Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMXs-IP-EGFP-hATG13

Retroviral vector for stable expression of human ATG13 with N-terminal EGFP.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Hosokawa, N. et al. Mol. Biol. Cell, 20(7), 1981-1991, 2009) Additional terms and conditions: Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hosokawa, N. et al. Mol. Biol. Cell, 20(7), 1981-1991, 2009)。 付加的提供条件： pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB19829_B2Lgp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352) Region: pol region,insert 5'(EGFP 5') Sequence file: RDB19829_B2Lga.seq
>D08693C3_B2Lg_3_MMLV-pol-F_A01_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NGNNTNNNNN CTTGGNNACN CGCCGCCCNC GTGAAGGCTG    61 CCGACCCCGG GGGTGGACCA TCCTCTAGAC TGCCGGATCT AGCTAGTTAA TTAAGGATCC   121 CAGTGTGGTG GTACGGGAAT TCCTGCAGGC CTCGAGGGCC GGCGCGCCGC GGCCGCCGCC   181 ACCATGGTGA GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG   241 GACGGCGACG TAAACGGCCA CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC   301 TACGGCAAGC TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC   361 ACCCTCGTGA CCACCCTGAC CTACGGCGTG CAGTGCTTCA GCCGCTACCC CGACCACATG   421 AAGCAGCACG ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC   481 TTCTTCAAGG ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC   541 CTGGTGAACC GCATCGAGCT GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG   601 CACAAGCTGG AGTACAACTA CAACAGCCAC AACGTCTATA TCATGGCCGA CAAGCAGAAG   661 AACGGNNTCA AGGTGAACTT CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC   721 GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACNAC   781 CACTACCTGA GCACCCAGTC CGCCCTGAGC AAAGACCCCN NCGAGAAGCG CGATCACATG   841 GTCCTGCTGG ANNNCNNNAC CGCCGCCGGG ATCANTCTCN GNCATGGACN ANCTGTACAA   901 GTCCNGGANT CNGATCTCNA GCTCAAGCTT CNNATTCTNN NNNNCNACGG TNNCGCGGGC   961 CNNGNNNNCN NGGNANNTGA TNNNNATTNC NANGNNANAA NNNANCTNNN NNNNNTNATN  1021 NNNTTTTTGC CNNNANANTG NCCNNNNGAN NGTCNNNGNN NNGCTNNNNA AANNNTTNNN 1081 NTCNNNCATN ANNNNNNNNN N //
Primer: hATG13-C_F (Pr1278) Region: insert 3'(hATG13 3'),IRES Sequence file: RDB19829_B2Lgb.seq
>D08692C3_B2Lg_3_hATG13-C_F_C01_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNGTTTGC TCCNNNGAAT TTGGAGCTGG AGGANACCGA    61 TCCAATGGTG AATCCTCCNN ANTCCCCAGA GACTGAATCT CCTCTCCAGG GCAGCCTGCA   121 CTCAGATGGC TCCAGCGGGG GCAGCAGTGG CAATACCCAT GATGACTTTG TTATGATAGA   181 CTTTAAACCA GCTTTTTCTA AAGATGACAT TCTTCCGATG GACCTGGGGA CCTTCTATCG   241 GGAGTTTCAG AACCCACCTC AGCTGAGCAG CCTCTCCATA GATATTGGAG CACAGTCCAT   301 GGCTGAAGAC TTGGACTCAT TACCAGAGAA GCTGGCTGTG CATGAGAAGA ATGTCCGCGA   361 GTTTGATGCC TTTGTGGAAA CCCTGCAGGG ATCCACCGGA TCTAGATAAC TGATCATAAT   421 CAGCCATACC ACATTTGTAG GCGGCCGCTA CGTAAATTCC GCCCCCCCCC CCCCTCTCCN //
