Fluorescent probe to evaluate autophagic flux by the rat LC3 fused with GFP and RFP.
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Other clones in our bank
External Database Rat LC3 Aequorea victoria GFP Reference sequence |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution | (1) BIOLOGICAL RESOURCE is limited to for academic research in non-profit organization. (2) The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. (3) Any use of the BIOLOGICAL RESOURCE for profit purposes by a non-profit organization or any use of the BIOLOGICAL RESOURCE by a profit organization requires a separate license from the DEPOSITOR prior to distribution. (4) In publishing research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the original publication designated by the DEPOSITOR (Kaizuka, T. et al. Mol. Cell, 64 (4): 835-849, 2016) and an acknowledgment to the DEPOSITOR are requested. (5) The RECIPIENT must contact the DEPOSITOR in case of application for any patents or commercial use with the results from the use of BIOLOGICAL RESOURCE. Additional terms and conditions: Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested. |
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Ordering | Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment ![]() ![]() Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word ![]() Approval form by depositor [Word ![]() |
Remarks | ((Additional form)) Approval Form(FormD) Remember that you will be working with samples containing infectious virus. This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. Please obtain written approval of the depositor. |
提供条件 | 1. 非営利団体による学術研究に限る。2. 利用者は事前に寄託者の提供承諾書を得る。3. 当該リソースの非営利団体の営利目的での利用および営利団体の利用については、事前に寄託者との間で、別途使用許諾に係る契約の締結が必要である。4. 研究成果の公表にあたって寄託者の指定する文献(Kaizuka, T. et al. Mol. Cell, 64 (4): 835-849, 2016)を引用し、謝辞の表明を必要とする。5. 利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。 付加的提供条件: pMRXベクターについて:利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。 |
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提供依頼 | 手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word ![]() 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word ![]() 寄託者による提供承諾書 [Word ![]() |
備考 | 【追加提供依頼書】承諾書(FormD) このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。 寄託者の書面による提供承諾が必要です。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB14601 | pMRX-IP-GFP-LC3-RFP | DNA solution |
Materials & Methods section:
The pMRX-IP-GFP-LC3-RFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14601). |
Reference section:
Further references such as user reports and related articles (go to bottom)
