RDB14600 - pMRX-IP-GFP-LC3-RFP-LC3 delta G

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pMRX-IP-GFP-LC3-RFP-LC3 delta G (#RDB14600)

Fluorescent probe to evaluate autophagic flux by the rat LC3 fused with GFP and RFP.

Clone info.	Fluorescent probe to evaluate autophagic flux. Expression vector of rat microtubule-associated protein 1 light chain 3 beta (LC3) fused with GFP and RFP. This probe is cleaved by endogenous ATG4 proteases into equimolar amounts of GFP-LC3 and RFP-LC3deltaG. The GFP-LC3 is degraded by autophagy, while RFP-LC3deltaG remains in the cytosol as an internal control. Thus, autophagic flux can be estimated by calculating the GFP/RFP signal ratio. Please refer DISCUSSION section of Kaizuka, T., Mol. Cell 64, 835-849, 2016 for the difference between pMRX-IP-GFP-LC3-RFP and this clone.
Comment	Expression was confirmed by the depositor with immunoblotting and fluorescent microscopy. Please refer Saitoh, T. et al., FEBS Lett., 532 (1-2): 45-51, (2002) for the construction of pMRX-IP vector.
Vector backbone	pMRX-IP (Plasmid)
Selectable markers	Amp^r (E. coli), Puro^r (mammalian cells).
Growth conditions	LB+Amp, 37oC
Gene/insert name	Rat LC3 cDNA Aequorea victoria GFP cDNA Discosoma sp. RFP cDNA
Depositor\|Developer	Mizushima, Noboru \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Approval form by depositor [Word]
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution (1) BIOLOGICAL RESOURCE is limited to for academic research in non-profit organization. (2) The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. (3) Any use of the BIOLOGICAL RESOURCE for profit purposes by a non-profit organization or any use of the BIOLOGICAL RESOURCE by a profit organization requires a separate license from the DEPOSITOR prior to distribution. (4) In publishing research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the original publication designated by the DEPOSITOR (Kaizuka, T. et al. Mol. Cell, 64 (4): 835-849, 2016) and an acknowledgment to the DEPOSITOR are requested. (5) The RECIPIENT must contact the DEPOSITOR in case of application for any patents or commercial use with the results from the use of BIOLOGICAL RESOURCE.
Additional terms and conditions:
Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.

Remarks ((Additional form)) Approval Form(FormD)
Remember that you will be working with samples containing infectious virus.
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
Please obtain written approval of the depositor.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Approval form by depositor [Word] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	(1) BIOLOGICAL RESOURCE is limited to for academic research in non-profit organization. (2) The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. (3) Any use of the BIOLOGICAL RESOURCE for profit purposes by a non-profit organization or any use of the BIOLOGICAL RESOURCE by a profit organization requires a separate license from the DEPOSITOR prior to distribution. (4) In publishing research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the original publication designated by the DEPOSITOR (Kaizuka, T. et al. Mol. Cell, 64 (4): 835-849, 2016) and an acknowledgment to the DEPOSITOR are requested. (5) The RECIPIENT must contact the DEPOSITOR in case of application for any patents or commercial use with the results from the use of BIOLOGICAL RESOURCE. Additional terms and conditions: Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
Remarks	((Additional form)) Approval Form(FormD) Remember that you will be working with samples containing infectious virus. This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. Please obtain written approval of the depositor.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 提供同意書 (MTA, 営利目的用)[Word] 寄託者による提供承諾書 [Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	1. 非営利団体による学術研究に限る。2. 利用者は事前に寄託者の提供承諾書を得る。3. 当該リソースの非営利団体の営利目的での利用および営利団体の利用については、事前に寄託者との間で、別途使用許諾に係る契約の締結が必要である。4. 研究成果の公表にあたって寄託者の指定する文献(Kaizuka, T. et al. Mol. Cell, 64 (4): 835-849, 2016)を引用し、謝辞の表明を必要とする。5. 利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。付加的使用条件： pMRXベクターについて：利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献（Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
備考	【追加提供依頼書】承諾書(FormD) このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。寄託者の書面による提供承諾が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB14600	pMRX-IP-GFP-LC3-RFP-LC3 delta G	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMRX-IP-GFP-LC3-RFP-LC3 delta G was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14600).

