Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMRX-IP-GFP-LC3-RFP

Fluorescent probe to evaluate autophagic flux by the rat LC3 fused with GFP and RFP.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	(1) BIOLOGICAL RESOURCE is limited to for academic research in non-profit organization. (2) The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. (3) Any use of the BIOLOGICAL RESOURCE for profit purposes by a non-profit organization or any use of the BIOLOGICAL RESOURCE by a profit organization requires a separate license from the DEPOSITOR prior to distribution. (4) In publishing research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the original publication designated by the DEPOSITOR (Kaizuka, T. et al. Mol. Cell, 64 (4): 835-849, 2016) and an acknowledgment to the DEPOSITOR are requested. (5) The RECIPIENT must contact the DEPOSITOR in case of application for any patents or commercial use with the results from the use of BIOLOGICAL RESOURCE. Additional terms and conditions: Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form by depositor [Word]
Remarks	((Additional form)) Approval Form(FormD) Remember that you will be working with samples containing infectious virus. This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. Please obtain written approval of the depositor.

提供案内 (日本国内) [open/close]

提供条件	1. 非営利団体による学術研究に限る。2. 利用者は事前に寄託者の提供承諾書を得る。3. 当該リソースの非営利団体の営利目的での利用および営利団体の利用については、事前に寄託者との間で、別途使用許諾に係る契約の締結が必要である。4. 研究成果の公表にあたって寄託者の指定する文献(Kaizuka, T. et al. Mol. Cell, 64 (4): 835-849, 2016)を引用し、謝辞の表明を必要とする。5. 利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。 付加的提供条件： pMRXベクターについて：利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 寄託者による提供承諾書 [Word]
備考	【追加提供依頼書】承諾書(FormD) このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。 寄託者の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB19179_B1Ftp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352) Region: pol region,insert 5'(EGFP 5') Sequence file: RDB19179_B1Fta.seq
>14601_19179_B1Ft_2_MMLV-pol_F_150_E02_14_ABI24.ab1     1 NNNNNNNNNN NNGGNATCGN AGCTTGGNNA CNCGCCGCCC ACGTGAAGGC TGCCGACCCC    61 GGGGGTGGAC CATCCTCTAG ACTGCCGGAT CTCCCGCCGC CACCATGGTG AGCAAGGGCG   121 AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC   181 ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA   241 AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCCTGA   301 CCTACGGCGT GCAGTGCTTC AGCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA   361 AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG GACGACGGCA   421 ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC CGCATCGAGC   481 TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG GAGTACAACT   541 ACAACAGCCA CAACGTCTAT ATCATGGCCG ACAAGCAGAA GAACGGCATC AAGGTGAACT   601 TCAAGATCCG CCACAACATC GAGGACGGCA GCGTGCAGCT CGCCGANCAC TACCAGCAGA   661 ACACCCCNAT CNGCGANGGC CCNNNGCTGC TGCCNGANNN CCNCTNNNNN ANCNNNNNNN 721 NNCNNNNNNN ANNNNCCNNN NNNNNNN //
