Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMRX-IP-GFP-LC3-RFP-LC3 delta G

Fluorescent probe to evaluate autophagic flux by the rat LC3 fused with GFP and RFP.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	(1) BIOLOGICAL RESOURCE is limited to for academic research in non-profit organization. (2) The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. (3) Any use of the BIOLOGICAL RESOURCE for profit purposes by a non-profit organization or any use of the BIOLOGICAL RESOURCE by a profit organization requires a separate license from the DEPOSITOR prior to distribution. (4) In publishing research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the original publication designated by the DEPOSITOR (Kaizuka, T. et al. Mol. Cell, 64 (4): 835-849, 2016) and an acknowledgment to the DEPOSITOR are requested. (5) The RECIPIENT must contact the DEPOSITOR in case of application for any patents or commercial use with the results from the use of BIOLOGICAL RESOURCE.
Additional terms and conditions for distribution	Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
Ordering	Please visit Information of Request for Distribution.[link] UCSD's MTA is also required. Please visit Additional Forms section. Order form [Credit Card Payment]  [Bank Transfer or Check Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Schedule A (GFP-A) [Link] Approval form by depositor [Word]
Remarks	((Additional form)) Approval Form(FormD) Remember that you will be working with samples containing infectious virus. UCSD's MTA is also required. Please visit Additional Forms section. Please use Schedule A (GFP-A) additionally. Please obtain written approval of the depositor.

Distribution information (domestic users) [open/close]

提供条件	1. 非営利団体による学術研究に限る。2. 利用者は事前に寄託者の提供承諾書を得る。3. 当該リソースの非営利団体の営利目的での利用および営利団体の利用については、事前に寄託者との間で、別途使用許諾に係る契約の締結が必要である。4. 研究成果の公表にあたって寄託者の指定する文献(Kaizuka, T. et al. Mol. Cell, 64 (4): 835-849, 2016)を引用し、謝辞の表明を必要とする。5. 利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
付加的提供条件	pMRXベクターについて：利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。別途、UCSDとのMTAも必要です。「申込みに必要な書式」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] Schedule A（GFP-A, 英語版のみ） [Link] 寄託者による提供承諾書 [Word]
備考	【追加提供依頼書】承諾書(FormD) このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 別途、UCSDとのMTAも必要です。「申込みに必要な書式」をご覧ください。 Schedule A（GFP-A, 英語版のみ）が追加で必要です。 寄託者の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14600_A7Bap1-4.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C Region: insert mid (ratLC3, mRFP1 5) Sequence file: RDB14600_A7Baa.seq
