Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMXs-IP-EGFP-hATG13

Retroviral vector for stable expression of human ATG13 with N-terminal EGFP.

Catalog number RDB19625
Resource name pMXs-IP-EGFP-hATG13
Clone info. Retroviral vector for stable expression of human ATG13 with N-terminal EGFP.
Vector backbone pMXs-IP (plasmid)
Selectable markers Ampicillin (E. coli), Puromycin (mammalian cell)
Gene/insert name human ATG13; Aequorea victoria GFP.
Depositor|Developer Mizushima, Noboru |
Other clones in our bank

External Database
human ATG13

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Hosokawa, N. et al. Mol. Biol. Cell, 20(7), 1981-1991, 2009)
          Additional terms and conditions:
          Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks Remember that you will be working with samples containing infectious virus.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hosokawa, N. et al. Mol. Biol. Cell, 20(7), 1981-1991, 2009)。
          付加的提供条件:
          pMXsシリーズベクターについて:利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所 北村俊雄博士への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB19625 pMXs-IP-EGFP-hATG13 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pMXs-IP-EGFP-hATG13 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19625).

          Reference section:

          Hosokawa, N., Hara, T., Kaizuka, T., Kishi, C., Takamura, A., Miura, Y., Iemura, S., Natsume, T., Takehana, K., Yamada, N., Guan, J.L., Oshiro, N., Mizushima, N., Nutrient-dependent mTORC1 association with the ULK1-Atg13-FIP200 complex required for autophagy. Mol. Biol. Cell 20 (7): 1981-1991 (2009). PMID 19211835.

          Further references such as user reports and related articles (go to bottom)


