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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMRX-IP-GFP-LC3-RFP-LC3 delta G

Fluorescent probe to evaluate autophagic flux by the rat LC3 fused with GFP and RFP.

Catalog number RDB14600
Resource name pMRX-IP-GFP-LC3-RFP-LC3 delta G
Clone info. Fluorescent probe to evaluate autophagic flux. Expression vector of rat microtubule-associated protein 1 light chain 3 beta (LC3) fused with GFP and RFP. This probe is cleaved by endogenous ATG4 proteases into equimolar amounts of GFP-LC3 and RFP-LC3deltaG. The GFP-LC3 is degraded by autophagy, while RFP-LC3deltaG remains in the cytosol as an internal control. Thus, autophagic flux can be estimated by calculating the GFP/RFP signal ratio. Please refer DISCUSSION section of Kaizuka, T., Mol. Cell 64, 835-849, 2016 for the difference between pMRX-IP-GFP-LC3-RFP and this clone.
Comment Expression was confirmed by the depositor with immunoblotting and fluorescent microscopy. Please refer Saitoh, T. et al., FEBS Lett., 532 (1-2): 45-51, (2002) for the construction of pMRX-IP vector.
Vector backbone pMRX-IP (Plasmid)
Selectable markers Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name Rat LC3 cDNA Aequorea victoria GFP cDNA Discosoma sp. RFP cDNA
Depositor|Developer Mizushima, Noboru |
Other clones in our bank

External Database
Rat LC3 Aequorea victoria GFP
    Discosoma sp. RFP

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution (1) BIOLOGICAL RESOURCE is limited to for academic research in non-profit organization. (2) The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. (3) Any use of the BIOLOGICAL RESOURCE for profit purposes by a non-profit organization or any use of the BIOLOGICAL RESOURCE by a profit organization requires a separate license from the DEPOSITOR prior to distribution. (4) In publishing research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the original publication designated by the DEPOSITOR (Kaizuka, T. et al. Mol. Cell, 64 (4): 835-849, 2016) and an acknowledgment to the DEPOSITOR are requested. (5) The RECIPIENT must contact the DEPOSITOR in case of application for any patents or commercial use with the results from the use of BIOLOGICAL RESOURCE.
          Additional terms and conditions:
          Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.

          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Approval form by depositor [Word]
          Remarks ((Additional form)) Approval Form(FormD)
          Remember that you will be working with samples containing infectious virus.
          This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
          Please obtain written approval of the depositor.
          提供案内 (日本国内) [open/close]

          提供条件 1. 非営利団体による学術研究に限る。2. 利用者は事前に寄託者の提供承諾書を得る。3. 当該リソースの非営利団体の営利目的での利用および営利団体の利用については、事前に寄託者との間で、別途使用許諾に係る契約の締結が必要である。4. 研究成果の公表にあたって寄託者の指定する文献(Kaizuka, T. et al. Mol. Cell, 64 (4): 835-849, 2016)を引用し、謝辞の表明を必要とする。5. 利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
          付加的提供条件:
          pMRXベクターについて:利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          寄託者による提供承諾書 [Word]
          備考 【追加提供依頼書】承諾書(FormD)
          このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
          Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。
          寄託者の書面による提供承諾が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB14600 pMRX-IP-GFP-LC3-RFP-LC3 delta G DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pMRX-IP-GFP-LC3-RFP-LC3 delta G was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14600).

          Reference section:

          Morishita, H., Kaizuka, T., Hama, Y., Mizushima, N., A new probe to measure autophagic flux in vitro and in vivo. Autophagy 13 (4): 757-758 (2017). PMID 28121224.
          Kaizuka, T., Morishita, H., Hama, Y., Tsukamoto, S., Matsui, T., Toyota, Y., Kodama, A., Ishihara, T., Mizushima, T., Mizushima, N., An Autophagic Flux Probe that Releases an Internal Control. Mol Cell. 2016 Nov 17;64(4):835-849 (2016). PMID 27818143.

