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pMXs-IP-EGFP-LC3 (#RDB20244)

Retroviral vector for stable expression of rat LC3 with N-terminal EGFP.


Drawn by SnapGene® software
Sequence (full) RDB20244hts01.seq
Publication Hara, T., J. Cell Biol. 181 (3): 497-510 (2008). [link to RRC of NBRP]
Test sheet RDB20244_B4App1-1.pdf 
Assembled from experimentally sequenced data.
 
Clone info. Retroviral vector for stable expression of rat LC3 with N-terminal EGFP.
Vector backbone pMRXIP GFP (plasmid)
Selectable markers Ampicillin (E. coli), Puromycin (mammalian cell)
Gene/insert name rat Map1lc3b cDNA; Aequorea victoria GFP (EGFP).
Depositor|Developer Mizushima, Noboru |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Hara, T. et al. J. Cell Biol. 181(3): 497-510, 2008)
Additional terms and conditions:
Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Hara, T. et al. J. Cell Biol. 181(3): 497-510, 2008)。
MTAに書く付加的使用条件：
pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB20244 pMXs-IP-EGFP-LC3 DNA solution

How to cite this biological resource

Materials & Methods section:

The pMXs-IP-EGFP-LC3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20244).

Reference section:

Hara, T., Takamura, A., Kishi, C., Iemura, S., Natsume, T., Guan, J.L., Mizushima, N., FIP200, a ULK-interacting protein, is required for autophagosome formation in mammalian cells. J. Cell Biol. 181 (3): 497-510 (2008). PMID 18443221. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Hara, T., FIP200, a ULK-interacting protein, is required for autophagosome formation in mammalian cells. J. Cell Biol. 181 (3): 497-510 (2008). PMID 18443221. [link to RRC of NBRP]