Reference section:

Morishita, H., Kaizuka, T., Hama, Y., Mizushima, N., A new probe to measure autophagic flux in vitro and in vivo. Autophagy 13 (4): 757-758 (2017). PMID 28121224. [link to RRC of NBRP]

Kaizuka, T., Morishita, H., Hama, Y., Tsukamoto, S., Matsui, T., Toyota, Y., Kodama, A., Ishihara, T., Mizushima, T., Mizushima, N., An Autophagic Flux Probe that Releases an Internal Control. Mol Cell. 2016 Nov 17;64(4):835-849 (2016). PMID 27818143. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB14600_A7Bap1-5.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F2 (Pr0364) Region: insert 5'(EGFP 5') Sequence file: RDB14600_A7Bag.seq
>D04280A1_A7Ba_1_MMLV-pol_F2_C04_07_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNCC NCNNNNNTGC CGGNNCTCCC GCCGCCNCCA TGGTGAGCAA 61 GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA 121 CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC 181 CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC 241 CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC AGCACGACTT 301 CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA 361 CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT 421 CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA 481 CAACTACAAC AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG GCATCAAGGT 541 GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG ACCACTACCA 601 GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCAC 661 CCAGTCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT 721 CGTGACCGCC GCCGGGATCA CTCTCGGCAT GGACGANCTG TACAAGGNTG GCGGTGGCTC 781 GNGCGGTGGT GNGTCGGGTG GCGGCGGNTC TATCGATGAA TTGAATTCCN TGCCGTCNGA 841 NAAGANCTTC NAACAGCGCC NGANCTNCNA ACAAAGANTG GAANANGNCC GGNTNNTNCN 901 GGNNCANCAC CCCNCCAANA TCCNNGNGNN TATANANCGA TACNNGGNNG ANAANCANCT 961 GCCNNNCNNN NNNNANNNNN TTNNNNGNNN NNGNNNNCGT NNNNNTGANC NNNNTCNTCN 1021 NNANNANTNN NNNNNNNN //
Primer: mRFP1-C_F (Pr0502) Region: insert 3'(rat LC3 deltaG),IRES Sequence file: RDB14600_A7Bah.seq
>14600_14600_B2FhF_1_mRFP1-C_F_B01_02_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NCTGGNNNTC NCCTCCCNCA ACGAGGACTA CACCATCGTG 61 GAACAGTACG AGCNCNCCNA GGGCCGCCAC TCCACCGGCG CCATCGATGA ATTGAATTCC 121 ATGCCGTCCG AGNNNNNNNN NN //
Primer: mVenus-M_F (Pr0574) Region: insert mid(EGFP 3',rat LC3,mRFP1 5') Sequence file: RDB14600_A7Bai.seq