          Further references such as user reports and related articles (go to bottom)


          References and tips

          Featured content

          Featured content Dnaconda's recommendation EXR0088e (English text)
          Featured content Dnaconda's recommendation EXR0088j (Japanese text)
          Featured content Autophagy (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14600_A7Bap1-5.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: MMLV-pol_F2 (Pr0364)
          Region: insert 5'(EGFP 5')
          Sequence file: RDB14600_A7Bag.seq check
          >D04280A1_A7Ba_1_MMLV-pol_F2_C04_07_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNCC NCNNNNNTGC CGGNNCTCCC GCCGCCNCCA TGGTGAGCAA
             61 GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA
            121 CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC
            181 CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC
            241 CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC AGCACGACTT
            301 CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA
            361 CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT
            421 CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA
            481 CAACTACAAC AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG GCATCAAGGT
            541 GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG ACCACTACCA
            601 GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCAC
            661 CCAGTCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT
            721 CGTGACCGCC GCCGGGATCA CTCTCGGCAT GGACGANCTG TACAAGGNTG GCGGTGGCTC
            781 GNGCGGTGGT GNGTCGGGTG GCGGCGGNTC TATCGATGAA TTGAATTCCN TGCCGTCNGA
            841 NAAGANCTTC NAACAGCGCC NGANCTNCNA ACAAAGANTG GAANANGNCC GGNTNNTNCN
            901 GGNNCANCAC CCCNCCAANA TCCNNGNGNN TATANANCGA TACNNGGNNG ANAANCANCT
            961 GCCNNNCNNN NNNNANNNNN TTNNNNGNNN NNGNNNNCGT NNNNNTGANC NNNNTCNTCN
          1021 NNANNANTNN NNNNNNNN
          //
          Primer: mRFP1-C_F (Pr0502)
          Region: insert 3'(rat LC3 deltaG),IRES
          Sequence file: RDB14600_A7Bah.seq check
          >14600_14600_B2FhF_1_mRFP1-C_F_B01_02_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NCTGGNNNTC NCCTCCCNCA ACGAGGACTA CACCATCGTG
             61 GAACAGTACG AGCNCNCCNA GGGCCGCCAC TCCACCGGCG CCATCGATGA ATTGAATTCC
          121 ATGCCGTCCG AGNNNNNNNN NN
          //
          Primer: mVenus-M_F (Pr0574)
          Region: insert mid(EGFP 3',rat LC3,mRFP1 5')
          Sequence file: RDB14600_A7Bai.seq check
          >14600_14600_B2FhF_mVenus-M_F_B06_06_ABI24.ab1
              1 NNNNNNNNNN NNNNNGGCAT CNNGGTGNAC TTNNAGANCC GCCNCAACAT CGAGGACGGC
