RDB19962 - pMRX-IB-pSu9-HaloTag7-mGFP

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pMRX-IB-pSu9-HaloTag7-mGFP

Retroviral vector for stable expression of pSu9-HaloTag7-mGFP to measure mitophagy activity.

Catalog number	RDB19962
Resource name	pMRX-IB-pSu9-HaloTag7-mGFP
Clone info.	Retroviral vector for stable expression of pSu9-HaloTag7-mGFP harboring a blasticidin-resistant marker. pSu9, subunit 9 of the mitochondrial F1F0-ATPase (Su9) from Neurospora crassa (residues 1–69) to measure mitophagy activity. See, Yim, W.WY. et al., eLife 11: e78923, 2022.
Comment	Expression was confirmed by the depositor with western blotting.
Vector backbone	pMRX-IB (plasmid)
Selectable markers	Ampicillin (E. coli), bsr (mammalian cell)
Gene/insert name	Neurospora crassa mitochondrial F1F0-ATPase (Su9); Rhodococcus rhodochrous HaloTag7; Aequorea victoria GFP (mGFP).
Depositor\|Developer	Mizushima, Noboru \|

Other clones in our bank

Neurospora crassa Fo-ATPase subunit 9 and ortholog

External Database
Neurospora crassa Fo-ATPase subunit 9

Reference sequence

Sequence (full)	RDB19962hts01.seq assembled from experimentally sequenced data
Test sheet	RDB19962_B3Dsp1-1.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(Willa Wen-You Yim et al. eLife. 11:e78923, 2022)
Additional terms and conditions:
Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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提供案内 (日本国内) [open/close]

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(Willa Wen-You Yim et al. eLife. 11:e78923, 2022) Additional terms and conditions: Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested. The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
Ordering	Order form [Credit Card Payment] [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] Please visit Information of Request for Distribution.[link]
Remarks	Remember that you will be working with samples containing infectious virus.

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用すること(Willa Wen-You Yim et al. eLife. 11:e78923, 2022)。付加的提供条件： pMRXベクターについて：利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献（Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB19962	pMRX-IB-pSu9-HaloTag7-mGFP	DNA solution

How to cite this biological resource

Materials & Methods section:

The pMRX-IB-pSu9-HaloTag7-mGFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19962).

Reference section:

Yim, W.W., Yamamoto, H., Mizushima, N., A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag. Elife 11: e78923 (2022). PMID 35938926. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

Tips

Featured content	Autophagy (English text)

References

Original, user report and related articles

original	Yim, W.W., A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag. Elife 11: e78923 (2022). PMID 35938926. [link to RRC of NBRP]

2024.03.29

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