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pMXs-puro HA-WIPI-1

Retroviral vector for stable expression of human WIPI-1 with N-terminal HA.

Catalog number RDB19788
Resource name pMXs-puro HA-WIPI-1
Clone info. Retroviral vector for stable expression of human WIPI-1 with N-terminal HA.
Vector backbone pMXs-puro (plasmid)
Selectable markers Ampicillin (E. coli), Puromycin (mammalian cell)
Gene/insert name human WIPI1 cDNA; synthetic HA-tag.
Depositor|Developer Mizushima, Noboru |
Other clones in our bank
External Database
human WIPI1
          Reference sequence
            
           
          Sequence (full) RDB19788hts01.seqcheck assembled from experimentally sequenced data
          Test sheet RDB19788_B2Jlp1-1.pdf 

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Itakura, E. Mizushima, N. Autophagy. 6(6): 764-776, 2010)
          Additional terms and conditions:
          Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Please visit Information of Request for Distribution.[link] 
          Remarks Remember that you will be working with samples containing infectious virus.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Itakura, E. Mizushima, N. Autophagy. 6(6): 764-776, 2010)。
          付加的提供条件：
          pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
          提供依頼
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          手続きの概要は、「提供申込みについて[link]」をご覧ください。
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB19788 pMXs-puro HA-WIPI-1 DNA solution

          How to cite this biological resource

          Materials & Methods section:

          The pMXs-puro HA-WIPI-1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19788).

          Reference section:

          Itakura, E., Mizushima, N., Characterization of autophagosome formation site by a hierarchical analysis of mammalian Atg proteins. Autophagy 6 (6): 764-776 (2010). PMID 20639694. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

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          References

          Original, user report and related articles

          original Itakura, E., Characterization of autophagosome formation site by a hierarchical analysis of mammalian Atg proteins. Autophagy 6 (6): 764-776 (2010). PMID 20639694. [link to RRC of NBRP]

          2023.08.29

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