          References and tips

          Featured content


          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB19829_B2Lgp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: MMLV-pol_F (Pr0352)
          Region: pol region,insert 5'(EGFP 5')
          Sequence file: RDB19829_B2Lga.seq check
          >D08693C3_B2Lg_3_MMLV-pol-F_A01_01_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NGNNTNNNNN CTTGGNNACN CGCCGCCCNC GTGAAGGCTG
             61 CCGACCCCGG GGGTGGACCA TCCTCTAGAC TGCCGGATCT AGCTAGTTAA TTAAGGATCC
            121 CAGTGTGGTG GTACGGGAAT TCCTGCAGGC CTCGAGGGCC GGCGCGCCGC GGCCGCCGCC
            181 ACCATGGTGA GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG
            241 GACGGCGACG TAAACGGCCA CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC
            301 TACGGCAAGC TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC
            361 ACCCTCGTGA CCACCCTGAC CTACGGCGTG CAGTGCTTCA GCCGCTACCC CGACCACATG
            421 AAGCAGCACG ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC
            481 TTCTTCAAGG ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC
            541 CTGGTGAACC GCATCGAGCT GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG
            601 CACAAGCTGG AGTACAACTA CAACAGCCAC AACGTCTATA TCATGGCCGA CAAGCAGAAG
            661 AACGGNNTCA AGGTGAACTT CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC
            721 GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACNAC
            781 CACTACCTGA GCACCCAGTC CGCCCTGAGC AAAGACCCCN NCGAGAAGCG CGATCACATG
            841 GTCCTGCTGG ANNNCNNNAC CGCCGCCGGG ATCANTCTCN GNCATGGACN ANCTGTACAA
            901 GTCCNGGANT CNGATCTCNA GCTCAAGCTT CNNATTCTNN NNNNCNACGG TNNCGCGGGC
            961 CNNGNNNNCN NGGNANNTGA TNNNNATTNC NANGNNANAA NNNANCTNNN NNNNNTNATN
           1021 NNNTTTTTGC CNNNANANTG NCCNNNNGAN NGTCNNNGNN NNGCTNNNNA AANNNTTNNN
          1081 NTCNNNCATN ANNNNNNNNN N
          //
          Primer: hATG13-C_F (Pr1278)
          Region: insert 3'(hATG13 3'),IRES
          Sequence file: RDB19829_B2Lgb.seq check
          >D08692C3_B2Lg_3_hATG13-C_F_C01_03_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNGTTTGC TCCNNNGAAT TTGGAGCTGG AGGANACCGA
             61 TCCAATGGTG AATCCTCCNN ANTCCCCAGA GACTGAATCT CCTCTCCAGG GCAGCCTGCA
            121 CTCAGATGGC TCCAGCGGGG GCAGCAGTGG CAATACCCAT GATGACTTTG TTATGATAGA
            181 CTTTAAACCA GCTTTTTCTA AAGATGACAT TCTTCCGATG GACCTGGGGA CCTTCTATCG
            241 GGAGTTTCAG AACCCACCTC AGCTGAGCAG CCTCTCCATA GATATTGGAG CACAGTCCAT
            301 GGCTGAAGAC TTGGACTCAT TACCAGAGAA GCTGGCTGTG CATGAGAAGA ATGTCCGCGA
            361 GTTTGATGCC TTTGTGGAAA CCCTGCAGGG ATCCACCGGA TCTAGATAAC TGATCATAAT
            421 CAGCCATACC ACATTTGTAG GCGGCCGCTA CGTAAATTCC GCCCCCCCCC CCCCTCTCCN
          //
          Primer: FP-N_F1 (Pr0920)
          Region: insert mid(EGFP 3',hATG13 5')
          Sequence file: RDB19829_B2Lgc.seq check
          >D08693C3_B2Lg_3_FP-N_F1_C01_03_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNCNNNN NNNNNNGAAG CAGCACGACT TCTTCNAGTC
             61 CGCCNTGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA
            121 CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA
            181 GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA
            241 CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG TGAACTTCAA
            301 GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC
            361 CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGTCCGC
            421 CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC
            481 CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGTCC GGACTCAGAT CTCGAGCTCA
            541 AGCTTCGAAT TCTGCAGTCG ACGGTACCGC GGGCCCGGGA TCCATGGAAA CTGATCTCAA
            601 TTCCCAGGAC AGAAAGGACC TGGACAAGTT TATTAAATTT TTTGCCCTCA AGACTGTCCA
            661 AGTGATTGTC CAGGCTCGGC TTGGTGAAAA GATTTGCACT CGTTCATCAT CTTCTCCAAC
            721 GGGTTCAGAT TGGTTCAACT TAGCAATCAA AGACATCCCA GAGGTTACAC ATGAAGCAAA
            781 GAAGGCNCTG GCAGGACAGC TGCCTGCAGT CGGGAGGNCC NNTGTGTGTG GANNTTTCAC
            841 TTANGACTTC TGANGGAGNN NNCNNTGGAN NTGGAAATAT GGTGTCTTGA AATGAATGAA
            901 AANTGTGATN AAGAAATCAA AGTTTCCNAN NCNGTGTACA ACANANNGTC NNTGCTGCTG
            961 AANNNCNNNC TTGCTATANC TAGGGNGACA CCNNNCNNNN NNNNTCTCCA NNAAACNNGG
           1021 NCNTGNATAT GTNNTATTNN ANNNGNNNNN NNNTTNGGNN NNANTTCNNC TGANNGNCNT
          1081 NNNNANNNNN NTNCNNNNNN TNNNNNNNNG GGNNNNNNNN NN
          //
          Primer: EGFP-C (Pr0025)
          Region: insert mid(hATG13 5')
          Sequence file: RDB19829_B2Lgd.seq check
          >D08693C3_B2Lg_3_EGFP-C_D01_04_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNAN NNTNNNNNNN NNCCGGANTC NGATCTCGAG
             61 CTCAAGCTTC GAATTCTGCA GTCGACGGTA CCGCGGGCCC GGGATCCATG GAAACTGATC
            121 TCAATTCCCA GGACAGAAAG GACCTGGACA AGTTTATTAA ATTTTTTGCC CTCAAGACTG
            181 TCCAAGTGAT TGTCCAGGCT CGGCTTGGTG AAAAGATTTG CACTCGTTCA TCATCTTCTC
            241 CAACGGGTTC AGATTGGTTC AACTTAGCAA TCAAAGACAT CCCAGAGGTT ACACATGAAG
            301 CAAAGAAGGC ACTGGCAGGA CAGCTGCCTG CAGTCGGGAG GTCCATGTGT GTGGAGATTT
            361 CACTTAAGAC TTCTGAGGGA GATTCCATGG AGCTGGAAAT ATGGTGTCTT GAAATGAATG
            421 AAAAGTGTGA TAAAGAAATC AAAGTTTCCT ACACGGTGTA CAACAGACTG TCATTGCTGC
            481 TGAAGTCCCT TCTTGCTATA ACTAGGGTGA CACCAGCCTA TAGGCTCTCC AGGAAACAAG
            541 GGCATGAATA TGTCATATTA TACAGGATAT ATTTTGGAGA AGTTCAGCTG AGTGGCTTAG
            601 GAGAAGGCTT CCAGACAGTT CGTGTTGGGA CAGTGGGCAC CCCTGTGGGC ACCATCACTC
            661 TTTCTTGTGC TTANNNAATT AACTTGGCAT TCATGTCTAC CAGGCAATTT GAGAGGACCC
            721 CACCTATCAT GGGGATTATT ATTGATCACT TTGTGGACCG TCCCTATCCC AGCTCCTCTC
            781 CCATGCACCC CTGCAATTAC NGAACTGCTG GNGAGGACAC TGGANTAATA TACCCGTCTG
            841 TAGAAGANTC TCAAGAAGTG TGNNNCNNCT CTTTTNNCAC CTCCCCACNN NCNCAGCNCT
            901 CAAGCTCTCN CNTTTNCCNA TNNNCNNGCT NCCCTGGGCG NNGNNNNCAN CNGNNCNNGN
            961 NTTANCCNGN ANNGNTNNCN NNNNGNCTNN AATGNNNNNN ACCCTCNCCA NCTGNNGGNT
           1021 TNCNNNNNAN NNANNNNGGG GNNNNNNCTN GNNCNNACNA NNCNNGNNCA NNGNACCNNN
          1081 NNNNNNN
          //
          Primer: MMLV psi_R1 (Pr1054)
          Region: 5' LTR
