64(4):835-849 (2016). PMID 27818143.

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Primer: EGFP-C	RDB14601_A7Baa.seq
>D04280A2_A7Ba_1_EGFP-C_A05_02_ABI24.ab1 1 ACGGCACTCT CGGCATGGAC GAGCTGTACA AGGGTGGCGG TGGCTCGGGC GGTGGTGGGT 61 CGGGTGGCGG CGGATCTATC GATGAATTGA ATTCCATGCC GTCCGAGAAG ACCTTCAAAC 121 AGCGCCGGAG CTTCGAACAA AGAGTGGAAG ATGTCCGGCT CATCCGGGAG CAGCACCCCA 181 CCAAGATCCC AGTGATTATA GAGCGATACA AGGGTGAGAA GCAGCTGCCC GTCCTGGACA 241 AGACCAAGTT CCTTGTACCT GATCACGTGA ATATGAGCGA ACTCATCAAG ATAATTAGAA 301 GGCGCCTGCA GCTCAATGCT AACCAAGCCT TCTTCCTCCT GGTGAATGGG CACAGCATGG 361 TGAGTGTGTC CACACCCATC TCTGAAGTGT ACGAGAGCGA GAGAGATGAA GACGGCTTCC 421 TGTACATGGT CTATGCCTCC CAGGAGACGT TCGGGATGGC CTCCTCCGAG GACGTCATCA 481 AGGAGTTCAT GCGCTTCAAG GTGCGCATGG AGGGCTCCGT GAACGGCCAC GAGTTCGAGA 541 TCGAGGGCGA GGGCGAGGGC CGCCCCTACG AGGGCACCCA GACCGCCAAG CTGAAGGTGA 601 CCAAGGGCGG CCCCCTGCCC TTCGCCTGGG ACATCCTGTC CCCTCAGTTC CAGTACGGCT 661 CCAAGGCCTA CGTGAAGCAC CCCGCCGACA TCCCCGACTA CTTGAAGCTG TCCTTCCCCG 721 AGGGCTTCAA GTGGGAGCGC GTGATGAACT TCGAGGACGG CGGCGTGGTG ACCGTGACCC 781 AGGACTCCTC CCTGCAGGAC GGCGAGTTCA TCTACAAGGT GAAGCTGCGC GGCACCAACT 841 TCCCCTCCGA CGGCCCCGTA ATGCAGAAGA AGACCATGGG CTGGGAGGCC TCCACCGAGC 901 GGATGTACCC CGAGGACGGC GCCCTGAAAG GGCGAGATCA AGATGAGGCT GAAGCTGAAG 961 ACGGCGGCCA CTACGACGCC GAGGTCAAGA CCACCTACAT GGCCAAGAAG CCCGTGCAGC 1021 TGCCCGGCGC CTACAAGACG ACATCAGCTG GACATCACCT CCCACAACGA GGACTACACA 1081 TCGTGAACAG TACGAGCGCG CCGAGGGCCG CCACTCCACG CGCTAAAGAA TCCAGTGTGG 1141 TGTTACCGAT CCTGCAGCCC TCGAAGGCGG GCCGCCCCCG CCGCCCTACG TAAAATTTCT 1201 CGGC //
Primer: CSII-CMV3'	RDB14601_A7Bab.seq
>D04280A2_A7Ba_1_CSII-CMV3'_B05_05_ABI24.ab1 1 CTCTTACAAG GGGGGGGGGG CGGATTTACG TAGCGGCCGC GGCGCGCCGG CCCTCGAGGC 61 CTGCAGGAAT TCCCGTACCA CCACACTGGG ATCTTTAGGC GCCGGTGGAG TGGCGGCCCT 121 CGGCGCGCTC GTACTGTTCC ACGATGGTGT AGTCCTCGTT GTGGGAGGTG ATGTCCAGCT 181 TGATGTCGGT CTTGTAGGCG CCGGGCAGCT GCACGGGCTT CTTGGCCATG TAGGTGGTCT 241 TGACCTCGGC GTCGTAGTGG CCGCCGTCCT TCAGCTTCAG CCTCATCTTG ATCTCGCCCT 301 TCAGGGCGCC GTCCTCGGGG TACATCCGCT CGGTGGAGGC CTCCCAGCCC ATGGTCTTCT 361 TCTGCATTAC GGGGCCGTCG GAGGGGAAGT TGGTGCCGCG CAGCTTCACC TTGTAGATGA 421 ACTCGCCGTC CTGCAGGGAG GAGTCCTGGG TCACGGTCAC CACGCCGCCG TCCTCGAAGT 481 TCATCACGCG CTCCCACTTG AAGCCCTCGG GGAAGGACAG CTTCAAGTAG TCGGGGATGT 541 CGGCGGGGTG CTTCACGTAG GCCTTGGAGC CGTACTGGAA CTGAGGGGAC AGGATGTCCC 601 AGGCGAAGGG CAGGGGGCCG CCCTTGGTCA CCTTCAGCTT GGCGGTCTGG GTGCCCTCGT 661 AGGGGCGGCC CTCGCCCTCG CCCTCGATCT CGAACTCGTG GCCGTTCACG GAGCCCTCCA 721 TGCGCACCTT GAAGCGCATG AACTCCTTGA TGACGTCCTC GGAGGAGGCC