             61 AGCGTGCAGC TCGCCGACCA CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG
            121 CTGCCCGACA ACCACTACCT GAGCACCCAG TCCGCCCTGA GCAAAGACCC CAACGAGAAG
            181 CGCGATCACA TGGTCCTGCT GGAGTTCGTG ACCGCCGCCG GGATCACTCT CGGCATGGAC
            241 GAGCTGTACA AGGGTGGCGG TGGCTCGGGC GGTGGTGGGT CGGGTGGCGG CGGATCTATC
            301 GATGAATTGA ATTCCATGCC GTCCGAGAAG ACCTTCAAAC AGCGCCGGAG CTTCGAACAA
            361 AGAGTGGAAG ATGTCCGGCT CATCCGGGAG CAGCACCCCA CCAAGATCCC AGTGATTATA
            421 GAGCGATACA AGGGTGAGAA GCAGCTGCCC GTCCTGGACA AGACCAAGTT CCTTGTACCT
            481 GATCACGTGA ATATGAGCGA ACTCATCAAG ATAATTAGAA GGCGCCTGCA GCTCAATGCT
            541 AACCAAGCCT TCTTCCTCCT GGTGAATGGG CACAGCATGG TGAGTGTGTC CACACCCATC
            601 TCTGAAGTGT ACGAGAGCGA GAGAGATGAA GACGGCTTCC TGTACATGGT CTATGCCTCC
            661 CAGGAGACGT TCGGGATGGC CTCCTCCGAG GANGTCATCA AGGAGTTCAT GCGCTTCAAG
            721 GTGCGCATGG ANGGCTCCGT GAACGGNCAC GAGTTCGAGA TCGANGGNGA NGGCGANGGN
            781 CGCCCCTACG AGGGCACCCA GACCGCCNAG CTGAANGTGA NCAANGGCGG NCCCCTGCCC
            841 TTCNCCNGGG ACATCCNGNC CCCTCNGTTC CAGTACNGNT CCNAGGNCTA CGTGAAGCAN
            901 CCCGCCNANN TCCCNNANNA CTTGAAGCTG TCCTTCCCCG ANGGCTTCNA GNNNNNNCNC
            961 GNGNTGAACT TCGANGANNN NNNCNNNNNG ANNNNNNNCC NNNNTNNTNC CNGNNNNNNN
          1021 NNN
          //
          Primer: mRFP1-inner1 (Pr0443)
          Region: insert mid(mRFP1 3',rat LC3 deltaG 5')
          Sequence file: RDB14600_A7Baj.seq check
          >D04280A1_A7Ba_1_mRFP1-inner1_E10_13_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNCNNNNN NTCCTCCCTG NNGGNNGGCG AGTTCATCTA
             61 CAAGGTGAAG CTGCGCGGCA CCAACTTCCC CTCCGACGGC CCCGTAATGC AGAAGAAGAC
            121 CATGGGCTGG GAGGCCTCCA CCGAGCGGAT GTACCCCGAG GACGGCGCCC TGAAGGGCGA
            181 GATCAAGATG AGGCTGAAGC TGAAGGACGG CGGCCACTAC GACGCCGAGG TCAAGACCAC
            241 CTACATGGCC AAGAAGCCCG TGCAGCTGCC CGGCGCCTAC AAGACCGACA TCAAGCTGGA
            301 CATCACCTCC CACAACGAGG ACTACACCAT CGTGGAACAG TACGAGCGCG CCGAGGGCCG
            361 CCACTCCACC GGCGCCATCG ATGAATTGAA TTCCATGCCG TCCGAGAAGA CCTTCAAACA
            421 GCGCCGGAGC TTCGAACAAA GAGTGGAAGA TGTCCGGCTC ATCCGGGAGC AGCACCCCAC
            481 CAAGATCCCA GTGATTATAG AGCGATACAA GGGTGAGAAG CAGCTGCCCG TCCTGGACAA
            541 GACCAAGTTC CTTGTACCTG ATCACGTGAA TATGAGCGAA CTCATCAAGA TAATTAGAAG
            601 GCGCCTGCAG CTCAATGCTA ACCAAGCCTT CTTCCTCCTG GTGAATGGGC ACAGCATGGT
            661 GAGTGTGTCC ACACCCATCT CTGAAGTGTA CGAGAGCGAG AGAGATGAAG ACGGCTTCCT
            721 GTACATGGTC TATGCCTCCC AGGANACGTT CTAANGATCC CAGTGTGGTG GTACGGGAAT