>14600_14600_B2FhF_mVenus-M_F_B06_06_ABI24.ab1 1 NNNNNNNNNN NNNNNGGCAT CNNGGTGNAC TTNNAGANCC GCCNCAACAT CGAGGACGGC 61 AGCGTGCAGC TCGCCGACCA CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG 121 CTGCCCGACA ACCACTACCT GAGCACCCAG TCCGCCCTGA GCAAAGACCC CAACGAGAAG 181 CGCGATCACA TGGTCCTGCT GGAGTTCGTG ACCGCCGCCG GGATCACTCT CGGCATGGAC 241 GAGCTGTACA AGGGTGGCGG TGGCTCGGGC GGTGGTGGGT CGGGTGGCGG CGGATCTATC 301 GATGAATTGA ATTCCATGCC GTCCGAGAAG ACCTTCAAAC AGCGCCGGAG CTTCGAACAA 361 AGAGTGGAAG ATGTCCGGCT CATCCGGGAG CAGCACCCCA CCAAGATCCC AGTGATTATA 421 GAGCGATACA AGGGTGAGAA GCAGCTGCCC GTCCTGGACA AGACCAAGTT CCTTGTACCT 481 GATCACGTGA ATATGAGCGA ACTCATCAAG ATAATTAGAA GGCGCCTGCA GCTCAATGCT 541 AACCAAGCCT TCTTCCTCCT GGTGAATGGG CACAGCATGG TGAGTGTGTC CACACCCATC 601 TCTGAAGTGT ACGAGAGCGA GAGAGATGAA GACGGCTTCC TGTACATGGT CTATGCCTCC 661 CAGGAGACGT TCGGGATGGC CTCCTCCGAG GANGTCATCA AGGAGTTCAT GCGCTTCAAG 721 GTGCGCATGG ANGGCTCCGT GAACGGNCAC GAGTTCGAGA TCGANGGNGA NGGCGANGGN 781 CGCCCCTACG AGGGCACCCA GACCGCCNAG CTGAANGTGA NCAANGGCGG NCCCCTGCCC 841 TTCNCCNGGG ACATCCNGNC CCCTCNGTTC CAGTACNGNT CCNAGGNCTA CGTGAAGCAN 901 CCCGCCNANN TCCCNNANNA CTTGAAGCTG TCCTTCCCCG ANGGCTTCNA GNNNNNNCNC 961 GNGNTGAACT TCGANGANNN NNNCNNNNNG ANNNNNNNCC NNNNTNNTNC CNGNNNNNNN 1021 NNN //
Primer: mRFP1-inner1 (Pr0443) Region: insert mid(mRFP1 3',rat LC3 deltaG 5') Sequence file: RDB14600_A7Baj.seq
>D04280A1_A7Ba_1_mRFP1-inner1_E10_13_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNCNNNNN NTCCTCCCTG NNGGNNGGCG AGTTCATCTA 61 CAAGGTGAAG CTGCGCGGCA CCAACTTCCC CTCCGACGGC CCCGTAATGC AGAAGAAGAC 121 CATGGGCTGG GAGGCCTCCA CCGAGCGGAT GTACCCCGAG GACGGCGCCC TGAAGGGCGA 181 GATCAAGATG AGGCTGAAGC TGAAGGACGG CGGCCACTAC GACGCCGAGG TCAAGACCAC 241 CTACATGGCC AAGAAGCCCG TGCAGCTGCC CGGCGCCTAC AAGACCGACA TCAAGCTGGA 301 CATCACCTCC CACAACGAGG ACTACACCAT CGTGGAACAG TACGAGCGCG CCGAGGGCCG 361 CCACTCCACC GGCGCCATCG ATGAATTGAA TTCCATGCCG TCCGAGAAGA CCTTCAAACA 421 GCGCCGGAGC TTCGAACAAA GAGTGGAAGA TGTCCGGCTC ATCCGGGAGC AGCACCCCAC 481 CAAGATCCCA GTGATTATAG AGCGATACAA GGGTGAGAAG CAGCTGCCCG TCCTGGACAA 541 GACCAAGTTC CTTGTACCTG ATCACGTGAA TATGAGCGAA CTCATCAAGA TAATTAGAAG 601 GCGCCTGCAG CTCAATGCTA ACCAAGCCTT CTTCCTCCTG GTGAATGGGC ACAGCATGGT 661 GAGTGTGTCC ACACCCATCT CTGAAGTGTA CGAGAGCGAG AGAGATGAAG ACGGCTTCCT 721 GTACATGGTC TATGCCTCCC AGGANACGTT CTAANGATCC CAGTGTGGTG GTACGGGAAT 781 TCCTGCANGC CTCGAGGGCC GGCGCGCCGC GGCCGCTACG TAAATTCCGC CCCCCCCCCC 841 CCCCTCTNCN NNCCCCCCCC CNNNNCGTTN NNTGNNNNAN NNNGCTTNNN NNAAGGNCGG 901 GNGTGNGTTT GNCTATATGN TNATTTNNNN NNNTATTNNC NNNCNTTTTG NCANNGNGNN 961 NNNNNNNGNA ANCNNGNNCC TGNNTNNNTN GNNNANNANT NNNNGGGGNN NTTTCCCNNN 1021 NCNNNNNNNN GNANNGNANN NNNNNNNTNN NNNNNNNN //