>D04280A1_A7Ba_1_EGFP-C_A04_01_ABI24.ab1     1 ACGGGGGCAC ATCTCGGCAT GGACGAGCTG TACAGGGTGG CGGTGGCTCG GGCGGTGGTG    61 GGTCGGGTGG CGGCGGATCT ATCGATGAAT TGAATTCCAT GCCGTCCGAG AAGACCTTCA   121 AACAGCGCCG GAGCTTCGAA CAAAGAGTGG AAGATGTCCG GCTCATCCGG GAGCAGCACC   181 CCACCAAGAT CCCAGTGATT ATAGAGCGAT ACAAGGGTGA GAAGCAGCTG CCCGTCCTGG   241 ACAAGACCAA GTTCCTTGTA CCTGATCACG TGAATATGAG CGAACTCATC AAGATAATTA   301 GAAGGCGCCT GCAGCTCAAT GCTAACCAAG CCTTCTTCCT CCTGGTGAAT GGGCACAGCA   361 TGGTGAGTGT GTCCACACCC ATCTCTGAAG TGTACGAGAG CGAGAGAGAT GAAGACGGCT   421 TCCTGTACAT GGTCTATGCC TCCCAGGAGA CGTTCGGGAT GGCCTCCTCC GAGGACGTCA   481 TCAAGGAGTT CATGCGCTTC AAGGTGCGCA TGGAGGGCTC CGTGAACGGC CACGAGTTCG   541 AGATCGAGGG CGAGGGCGAG GGCCGCCCCT ACGAGGGCAC CCAGACCGCC AAGCTGAAGG   601 TGACCAAGGG CGGCCCCCTG CCCTTCGCCT GGGACATCCT GTCCCCTCAG TTCCAGTACG   661 GCTCCAAGGC CTACGTGAAG CACCCCGCCG ACATCCCCGA CTACTTGAAG CTGTCCTTCC   721 CCGAGGGCTT CAAGTGGGAG CGCGTGATGA ACTTCGAGGA CGGCGGCGTG GTGACCGTGA   781 CCCAGGACTC CTCCCTGCAG GACGGCGAGT TCATCTACAA GTGAAGCTGC GCGGCACCAA   841 CTTCCCCTCC GACGGCCCCG TAATGCAGAA GAGACCATGG GCTGGGAGGC CTCCACGAGC   901 CGGATGTTAC CCCGAGGACG GCGCCCTGAG GCGAGATCAG ATGAGCTGAA GCTGAAGGAC   961 GGCGGCACTA CGACGCGAGG TCAGACCACT ACATGGCCAG AGCCGGTGCA GCTGCCCGGC  1021 GCTACAAGAA CGGACTCAAG CTGGAACATC ACCTCCCACA ACGAAGACTA CACCAATCGT  1081 GAACAGTACG AGGCGGCGAG GGCGCCACTC ACGGCATCAT GAATTGAATC ATGGCTCGGA 1141 AGACTCAACG GCCGACTGAA CAAGTGATAA GTTCCGTCAT CTGGCAC //
Primer: mRFP1-inner1 Region: insert mid (mRFP1 3', ratLC3deltaG, IRES) Sequence file: RDB14600_A7Bab.seq
>D04280A1_A7Ba_1_mRFP1-inner1_E10_13_ABI24.ab1     1 GCGCGAGGGG GGGGCTGACG TGACCCAGGA CTCCTCCCTG CAGGACGGCG AGTTCATCTA    61 CAAGGTGAAG CTGCGCGGCA CCAACTTCCC CTCCGACGGC CCCGTAATGC AGAAGAAGAC   121 CATGGGCTGG GAGGCCTCCA CCGAGCGGAT GTACCCCGAG GACGGCGCCC TGAAGGGCGA   181 GATCAAGATG AGGCTGAAGC TGAAGGACGG CGGCCACTAC GACGCCGAGG TCAAGACCAC   241 CTACATGGCC AAGAAGCCCG TGCAGCTGCC CGGCGCCTAC AAGACCGACA TCAAGCTGGA   301 CATCACCTCC CACAACGAGG ACTACACCAT CGTGGAACAG TACGAGCGCG CCGAGGGCCG   361 CCACTCCACC GGCGCCATCG ATGAATTGAA TTCCATGCCG TCCGAGAAGA CCTTCAAACA   421 GCGCCGGAGC TTCGAACAAA GAGTGGAAGA TGTCCGGCTC ATCCGGGAGC AGCACCCCAC   481 CAAGATCCCA GTGATTATAG AGCGATACAA GGGTGAGAAG CAGCTGCCCG TCCTGGACAA   541 GACCAAGTTC CTTGTACCTG ATCACGTGAA TATGAGCGAA CTCATCAAGA TAATTAGAAG   601 GCGCCTGCAG CTCAATGCTA ACCAAGCCTT CTTCCTCCTG GTGAATGGGC ACAGCATGGT   661 GAGTGTGTCC ACACCCATCT CTGAAGTGTA CGAGAGCGAG AGAGATGAAG ACGGCTTCCT   721 GTACATGGTC TATGCCTCCC AGGAGACGTT CTAAGGATCC CAGTGTGGTG GTACGGGAAT   781 TCCTGCAGGC CTCGAGGGCC GGCGCGCCGC GGCCGCTACG TAAATTCCGC CCCCCCCCCC 841 CCTCTCCCTC //
Primer: MMLV-pol_f2 Region: pol region, insert 5' (EGFP, ratLC3 5') Sequence file: RDB14600_A7Bac.seq
