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pEGFP-C1-mApg5(K130R) (#RDB19789)

Transient expression vector of mouse Apg5 mutant (K130R) with N-terminal GFP.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Mizushima, N., J. Cell Biol. 152 (4): 657-668 (2001). PMID 11266458. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Transient expression vector of mouse Apg5 mutant (K130R) with N-terminal GFP.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cell)
Growth conditions LB+Kanamycin, 37oC
Gene/insert name mouse Atg5 cDNA; Aequorea victoria GFP (EGFP).
Depositor|Developer Mizushima, Noboru |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Mizushima, N. et al. J. Cell Biol. 152(4): 657-668, 2001)
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必要書類
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提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Mizushima, N. et al. J. Cell Biol. 152(4): 657-668, 2001)。

Catalog # Resource name Shipping form Fee
RDB19789 pEGFP-C1-mApg5(K130R) DNA solution

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-mApg5(K130R) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19789).

Reference section:

Mizushima, N., Yamamoto, A., Hatano, M., Kobayashi, Y., Kabeya, Y., Suzuki, K., Tokuhisa, T., Ohsumi, Y., Yoshimori, T., Dissection of autophagosome formation using Apg5-deficient mouse embryonic stem cells. J. Cell Biol. 152 (4): 657-668 (2001). PMID 11266458. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Mizushima, N., Dissection of autophagosome formation using Apg5-deficient mouse embryonic stem cells. J. Cell Biol. 152 (4): 657-668 (2001). PMID 11266458. [PubMed] [Article] [RRC of NBRP]