Primer: FP-N_F1 (Pr0920) Region: insert mid(EGFP 3',hATG13 5') Sequence file: RDB19829_B2Lgc.seq
>D08693C3_B2Lg_3_FP-N_F1_C01_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNCNNNN NNNNNNGAAG CAGCACGACT TCTTCNAGTC    61 CGCCNTGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA   121 CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA   181 GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA   241 CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG TGAACTTCAA   301 GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC   361 CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGTCCGC   421 CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC   481 CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGTCC GGACTCAGAT CTCGAGCTCA   541 AGCTTCGAAT TCTGCAGTCG ACGGTACCGC GGGCCCGGGA TCCATGGAAA CTGATCTCAA   601 TTCCCAGGAC AGAAAGGACC TGGACAAGTT TATTAAATTT TTTGCCCTCA AGACTGTCCA   661 AGTGATTGTC CAGGCTCGGC TTGGTGAAAA GATTTGCACT CGTTCATCAT CTTCTCCAAC   721 GGGTTCAGAT TGGTTCAACT TAGCAATCAA AGACATCCCA GAGGTTACAC ATGAAGCAAA   781 GAAGGCNCTG GCAGGACAGC TGCCTGCAGT CGGGAGGNCC NNTGTGTGTG GANNTTTCAC   841 TTANGACTTC TGANGGAGNN NNCNNTGGAN NTGGAAATAT GGTGTCTTGA AATGAATGAA   901 AANTGTGATN AAGAAATCAA AGTTTCCNAN NCNGTGTACA ACANANNGTC NNTGCTGCTG   961 AANNNCNNNC TTGCTATANC TAGGGNGACA CCNNNCNNNN NNNNTCTCCA NNAAACNNGG  1021 NCNTGNATAT GTNNTATTNN ANNNGNNNNN NNNTTNGGNN NNANTTCNNC TGANNGNCNT 1081 NNNNANNNNN NTNCNNNNNN TNNNNNNNNG GGNNNNNNNN NN //
Primer: EGFP-C (Pr0025) Region: insert mid(hATG13 5') Sequence file: RDB19829_B2Lgd.seq
>D08693C3_B2Lg_3_EGFP-C_D01_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNAN NNTNNNNNNN NNCCGGANTC NGATCTCGAG    61 CTCAAGCTTC GAATTCTGCA GTCGACGGTA CCGCGGGCCC GGGATCCATG GAAACTGATC   121 TCAATTCCCA GGACAGAAAG GACCTGGACA AGTTTATTAA ATTTTTTGCC CTCAAGACTG   181 TCCAAGTGAT TGTCCAGGCT CGGCTTGGTG AAAAGATTTG CACTCGTTCA TCATCTTCTC   241 CAACGGGTTC AGATTGGTTC AACTTAGCAA TCAAAGACAT CCCAGAGGTT ACACATGAAG   301 CAAAGAAGGC ACTGGCAGGA CAGCTGCCTG CAGTCGGGAG GTCCATGTGT GTGGAGATTT   361 CACTTAAGAC TTCTGAGGGA GATTCCATGG AGCTGGAAAT ATGGTGTCTT GAAATGAATG   421 AAAAGTGTGA TAAAGAAATC AAAGTTTCCT ACACGGTGTA CAACAGACTG TCATTGCTGC   481 TGAAGTCCCT TCTTGCTATA ACTAGGGTGA CACCAGCCTA TAGGCTCTCC AGGAAACAAG   541 GGCATGAATA TGTCATATTA TACAGGATAT ATTTTGGAGA AGTTCAGCTG AGTGGCTTAG   601 GAGAAGGCTT CCAGACAGTT CGTGTTGGGA CAGTGGGCAC CCCTGTGGGC ACCATCACTC   661 TTTCTTGTGC TTANNNAATT AACTTGGCAT TCATGTCTAC CAGGCAATTT GAGAGGACCC   721 CACCTATCAT GGGGATTATT ATTGATCACT TTGTGGACCG TCCCTATCCC AGCTCCTCTC   781 CCATGCACCC CTGCAATTAC NGAACTGCTG GNGAGGACAC TGGANTAATA TACCCGTCTG   841 TAGAAGANTC TCAAGAAGTG TGNNNCNNCT CTTTTNNCAC CTCCCCACNN NCNCAGCNCT   901 CAAGCTCTCN CNTTTNCCNA TNNNCNNGCT NCCCTGGGCG NNGNNNNCAN CNGNNCNNGN   961 NTTANCCNGN ANNGNTNNCN NNNNGNCTNN AATGNNNNNN ACCCTCNCCA NCTGNNGGNT  1021 TNCNNNNNAN NNANNNNGGG GNNNNNNCTN GNNCNNACNA NNCNNGNNCA NNGNACCNNN 1081 NNNNNNN //
