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pCW57-CMV-ssRFP-GFP-KDEL (#RDB19767)

Lentiviral vector for inducible expression of ER-phagy marker ssRFP-GFP-KDEL.


Drawn by SnapGene® software
Sequence (full) RDB19767hts01.seq
Publication Chino, H., Mol. Cell 74 (5): 909-921.e6 (2019). [link to RRC of NBRP]
Test sheet RDB19767_B2Jlp1-1.pdf 
Assembled from experimentally sequenced data.
 
Clone info. Lentiviral vector for inducible expression of ER-phagy marker ssRFP-GFP-KDEL. See Fig.4 of Chino, H. et al., Mol. Cell, 74 (5): 909-921, 2019.
Vector backbone pCW57-CMV (plasmid)
Selectable markers Ampicillin (E. coli), Puromycin (mammalian cell)
Growth conditions LB+Amp, 37oC
Gene/insert name Aequorea victoria GFP (EGFP); Discosoma sp. RFP (ssRFP).
Depositor|Developer Mizushima, Noboru |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Chino, H. et al. Mol. Cell. 74(5): 909-921.e6, 2019)
Remarks Remember that you will be working with samples containing infectious virus.
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Chino, H. et al. Mol. Cell. 74(5): 909-921.e6, 2019)。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19767 pCW57-CMV-ssRFP-GFP-KDEL DNA solution

How to cite this biological resource

Materials & Methods section:

The pCW57-CMV-ssRFP-GFP-KDEL was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19767).

Reference section:

Chino, H., Hatta, T., Natsume, T., Mizushima, N., Intrinsically Disordered Protein TEX264 Mediates ER-phagy. Mol. Cell 74 (5): 909-921.e6 (2019). PMID 31006538. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Chino, H., Intrinsically Disordered Protein TEX264 Mediates ER-phagy. Mol. Cell 74 (5): 909-921.e6 (2019). PMID 31006538. [link to RRC of NBRP]