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pMRXIP-GFP-Stx17TM (#RDB19793)

Retroviral vector for stable expression of human Stx17TM with N-terminal GFP.


Drawn by SnapGene® software
Sequence (full) RDB19793hts01.seq
Publication Itakura, E., Cell 151 (6): 1256-1269 (2012). [link to RRC of NBRP]
Test sheet RDB19793_B2Jlp1-1.pdf 
Assembled from experimentally sequenced data.
 
Clone info. Retroviral vector for stable expression of human Stx17TM with N-terminal GFP. See Table S1 of Itakura, E. et al., Cell, 151 (6): 1256-1269, 2012.
Vector backbone pMRXIP GFP (plasmid)
Selectable markers Ampicillin (E. coli), Puromycin (mammalian cell)
Gene/insert name human STX17 cDNA; Aequorea victoria GFP (EGFP).
Depositor|Developer Mizushima, Noboru |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Itakura, E. et al. Cell 151(6): 1256-1269, 2012)
Additional terms and conditions:
Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Itakura, E. et al. Cell 151(6): 1256-1269, 2012)。
MTAに書く付加的使用条件：
pMRXベクターについて：利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19793 pMRXIP-GFP-Stx17TM DNA solution

How to cite this biological resource

Materials & Methods section:

The pMRXIP-GFP-Stx17TM was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19793).

Reference section:

Itakura, E., Kishi-Itakura, C., Mizushima, N., The hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes. Cell 151 (6): 1256-1269 (2012). PMID 23217709. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Itakura, E., The hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes. Cell 151 (6): 1256-1269 (2012). PMID 23217709. [link to RRC of NBRP]