Featured content
Featured content | Autophagy (English text) |
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RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB19179_B1Ftp1-1.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: MMLV-pol_F (Pr0352) Region: pol region,insert 5'(EGFP 5') Sequence file: RDB19179_B1Fta.seq ![]() |
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>14601_19179_B1Ft_2_MMLV-pol_F_150_E02_14_ABI24.ab1 1 NNNNNNNNNN NNGGNATCGN AGCTTGGNNA CNCGCCGCCC ACGTGAAGGC TGCCGACCCC 61 GGGGGTGGAC CATCCTCTAG ACTGCCGGAT CTCCCGCCGC CACCATGGTG AGCAAGGGCG 121 AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC 181 ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA 241 AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCCTGA 301 CCTACGGCGT GCAGTGCTTC AGCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA 361 AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG GACGACGGCA 421 ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC CGCATCGAGC 481 TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG GAGTACAACT 541 ACAACAGCCA CAACGTCTAT ATCATGGCCG ACAAGCAGAA GAACGGCATC AAGGTGAACT 601 TCAAGATCCG CCACAACATC GAGGACGGCA GCGTGCAGCT CGCCGANCAC TACCAGCAGA 661 ACACCCCNAT CNGCGANGGC CCNNNGCTGC TGCCNGANNN CCNCTNNNNN ANCNNNNNNN 721 NNCNNNNNNN ANNNNCCNNN NNNNNNN // |
Primer: mRFP1-inner1 (Pr0443) Region: insert 3'(mRFP1 3'),IRES2 Sequence file: RDB19179_B1Ftb.seq ![]() |
>14601_19179_B1Ft_2_mRFP1-inner1_C03_09_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNTCCTCC CNGNNNGNNG GCNNNTTCAT CTACAAGGTG 61 AAGCTGCGCG GCACCAACTT CCCCTCCGAC GGCCCCGTAA TGCAGAAGAA GACCATGGGC 121 TGGGAGGCCT CCACCGAGCG GATGTACCCC GAGGACGGCG CCCTGAAGGG CGAGATCAAG 181 ATGAGGCTGA AGCTGAAGGA CGGCGGCCAC TACGACGCCG AGGTCAAGAC CACCTACATG 241 GCCAAGAAGC CCGTGCAGCT GCCCGGCGCC TACAAGACCG ACATCAAGCT GGACATCACC 301 TCCCACAACG AGGACTACAC CATCGTGGAA CAGTACGAGC GCGCCGAGGG CCGCCACTCC 361 ACCGGCGCCT AAAGATCCCA GTGTGGTGGT ACGGGAATTC CTGCAGGCCT CGAGGGCCGG 421 CGCGCCGCGG CCGCTACGTA AATTCCGCCC CCCCCCCCCC TCTCCCNNNN NNNNNCCTAA 481 CGTTNNNNNN CGAANCCNCT TGNANNANNN NNGGTGTGCG TTTGTCTNNN NNNTATTTNN 541 NNNNNNNN // |
Primer: mVenus-M_F (Pr0574) Region: insert mid(EGFP,rat LC3) Sequence file: RDB19179_B1Ftc.seq ![]() |