>D04280A1_A7Ba_1_MMLV-pol_F2_C04_07_ABI24.ab1     1 TACGGGGGGG GTGGACATCC TCTAGACTGC CGGATCTCCC GCCGCCACCA TGGTGAGCAA    61 GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA   121 CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC   181 CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC   241 CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC AGCACGACTT   301 CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA   361 CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT   421 CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA   481 CAACTACAAC AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG GCATCAAGGT   541 GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG ACCACTACCA   601 GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCAC   661 CCAGTCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT   721 CGTGACCGCC GCCGGGATCA CTCTCGGCAT GGACGAGCTG TACAAGGGTG GCGGTGGCTC   781 GGGCGGTGGT GGGTCGGGTG GCGGCGGATC TATCGATGAA TTGAATTCCA TGCCGTCCGA   841 GAAGACCTTC AAACAGCGCC GGAGCTTCGA ACAAAGAGTG GAAGATGTCC GGCTCATCCG   901 GGAGCAGCAC CCCACCAAGA TCCCAGTGAT TATAGAGCGA TACAAGGGTG AGAAGCAGCT   961 GCCCGTCCTG ACAGACCAAG TTCCTTGTAC CTGATCACGT GAATATGAGC GAACTCATCA  1021 AGATAATTTA GAAGCGCTGC AGCTCAATGC TAACCAAGCC TTCTTCTCTG CTGATGGGCA  1081 CAGCATGTGA GTGTGTCCAC ACCCATCTCT GAGTGTACGA AGCGAGAGAT GAGACGCTCC 1141 TGTACATGTC TATGCTCCAG GAGAAACGTT CCGG //
Primer: EGFP-N Region: insert 5' (EGFP 5', pol region, gag, MMLV psi) Sequence file: RDB14600_A7Bad.seq
>D04280A1_A7Ba_1_EGFP-N_E04_13_ABI24.ab1     1 GAATCAACCC GGGTTTAAAG CTCCTCGCCC TTGCTCACCA TGGTGGCGGC GGGAGATCCG    61 GCAGTCTAGA GGATGGTCCA CCCCCGGGGT CGGCAGCCTT CACGTGGGCG GCGTGTATCC   121 AAGCTGCGAT GCCGTCTACT TTGAGGGCGG TGGGGGTGGT CAGCAGGACT GTGTAAGGTC   181 CTTTCCAGCG AGGTTCTAGG TTCTTAGTCT GGTGTCGGCG GACCCACACT GTGTCGCCGA   241 CTCGGTAAGG GTGAGGTACC ACCGGTCGGT CCAGTTGTTC TTGGTAGGCT GCCGCCAGAG   301 GTCTCCAGAC TTCGTGCTGG ACTAAGTAGA GAGCCTGTAA GTGAGCTTGG AGAGAGGGGC   361 TGTTAGTAAC TCTTGTCATG TCAGGGTCAG GGAAGTTTAC AAGGGGCGGG GGTGCCCCAT   421 ATAAGATCTC ATATGGCCAT ATGGGGGCGC CTAGAGAAGG AGTGAGGGCT GGATAAAGGG   481 AGGATCGAGG CGGGGTCGAA CGAGGAGGTT CAAGGGGGAG AGACGGGGCG GATGGAGGAA   541 GAGGAGGCGG AGGCTTAGGG TGTACAAAGG GCTTGACCCA GGGAGGGGGG TCAAAAGCCA   601 AGGCTTCCCA GGTCACGATG TAGGGGACCT GGTCTGGGTG TCCATGCGGG CCAGGTGAAA   661 AGACCTTGAT CTTAACCTGG GTGATGAGGT CTCGGTTAAA GGTGCCGTCT CGCGGCCATC   721 CGACGTTAAA GGTTGGCCAT TCTGCAGAGC AGAAGGTAAC CCAACGTCTC TTCTTGACAT   781 CTACCGACTG GTTGTGAGCG ATCCGCTCGA CATCTTTCCA GTGACCTAAG GTCAAACTTA   841 AGGGAGTGGT AACAGTCTGG CCCGGGCCCA TATTTTCAGA CAAATACAGA AACACAGTCA   901 GACAGAGACA ACACAGAACG ATGCTGCAGC AGACAAGACG CGCGGCGCGG CTTCGGTCCC   961 AAACCGAAAG CAAAAAATTC AGACGGAGGC GGGAACTGTT TTAAGTTCTC GTCTCCTACC  1021 AGGAACCAAC AATTATCCCT CCTCTAAGGG GGGGTGCACC CAAAGAAAGT CCAAAACCGA  1081 TTCGGGAATT TTTGGAACCT CAAGTCGGGG CCACAAAAAC GGCCCTCGAA GTCCCTGGGA  1141 ACGTCTCAGA TGCCGCGGGG TTTCGAACTC GTTCAGGTTC CCACCAACCG GGGGTCGCGC 1201 CCCCGGATAT //
Primer: pMXs-3LTRup_R2 Region: PuriR, IRES Sequence file: RDB14600_A7Bae.seq