ATCCCGAACG 781 TCTCCTGGGA GGCATAGACC ATGTACAGGA AGCCGTCTTC ATCTCTCTCG CTCTCGTACA 841 CTTCAGAGAT GGGTGTGGAC ACACTCACCA TGCTGTGCCC ATTCACCAGG AGGAAGAGGC 901 TTGGTTTAGC ATTGAGCTGC AGGCGCCTTC TAATTATCTT GATGAGTTCG CTCATATTCA 961 CGTGATCAGG TACAAGGAAC TTGGTCTTGT CCAGGACGGG CAGCTGCTTC TCACCCTTGT 1021 ATCGCTCTAT ATCACTGGGA TCTGGTGGGG GTGCTGCTCC CGGATGAGCC GGACATCTTC 1081 CACTCTTTTG TTCGAAGCTC AGCGCTGTTT GAGTCTTTCT CGGACGGCAT GTAATTCAAT 1141 TCATCGATAG ATTCGTCGCC ACCGACCAAC AACCGCCCGA GAGCCACGGC TAC //
Primer: MMLV-pol_F2	RDB14601_A7Bac.seq
>D04280A2_A7Ba_1_MMLV-pol_F2_C05_08_ABI24.ab1 1 TTCCGGGGGG TCACATCCTC TAGACTGCCG GATCTCCCGC CGCCACCATG GTGAGCAAGG 61 GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG 121 GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC 181 TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC 241 TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT 301 TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG 361 GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG 421 AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA 481 ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC ATCAAGGTGA 541 ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC CACTACCAGC 601 AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC 661 AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG 721 TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGGGTGGC GGTGGCTCGG 781 GCGGTGGTGG GTCGGGTGGC GGCGGATCTA TCGATGAATT GAATTCCATG CCGTCCGAGA 841 AGACCTTCAA ACAGCGCCGG AGCTTCGAAC AAAGAGTGGA AGATGTCCGG CTCATCCGGG 901 AGCAGCACCC CACCAAGATC CCAGTGATTA TAGAGCGATA CAAGGGTGAG AAGCAGCTGC 961 CCGTCCTGAC AGACCAAGTT CCCTTGTACC TGATCACGTG AATATGAGCG AACTCATCAG 1021 ATATTAGACG CGCTGCAGCT CATGCTACCA AGCCTTCCTC TCTGTGATGG GCACAGCATG 1081 GTGGAGTGTG TCCACACCCA TCTCTGAGTG TACGAGAGCC GAGAGAGAAT GGAGACGCAT 1141 CCTGTACATT GATCTTATGC TTCCCCAAGG AGAAACGTTC CGGA //
Primer: EGFP-N	RDB14601_A7Bad.seq
>D04280A2_A7Ba_1_EGFP-N_E05_14_ABI24.ab1 1 GTCACCCGGG GGACGCTCCT CGCCCTTGCT CACCATGGTG GCGGCGGGAG ATCCGGCAGT 61 CTAGAGGATG GTCCACCCCC GGGGTCGGCA GCCTTCACGT GGGCGGCGTG TATCCAAGCT 121 GCGATGCCGT CTACTTTGAG GGCGGTGGGG GTGGTCAGCA GGACTGTGTA AGGTCCTTTC 181 CAGCGAGGTT CTAGGTTCTT AGTCTGGTGT CGGCGGACCC ACACTGTGTC GCCGACTCGG 241 TAAGGGTGAG GTACCACCGG TCGGTCCAGT TGTTCTTGGT AGGCTGCCGC CAGAGGTCTC 301 CAGACTTCGT GCTGGACTAA GTAGAGAGCC TGTAAGTGAG CTTGGAGAGA GGGGCTGTTA 361 GTAACTCTTG TCATGTCAGG GTCAGGGAAG TTTACAAGGG GCGGGGGTGC CCCATATAAG 421 ATCTCATATG GCCATATGGG GGCGCCTAGA GAAGGAGTGA GGGCTGGATA AAGGGAGGAT 481 CGAGGCGGGG TCGAACGAGG