>14601_19179_B1Ft_2_mVenus-M_F_B03_06_ABI24.ab1 1 NNNNNNNNNN NNNGGNNTNN GGNNNANTTN ANGNTCCGCC ACAACATCGA GGNNGGCAGC 61 GTGCAGCTCG CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG 121 CCCGACAACC ACTACCTGAG CACCCAGTCC GCCCTGAGCA AAGACCCCAA CGAGAAGCGC 181 GATCACATGG TCCTGCTGGA GTTCGTGACC GCCGCCGGGA TCACTCTCGG CATGGACGAG 241 CTGTACAAGG GTGGCGGTGG CTCGGGCGGT GGTGGGTCGG GTGGCGGCGG ATCTATCGAT 301 GAATTGAATT CCATGCCGTC CGAGAAGACC TTCAAACAGC GCCGGAGCTT CGAACAAAGA 361 GTGGAAGATG TCCGGCTCAT CCGGGAGCAG CACCCCACCA AGATCCCAGT GATTATAGAG 421 CGATACAAGG GTGAGAAGCA GCTGCCCGTC CTGGACAAGA CCAAGTTCCT TGTACCTGAT 481 CACGTGAATA TGAGCGAACT CATCAAGATA ATTAGAAGGC GCCTGCAGCT CAATGCTAAC 541 CAAGCCTTCT TCCTCCTGGT GAATGGGCAC AGCATGGTGA GTGTGTCCNC ACCCATCTCT 601 GAAGTGTACG AGAGCGAGAG AGANGAAGAC GGCTTCCTGT ACNTGGNCNN NGNCNTCCNN 661 GGAGNNGTTC GGNANNNNCN TCCTCCGANG NNNGTCNTNN NNNNNNTNNN NGNNNNNNNN 721 NN // |
Primer: mCherry-check_R (Pr0521) Region: insert mid(mRFP1,rat LC3) Sequence file: RDB19179_B1Ftd.seq ![]() |
>14601_19179_B1Ft_2_mCharry-check_R_E03_15_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNTCC TCNNNNTTCN TCACGCGCTC CCACTTGAAG 61 CCCTCGGGGA AGGACAGCTT CAAGTAGTCG GGGNTGTCGG CGGGGTGCTT CACGTAGGCC 121 TTGGAGCCGT ACTGGAACTG AGGGGACAGG ATGTCCCAGG CGAAGGGCAG GGGGCCGCCC 181 TTGGTCACCT TCAGCTTGGC GGTCTGGGTG CCCTCGTAGG GGCGGCCCTC GCCCTCGCCC 241 TCGATCTCGA ACTCGTGGCC GTTCACGGAG CCCTCCATGC GCACCTTGAA GCGCATGAAC 301 TCCTTGATGA CGTCCTCGGA GGAGGCCATC CCGAACGTCT CCTGGGAGGC ATAGACCATG 361 TACAGGAAGC CGTCTTCATC TCTCTCGCTC TCGTACACTT CAGAGATGGG TGTGGACACA 421 CTCACCATGC TGTGCCCATT CACCAGGAGG AAGAAGGCTT GGTTAGCATT GAGCTGCAGG 481 CGCCTTCTAA TTATCTTGAT GAGTTCGCTC ATATTCACGT GATCAGGTAC AAGGAACTTG 541 GTCTTGTCCA GGACGGGCAG CTGCTTCTCA NCCTTGTATC GCTCTATAAT CACTGGGATC 601 TTGGTGGGGT GCTGCTCCCG GNTGAGCCNG GACATCTTCC NCTCTTTGNT CGAANCTCNN 661 GCGCTGTTTG ANNGNCTTCT CNNACNNNNN NNNNN // |
Primer: gag-N_R (Pr0745) Region: MMLV psi, R-U5 Sequence file: RDB19179_B1Fte.seq ![]() |
>14601_19179_B1Ft_2_gag-N_R_C02_08_ABI24.ab1 1 NNNNNNNNNN NNCNGGCCNG GGCCNNTATT TTNNGACAAA TACAGAAACA CAGTCAGACA 61 GAGACAACAC AGAACGATGC TGCAGCAGAC AAGACGCGCG GCGCGGCTTC GGTCCCAAAC 121 CGAAAGCAAA AATTCAGACG GAGGCGGGAA CTGTTTTAGG TTCTCGTCTC CTACCAGAAC 181 CACATATCCC TCCTCTAAGG GGGGTGCACC AAAGAGTCCA AAACGATCGG GATTTTTGGA 241 CTCAGGTCGG GCCACAAAAA CGGCCCCCGA AGTCCCTGGG ACGTCTCCCA GGGTTGCGGC 301 CGGGTGTTCC GAACTCGTCA GTTCCACCAC GGGTCCGCCA GATACAGAGC TAGTTAGCTA 361 ACTAGTACCG ACGCAGGCGC ATAAAATCAG TCATAGACAC TAGACAATCG GACAGACACA 421 GATAAGTTGC TGGCCAGCTT ACCTCCCGGT GGTGGGTCGG TGGTCCCTGG GCAGGGGTCT 481 CCCGATCCCG GACGAGCCCC CAAATGAAAG ACCCCCGCTG ACGGGTAGTC AATCACTCAG 541 AGGAGACCCT CCCNAGGAAC AGCGAGACCA CAAGTCGGAT GCAACTGCAA GAGGGTTTAT 601 TGGATACACG GGTANCCGGG CGANTCNGTC AATCGNANGA NTGGCGCGCC NANTGAGGGG 661 NTGTGGGCTC NTTTATTGAN NTCGGGGANC ANAANCGCGC GANNNGAANC NANNNCGAAN 721 NGANNNNNNN NNN // |
