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pME18s-HA-hFIP200 (#RDB19769)

Expression vector of human FIP200 with N-terminal HA.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Hosokawa, N., Mol. Biol. Cell 20 (7): 1981-1991 (2009). PMID 19211835. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Expression vector of human FIP200 with N-terminal HA.
Vector backbone pME18s (plasmid)
Size of vector backbone 3.4 kb
Selectable markers Ampicillin
Growth conditions LB+Amp, 37oC
Gene/insert name human RB1CC1 cDNA; synthetic HA-tag.
Depositor|Developer Mizushima, Noboru |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Hosokawa, N. et al. Mol. Biol. Cell. 20(7): 1981-1991, 2009)
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Hosokawa, N. et al. Mol. Biol. Cell. 20(7): 1981-1991, 2009)。

Catalog # Resource name Shipping form Fee
RDB19769 pME18s-HA-hFIP200 DNA solution

How to cite this biological resource

Materials & Methods section:

The pME18s-HA-hFIP200 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19769).

Reference section:

Hosokawa, N., Hara, T., Kaizuka, T., Kishi, C., Takamura, A., Miura, Y., Iemura, S., Natsume, T., Takehana, K., Yamada, N., Guan, J.L., Oshiro, N., Mizushima, N., Nutrient-dependent mTORC1 association with the ULK1-Atg13-FIP200 complex required for autophagy. Mol. Biol. Cell 20 (7): 1981-1991 (2009). PMID 19211835. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Hosokawa, N., Nutrient-dependent mTORC1 association with the ULK1-Atg13-FIP200 complex required for autophagy. Mol. Biol. Cell 20 (7): 1981-1991 (2009). PMID 19211835. [PubMed] [Article] [RRC of NBRP]