Primer: EGFP-N (Pr0027) Region: pol,gag Sequence file: RDB14600_A7Bak.seq
>D04280A1_A7Ba_1_EGFP-N_E04_13_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN TTGCTNNNNN TGGTGGCGGC GGGNGANCCG 61 GCAGTCTAGA GGATGGTCCA CCCCCGGGGT CGGCAGCCTT CACGTGGGCG GCGTGTATCC 121 AAGCTGCGAT GCCGTCTACT TTGAGGGCGG TGGGGGTGGT CAGCAGGACT GTGTAAGGTC 181 CTTTCCAGCG AGGTTCTAGG TTCTTAGTCT GGTGTCGGCG GACCCACACT GTGTCGCCGA 241 CTCGGTAAGG GTGAGGTACC ACCGGTCGGT CCAGTTGTTC TTGGTAGGCT GCCGCCAGAG 301 GTCTCCAGAC TTCGTGCTGG ACTAAGTAGA GAGCCTGTAA GTGAGCTTGG AGAGAGGGGC 361 TGTTAGTAAC TCTTGTCATG TCAGGGTCAG GGAAGTTTAC AAGGGGCGGG GGTGCCCCAT 421 ATAAGATCTC ATATGGCCAT ATGGGGGCGC CTAGAGAAGG AGTGAGGGCT GGATAAAGGG 481 AGGATCGAGG CGGGGTCGAA CGAGGAGGTT CAAGGGGGAG AGACGGGGCG GATGGAGGAA 541 GAGGAGGCGG AGGCTTAGGG TGTACAAAGG GCTTGACCCA GGGAGGGGGG TCAAAAGCCA 601 AGGCTTCCCA GGTCACGATG TAGGGGACCT GGTCTGGGTG TCCATGCGGG CCAGGTGAAA 661 AGACCTTGAT CTTAACCTGG GTGATGAGGT CTCGGTTAAA GGTGCCGTCT CGCGGCCATC 721 CGACGTTAAA GGTTGGNCAT TCTGCANAGC ANAANGNAAC CCAACGTCTC TTCTTGACNT 781 CTACCGACTG GTTGTGAGCG ATCCGCTCNA CATCTTTCCN GNGANCTAAN GNCAAACTTA 841 AGGGANTGNN AACNGTCTGN NCCNGGCCCA TATTTTNNNA NAANTACAGA AANNNNGNCN 901 GANNNAGACA NNNCNGAANN ATGCTGNNNC ANANAAGANN NNCGGNNCGN NTTCNGNNCN 961 NANNNNAAAG CNAAAANNTC NNANNNANNN NGGANNNGNT TNNNGNTCTN NNNNNCNNNC 1021 NNNANNNNNN NNNN //
Primer: EGFP-N_R2 (Pr0905) Region: insert mid(EGFP 5'),pol Sequence file: RDB14600_A7Bal.seq
>14600_14600_B2FhF_EGFP-N_R2_A06_03_ABI24.ab1 1 ANNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNC NCNCCNNNGN CANGGNGGTC 61 ACGAGGGTGG GCCAGGGCAC GGGCAGCTTG CCGGTGGTGC AGATGAACTT CAGGGTCAGC 121 TTGCCGTAGG TGGCATCGCC CTCGCCCTCG CCGGACACGC TGAACTTGTG GCCGTTTACG 181 TCGCCGTCCA GCTCGACCAG GATGGGCACC ACCCCGGTGA ACAGCTCCTC GCCCTTGCTC 241 ACCATGGTGG CGGCGGGAGA TCCGGCAGTC TAGAGGATGG TCCACCCCCG GGGTCGGCAG 301 CCTTCACGTG GGCGGCGTGT ATCCAAGCTG CGATGCCGTC TACTTTGAGG GCGGTGGGGG 361 TGGTCAGCAG GACTGTGTAA GGTCCTTTCC AGCGAGGTTC TAGGTTCTTA GTCTGGTGTC 421 GGCGGACCCA CACTGTGTCG CCNACTCGGT AAGGGTGAGG TACCACCGGT CGGTCCAGTT 481 GTTCTTGGTA GGCTGCCGCC AGAGGTCTCC AGACTTCGTG CTGGACTAAG TAGAGAGCCT 541 GTAAGTGAGC TTGGAGAGAG GGGCTGTTAG TAACTCTTGT CATGTCAGGG TCAGGGAAGT 601 TTACAAGGGG CGGGGGTGCC CCATATAAGA TCTCATATGG NCNNNTGGGG GCGCCTAGAG 661 AAGGAGTGAG GGCTGGATAA ANNGGAGGAT CGAGGCGGGG TCGAACGAGG NNGTTCAAGG 721 GGGANAGACG GNNNNNATGG AGGAANNANG AGGCGGANGC TTAGGGTGNN NNNNNGGGCT 781 TGACCCCANG GAGGGGGGNC AAAAGCNNAN NGCTTCCNNN NTCACNATGT NNGGGNNNNN 841 NNNNN //