Primer: MMLV psi_R1 (Pr1054) Region: 5' LTR Sequence file: RDB19829_B2Lge.seq
>D08693C3_B2Lg_3_MMLV psi_R1_E01_05_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNGTTGCT GGCCNGCNNN    61 NNNCCCGNNT GGTGGGTCGG TGGNCCNNGN NNNNGGGTCT CCCGATCCCG GACGAGCCCC   121 CAAATGAAAG ACCCCCGCTG ACGGGTAGTC AATCACTCAN AGGAGACCCT CCCAAGGAAC   181 AGCGAGACCA CAAGTCGGAT GCAACTGCAA GAGGGTTTAT TGGATACACG GGTACCCGGG   241 CGACTCAGTC AATCGGAGGA CTGGCGCGCC GAGTGAGGGG TTGTGGGCTC TTTTATTGAG   301 CTCGGGGAGC AGAAGCGCGC GAACAGAAGC GAGAAGCGAA CTGATTGGTT AGTTCAAATA   361 AGGCACAGGG TCATTTCAGG TCCTTGGGGC ACCCTGGAAA CATCTGATGG TTCTCTAGAA   421 ACTGCTGAGG GCTGGACCGC ATCTGGGGAC CATCTGTTCT TGGCCCTGAG CCGGGGCAGG   481 AACTGCTTAC CACAGATATC CTGTTTGGCC CATATTCNNC TGTNNNNNNT GTTCTTGGCC   541 CTGAGCCGGG GCAGGAACTG CTTACCACAG ANNNNNTGTT TGGCCCATAT TNNNNNNTTC   601 CATCTGTTCC TGACCTTGAT CTGAACTTTT CTATTCTCAG TTNTGTATTT TTCCATGCNN   661 NNNNNNATGG CGTTACTTAN NCTAGCTTGC CNAACCTANN NGTGGGGTCT TTCATTATNC 721 NNGTGGCACT TTTCNNNNAA ATGTGCGCGG AACCCCTATT TNNNNNNNNN N //
Primer: MMLV psi_F (Pr0863) Region: MMLV psi,gag,pol region Sequence file: RDB19829_B2Lgf.seq
>D08693C3_B2Lg_3_MMLV psi_F_F01_06_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNTNN NNNNNNNNTT TCGGTTTGGG ANCGAAGCCG    61 CGCCGCGCGT CTTGTCNNCT GNNNCATCGT TCTGTGTTGT CTCTGTCTGA CTGTGTTTCT   121 GTATTTGTCT GAAAATTAGG GCCAGACTGT TACCACTCCC TTAAGTTTGA CCTTAGGTCA   181 CTGGAAAGAT GTCGAGCGGA TCGCTCACAA CCAGTCGGTA GATGTCAAGA AGAGACGTTG   241 GGTTACCTTC TGCTCTGCAG AATGGCCAAC CTTTAACGTC GGATGGCCGC GAGACGGCAC   301 CTTTAACCGA GACCTCATCA CCCAGGTTAA GATCAAGGTC TTTTCACCTG GCCCGCATGG   361 ACACCCAGAC CAGGTCCCCT ACATCGTGAC CTGGGAAGCC TTGGCTTTTG ACCCCCCTCC   421 CTGGGTCAAG CCCTTTGTAC ACCCTAAGCC TCCGCCTCCT CTTCCTCCAT CCGCCCCGTC   481 TCTCCCCCTT GAACCTCCTC GTTCGACCCC GCCTCGATCC TCCCTTTATC CAGCCCTCAC   541 TCCTTCTCTA GGCGCCCCCA TATGGCCATA TGAGATCTTA TATGGGGCAC CCCCGCCCCT   601 TGTAAACTTC CCTGACCCTG ACATGACNNN ANTTACTAAC AGCCCCTCTC TCCAAGCTCA   661 CTTNNNNGCT CTCTACTTAG TCCANCNCGA AGTCTGGAGA NNNNNGGCGG CAGCCTACCA   721 AGAACANNNN NNCCGACCGG NGGNNNCTCA CCCTTANCGA GTCGGCNANN NNNNGTGGGT   781 CCGCCGANAC CANANNNNNA ANCNNNAACC TCGCTGGAAA GGANCTTACN NNNTCCTGCT   841 GANCACCCNN NNNNNCCCTC NAANNANANN GNNTCNCANC TNGNNNNCNN NNCNNCCCAC 901 NTNAANGNTG NCNNNCNNNG NGGNNNNNNN NNN //
Primer: IRES-F (Pr0036) Region: IRES,puroR Sequence file: RDB19829_B2Lgg.seq
