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pCI-neo-hApg5(K130R)-HA

Expression vector of human Apg5 mutant (K130R) with C-terminal HA.

Catalog number RDB19799
Resource name pCI-neo-hApg5(K130R)-HA
Clone info. Expression vector of human Apg5 mutant (K130R) with C-terminal HA.
Vector backbone pCI-neo (plasmid)
Size of vector backbone 5.5 kb
Selectable markers Ampicillin
Gene/insert name human ATG5 cDNA; synthetic HA-tag.
Depositor|Developer Mizushima, Noboru |
 
Sequence (full) RDB19799hts01.seqcheck assembled from experimentally sequenced data
Test sheet RDB19799_B2Jlp1-1.pdf 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Mizushima, N. et al. J. Biol. Chem. 273(51): 33889-33892, 1998)
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提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Mizushima, N. et al. J. Biol. Chem. 273(51): 33889-33892, 1998)。
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19799 pCI-neo-hApg5(K130R)-HA DNA solution


How to cite this biological resource

Materials & Methods section:

The pCI-neo-hApg5(K130R)-HA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19799).

Reference section:

Mizushima, N., Sugita, H., Yoshimori, T., Ohsumi, Y., A new protein conjugation system in human. The counterpart of the yeast Apg12p conjugation system essential for autophagy. J. Biol. Chem. 273 (51): 33889-33892 (1998). PMID 9852036. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles

original Mizushima, N., A new protein conjugation system in human. The counterpart of the yeast Apg12p conjugation system essential for autophagy. J. Biol. Chem. 273 (51): 33889-33892 (1998). PMID 9852036. [link to RRC of NBRP]