Primer: mCherry-C_R (Pr0517) Region: insert mid(mRFP1 5',rat LC3 3') Sequence file: RDB14600_A7Bam.seq
>14600_14600_B2FhF_mCherry-C_R_D06_12_ABI24.ab1 1 NNNNNNNNNN NNNNNCNGCN NNNNGTCGGT CTTGTAGGCG CCGGGCAGCT GCACGGGCTT 61 CTTGGCCNTG TANGNGGTCT TGACCTCGGC GTCGTAGTGG CCGCCGTCCT TCAGCTTCAG 121 CCTCATCTTG ATCTCGCCCT TCAGGGCGCC GTCCTCGGGG TACATCCGCT CGGTGGAGGC 181 CTCCCAGCCC ATGGTCTTCT TCTGCATTAC GGGGCCGTCG GAGGGGAAGT TGGTGCCGCG 241 CAGCTTCACC TTGTAGATGA ACTCGCCGTC CTGCAGGGAG GAGTCCTGGG TCACGGTCAC 301 CACGCCGCCG TCCTCGAAGT TCATCACGCG CTCCCACTTG AAGCCCTCGG GGAAGGACAG 361 CTTCAAGTAG TCGGGGATGT CGGCGGGGTG CTTCACGTAG GCCTTGGAGC CGTACTGGAA 421 CTGAGGGGAC AGGATGTCCC AGGCGAAGGG CAGGGGGCCG CCCTTGGTCA CCTTCAGCTT 481 GGCGGTCTGG GTGCCCTCGT AGGGGCGGCC CTCGCCCTCG CCCTCGATCT CGAACTCGTG 541 GCCGTTCACG GAGCCCTCCA TGCGCACCTT GAAGCGCATG AACTCCTTGA TGACGTCCTC 601 GGAGGAGGCC ATCCCGAACG TCTCCTGGGA GGCATAGACC ATGTACAGGA AGCCGTCTTC 661 ATCTCTCTCG CTCTCGTACA CTTCAGAGAT GGGTGTGGAC ACACTCACCA TGCTGTGCCC 721 ATTCACCAGG AGGAAGAAGG NTTGGTTAGC ATTGAGCTGC AGGCGCCTTC TAATTATCTT 781 GATGAGTTCG CTCATATTCA CGTGATCAGG TACAAGGAAC TTGGTCTTGT CCAGGANGGG 841 CAGCTGCTTC TCACCCTTGT ATCGCTCTAT AATCACTGGG ATCTTGGTGG GGTGCTGCTC 901 CCGGATGANC CGGANATCTN NCACTCTTTG TNCNAAGCTC CNGNGCTGTT TGNANGNCTT 961 CTCNNANNGN NNNGNNATTN NANTCNTCNN TAGATNCGNN GNNNCCNNAN NNNNNCCGNC 1021 CNNNNNNNNG NNNCCCNTNN NNNNNNNN //
Primer: pMXs-3LTRup_R2 (Pr0365) Region: PuroR,IRES Sequence file: RDB14600_A7Ban.seq
>D04280A1_A7Ba_1_pMXs-3LTRup_R2_G04_19_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNT TNNTCGTCGA GCCNTGGGGT CGTGCGCTCC TTTCGGTCGG 61 GCGCTGCGGG TCGTGGGGCG GGCGTCAGGC ACCGGGCTTG CGGGTCATGC ACCAGGTGCG 121 CGGTCCTTCG GGCACCTCGA CGTCGGCGGT GACGGTGAAG CCGAGCCGCT CGTAGAAGGG 181 GAGGTTGCGG GGCGCGGAGG TCTCCAGGAA GGCGGGCACC CCGGCGCGCT CGGCCGCCTC 241 CACTCCGGGG AGCACGACGG CGCTGCCCAG ACCCTTGCCC TGGTGGTCGG GCGAGACGCC 301 GACGGTGGCC AGGAACCACG CGGGCTCCTT GGGCCGGTGC GGCGCCAGGA GGCCTTCCAT 361 CTGTTGCTGC GCGGCCAGCC GGGAACCGCT CAACTCGGCC ATGCGCGGGC CGATCTCGGC 421 GAACACCGCC CCCGCTTCGA CGCTCTCCGG