AGGTTCAAGG GGGAGAGACG GGGCGGATGG AGGAAGAGGA 541 GGCGGAGGCT TAGGGTGTAC AAAGGGCTTG ACCCAGGGAG GGGGGTCAAA AGCCAAGGCT 601 TCCCAGGTCA CGATGTAGGG GACCTGGTCT GGGTGTCCAT GCGGGCCAGG TGAAAAGACC 661 TTGATCTTAA CCTGGGTGAT GAGGTCTCGG TTAAAGGTGC CGTCTCGCGG CCATCCGACG 721 TTAAAGGTTG GCCATTCTGC AGAGCAGAAG GTAACCCAAC GTCTCTTCTT GACATCTACC 781 GACTGGTTGT GAGCGATCCG CTCGACATCT TTCCAGTGAC CTAAGGTCAA ACTTAAGGGA 841 GTGGTAACAG TCTGGCCCGG GCCCATATTT TCAGACAAAT ACAGAAACAC AGTCAGACAG 901 AGACAACACA GAACGATGCT GCAGCAGACA AGACGCGCGG CGCGGCTTCG GTCCCAAACC 961 GAAAGCAAAA ATTCAGACGG AGGCGGGAAC TGTTTTAGGT TCTCGTCTCC TACCAGAACC 1021 ACATATCCCT CCTCTAAGGG GGGTGCACCA AAGAGTCCAA ACGATCGGGA TTTTTGGACT 1081 CAGTCGGCAC AAAAACCGAC CCCGAGTCCT GGACGTCTCC AGGCTTGCGC CGCGTCCGAC 1141 TCGTCAGTCC ACCACGGGTT CCGGCCGGAA TTAG //
Primer: pJB8-Forward	RDB14601_A7Bae.seq
>D04280A2_A7Ba_1_pJB8-Forward_D05_11_ABI24.ab1 1 GGCGTGCCAT TGGCAATACG TTGTATCCAT ATCATAATAT GTACATTTAT ATTGGCTCAT 61 GTCCAACATT ACCGCCATGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 121 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 181 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 241 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 301 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 361 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 421 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GCTAGAGAAC CATCAGATGT 481 TTCCAGGGTG CCCCAAGGAC CTGAAATGAC CCTGTGCCTT ATTTGAACTA ACCAATCAGT 541 TCGCTTCTCG CTTCTGTTCG CGCGCTTCTG CTCCCCGAGC TCAATAAAAG AGCCCACAAC 601 CCCTCACTCG GCGCGCCAGT CCTCCGATTG ACTGAGTCGC CCGGGTACCC GTGTATCCAA 661 TAAACCCTCT TGCAGTTGCA TCCGACTTGT GGTCTCGCTG TTCCTTGGGA GGGTCTCCTC 721 TGAGTGATTG ACTACCCGTC AGCGGGGGTC TTTCATTTGG GGGCTCGTCC GGGATCGGGA 781 GACCCCTGCC CAGGGACCAC CGACCCACCA CCGGGAGGTA AGCTGGCCAG CAACTTATCT 841 GTGTCTGTCC GATTGTCTAG TGTCTATGAC TGATTTTATG CGCCTGCGTC GGTACTAGTT 901 AGCTAACTAG CTCTGTATCT GGCGGACCCG TGGTGGAACT GACGAGTTCG GAACACCCGG 961 GCCGCAACCC TGGGAGACGT CCCAGGACTT CGGGGGCCGT TTTTGTGGCC CGACCTGAGT 1021 CCAAAATCCC GATCGTTTTG GACTCTTTGG TGCACCCCCC CTTAGAGAGG ATATGTGGAT 1081 CTGTAGAGAC GGAGACCTAA CAGTTCCCGC CTCCGGTCTG AATTTGCTTC GGATTGGAAC 1141 GAAGCCGCGC CGCGCGTCTT GTCTTGCCTG CCAAGCCAAC TCG //
Primer: p15Aori_F	RDB14601_A7Baf.seq
