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pMRX-IP-SECFP-hATG16L1 (#RDB19635)

Retroviral vector for stable expression of human ATG16L1 with N-terminal SECFP.

Clone info. Retroviral vector for stable expression of human ATG16L1 with N-terminal SECFP.
Vector backbone pMRX-IP (plasmid)
Selectable markers Ampicillin (E. coli), Puromycin (mammalian cell)
Gene/insert name human ATG16L1; Aequorea victoria GFP (SECFP).
Depositor|Developer Mizushima, Noboru |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Approval form [Word] by Dr. Atsushi Miyawaki
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Koyama-Honda, I. et al. Autophagy, 9(10), 1491-1499, 2013)
Additional terms and conditions:
Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
Remarks Remember that you will be working with samples containing infectious virus.
Please obtain written approval of Dr. Atsushi Miyawaki.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
宮脇敦史博士による提供承諾書 [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Koyama-Honda, I. et al. Autophagy, 9(10), 1491-1499, 2013)。
MTAに書く付加的使用条件：
pMRXベクターについて：利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
宮脇敦史博士の書面による提供承諾が必要です。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB19635 pMRX-IP-SECFP-hATG16L1 Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pMRX-IP-SECFP-hATG16L1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19635).

Reference section:

Koyama-Honda, I., Itakura, E., Fujiwara, T.K., Mizushima, N., Temporal analysis of recruitment of mammalian ATG proteins to the autophagosome formation site. Autophagy 9 (10): 1491-1499 (2013). PMID 23884233. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Koyama-Honda, I., Temporal analysis of recruitment of mammalian ATG proteins to the autophagosome formation site. Autophagy 9 (10): 1491-1499 (2013). PMID 23884233. [link to RRC of NBRP]