            781 TCCTGCANGC CTCGAGGGCC GGCGCGCCGC GGCCGCTACG TAAATTCCGC CCCCCCCCCC
            841 CCCCTCTNCN NNCCCCCCCC CNNNNCGTTN NNTGNNNNAN NNNGCTTNNN NNAAGGNCGG
            901 GNGTGNGTTT GNCTATATGN TNATTTNNNN NNNTATTNNC NNNCNTTTTG NCANNGNGNN
            961 NNNNNNNGNA ANCNNGNNCC TGNNTNNNTN GNNNANNANT NNNNGGGGNN NTTTCCCNNN
          1021 NCNNNNNNNN GNANNGNANN NNNNNNNTNN NNNNNNNN
          //
          Primer: EGFP-N (Pr0027)
          Region: pol,gag
          Sequence file: RDB14600_A7Bak.seq check
          >D04280A1_A7Ba_1_EGFP-N_E04_13_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN TTGCTNNNNN TGGTGGCGGC GGGNGANCCG
             61 GCAGTCTAGA GGATGGTCCA CCCCCGGGGT CGGCAGCCTT CACGTGGGCG GCGTGTATCC
            121 AAGCTGCGAT GCCGTCTACT TTGAGGGCGG TGGGGGTGGT CAGCAGGACT GTGTAAGGTC
            181 CTTTCCAGCG AGGTTCTAGG TTCTTAGTCT GGTGTCGGCG GACCCACACT GTGTCGCCGA
            241 CTCGGTAAGG GTGAGGTACC ACCGGTCGGT CCAGTTGTTC TTGGTAGGCT GCCGCCAGAG
            301 GTCTCCAGAC TTCGTGCTGG ACTAAGTAGA GAGCCTGTAA GTGAGCTTGG AGAGAGGGGC
            361 TGTTAGTAAC TCTTGTCATG TCAGGGTCAG GGAAGTTTAC AAGGGGCGGG GGTGCCCCAT
            421 ATAAGATCTC ATATGGCCAT ATGGGGGCGC CTAGAGAAGG AGTGAGGGCT GGATAAAGGG
            481 AGGATCGAGG CGGGGTCGAA CGAGGAGGTT CAAGGGGGAG AGACGGGGCG GATGGAGGAA
            541 GAGGAGGCGG AGGCTTAGGG TGTACAAAGG GCTTGACCCA GGGAGGGGGG TCAAAAGCCA
            601 AGGCTTCCCA GGTCACGATG TAGGGGACCT GGTCTGGGTG TCCATGCGGG CCAGGTGAAA
            661 AGACCTTGAT CTTAACCTGG GTGATGAGGT CTCGGTTAAA GGTGCCGTCT CGCGGCCATC
            721 CGACGTTAAA GGTTGGNCAT TCTGCANAGC ANAANGNAAC CCAACGTCTC TTCTTGACNT
            781 CTACCGACTG GTTGTGAGCG ATCCGCTCNA CATCTTTCCN GNGANCTAAN GNCAAACTTA
            841 AGGGANTGNN AACNGTCTGN NCCNGGCCCA TATTTTNNNA NAANTACAGA AANNNNGNCN
            901 GANNNAGACA NNNCNGAANN ATGCTGNNNC ANANAAGANN NNCGGNNCGN NTTCNGNNCN
            961 NANNNNAAAG CNAAAANNTC NNANNNANNN NGGANNNGNT TNNNGNTCTN NNNNNCNNNC
          1021 NNNANNNNNN NNNN
          //
          Primer: EGFP-N_R2 (Pr0905)
          Region: insert mid(EGFP 5'),pol
          Sequence file: RDB14600_A7Bal.seq check
          >14600_14600_B2FhF_EGFP-N_R2_A06_03_ABI24.ab1
              1 ANNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNC NCNCCNNNGN CANGGNGGTC
             61 ACGAGGGTGG GCCAGGGCAC GGGCAGCTTG CCGGTGGTGC AGATGAACTT CAGGGTCAGC
            121 TTGCCGTAGG TGGCATCGCC CTCGCCCTCG CCGGACACGC TGAACTTGTG GCCGTTTACG
            181 TCGCCGTCCA GCTCGACCAG GATGGGCACC ACCCCGGTGA ACAGCTCCTC GCCCTTGCTC
            241 ACCATGGTGG CGGCGGGAGA TCCGGCAGTC TAGAGGATGG TCCACCCCCG GGGTCGGCAG
            301 CCTTCACGTG GGCGGCGTGT ATCCAAGCTG CGATGCCGTC TACTTTGAGG GCGGTGGGGG