>D04280A2_A7Ba_1_p15Aori_F_F05_17_ABI24.ab1 1 GCAGGGGCCG TGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG 61 CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC 121 TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC 181 TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC 241 AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAACT ATGGCCATTT 301 AATGTAAATA CTTAAGAAAA AAAACCAAAT TAATTTTGAT ACATGCTGCA TGTGAAAGAC 361 CCCCGCTGAC GGGTAGTCAA TCACTCAGAG GAGACCCTCC CAAGGAACAG CGAGACCACA 421 AGTCGGATGC AACTGCAAGA GGGTTTATTG GATACACGGG TACCCGGGCG ACTCAGTCAA 481 TCGGAGGACT GGCGCCCCGA GTGAGGGGTT GTGGGCTCTT TTATTGAGCT CGGGGAGCAG 541 AAGCGCGCGA ACAGAAGCGA GAAGCGAACT GATTGGTTAG TTCAAATAAG GCACAGGGTC 601 ATTTCAGGTC CTTGGGGCAC CCTGGAAACA TCTGATGGTT CTCTAGAAAC TGCTGAGGGC 661 TGGACCGCAT CTGGGGACCA TCTGTTCTTG GCCCTGAGCC GGGGCAGGAA CTGCTTACCA 721 CAGATATCCT GTTTGGCCCA TATTCAGCTG TTCCATCTGT TCTTGGCCCT GAGCCGGGGC 781 AGGAACTGCT TACCACAGAT ATCCTGTTTG GCCCATATTC AGCTGTTCCA TCTGTTCCTG 841 ACCTTGATCT GAACTTCTCT ATTCTCAGTT ATGTATTTTT CCATGCCTTG CAAAATGGCG 901 TTACTTAAGC TAGCTTGCCA AACCTACAGG TGGGGTCTTT CATTCCCCCC TTTTTCTGGA 961 GACTAAATAA AATCTTTTAT TTTATCGTCG AGCCATGGGG GTCGTGCGCT CCTTTCGGGT 1021 CGGGCGCTGC GGGTCGTGGG GCGGGCGTCA GGCACCGGGG CTTGCGGGTC ATGCACAGTG 1081 CGCGGTCCTT CGGCACTCGA CGTCGGCGTT GACGGTGAGC GAGCCGCTCG TAGAGGTAGG 1141 TGCCGGCGCG GCAGTCTCAG TAGCGATCAC TCCGGGGAG //
Primer: pMXs-3LTRup_R2	RDB14601_A7Bag.seq
>D04280A2_A7Ba_1_pMXs-3LTRup_R2_G05_20_ABI24.ab1 1 GGCATGTAAA TCTTTTATTT TATCGTCGAG CCATGGGGTC GTGCGCTCCT TTCGGTCGGG 61 CGCTGCGGGT CGTGGGGCGG GCGTCAGGCA CCGGGCTTGC GGGTCATGCA CCAGGTGCGC 121 GGTCCTTCGG GCACCTCGAC GTCGGCGGTG ACGGTGAAGC CGAGCCGCTC GTAGAAGGGG 181 AGGTTGCGGG GCGCGGAGGT CTCCAGGAAG GCGGGCACCC CGGCGCGCTC GGCCGCCTCC 241 ACTCCGGGGA GCACGACGGC GCTGCCCAGA CCCTTGCCCT GGTGGTCGGG CGAGACGCCG 301 ACGGTGGCCA GGAACCACGC GGGCTCCTTG GGCCGGTGCG GCGCCAGGAG GCCTTCCATC 361 TGTTGCTGCG CGGCCAGCCG GGAACCGCTC AACTCGGCCA TGCGCGGGCC GATCTCGGCG 421 AACACCGCCC CCGCTTCGAC GCTCTCCGGC GTGGTCCAGA CCGCCACCGC GGCGCCGTCG 481 TCCGCGACCC ACACCTTGCC GATGTCGAGC CCGACGCGCG TGAGGAAGAG TTCTTGCAGC 541 TCGGTGACCC GCTCGATGTG GCGGTCCGGG TCGACGGTGT GGCGCGTGGC GGGGTAGTCG 601 GCGAACGCGG CGGCGAGGGT GCGTACGGCC CGGGGGACGT CGTCGCGGGT GGCGAGGCGC 661 ACCGTGGGCT TGTACTCGGT CATGGTTGTG GCCATATTAT CATCGTGTTT TTCAAAGGAA 721 AACCACGTCC CCGTGGTTCG GGGGGCCTAG ACGTTTTTTT AACCTCGACT AAACACATGT 781 AAAGCATGTG CACCGAGGCC CCAGATCAGA TCCCATACAA TGGGGTACCT TCTGGGCATC 841 CTTCAGCCCC TTGTTGAATA CGCTTGAGGA GAGCCATTTG ACTCTTTCCA CAACTATCCA 901 ACTCACAACG TGGCACTGGG GTTGTGCCGC CTTTGCAGTG TATCTTATAC ACGTGGCTTT 961 TGGCCGCAGA GGCACCTGTC GCCAGGTGGG GGGGTTTCCG CTGCCTGCAA AGGGTCGCTA 1021 CAGACGTTGT TTGTCTCAGA GCTTCCAGAG GACTGCTTCA TCACGACATT CACAGACTTG 1081 CATTCATGCG AGAGGGGAAG ACCCCTAGAT GCTCGTCAGA GACCGTCAGG CTTCGGCCTC 1141 ACATGTGCCA AAAGAACGGC CA //