Resource data sheet
DNA Bank Top
Please review the HTS results indicated by check icon below as well as clone information before placing your order.

p3xFLAG-CMV10-hAtg13

Transient expression vector of human Atg13 with N-terminal 3×FLAG.

Catalog number RDB19771
Resource name p3xFLAG-CMV10-hAtg13
Clone info. Transient expression vector of human Atg13 with N-terminal 3×FLAG.
Vector backbone p3xFLAG-CMV-10 (plasmid)
Size of vector backbone 6.3 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Gene/insert name human ATG13 cDNA; synthetic 3×FLAG-tag.
Depositor|Developer Mizushima, Noboru |
 
Sequence (full) RDB19771hts01.seqcheck assembled from experimentally sequenced data
Test sheet RDB19771_B2Jlp1-1.pdf 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Hosokawa, N. et al. Mol. Biol. Cell. 20(7): 1981-1991, 2009)
Ordering
Order form [Credit Card Payment]  [Bank Transfer Payment]
MTA, for use for not-for-profit academic purpose [Word]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
提供案内 (日本国内) [open/close]

提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Hosokawa, N. et al. Mol. Biol. Cell. 20(7): 1981-1991, 2009)。
提供依頼
提供依頼書 [Word]
提供同意書 (MTA, 非営利学術目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19771 p3xFLAG-CMV10-hAtg13 DNA solution


How to cite this biological resource

Materials & Methods section:

The p3xFLAG-CMV10-hAtg13 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19771).

Reference section:

Hosokawa, N., Hara, T., Kaizuka, T., Kishi, C., Takamura, A., Miura, Y., Iemura, S., Natsume, T., Takehana, K., Yamada, N., Guan, J.L., Oshiro, N., Mizushima, N., Nutrient-dependent mTORC1 association with the ULK1-Atg13-FIP200 complex required for autophagy. Mol. Biol. Cell 20 (7): 1981-1991 (2009). PMID 19211835. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles

original Hosokawa, N., Nutrient-dependent mTORC1 association with the ULK1-Atg13-FIP200 complex required for autophagy. Mol. Biol. Cell 20 (7): 1981-1991 (2009). PMID 19211835. [link to RRC of NBRP]