Primer: pMFG_1 (Pr0230) Region: pol region,gag Sequence file: RDB19179_B1Ftf.seq ![]() |
>14601_19179_B1Ft_2_pMFG_1_D03_12_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNTCTNGT NNTGTCAGGG TCAGGGNAGT TTACAAGGGG 61 CGGGGGTGCC CCATATAAGA TCTCATATGG CCATATGGGG GCGCCTAGAG AAGGAGTGAG 121 GGCTGGATAA AGGGAGGATC GAGGCGGGGT CGAACGAGGA GGTTCAAGGG GGAGAGACGG 181 GGCGGATGGA GGAAGAGGAG GCGGAGGCTT AGGGTGTACA AAGGGCTTGA CCCAGGGAGG 241 GGGGTCAAAA GCCAAGGCTT CCCAGGTCAC GATGTAGGGG ACCTGGTCTG GGTGTCCATG 301 CGGGCCAGGT GAAAAGACCT TGATCTTAAC CTGGGTGATG AGGTCTCGGT TAAAGGTGCC 361 GTCTCGCGGC CATCCGACGT TAAAGGTTGG CCATTCTGCA GAGCAGAAGG TAACCCAACG 421 TCTCTTCTTG ACATCTACCG ACTGGTTGTG AGCGATCCGC TCGACATCTT TCCAGTGACC 481 TAAGGTCAAA CTTAAGGGAG TGGTAACAGT CTGGCCCGGG CCCATATTTT CAGACAAATA 541 CAGAAACACA GTCAGACAGA GACNACACAG AACGATGCTG CAGCAGACAA GACGCGCGGC 601 GCGGCTTCNG TCCCAAACCG AAAGCAAAAA TTCNGANGGA GGCGGGAACT GNTTTAGNNT 661 CTCGTCTCCT ACCAGANNCA CATATCCCTN CNTCTNANNG GGGGNGNANN NNNNNANTNN 721 NNAANNNNTN NNNNNNNTTN TNNNNNNNNN N // |
Primer: mCherry-C_R (Pr0517) Region: insert mid(mRFP1) Sequence file: RDB19179_B1Ftg.seq ![]() |
>14601_19179_B1Ft_2_mCherry-C_R_C03_09_ABI24.ab1 1 NNNNNNNNNN NNNCNNNNNG TCGGTCTTGT AGGCGCCGGG CAGCTGCACG GGCTTCTTGG 61 CCATGTAGGT GGTCTTGACC TCGGCGTCGT AGTGGCCGCC GTCCTTCAGC TTCAGCCTCA 121 TCTTGATCTC GCCCTTCAGG GCGCCGTCCT CGGGGTACAT CCGCTCGGTG GAGGCCTCCC 181 AGCCCATGGT CTTCTTCTGC ATTACGGGGC CGTCGGAGGG GAAGTTGGTG CCGCGCAGCT 241 TCACCTTGTA GATGAACTCG CCGTCCTGCA GGGAGGAGTC CTGGGTCACG GTCACCACGC 301 CGCCGTCCTC GAAGTTCATC ACGCGCTCCC ACTTGAAGCC CTCGGGGAAG GACAGCTTCA 361 AGTAGTCGGG GATGTCGGCG GGGTGCTTCA CGTAGGCCTT GGAGCCGTAC TGGAACTGAG 421 GGGACAGGAT GTCCCAGGCG AAGGGCAGGG GGCCGCCCTT GGTCACCTTC AGCTTGGCGG 481 TCTGGGTGCC CTCGTAGGGG CGGCCCTCGC CCTCGCCCTC GATCTCGAAC TCGTGGCCGT 541 TCACGGAGCC CTCCATGCGC ACCTTGAAGC GCATGAACTC CTTGATGACG TCCTCGGANG 601 ANGNCATCCC GAACGTCTCC TGGGANGCAT AGACCATGTA CNGGAAGCCG TCTTCNTCTC 661 TCTCGCTCTC NNANNCTTNN NANNNGGGNG NGGNNNNNCT CNCNANGCTG NGNCNNTTCN 721 NNNNGNANNA ANANNNNNNN NNN // |
Primer: IRES-F (Pr0036) Region: IRES2,PuroR Sequence file: RDB19179_B1Fth.seq ![]() |
>14601_19179_B1Ft_2_IRES-F_F03_18_ABI24.ab1 1 NNNNNNNNNN NNNNNTNNNN CNNNGTTANN AAACGTCTAG GCCCCCCGAA CCACGGGGNC 61 GTGGTTTTCC TTTGAAAAAC ACGATGATAA TATGGCCACA ACCATGACCG AGTACAAGCC 121 CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCCGGGCC GTACGCACCC TCGCCGCCGC 181 GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGACCCG GACCGCCACA TCGAGCGGGT 241 CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC GACATCGGCA AGGTGTGGGT 301 CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG GAGAGCGTCG AAGCGGGGGC 361 GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC GGTTCCCGGC TGGCCGCGCA 421 GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG GAGCCCGCGT GGTTCCTGGC 481 CACCGTCGGC GTCTCGCCCG ACCACCAGGG CAAGGGTCTG GGCAGCGCCG TCGTGCTCCC 541 CGGAGTGNNN GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC CTGGAGACCT CCGCGCCCCG 601 CAACCNTCCC CCTTCTANNN NNNGCTCGNN NTCACCNGNN NNNGCCNACG TCNAGGTGCC 661 CGNANNNANN NNGCACNNGN NNNNNNGNNC CNNNNNNNCG GNGNCNNNNG NCCNGNNCNN 721 NNNNNNNN // |
Primer: p15Aori_F (Pr0276) Region: 3' LTR Sequence file: RDB19179_B1Fti.seq ![]() |
>14601_19179_B1Ft_2_p15Aori_F_G03_21_ABI24.ab1 1 NNNNNNNNNN NNNNNGNNGC TCNTCNNGGG GGCGGNNCCT ATGGNAAAAC GCCAGCAACG 61 CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT 121 TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC 181 GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC 241 GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAACTATGGC CATTTAATGT 301 AAATACTTAA GAAAAAAAAC CAAATTAATT TTGATACATG CTGCATGTGA AAGACCCCCG 361 CTGACGGGTA GTCAATCACT CAGAGGAGAC CCTCCCAAGG AACAGCGAGA CCACAAGTCG 421 GATGCAACTG CAAGAGGGTT TATTGGATAC ACGGGTACCC GGGCGACTCA GTCAATCGGA 481 GGACTGGCGC CCCGAGTGAG GGGTTGTGGG CTCTTTTATT GAGCTCGGGG AGCAGAAGCG 541 CGCGAACAGA AGCGAGAAGC GAACTGATTG GTTAGTTCAA ATAAGGCACA GGGTCATTTC 601 AGGTCCTTGG GGGCACNCTG GAAACATCTG ATGGNTCTCT AGAAACTGCT GANGGCTGGA 661 CCGCATCTGG GGANCATCNG NTCTTGGNCN TNNNNCNNGG GNNANNNNNG CNTNANNNCN 721 NNNATNCNNG NNTNNGNNNC NNNNNTNNNN NNNNNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Morishita, H., A new probe to measure autophagic flux in vitro and in vivo. Autophagy 13 (4): 757-758 (2017). PMID 28121224. |
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original | Kaizuka, T., An Autophagic Flux Probe that Releases an Internal Control. Mol Cell. 2016 Nov 17;64(4):835-849 (2016). PMID 27818143. |
reference | Nguyen, T.B., DGAT1-Dependent Lipid Droplet Biogenesis Protects Mitochondrial Function during Starvation-Induced Autophagy. Dev. Cell 42 (1): 9-21 (2017). PMID 28697336. |
reference | Tamura, N., Differential requirement for ATG2A domains for localization to autophagic membranes and lipid droplets. FEBS Lett. 591 (23): 3819-3830 (2017). PMID 29113029. |
user_report | Ohnishi, K., Identification of Dietary Phytochemicals Capable of Enhancing the Autophagy Flux in HeLa and Caco-2 Human Cell Lines. Antioxidants (Basel) 9 (12): 1193 (2020). PMID 33261065. |
2023.05.22
GNP_filter3_RDBDEP_html_230512.pl