Primer: mRFP1-inner1 (Pr0443) Region: insert 3'(mRFP1 3'),IRES2 Sequence file: RDB19179_B1Ftb.seq
>14601_19179_B1Ft_2_mRFP1-inner1_C03_09_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNTCCTCC CNGNNNGNNG GCNNNTTCAT CTACAAGGTG    61 AAGCTGCGCG GCACCAACTT CCCCTCCGAC GGCCCCGTAA TGCAGAAGAA GACCATGGGC   121 TGGGAGGCCT CCACCGAGCG GATGTACCCC GAGGACGGCG CCCTGAAGGG CGAGATCAAG   181 ATGAGGCTGA AGCTGAAGGA CGGCGGCCAC TACGACGCCG AGGTCAAGAC CACCTACATG   241 GCCAAGAAGC CCGTGCAGCT GCCCGGCGCC TACAAGACCG ACATCAAGCT GGACATCACC   301 TCCCACAACG AGGACTACAC CATCGTGGAA CAGTACGAGC GCGCCGAGGG CCGCCACTCC   361 ACCGGCGCCT AAAGATCCCA GTGTGGTGGT ACGGGAATTC CTGCAGGCCT CGAGGGCCGG   421 CGCGCCGCGG CCGCTACGTA AATTCCGCCC CCCCCCCCCC TCTCCCNNNN NNNNNCCTAA   481 CGTTNNNNNN CGAANCCNCT TGNANNANNN NNGGTGTGCG TTTGTCTNNN NNNTATTTNN 541 NNNNNNNN //
Primer: mVenus-M_F (Pr0574) Region: insert mid(EGFP,rat LC3) Sequence file: RDB19179_B1Ftc.seq
>14601_19179_B1Ft_2_mVenus-M_F_B03_06_ABI24.ab1     1 NNNNNNNNNN NNNGGNNTNN GGNNNANTTN ANGNTCCGCC ACAACATCGA GGNNGGCAGC    61 GTGCAGCTCG CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG   121 CCCGACAACC ACTACCTGAG CACCCAGTCC GCCCTGAGCA AAGACCCCAA CGAGAAGCGC   181 GATCACATGG TCCTGCTGGA GTTCGTGACC GCCGCCGGGA TCACTCTCGG CATGGACGAG   241 CTGTACAAGG GTGGCGGTGG CTCGGGCGGT GGTGGGTCGG GTGGCGGCGG ATCTATCGAT   301 GAATTGAATT CCATGCCGTC CGAGAAGACC TTCAAACAGC GCCGGAGCTT CGAACAAAGA   361 GTGGAAGATG TCCGGCTCAT CCGGGAGCAG CACCCCACCA AGATCCCAGT GATTATAGAG   421 CGATACAAGG GTGAGAAGCA GCTGCCCGTC CTGGACAAGA CCAAGTTCCT TGTACCTGAT   481 CACGTGAATA TGAGCGAACT CATCAAGATA ATTAGAAGGC GCCTGCAGCT CAATGCTAAC   541 CAAGCCTTCT TCCTCCTGGT GAATGGGCAC AGCATGGTGA GTGTGTCCNC ACCCATCTCT   601 GAAGTGTACG AGAGCGAGAG AGANGAAGAC GGCTTCCTGT ACNTGGNCNN NGNCNTCCNN   661 GGAGNNGTTC GGNANNNNCN TCCTCCGANG NNNGTCNTNN NNNNNNTNNN NGNNNNNNNN 721 NN //
Primer: mCherry-check_R (Pr0521) Region: insert mid(mRFP1,rat LC3) Sequence file: RDB19179_B1Ftd.seq
>14601_19179_B1Ft_2_mCharry-check_R_E03_15_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNTCC TCNNNNTTCN TCACGCGCTC CCACTTGAAG    61 CCCTCGGGGA AGGACAGCTT CAAGTAGTCG GGGNTGTCGG CGGGGTGCTT CACGTAGGCC   121 TTGGAGCCGT ACTGGAACTG AGGGGACAGG ATGTCCCAGG CGAAGGGCAG GGGGCCGCCC   181 TTGGTCACCT TCAGCTTGGC GGTCTGGGTG CCCTCGTAGG GGCGGCCCTC GCCCTCGCCC   241 TCGATCTCGA ACTCGTGGCC GTTCACGGAG CCCTCCATGC GCACCTTGAA GCGCATGAAC   301 TCCTTGATGA CGTCCTCGGA GGAGGCCATC CCGAACGTCT CCTGGGAGGC ATAGACCATG   361 TACAGGAAGC CGTCTTCATC TCTCTCGCTC TCGTACACTT CAGAGATGGG TGTGGACACA   421 CTCACCATGC TGTGCCCATT CACCAGGAGG AAGAAGGCTT GGTTAGCATT GAGCTGCAGG   481 CGCCTTCTAA TTATCTTGAT GAGTTCGCTC ATATTCACGT GATCAGGTAC AAGGAACTTG   541 GTCTTGTCCA GGACGGGCAG CTGCTTCTCA NCCTTGTATC GCTCTATAAT CACTGGGATC   601 TTGGTGGGGT GCTGCTCCCG GNTGAGCCNG GACATCTTCC NCTCTTTGNT CGAANCTCNN 661 GCGCTGTTTG ANNGNCTTCT CNNACNNNNN NNNNN //
