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p3xFLAG-CMV10-hFIP200

Transient expression vector of human FIP200 with N-terminal 3×FLAG.

Catalog number RDB19762
Resource name p3xFLAG-CMV10-hFIP200
Clone info. Transient expression vector of human FIP200 with N-terminal 3×FLAG.
Vector backbone p3xFLAG-CMV-10 (plasmid)
Size of vector backbone 6.3 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Gene/insert name human RB1CC1 cDNA; synthetic 3×FLAG-tag.
Depositor|Developer Mizushima, Noboru |
 
Sequence (full) RDB19762hts01.seqcheck assembled from experimentally sequenced data
Test sheet RDB19762_B2Jlp1-1.pdf 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Hara, T. et al. J. Cell Biol. 181(3): 497-510, 2008)
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提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Hara, T. et al. J. Cell Biol. 181(3): 497-510, 2008)。
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19762 p3xFLAG-CMV10-hFIP200 DNA solution


How to cite this biological resource

Materials & Methods section:

The p3xFLAG-CMV10-hFIP200 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19762).

Reference section:

Hara, T., Takamura, A., Kishi, C., Iemura, S., Natsume, T., Guan, J.L., Mizushima, N., FIP200, a ULK-interacting protein, is required for autophagosome formation in mammalian cells. J. Cell Biol. 181 (3): 497-510 (2008). PMID 18443221. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles

original Hara, T., FIP200, a ULK-interacting protein, is required for autophagosome formation in mammalian cells. J. Cell Biol. 181 (3): 497-510 (2008). PMID 18443221. [link to RRC of NBRP]