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p3xFLAG-CMV10-hUVRAG

Transient expression vector of human UVRAG with N-terminal 3×FLAG.

Catalog number RDB19776
Resource name p3xFLAG-CMV10-hUVRAG
Clone info. Transient expression vector of human UVRAG with N-terminal 3×FLAG.
Vector backbone p3xFLAG-CMV-10 (plasmid)
Size of vector backbone 6.3 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Gene/insert name human UVRAG cDNA; synthetic 3×FLAG-tag.
Depositor|Developer Mizushima, Noboru |
 
Sequence (full) RDB19776hts01.seqcheck assembled from experimentally sequenced data
Test sheet RDB19776_B2Jlp1-1.pdf 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Itakura, E. et al. Mol. Biol. Cell. 19(12): 5360-5372, 2008)
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提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Itakura, E. et al. Mol. Biol. Cell. 19(12): 5360-5372, 2008)。
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19776 p3xFLAG-CMV10-hUVRAG DNA solution

How to cite this biological resource

Materials & Methods section:

The p3xFLAG-CMV10-hUVRAG was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19776).

Reference section:

Itakura, E., Kishi, C., Inoue, K., Mizushima, N., Beclin 1 forms two distinct phosphatidylinositol 3-kinase complexes with mammalian Atg14 and UVRAG. Mol. Biol. Cell 19 (12): 5360-5372 (2008). PMID 18843052. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Itakura, E., Beclin 1 forms two distinct phosphatidylinositol 3-kinase complexes with mammalian Atg14 and UVRAG. Mol. Biol. Cell 19 (12): 5360-5372 (2008). PMID 18843052. [link to RRC of NBRP]