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p3xFLAG-CMV10-mApg16L (#RDB20243)

Transient expression vector of mouse Atg16l1 with N-terminal 3×FLAG.


Drawn by SnapGene® software
Sequence (full) RDB20243hts01.seq
Publication Mizushima, N., J. Cell Sci. 116 (Pt 9): 1679-1688 (2023). [link to RRC of NBRP]
Test sheet RDB20243_B4App1-1.pdf 
Assembled from experimentally sequenced data.
 
Clone info. Transient expression vector of mouse Atg16l1 with N-terminal 3×FLAG.
Comment There is a mutation from A to G substitution at 923nt (D101G) in the NeoR gene. However, Neomycin resistance in mammalian cells was confirmed by the depositor.
Vector backbone p3xFLAG-CMV-10 (plasmid)
Size of vector backbone 6.3 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Gene/insert name mouse ATG16L1 cDNA; synthetic 3×FLAG-tag.
Depositor|Developer Mizushima, Noboru |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Mizushima, N. et al. J. Cell Sci. 116(Pt 9): 1679-1688, 2003)
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Mizushima, N. et al. J. Cell Sci. 116(Pt 9): 1679-1688, 2003)。

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RDB20243 p3xFLAG-CMV10-mApg16L DNA solution

How to cite this biological resource

Materials & Methods section:

The p3xFLAG-CMV10-mApg16L was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20243).

Reference section:

Mizushima, N., Kuma, A., Kobayashi, Y., Yamamoto, A., Matsubae, M., Takao, T., Natsume, T., Ohsumi, Y., Yoshimori, T., Mouse Apg16L, a novel WD-repeat protein, targets to the autophagic isolation membrane with the Apg12-Apg5 conjugate. J. Cell Sci. 116 (Pt 9): 1679-1688 (2023). PMID 12665549. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Mizushima, N., Mouse Apg16L, a novel WD-repeat protein, targets to the autophagic isolation membrane with the Apg12-Apg5 conjugate. J. Cell Sci. 116 (Pt 9): 1679-1688 (2023). PMID 12665549. [link to RRC of NBRP]