CGTGGTCCAG ACCGCCACCG CGGCGCCGTC 481 GTCCGCGACC CACACCTTGC CGATGTCGAG CCCGACGCGC GTGAGGAAGA GTTCTTGCAG 541 CTCGGTGACC CGCTCGATGT GGCGGTCCGG GTCGACGGTG TGGCGCGTGG CGGGGTAGTC 601 GGCGAACGCG GCGGCGAGGG TGCGTACGGC CCGGGGGACG TCGTCGCGGG TGGCGAGGCG 661 CACCGTGGGC TTGTACTCGG TCATGGTTGT GGCCATATTA TCATCGTGTT TTTCAAAGGA 721 AAACCACGTC NNCGTGGTTC GGGGGGCCTA GACGTTTTTT TAACCTCGAC TAAACACATG 781 TAAAGCATGT GCACCGAGGC CCCAGATCAN ATCCCNTACN ATGGGGTACC TTCTGNNCAT 841 CCTTCNGCCC CTTGNTGAAT ACNCTNGANG ANAGNCNTTT GANTCNTTNC NCNNCTATCC 901 NACTCNCANC GNGGNACNNG GGNNGNGNCG CNTTNNCNNN NGNNNCTTAT ACNCNNGNNT 961 TTTNGNNNNC NNANNNNNCN GNNGNNNNGN NGGGGGGNNN NNNNNNN //
Primer: p15Aori_F (Pr0276) Region: ori,LTR Sequence file: RDB14600_A7Bao.seq
>D04280A1_A7Ba_1_p15Aori_F_F04_16_ABI24.ab1 1 NNNNNNNNNN NNNNTTTGNG NNGNTCGTCN GGGGGGCGGA NCCTATGGAA AAACGCCAGC 61 AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT 121 GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT 181 CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA 241 ATACGCAAAC CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAACTA TGGCCATTTA 301 ATGTAAATAC TTAAGAAAAA AAACCAAATT AATTTTGATA CATGCTGCAT GTGAAAGACC 361 CCCGCTGACG GGTAGTCAAT CACTCAGAGG AGACCCTCCC AAGGAACAGC GAGACCACAA 421 GTCGGATGCA ACTGCAAGAG GGTTTATTGG ATACACGGGT ACCCGGGCGA CTCAGTCAAT 481 CGGAGGACTG GCGCCCCGAG TGAGGGGTTG TGGGCTCTTT TATTGAGCTC GGGGAGCAGA 541 AGCGCGCGAA CAGAAGCGAG AAGCGAACTG ATTGGTTAGT TCAAATAAGG CACAGGGTCA 601 TTTCAGGTCC TTGGGGCACC CTGGAAACAT CTGATGGTTC TCTAGAAACT GCTGANGGCT 661 GGACCGCATC TGGGGANCAT CTGTTCTTGN NCCTGANCCG GGGCAGGAAC TGCTTANCAC 721 AGATATCCTG TTTGGNCCAT ATTCNGCTGT TCCATCTGTT CTTGGNCCTG ANCCGGGGCA 781 NGANCTGCTT ACCNCAGATA TCCTGNTTGG NCCATATTCN GCTGTNCCNT CTGNTCCTGA 841 NCTTGATCTG AACTTCTCTA TTCTCAGTTA TGTATTTTTC CNTGCCTNGN AAAATGGCGT 901 TACTTAANCT AGNTTGNCNA NNNTACNNNN GGGGNNNTNC NTTCCNNCCT TTTTCNGNAN 961 ANTAAANAAA ATCNTTTANT TNATCNNCNA NNNNNGGGGN CNNGNNNNNN NTTNNNNNNG 1021 NNNNNNNNGN NNNNNNNNN //
Primer: MMLV psi_R1 (Pr1054) Region: MMLV psi,CMV en Sequence file: RDB14600_A7Bap.seq