>D04280A1_A7Ba_1_pMXs-3LTRup_R2_G04_19_ABI24.ab1     1 GGCATATAAA ATCTTTTATT TTATCGTCGA GCCATGGGGT CGTGCGCTCC TTTCGGTCGG    61 GCGCTGCGGG TCGTGGGGCG GGCGTCAGGC ACCGGGCTTG CGGGTCATGC ACCAGGTGCG   121 CGGTCCTTCG GGCACCTCGA CGTCGGCGGT GACGGTGAAG CCGAGCCGCT CGTAGAAGGG   181 GAGGTTGCGG GGCGCGGAGG TCTCCAGGAA GGCGGGCACC CCGGCGCGCT CGGCCGCCTC   241 CACTCCGGGG AGCACGACGG CGCTGCCCAG ACCCTTGCCC TGGTGGTCGG GCGAGACGCC   301 GACGGTGGCC AGGAACCACG CGGGCTCCTT GGGCCGGTGC GGCGCCAGGA GGCCTTCCAT   361 CTGTTGCTGC GCGGCCAGCC GGGAACCGCT CAACTCGGCC ATGCGCGGGC CGATCTCGGC   421 GAACACCGCC CCCGCTTCGA CGCTCTCCGG CGTGGTCCAG ACCGCCACCG CGGCGCCGTC   481 GTCCGCGACC CACACCTTGC CGATGTCGAG CCCGACGCGC GTGAGGAAGA GTTCTTGCAG   541 CTCGGTGACC CGCTCGATGT GGCGGTCCGG GTCGACGGTG TGGCGCGTGG CGGGGTAGTC   601 GGCGAACGCG GCGGCGAGGG TGCGTACGGC CCGGGGGACG TCGTCGCGGG TGGCGAGGCG   661 CACCGTGGGC TTGTACTCGG TCATGGTTGT GGCCATATTA TCATCGTGTT TTTCAAAGGA   721 AAACCACGTC CCCGTGGTTC GGGGGGCCTA GACGTTTTTT TAACCTCGAC TAAACACATG   781 TAAAGCATGT GCACCGAGGC CCCAGATCAG ATCCCATACA ATGGGGTACC TTCTGGGCAT   841 CCTTCAGCCC CTTGTTGAAT ACGCTTGAGG AGAGCCATTT GACTCTTTCC ACAACTATCC   901 AACTCACAAC GTGGCACTGG GGTTGTGCCG CCTTTGCAGG TGTATCTTAT ACACGTGGCT   961 TTTGGGCCGC AGAGGCACCT GTCGCCAGGG TGGGGGGTTC CGCTGCCTGC AAAGGGTCGC  1021 TACAGACGTT GTTTGTCTTC AGAGCTTCCA GAGGAACTGC TTCTTCACGA CATTCAACAG  1081 ACCTTGCATT CCTTGCGAGA GGGAAGACCC CTAGGATGGC TCGTCAGAGA CCGTCAGTAT 1141 TCGGCCTCAC ATTGCCTAAA GAAACGGCCA AATATG //
Primer: p15Aori_F Region: Ori, LTR Sequence file: RDB14600_A7Baf.seq
>D04280A1_A7Ba_1_p15Aori_F_F04_16_ABI24.ab1     1 GCAGGGACGT GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC    61 AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT   121 GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT   181 CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA   241 ATACGCAAAC CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAACTA TGGCCATTTA   301 ATGTAAATAC TTAAGAAAAA AAACCAAATT AATTTTGATA CATGCTGCAT GTGAAAGACC   361 CCCGCTGACG GGTAGTCAAT CACTCAGAGG AGACCCTCCC AAGGAACAGC GAGACCACAA   421 GTCGGATGCA ACTGCAAGAG GGTTTATTGG ATACACGGGT ACCCGGGCGA CTCAGTCAAT   481 CGGAGGACTG GCGCCCCGAG TGAGGGGTTG TGGGCTCTTT TATTGAGCTC GGGGAGCAGA   541 AGCGCGCGAA CAGAAGCGAG AAGCGAACTG ATTGGTTAGT TCAAATAAGG CACAGGGTCA   601 TTTCAGGTCC TTGGGGCACC CTGGAAACAT CTGATGGTTC TCTAGAAACT GCTGAGGGCT   661 GGACCGCATC TGGGGACCAT CTGTTCTTGG CCCTGAGCCG GGGCAGGAAC TGCTTACCAC   721 AGATATCCTG TTTGGCCCAT ATTCAGCTGT TCCATCTGTT CTTGGCCCTG AGCCGGGGCA   781 GGAACTGCTT ACCACAGATA TCCTGTTTGG CCCATATTCA GCTGTTCCAT CTGTTCCTGA   841 CCTTGATCTG AACTTCTCTA TTCTCAGTTA TGTATTTTTC CATGCCTTGC AAAATGGCGT   901 TACTTAAGCT AGCTTGCCAA ACCTACAGGT GGGGTCTTTC ATTCCCCCCT TTTTCTGGAG   961 ACTAAATAAA ATCTTTTATT TTATCGTCGA GCCATGGGGT CGTGCGCTCC TTTCGGTCGG  1021 GCGCTGCGGG TCGTGGGGCG GGGCGTCAGG CACCGGGCTT GCGGGTCATG CACCAGTGCG  1081 CGTCCTCCGG CACCTCGACG TCGCGTGACG TGAAGCCGAG TCGCTCGTTA GAGGAGTTGC 1141 GGACGCGGAG TCTCTCCAGG AATGC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2020.11.13

GNP_filter3_RDBDEP_html_201104.pl