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pMXsIP-GFP-VPS33A

Retroviral vector for stable expression of human VPS33A with N-terminal GFP.

Catalog number RDB19783
Resource name pMXsIP-GFP-VPS33A
Clone info. Retroviral vector for stable expression of human VPS33A with N-terminal GFP.
Vector backbone pMXs-IP (plasmid)
Selectable markers Ampicillin (E. coli), Puromycin (mammalian cell)
Gene/insert name human VPS33A cDNA; Aequorea victoria GFP (EGFP).
Depositor|Developer Mizushima, Noboru |
 
Sequence (full) RDB19783hts01.seqcheck assembled from experimentally sequenced data
Test sheet RDB19783_B2Jlp1-1.pdf 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Jiang, P. et al. Mol. Biol. Cell. 25(8): 1327-1337, 2014)
Additional terms and conditions:
Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
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MTA, for use for not-for-profit academic purpose [Word]
Please visit Information of Request for Distribution.[link] 
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Jiang, P. et al. Mol. Biol. Cell. 25(8): 1327-1337, 2014)。
付加的提供条件:
pMXsシリーズベクターについて:利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所 北村俊雄博士への謝辞の表明を必要とする。
提供依頼
提供依頼書 [Word]
提供同意書 (MTA, 非営利学術目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19783 pMXsIP-GFP-VPS33A DNA solution


How to cite this biological resource

Materials & Methods section:

The pMXsIP-GFP-VPS33A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19783).

Reference section:

Jiang, P., Nishimura, T., Sakamaki, Y., Itakura, E., Hatta, T., Natsume, T., Mizushima, N., The HOPS complex mediates autophagosome-lysosome fusion through interaction with syntaxin 17. Mol. Biol. Cell 25 (8): 1327-1337 (2014). PMID 24554770. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles

original Jiang, P., The HOPS complex mediates autophagosome-lysosome fusion through interaction with syntaxin 17. Mol. Biol. Cell 25 (8): 1327-1337 (2014). PMID 24554770. [link to RRC of NBRP]