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pMRXIP-VPS16-GFP

Retroviral vector for stable expression of human VPS16 with C-terminal EGFP.

Catalog number RDB19787
Resource name pMRXIP-VPS16-GFP
Clone info. Retroviral vector for stable expression of human VPS16 with C-terminal EGFP.
Vector backbone pMRXIP GFP (plasmid)
Selectable markers Ampicillin (E. coli), Puromycin (mammalian cell)
Gene/insert name human VPS16 cDNA; Aequorea victoria GFP (EGFP).
Depositor|Developer Mizushima, Noboru |
Other clones in our bank
External Database
human VPS16
          Reference sequence
            
           
          Sequence (full) RDB19787hts01.seqcheck assembled from experimentally sequenced data
          Test sheet RDB19787_B2Jlp1-1.pdf 

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Jiang, P. et al. Mol. Biol. Cell. 25(8): 1327-1337, 2014)
          Additional terms and conditions:
          Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Please visit Information of Request for Distribution.[link] 
          Remarks Remember that you will be working with samples containing infectious virus.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Jiang, P. et al. Mol. Biol. Cell. 25(8): 1327-1337, 2014)。
          付加的提供条件：
          pMRXベクターについて：利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
          提供依頼
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          手続きの概要は、「提供申込みについて[link]」をご覧ください。
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB19787 pMRXIP-VPS16-GFP DNA solution

          How to cite this biological resource

          Materials & Methods section:

          The pMRXIP-VPS16-GFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19787).

          Reference section:

          Jiang, P., Nishimura, T., Sakamaki, Y., Itakura, E., Hatta, T., Natsume, T., Mizushima, N., The HOPS complex mediates autophagosome-lysosome fusion through interaction with syntaxin 17. Mol. Biol. Cell 25 (8): 1327-1337 (2014). PMID 24554770. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

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          References

          Original, user report and related articles

          original Jiang, P., The HOPS complex mediates autophagosome-lysosome fusion through interaction with syntaxin 17. Mol. Biol. Cell 25 (8): 1327-1337 (2014). PMID 24554770. [link to RRC of NBRP]

          2023.08.29

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