            361 TGGTCAGCAG GACTGTGTAA GGTCCTTTCC AGCGAGGTTC TAGGTTCTTA GTCTGGTGTC
            421 GGCGGACCCA CACTGTGTCG CCNACTCGGT AAGGGTGAGG TACCACCGGT CGGTCCAGTT
            481 GTTCTTGGTA GGCTGCCGCC AGAGGTCTCC AGACTTCGTG CTGGACTAAG TAGAGAGCCT
            541 GTAAGTGAGC TTGGAGAGAG GGGCTGTTAG TAACTCTTGT CATGTCAGGG TCAGGGAAGT
            601 TTACAAGGGG CGGGGGTGCC CCATATAAGA TCTCATATGG NCNNNTGGGG GCGCCTAGAG
            661 AAGGAGTGAG GGCTGGATAA ANNGGAGGAT CGAGGCGGGG TCGAACGAGG NNGTTCAAGG
            721 GGGANAGACG GNNNNNATGG AGGAANNANG AGGCGGANGC TTAGGGTGNN NNNNNGGGCT
            781 TGACCCCANG GAGGGGGGNC AAAAGCNNAN NGCTTCCNNN NTCACNATGT NNGGGNNNNN
          841 NNNNN
          //
          Primer: mCherry-C_R (Pr0517)
          Region: insert mid(mRFP1 5',rat LC3 3')
          Sequence file: RDB14600_A7Bam.seq check
          >14600_14600_B2FhF_mCherry-C_R_D06_12_ABI24.ab1
              1 NNNNNNNNNN NNNNNCNGCN NNNNGTCGGT CTTGTAGGCG CCGGGCAGCT GCACGGGCTT
             61 CTTGGCCNTG TANGNGGTCT TGACCTCGGC GTCGTAGTGG CCGCCGTCCT TCAGCTTCAG
            121 CCTCATCTTG ATCTCGCCCT TCAGGGCGCC GTCCTCGGGG TACATCCGCT CGGTGGAGGC
            181 CTCCCAGCCC ATGGTCTTCT TCTGCATTAC GGGGCCGTCG GAGGGGAAGT TGGTGCCGCG
            241 CAGCTTCACC TTGTAGATGA ACTCGCCGTC CTGCAGGGAG GAGTCCTGGG TCACGGTCAC
            301 CACGCCGCCG TCCTCGAAGT TCATCACGCG CTCCCACTTG AAGCCCTCGG GGAAGGACAG
            361 CTTCAAGTAG TCGGGGATGT CGGCGGGGTG CTTCACGTAG GCCTTGGAGC CGTACTGGAA
            421 CTGAGGGGAC AGGATGTCCC AGGCGAAGGG CAGGGGGCCG CCCTTGGTCA CCTTCAGCTT
            481 GGCGGTCTGG GTGCCCTCGT AGGGGCGGCC CTCGCCCTCG CCCTCGATCT CGAACTCGTG
            541 GCCGTTCACG GAGCCCTCCA TGCGCACCTT GAAGCGCATG AACTCCTTGA TGACGTCCTC
            601 GGAGGAGGCC ATCCCGAACG TCTCCTGGGA GGCATAGACC ATGTACAGGA AGCCGTCTTC
            661 ATCTCTCTCG CTCTCGTACA CTTCAGAGAT GGGTGTGGAC ACACTCACCA TGCTGTGCCC
            721 ATTCACCAGG AGGAAGAAGG NTTGGTTAGC ATTGAGCTGC AGGCGCCTTC TAATTATCTT
            781 GATGAGTTCG CTCATATTCA CGTGATCAGG TACAAGGAAC TTGGTCTTGT CCAGGANGGG
            841 CAGCTGCTTC TCACCCTTGT ATCGCTCTAT AATCACTGGG ATCTTGGTGG GGTGCTGCTC
            901 CCGGATGANC CGGANATCTN NCACTCTTTG TNCNAAGCTC CNGNGCTGTT TGNANGNCTT
            961 CTCNNANNGN NNNGNNATTN NANTCNTCNN TAGATNCGNN GNNNCCNNAN NNNNNCCGNC
          1021 CNNNNNNNNG NNNCCCNTNN NNNNNNNN
          //
          Primer: pMXs-3LTRup_R2 (Pr0365)
          Region: PuroR,IRES
          Sequence file: RDB14600_A7Ban.seq check
          >D04280A1_A7Ba_1_pMXs-3LTRup_R2_G04_19_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNT TNNTCGTCGA GCCNTGGGGT CGTGCGCTCC TTTCGGTCGG
             61 GCGCTGCGGG TCGTGGGGCG GGCGTCAGGC ACCGGGCTTG CGGGTCATGC ACCAGGTGCG
