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pMRX-IB-pSu9-HaloTag7-mGFP (#RDB19962)

Retroviral vector for stable expression of pSu9-HaloTag7-mGFP to measure mitophagy activity.


Drawn by SnapGene® software
Sequence (full) RDB19962hts01.seq
Publication Yim, W.W., Elife 11: e78923 (2022). [link to RRC of NBRP]
Test sheet RDB19962_B3Dsp1-1.pdf 
Assembled from experimentally sequenced data.
 
Clone info. Retroviral vector for stable expression of pSu9-HaloTag7-mGFP harboring a blasticidin-resistant marker. pSu9, subunit 9 of the mitochondrial F1F0-ATPase (Su9) from Neurospora crassa (residues 1–69) to measure mitophagy activity. See, Yim, W.WY. et al., eLife 11: e78923, 2022.
Comment Expression was confirmed by the depositor with western blotting.
Vector backbone pMRX-IB (plasmid)
Selectable markers Ampicillin (E. coli), bsr (mammalian cell)
Growth conditions LB+Amp, 37oC
Gene/insert name Neurospora crassa mitochondrial F1F0-ATPase (Su9); Rhodococcus rhodochrous HaloTag7; Aequorea victoria GFP (mGFP).
Depositor|Developer Mizushima, Noboru |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(Willa Wen-You Yim et al. eLife. 11:e78923, 2022)
Additional terms and conditions:
Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用すること(Willa Wen-You Yim et al. eLife. 11:e78923, 2022)。
MTAに書く付加的使用条件：
pMRXベクターについて：利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19962 pMRX-IB-pSu9-HaloTag7-mGFP DNA solution

How to cite this biological resource

Materials & Methods section:

The pMRX-IB-pSu9-HaloTag7-mGFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19962).

Reference section:

Yim, W.W., Yamamoto, H., Mizushima, N., A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag. Elife 11: e78923 (2022). PMID 35938926. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Yim, W.W., A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag. Elife 11: e78923 (2022). PMID 35938926. [link to RRC of NBRP]