Primer: gag-N_R (Pr0745) Region: MMLV psi, R-U5 Sequence file: RDB19179_B1Fte.seq
>14601_19179_B1Ft_2_gag-N_R_C02_08_ABI24.ab1     1 NNNNNNNNNN NNCNGGCCNG GGCCNNTATT TTNNGACAAA TACAGAAACA CAGTCAGACA    61 GAGACAACAC AGAACGATGC TGCAGCAGAC AAGACGCGCG GCGCGGCTTC GGTCCCAAAC   121 CGAAAGCAAA AATTCAGACG GAGGCGGGAA CTGTTTTAGG TTCTCGTCTC CTACCAGAAC   181 CACATATCCC TCCTCTAAGG GGGGTGCACC AAAGAGTCCA AAACGATCGG GATTTTTGGA   241 CTCAGGTCGG GCCACAAAAA CGGCCCCCGA AGTCCCTGGG ACGTCTCCCA GGGTTGCGGC   301 CGGGTGTTCC GAACTCGTCA GTTCCACCAC GGGTCCGCCA GATACAGAGC TAGTTAGCTA   361 ACTAGTACCG ACGCAGGCGC ATAAAATCAG TCATAGACAC TAGACAATCG GACAGACACA   421 GATAAGTTGC TGGCCAGCTT ACCTCCCGGT GGTGGGTCGG TGGTCCCTGG GCAGGGGTCT   481 CCCGATCCCG GACGAGCCCC CAAATGAAAG ACCCCCGCTG ACGGGTAGTC AATCACTCAG   541 AGGAGACCCT CCCNAGGAAC AGCGAGACCA CAAGTCGGAT GCAACTGCAA GAGGGTTTAT   601 TGGATACACG GGTANCCGGG CGANTCNGTC AATCGNANGA NTGGCGCGCC NANTGAGGGG   661 NTGTGGGCTC NTTTATTGAN NTCGGGGANC ANAANCGCGC GANNNGAANC NANNNCGAAN 721 NGANNNNNNN NNN //
Primer: pMFG_1 (Pr0230) Region: pol region,gag Sequence file: RDB19179_B1Ftf.seq
>14601_19179_B1Ft_2_pMFG_1_D03_12_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNTCTNGT NNTGTCAGGG TCAGGGNAGT TTACAAGGGG    61 CGGGGGTGCC CCATATAAGA TCTCATATGG CCATATGGGG GCGCCTAGAG AAGGAGTGAG   121 GGCTGGATAA AGGGAGGATC GAGGCGGGGT CGAACGAGGA GGTTCAAGGG GGAGAGACGG   181 GGCGGATGGA GGAAGAGGAG GCGGAGGCTT AGGGTGTACA AAGGGCTTGA CCCAGGGAGG   241 GGGGTCAAAA GCCAAGGCTT CCCAGGTCAC GATGTAGGGG ACCTGGTCTG GGTGTCCATG   301 CGGGCCAGGT GAAAAGACCT TGATCTTAAC CTGGGTGATG AGGTCTCGGT TAAAGGTGCC   361 GTCTCGCGGC CATCCGACGT TAAAGGTTGG CCATTCTGCA GAGCAGAAGG TAACCCAACG   421 TCTCTTCTTG ACATCTACCG ACTGGTTGTG AGCGATCCGC TCGACATCTT TCCAGTGACC   481 TAAGGTCAAA CTTAAGGGAG TGGTAACAGT CTGGCCCGGG CCCATATTTT CAGACAAATA   541 CAGAAACACA GTCAGACAGA GACNACACAG AACGATGCTG CAGCAGACAA GACGCGCGGC   601 GCGGCTTCNG TCCCAAACCG AAAGCAAAAA TTCNGANGGA GGCGGGAACT GNTTTAGNNT   661 CTCGTCTCCT ACCAGANNCA CATATCCCTN CNTCTNANNG GGGGNGNANN NNNNNANTNN 721 NNAANNNNTN NNNNNNNTTN TNNNNNNNNN N //
Primer: mCherry-C_R (Pr0517) Region: insert mid(mRFP1) Sequence file: RDB19179_B1Ftg.seq