            121 CGGTCCTTCG GGCACCTCGA CGTCGGCGGT GACGGTGAAG CCGAGCCGCT CGTAGAAGGG
            181 GAGGTTGCGG GGCGCGGAGG TCTCCAGGAA GGCGGGCACC CCGGCGCGCT CGGCCGCCTC
            241 CACTCCGGGG AGCACGACGG CGCTGCCCAG ACCCTTGCCC TGGTGGTCGG GCGAGACGCC
            301 GACGGTGGCC AGGAACCACG CGGGCTCCTT GGGCCGGTGC GGCGCCAGGA GGCCTTCCAT
            361 CTGTTGCTGC GCGGCCAGCC GGGAACCGCT CAACTCGGCC ATGCGCGGGC CGATCTCGGC
            421 GAACACCGCC CCCGCTTCGA CGCTCTCCGG CGTGGTCCAG ACCGCCACCG CGGCGCCGTC
            481 GTCCGCGACC CACACCTTGC CGATGTCGAG CCCGACGCGC GTGAGGAAGA GTTCTTGCAG
            541 CTCGGTGACC CGCTCGATGT GGCGGTCCGG GTCGACGGTG TGGCGCGTGG CGGGGTAGTC
            601 GGCGAACGCG GCGGCGAGGG TGCGTACGGC CCGGGGGACG TCGTCGCGGG TGGCGAGGCG
            661 CACCGTGGGC TTGTACTCGG TCATGGTTGT GGCCATATTA TCATCGTGTT TTTCAAAGGA
            721 AAACCACGTC NNCGTGGTTC GGGGGGCCTA GACGTTTTTT TAACCTCGAC TAAACACATG
            781 TAAAGCATGT GCACCGAGGC CCCAGATCAN ATCCCNTACN ATGGGGTACC TTCTGNNCAT
            841 CCTTCNGCCC CTTGNTGAAT ACNCTNGANG ANAGNCNTTT GANTCNTTNC NCNNCTATCC
            901 NACTCNCANC GNGGNACNNG GGNNGNGNCG CNTTNNCNNN NGNNNCTTAT ACNCNNGNNT
          961 TTTNGNNNNC NNANNNNNCN GNNGNNNNGN NGGGGGGNNN NNNNNNN
          //
          Primer: p15Aori_F (Pr0276)
          Region: ori,LTR
          Sequence file: RDB14600_A7Bao.seq check
          >D04280A1_A7Ba_1_p15Aori_F_F04_16_ABI24.ab1
              1 NNNNNNNNNN NNNNTTTGNG NNGNTCGTCN GGGGGGCGGA NCCTATGGAA AAACGCCAGC
             61 AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT
            121 GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT
            181 CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA
            241 ATACGCAAAC CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAACTA TGGCCATTTA
            301 ATGTAAATAC TTAAGAAAAA AAACCAAATT AATTTTGATA CATGCTGCAT GTGAAAGACC
            361 CCCGCTGACG GGTAGTCAAT CACTCAGAGG AGACCCTCCC AAGGAACAGC GAGACCACAA
            421 GTCGGATGCA ACTGCAAGAG GGTTTATTGG ATACACGGGT ACCCGGGCGA CTCAGTCAAT
            481 CGGAGGACTG GCGCCCCGAG TGAGGGGTTG TGGGCTCTTT TATTGAGCTC GGGGAGCAGA
            541 AGCGCGCGAA CAGAAGCGAG AAGCGAACTG ATTGGTTAGT TCAAATAAGG CACAGGGTCA
            601 TTTCAGGTCC TTGGGGCACC CTGGAAACAT CTGATGGTTC TCTAGAAACT GCTGANGGCT
            661 GGACCGCATC TGGGGANCAT CTGTTCTTGN NCCTGANCCG GGGCAGGAAC TGCTTANCAC
            721 AGATATCCTG TTTGGNCCAT ATTCNGCTGT TCCATCTGTT CTTGGNCCTG ANCCGGGGCA
            781 NGANCTGCTT ACCNCAGATA TCCTGNTTGG NCCATATTCN GCTGTNCCNT CTGNTCCTGA
            841 NCTTGATCTG AACTTCTCTA TTCTCAGTTA TGTATTTTTC CNTGCCTNGN AAAATGGCGT
            901 TACTTAANCT AGNTTGNCNA NNNTACNNNN GGGGNNNTNC NTTCCNNCCT TTTTCNGNAN
