Resource data sheet
DNA Bank Top

p3xFLAG-CMV10-hTel2 (#RDB19795)

Transient expression vector of human Tel2 with N-terminal 3×FLAG.


Drawn by SnapGene® software
Sequence (full) RDB19795hts01.seq
Publication Kaizuka, T., J. Biol. Chem. 285 (26): 20109-20116 (2010). [link to RRC of NBRP]
Test sheet RDB19795_B2Jlp1-1.pdf 
Assembled from experimentally sequenced data.
 
Clone info. Transient expression vector of human Tel2 with N-terminal 3×FLAG.
Vector backbone p3xFLAG-CMV-10 (plasmid)
Size of vector backbone 6.3 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Gene/insert name human TELO2 cDNA; synthetic 3×FLAG-tag.
Depositor|Developer Mizushima, Noboru |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kaizuka, T. et al. J. Biol. Chem. 285(26): 20109-20116, 2010)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kaizuka, T. et al. J. Biol. Chem. 285(26): 20109-20116, 2010)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19795 p3xFLAG-CMV10-hTel2 DNA solution


How to cite this biological resource

Materials & Methods section:

The p3xFLAG-CMV10-hTel2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19795).

Reference section:

Kaizuka, T., Hara, T., Oshiro, N., Kikkawa, U., Yonezawa, K., Takehana, K., Iemura, S., Natsume, T., Mizushima, N., Tti1 and Tel2 are critical factors in mammalian target of rapamycin complex assembly. J. Biol. Chem. 285 (26): 20109-20116 (2010). PMID 20427287. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles

original Kaizuka, T., Tti1 and Tel2 are critical factors in mammalian target of rapamycin complex assembly. J. Biol. Chem. 285 (26): 20109-20116 (2010). PMID 20427287. [link to RRC of NBRP]