>14601_19179_B1Ft_2_mCherry-C_R_C03_09_ABI24.ab1     1 NNNNNNNNNN NNNCNNNNNG TCGGTCTTGT AGGCGCCGGG CAGCTGCACG GGCTTCTTGG    61 CCATGTAGGT GGTCTTGACC TCGGCGTCGT AGTGGCCGCC GTCCTTCAGC TTCAGCCTCA   121 TCTTGATCTC GCCCTTCAGG GCGCCGTCCT CGGGGTACAT CCGCTCGGTG GAGGCCTCCC   181 AGCCCATGGT CTTCTTCTGC ATTACGGGGC CGTCGGAGGG GAAGTTGGTG CCGCGCAGCT   241 TCACCTTGTA GATGAACTCG CCGTCCTGCA GGGAGGAGTC CTGGGTCACG GTCACCACGC   301 CGCCGTCCTC GAAGTTCATC ACGCGCTCCC ACTTGAAGCC CTCGGGGAAG GACAGCTTCA   361 AGTAGTCGGG GATGTCGGCG GGGTGCTTCA CGTAGGCCTT GGAGCCGTAC TGGAACTGAG   421 GGGACAGGAT GTCCCAGGCG AAGGGCAGGG GGCCGCCCTT GGTCACCTTC AGCTTGGCGG   481 TCTGGGTGCC CTCGTAGGGG CGGCCCTCGC CCTCGCCCTC GATCTCGAAC TCGTGGCCGT   541 TCACGGAGCC CTCCATGCGC ACCTTGAAGC GCATGAACTC CTTGATGACG TCCTCGGANG   601 ANGNCATCCC GAACGTCTCC TGGGANGCAT AGACCATGTA CNGGAAGCCG TCTTCNTCTC   661 TCTCGCTCTC NNANNCTTNN NANNNGGGNG NGGNNNNNCT CNCNANGCTG NGNCNNTTCN 721 NNNNGNANNA ANANNNNNNN NNN //
Primer: IRES-F (Pr0036) Region: IRES2,PuroR Sequence file: RDB19179_B1Fth.seq
>14601_19179_B1Ft_2_IRES-F_F03_18_ABI24.ab1     1 NNNNNNNNNN NNNNNTNNNN CNNNGTTANN AAACGTCTAG GCCCCCCGAA CCACGGGGNC    61 GTGGTTTTCC TTTGAAAAAC ACGATGATAA TATGGCCACA ACCATGACCG AGTACAAGCC   121 CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCCGGGCC GTACGCACCC TCGCCGCCGC   181 GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGACCCG GACCGCCACA TCGAGCGGGT   241 CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC GACATCGGCA AGGTGTGGGT   301 CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG GAGAGCGTCG AAGCGGGGGC   361 GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC GGTTCCCGGC TGGCCGCGCA   421 GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG GAGCCCGCGT GGTTCCTGGC   481 CACCGTCGGC GTCTCGCCCG ACCACCAGGG CAAGGGTCTG GGCAGCGCCG TCGTGCTCCC   541 CGGAGTGNNN GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC CTGGAGACCT CCGCGCCCCG   601 CAACCNTCCC CCTTCTANNN NNNGCTCGNN NTCACCNGNN NNNGCCNACG TCNAGGTGCC   661 CGNANNNANN NNGCACNNGN NNNNNNGNNC CNNNNNNNCG GNGNCNNNNG NCCNGNNCNN 721 NNNNNNNN //
Primer: p15Aori_F (Pr0276) Region: 3' LTR Sequence file: RDB19179_B1Fti.seq
>14601_19179_B1Ft_2_p15Aori_F_G03_21_ABI24.ab1     1 NNNNNNNNNN NNNNNGNNGC TCNTCNNGGG GGCGGNNCCT ATGGNAAAAC GCCAGCAACG    61 CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT   121 TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC   181 GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC   241 GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAACTATGGC CATTTAATGT   301 AAATACTTAA GAAAAAAAAC CAAATTAATT TTGATACATG CTGCATGTGA AAGACCCCCG   361 CTGACGGGTA GTCAATCACT CAGAGGAGAC CCTCCCAAGG AACAGCGAGA CCACAAGTCG   421 GATGCAACTG CAAGAGGGTT TATTGGATAC ACGGGTACCC GGGCGACTCA GTCAATCGGA   481 GGACTGGCGC CCCGAGTGAG GGGTTGTGGG CTCTTTTATT GAGCTCGGGG AGCAGAAGCG   541 CGCGAACAGA AGCGAGAAGC GAACTGATTG GTTAGTTCAA ATAAGGCACA GGGTCATTTC   601 AGGTCCTTGG GGGCACNCTG GAAACATCTG ATGGNTCTCT AGAAACTGCT GANGGCTGGA   661 CCGCATCTGG GGANCATCNG NTCTTGGNCN TNNNNCNNGG GNNANNNNNG CNTNANNNCN 721 NNNATNCNNG NNTNNGNNNC NNNNNTNNNN NNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.22

GNP_filter3_RDBDEP_html_230512.pl