            961 ANTAAANAAA ATCNTTTANT TNATCNNCNA NNNNNGGGGN CNNGNNNNNN NTTNNNNNNG
          1021 NNNNNNNNGN NNNNNNNNN
          //
          Primer: MMLV psi_R1 (Pr1054)
          Region: MMLV psi,CMV en
          Sequence file: RDB14600_A7Bap.seq check
          >14600_14600_B2FhF_MMLV psi_R1_C06_09_ABI24.ab1
              1 NNNNNNNNNN NNNNNANCNN GNNNGNNNCN GATAAGTTGC TGGCCNGCTT ACCTCCCGGT
             61 GGTGGGTCGG TGGTCCCTGG GCAGGGGTCT CCCGATCCCG GACGAGCCCC CAAATGAAAG
            121 ACCCCCGCTG ACGGGTAGTC AATCACTCAG AGGAGACCCT CCCAAGGAAC AGCGAGACCA
            181 CAAGTCGGAT GCAACTGCAA GAGGGTTTAT TGGATACACG GGTACCCGGG CGACTCAGTC
            241 AATCGGAGGA CTGGCGCGCC GAGTGAGGGG TTGTGGGCTC TTTTATTGAG CTCGGGGAGC
            301 AGAAGCGCGC GAACAGAAGC GAGAAGCGAA CTGATTGGTT AGTTCAAATA AGGCACAGGG
            361 TCATTTCAGG TCCTTGGGGC ACCCTGGAAA CATCTGATGG TTCTCTAGCA TGGTAATAGC
            421 GATGACTAAT ACGTAGATGT ACTGCCAAGT AGGAAAGTCC CATAAGGTCA TGTACTGGGC
            481 ATAATGCCAG GCGGGCCATT TACCGTCATT GACGTCAATA GGGGGCGTAC TTGGCATATG
            541 ATACACTTGA TGTACTGCCA AGTGGGCAGT TTACCGTAAA TACTCCACCC ATTGACGTCA
            601 ATGGAAAGTC CCTATTGGCG TTACTATGGG AACATACGTC ATTATTGACG TCAATGGGCG
            661 GGGGTCGTTG GGCGGTCAGC CAGGCGGGCC ATTTACCGTA AGTTATGTAA CGCGGAACTC
            721 CATATATGGG CTATGAACTA ATGACCCCGT AATTGATTAC TATTAATAAC TAGTCAATAA
            781 TCAATGTCAA CATGGCGGTA ATGTTGGACA TGANCCAATA TAAATGTACA TATTATGATA
            841 TGGATACNAC GTATGCAATG GGCCNAGCTC CTCGAGTGAA GANGAAANGG CCTCGNGATA
            901 CGCCNATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTANACGTC NNGNGGNNNT
            961 TTTCNGGGAA ATGTGCNNNG ANNCCCNANT TGNTNATTTT NCTAANNACN TNNNANNNNG
          1021 TANNCNNCNN NNNANNNNNN NNNCNNNNNN NNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Morishita, H., A new probe to measure autophagic flux in vitro and in vivo. Autophagy 13 (4): 757-758 (2017). PMID 28121224.
          original Kaizuka, T., An Autophagic Flux Probe that Releases an Internal Control. Mol Cell. 2016 Nov 17;64(4):835-849 (2016). PMID 27818143.
          reference Nguyen, T.B., DGAT1-Dependent Lipid Droplet Biogenesis Protects Mitochondrial Function during Starvation-Induced Autophagy. Dev. Cell 42 (1): 9-21 (2017). PMID 28697336.
          user_report Hirose, S., NRF3 activates mTORC1 arginine-dependently for cancer cell viability. iScience 26 (2): 106045 (2023). PMID 36818298.
          user_report Ohnishi, K., Identification of Dietary Phytochemicals Capable of Enhancing the Autophagy Flux in HeLa and Caco-2 Human Cell Lines. Antioxidants (Basel) 9 (12): 1193 (2020). PMID 33261065.

          2023.05.22

          GNP_filter3_RDBDEP_html_230512.pl