          Sequence file: RDB19829_B2Lge.seq check
          >D08693C3_B2Lg_3_MMLV psi_R1_E01_05_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNGTTGCT GGCCNGCNNN
             61 NNNCCCGNNT GGTGGGTCGG TGGNCCNNGN NNNNGGGTCT CCCGATCCCG GACGAGCCCC
            121 CAAATGAAAG ACCCCCGCTG ACGGGTAGTC AATCACTCAN AGGAGACCCT CCCAAGGAAC
            181 AGCGAGACCA CAAGTCGGAT GCAACTGCAA GAGGGTTTAT TGGATACACG GGTACCCGGG
            241 CGACTCAGTC AATCGGAGGA CTGGCGCGCC GAGTGAGGGG TTGTGGGCTC TTTTATTGAG
            301 CTCGGGGAGC AGAAGCGCGC GAACAGAAGC GAGAAGCGAA CTGATTGGTT AGTTCAAATA
            361 AGGCACAGGG TCATTTCAGG TCCTTGGGGC ACCCTGGAAA CATCTGATGG TTCTCTAGAA
            421 ACTGCTGAGG GCTGGACCGC ATCTGGGGAC CATCTGTTCT TGGCCCTGAG CCGGGGCAGG
            481 AACTGCTTAC CACAGATATC CTGTTTGGCC CATATTCNNC TGTNNNNNNT GTTCTTGGCC
            541 CTGAGCCGGG GCAGGAACTG CTTACCACAG ANNNNNTGTT TGGCCCATAT TNNNNNNTTC
            601 CATCTGTTCC TGACCTTGAT CTGAACTTTT CTATTCTCAG TTNTGTATTT TTCCATGCNN
            661 NNNNNNATGG CGTTACTTAN NCTAGCTTGC CNAACCTANN NGTGGGGTCT TTCATTATNC
          721 NNGTGGCACT TTTCNNNNAA ATGTGCGCGG AACCCCTATT TNNNNNNNNN N
          //
          Primer: MMLV psi_F (Pr0863)
          Region: MMLV psi,gag,pol region
          Sequence file: RDB19829_B2Lgf.seq check
          >D08693C3_B2Lg_3_MMLV psi_F_F01_06_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNTNN NNNNNNNNTT TCGGTTTGGG ANCGAAGCCG
             61 CGCCGCGCGT CTTGTCNNCT GNNNCATCGT TCTGTGTTGT CTCTGTCTGA CTGTGTTTCT
            121 GTATTTGTCT GAAAATTAGG GCCAGACTGT TACCACTCCC TTAAGTTTGA CCTTAGGTCA
            181 CTGGAAAGAT GTCGAGCGGA TCGCTCACAA CCAGTCGGTA GATGTCAAGA AGAGACGTTG
            241 GGTTACCTTC TGCTCTGCAG AATGGCCAAC CTTTAACGTC GGATGGCCGC GAGACGGCAC
            301 CTTTAACCGA GACCTCATCA CCCAGGTTAA GATCAAGGTC TTTTCACCTG GCCCGCATGG
            361 ACACCCAGAC CAGGTCCCCT ACATCGTGAC CTGGGAAGCC TTGGCTTTTG ACCCCCCTCC
            421 CTGGGTCAAG CCCTTTGTAC ACCCTAAGCC TCCGCCTCCT CTTCCTCCAT CCGCCCCGTC
            481 TCTCCCCCTT GAACCTCCTC GTTCGACCCC GCCTCGATCC TCCCTTTATC CAGCCCTCAC
            541 TCCTTCTCTA GGCGCCCCCA TATGGCCATA TGAGATCTTA TATGGGGCAC CCCCGCCCCT
            601 TGTAAACTTC CCTGACCCTG ACATGACNNN ANTTACTAAC AGCCCCTCTC TCCAAGCTCA
            661 CTTNNNNGCT CTCTACTTAG TCCANCNCGA AGTCTGGAGA NNNNNGGCGG CAGCCTACCA
            721 AGAACANNNN NNCCGACCGG NGGNNNCTCA CCCTTANCGA GTCGGCNANN NNNNGTGGGT
            781 CCGCCGANAC CANANNNNNA ANCNNNAACC TCGCTGGAAA GGANCTTACN NNNTCCTGCT
            841 GANCACCCNN NNNNNCCCTC NAANNANANN GNNTCNCANC TNGNNNNCNN NNCNNCCCAC
          901 NTNAANGNTG NCNNNCNNNG NGGNNNNNNN NNN
          //
          Primer: IRES-F (Pr0036)
          Region: IRES,puroR
          Sequence file: RDB19829_B2Lgg.seq check
          >D08693C3_B2Lg_3_IRES-F_A01_01_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNT NNGTNNNNNT NNNNNNNNNN TCTAGGCCCC CCGAACCACG
             61 GGGANGTGGT TTTCCTTTGA AAAACACGAT GATAATATGG CCACAACCAT GGTTACCGAG
            121 TACAAGCCCA CGGTGCGCCT CGCCACCCGC GACGACGTCC CCAGGGCCGT ACGCACCCTC
            181 GCCGCCGCGT TCGCCGACTA CCCCGCCACG CGCCACACCG TCGATCCGGA CCGCCACATC