>14600_14600_B2FhF_MMLV psi_R1_C06_09_ABI24.ab1 1 NNNNNNNNNN NNNNNANCNN GNNNGNNNCN GATAAGTTGC TGGCCNGCTT ACCTCCCGGT 61 GGTGGGTCGG TGGTCCCTGG GCAGGGGTCT CCCGATCCCG GACGAGCCCC CAAATGAAAG 121 ACCCCCGCTG ACGGGTAGTC AATCACTCAG AGGAGACCCT CCCAAGGAAC AGCGAGACCA 181 CAAGTCGGAT GCAACTGCAA GAGGGTTTAT TGGATACACG GGTACCCGGG CGACTCAGTC 241 AATCGGAGGA CTGGCGCGCC GAGTGAGGGG TTGTGGGCTC TTTTATTGAG CTCGGGGAGC 301 AGAAGCGCGC GAACAGAAGC GAGAAGCGAA CTGATTGGTT AGTTCAAATA AGGCACAGGG 361 TCATTTCAGG TCCTTGGGGC ACCCTGGAAA CATCTGATGG TTCTCTAGCA TGGTAATAGC 421 GATGACTAAT ACGTAGATGT ACTGCCAAGT AGGAAAGTCC CATAAGGTCA TGTACTGGGC 481 ATAATGCCAG GCGGGCCATT TACCGTCATT GACGTCAATA GGGGGCGTAC TTGGCATATG 541 ATACACTTGA TGTACTGCCA AGTGGGCAGT TTACCGTAAA TACTCCACCC ATTGACGTCA 601 ATGGAAAGTC CCTATTGGCG TTACTATGGG AACATACGTC ATTATTGACG TCAATGGGCG 661 GGGGTCGTTG GGCGGTCAGC CAGGCGGGCC ATTTACCGTA AGTTATGTAA CGCGGAACTC 721 CATATATGGG CTATGAACTA ATGACCCCGT AATTGATTAC TATTAATAAC TAGTCAATAA 781 TCAATGTCAA CATGGCGGTA ATGTTGGACA TGANCCAATA TAAATGTACA TATTATGATA 841 TGGATACNAC GTATGCAATG GGCCNAGCTC CTCGAGTGAA GANGAAANGG CCTCGNGATA 901 CGCCNATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTANACGTC NNGNGGNNNT 961 TTTCNGGGAA ATGTGCNNNG ANNCCCNANT TGNTNATTTT NCTAANNACN TNNNANNNNG 1021 TANNCNNCNN NNNANNNNNN NNNCNNNNNN NNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Morishita, H., A new probe to measure autophagic flux in vitro and in vivo. Autophagy 13 (4): 757-758 (2017). PMID 28121224. [link to RRC of NBRP]
original	Kaizuka, T., An Autophagic Flux Probe that Releases an Internal Control. Mol Cell. 2016 Nov 17;64(4):835-849 (2016). PMID 27818143. [link to RRC of NBRP]
reference	Nguyen, T.B., DGAT1-Dependent Lipid Droplet Biogenesis Protects Mitochondrial Function during Starvation-Induced Autophagy. Dev. Cell 42 (1): 9-21 (2017). PMID 28697336.
user_report	Mori, Y., All-trans retinoic acid induces lipophagy through the activation of the AMPK-Beclin1 signaling pathway and reduces Rubicon expression in adipocytes. J. Nutr. Biochem. 126: 109589 (2024). PMID 38295886. [link to RRC of NBRP]
user_report	Nguyen, A.T., All-trans retinoic acid induces lipophagy by reducing Rubicon in Hepa1c1c7 cells. J. Lipid Res. 65 (8): 100598 (2024). PMID 39032560. [link to RRC of NBRP]
user_report	Hirose, S., NRF3 activates mTORC1 arginine-dependently for cancer cell viability. iScience 26 (2): 106045 (2023). PMID 36818298. [link to RRC of NBRP]
user_report	Ohnishi, K., Identification of Dietary Phytochemicals Capable of Enhancing the Autophagy Flux in HeLa and Caco-2 Human Cell Lines. Antioxidants (Basel) 9 (12): 1193 (2020). PMID 33261065. [link to RRC of NBRP]

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