>D08693C3_B2Lg_3_IRES-F_A01_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNT NNGTNNNNNT NNNNNNNNNN TCTAGGCCCC CCGAACCACG    61 GGGANGTGGT TTTCCTTTGA AAAACACGAT GATAATATGG CCACAACCAT GGTTACCGAG   121 TACAAGCCCA CGGTGCGCCT CGCCACCCGC GACGACGTCC CCAGGGCCGT ACGCACCCTC   181 GCCGCCGCGT TCGCCGACTA CCCCGCCACG CGCCACACCG TCGATCCGGA CCGCCACATC   241 GAGCGGGTCA CCGAGCTGCA AGAACTCTTC CTCACGCGCG TCGGGCTCGA CATCGGCAAG   301 GTGTGGGTCG CGGACGACGG CGCCGCGGTG GCGGTCTGGA CCACGCCGGA GAGCGTCGAA   361 GCGGGGGCGG TGTTCGCCGA GATCGGCCCG CGCATGGCCG AGTTGAGCGG TTCCCGGCTG   421 GCCGCGCAGC AACAGATGGA AGGCCTCCTG GCGCCGCACC GGCCCAAGGA GCCCGCGTGG   481 TTCCTGGCCA CCGTCGGCGT CTCGCCCGAC CACCAGGGCA AGGGTCTGGG CAGCGCCGTC   541 GTGCTCCCCG GAGTGGAGGC GGCCGAGCGC GCCGGGGTGC CCGCCTTCCT GGAGACCTCC   601 GCGCCCCGCA ACCTCCCCTT CTACGAGCGG CTCGGCTTCA CCGTCACCGC CGACGTCGAG   661 GTGCCCGAAG GACCGCGCAC CTGGTGCATG ACCCGCAAGC CCGGTGCCTG AGTCGACGAT   721 AAAATAAAAG ANTTTATTTA GTCTCCNGAA AAAGGGGGGA ATGNAAAGAC CCCACCTGTA   781 GGTTTGGCAA GCTAGCTNAA NTAACGCCAT TTTGCAAGGC ATGGAAAAAT ACATNACTGA   841 NAATANAGAA NTTCANATCN NGGTCAGGAA NNNATNGNAC AGCTGAATNT GGGCCNNACA   901 GGATATCTGT GGTAAGCAGT TNCTGNCCCG GCNNNNGNCC AANAACNGAT GGAACAGCTG   961 AATATGGNNN NNANNNGANN TCNGTGGTAN NCAGTTNCTN NCCCNGNTNN NGNCCNANNN  1021 NNNATNGNNN NNNNNANGNN GNCNGNNNNN NNNNANTTTN NNNNNNNAAN NANNNNANNG 1081 TTNNNNNNGG GNNNNNCCCN NNGNNNNNNN ANNNNNCCNN NNNNNNNN //
Primer: PuroR_F (Pr0554) Region: 3' LTR Sequence file: RDB19829_B2Lgh.seq
>D08693C3_B2Lg_3_PuroR_F_C01_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNGNNC GCGCACCTGG TGCNTGACCC    61 GCAAGCCCGG TGCCTGNNTC NANGATAAAA TAAAAGATTT TATTTAGTCT CCAGAAAAAG   121 GGGGGAATGA AAGACCCCAC CTGTAGGTTT GGCAAGCTAG CTTAAGTAAC GCCATTTTGC   181 AAGGCATGGA AAAATACATA ACTGAGAATA GAGAAGTTCA GATCAAGGTC AGGAACAGAT   241 GGAACAGCTG AATATGGGCC AAACAGGATA TCTGTGGTAA GCAGTTCCTG CCCCGGCTCA   301 GGGCCAAGAA CAGATGGAAC AGCTGAATAT GGGCCAAACA GGATATCTGT GGTAAGCAGT   361 TCCTGCCCCG GCTCAGGGCC AAGAACAGAT GGTCCCCAGA TGCGGTCCAN CCCTCAGCAG   421 TTTCTAGAGA ACCATCAGAT GTTTCCAGGG TGCCCCAAGG ACCTGAAATG ACCCTGTGCC   481 TTATTTGAAC TAACCAATCA GTTCGCTTCT CGCTTCTGTT CGCGCGCTTC TGCTCCCCGA   541 GCTCAATAAA AGAGCCCACA ACCCCTCACT CGGGGCGCCA GTCCTCCGAT TGACTGAGTC   601 GCCCGGGTAC CCGTGTATCC AATAAACCCT CTTGCAGTTG CATCCGACTT GTGGTCTCGC   661 TGTTCCTTGG GAGGGTCTCC TCTGAGTGAT TGACTACCCG TCNNNGGGGG TCTTTCACAT   721 GCANNNNGNA TCNAAATTAA TTTGGTTTTT TTTCTTAAGT ATTTACNTTA AATGGCCATA   781 GTTGCNTTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGGCGCTCTT   841 CCGCTTCCTC GCTCNCTGAC TCGCTGCNCT CGGTCGTTCN GGCTGCNGNN ANNNNNNNNC   901 NGCTNNCTCN AANGCGGGTA ATNNNGGTNA TCNNCANNAT CNGGGGATAN NNNANGAAAA   961 GAACATGTGA NCNAANNGNC NNNNNAANGN NNNNNANCNN TAAAANNGNC NNNNNTNNCT 1021 NGGCNNTTTT NNNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

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