            241 GAGCGGGTCA CCGAGCTGCA AGAACTCTTC CTCACGCGCG TCGGGCTCGA CATCGGCAAG
            301 GTGTGGGTCG CGGACGACGG CGCCGCGGTG GCGGTCTGGA CCACGCCGGA GAGCGTCGAA
            361 GCGGGGGCGG TGTTCGCCGA GATCGGCCCG CGCATGGCCG AGTTGAGCGG TTCCCGGCTG
            421 GCCGCGCAGC AACAGATGGA AGGCCTCCTG GCGCCGCACC GGCCCAAGGA GCCCGCGTGG
            481 TTCCTGGCCA CCGTCGGCGT CTCGCCCGAC CACCAGGGCA AGGGTCTGGG CAGCGCCGTC
            541 GTGCTCCCCG GAGTGGAGGC GGCCGAGCGC GCCGGGGTGC CCGCCTTCCT GGAGACCTCC
            601 GCGCCCCGCA ACCTCCCCTT CTACGAGCGG CTCGGCTTCA CCGTCACCGC CGACGTCGAG
            661 GTGCCCGAAG GACCGCGCAC CTGGTGCATG ACCCGCAAGC CCGGTGCCTG AGTCGACGAT
            721 AAAATAAAAG ANTTTATTTA GTCTCCNGAA AAAGGGGGGA ATGNAAAGAC CCCACCTGTA
            781 GGTTTGGCAA GCTAGCTNAA NTAACGCCAT TTTGCAAGGC ATGGAAAAAT ACATNACTGA
            841 NAATANAGAA NTTCANATCN NGGTCAGGAA NNNATNGNAC AGCTGAATNT GGGCCNNACA
            901 GGATATCTGT GGTAAGCAGT TNCTGNCCCG GCNNNNGNCC AANAACNGAT GGAACAGCTG
            961 AATATGGNNN NNANNNGANN TCNGTGGTAN NCAGTTNCTN NCCCNGNTNN NGNCCNANNN
           1021 NNNATNGNNN NNNNNANGNN GNCNGNNNNN NNNNANTTTN NNNNNNNAAN NANNNNANNG
          1081 TTNNNNNNGG GNNNNNCCCN NNGNNNNNNN ANNNNNCCNN NNNNNNNN
          //
          Primer: PuroR_F (Pr0554)
          Region: 3' LTR
          Sequence file: RDB19829_B2Lgh.seq check
          >D08693C3_B2Lg_3_PuroR_F_C01_03_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNGNNC GCGCACCTGG TGCNTGACCC
             61 GCAAGCCCGG TGCCTGNNTC NANGATAAAA TAAAAGATTT TATTTAGTCT CCAGAAAAAG
            121 GGGGGAATGA AAGACCCCAC CTGTAGGTTT GGCAAGCTAG CTTAAGTAAC GCCATTTTGC
            181 AAGGCATGGA AAAATACATA ACTGAGAATA GAGAAGTTCA GATCAAGGTC AGGAACAGAT
            241 GGAACAGCTG AATATGGGCC AAACAGGATA TCTGTGGTAA GCAGTTCCTG CCCCGGCTCA
            301 GGGCCAAGAA CAGATGGAAC AGCTGAATAT GGGCCAAACA GGATATCTGT GGTAAGCAGT
            361 TCCTGCCCCG GCTCAGGGCC AAGAACAGAT GGTCCCCAGA TGCGGTCCAN CCCTCAGCAG
            421 TTTCTAGAGA ACCATCAGAT GTTTCCAGGG TGCCCCAAGG ACCTGAAATG ACCCTGTGCC
            481 TTATTTGAAC TAACCAATCA GTTCGCTTCT CGCTTCTGTT CGCGCGCTTC TGCTCCCCGA
            541 GCTCAATAAA AGAGCCCACA ACCCCTCACT CGGGGCGCCA GTCCTCCGAT TGACTGAGTC
            601 GCCCGGGTAC CCGTGTATCC AATAAACCCT CTTGCAGTTG CATCCGACTT GTGGTCTCGC
            661 TGTTCCTTGG GAGGGTCTCC TCTGAGTGAT TGACTACCCG TCNNNGGGGG TCTTTCACAT
            721 GCANNNNGNA TCNAAATTAA TTTGGTTTTT TTTCTTAAGT ATTTACNTTA AATGGCCATA
            781 GTTGCNTTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGGCGCTCTT
            841 CCGCTTCCTC GCTCNCTGAC TCGCTGCNCT CGGTCGTTCN GGCTGCNGNN ANNNNNNNNC
            901 NGCTNNCTCN AANGCGGGTA ATNNNGGTNA TCNNCANNAT CNGGGGATAN NNNANGAAAA
            961 GAACATGTGA NCNAANNGNC NNNNNAANGN NNNNNANCNN TAAAANNGNC NNNNNTNNCT
          1021 NGGCNNTTTT NNNNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Hosokawa, N., Nutrient-dependent mTORC1 association with the ULK1-Atg13-FIP200 complex required for autophagy. Mol. Biol. Cell 20 (7): 1981-1991 (2009). PMID 19211835.

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl