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Catalog # | Name of clone | Running title | Publication |
RDB19976 | pCAG-1700029I15Rik-3×FLAG | Expression vector of mouse 1700029I15Rik. 3XFLAG tag at C-terminus. | Lu, Y., 1700029I15Rik orchestrates the biosynthesis of acrosomal membrane proteins required for sperm-egg interaction. Proc. Natl. Acad. Sci. U.S.A. 120 (8): e2207263120 (2023). PMID 36787362. (original). [link to RRC of NBRP] |
RDB19975 | pCAG1.1-HA/Ppp1cc2 | Expression vector of mouse PPP1CC2. HA tag at N-terminus. | Shimada, K., TSKS localizes to nuage in spermatids and regulates cytoplasmic elimination during spermiation. Proc. Natl. Acad. Sci. U.S.A. 120 (11): e2221762120 (2023). PMID 36881620. (original). [link to RRC of NBRP] |
RDB19974 | pCAG1.1-TSSK2/3xFLAG | Expression vector of mouse TSSK2. 3xFLAG tag at C-terminus. | Shimada, K., TSKS localizes to nuage in spermatids and regulates cytoplasmic elimination during spermiation. Proc. Natl. Acad. Sci. U.S.A. 120 (11): e2221762120 (2023). PMID 36881620. (original). [link to RRC of NBRP] |
RDB19973 | pCAG1.1-Myc/TSSK1 | Expression vector of mouse TSSK1. Myc-tag at N-terminus. | Shimada, K., TSKS localizes to nuage in spermatids and regulates cytoplasmic elimination during spermiation. Proc. Natl. Acad. Sci. U.S.A. 120 (11): e2221762120 (2023). PMID 36881620. (original). [link to RRC of NBRP] |
RDB19972 | pCAG1.1-Tsks/1D4 | Expression vector of mouse TSKS (variant X6). 1D4 tag at C-terminus. | Shimada, K., TSKS localizes to nuage in spermatids and regulates cytoplasmic elimination during spermiation. Proc. Natl. Acad. Sci. U.S.A. 120 (11): e2221762120 (2023). PMID 36881620. (original). [link to RRC of NBRP] |
RDB19674 | pClgn1.1_Dcst2-3xHA | Expression vector of mouse Dcst2-3xHA under the mouse Clgn promoter. | Noda, T., Sperm membrane proteins DCST1 and DCST2 are required for sperm-egg interaction in mice and fish. Commun. Biol. 5 (1): 332 (2022). PMID 35393517. (original). [link to RRC of NBRP] |
RDB19673 | pClgn1.1_Dcst1-3xHA | Expression vector of mouse Dcst1-3xHA under the mouse Clgn promoter. | Noda, T., Sperm membrane proteins DCST1 and DCST2 are required for sperm-egg interaction in mice and fish. Commun. Biol. 5 (1): 332 (2022). PMID 35393517. (original). [link to RRC of NBRP] |
RDB19672 | pCAG-Izumo1-1D4 | Expression vector of mouse Izumo1-1D4 under the CAG promoter. | Noda, T., Sperm membrane proteins DCST1 and DCST2 are required for sperm-egg interaction in mice and fish. Commun. Biol. 5 (1): 332 (2022). PMID 35393517. (original). [link to RRC of NBRP] |
RDB19671 | pCAG-Dcst2-3xHA | Expression vector of mouse Dcst2-3xHA under the CAG promoter. | Noda, T., Sperm membrane proteins DCST1 and DCST2 are required for sperm-egg interaction in mice and fish. Commun. Biol. 5 (1): 332 (2022). PMID 35393517. (original). [link to RRC of NBRP] |
RDB19670 | pCAG-Dcst1-3xFLAG | Expression vector of mouse Dcst1-3xFLAG under the CAG promoter. | Noda, T., Sperm membrane proteins DCST1 and DCST2 are required for sperm-egg interaction in mice and fish. Commun. Biol. 5 (1): 332 (2022). PMID 35393517. (original). [link to RRC of NBRP] |
RDB19663 | pClgn-Tesmin-TurboID-3xFLAG | Expression vector of mouse Tesmin tagged with TurboID-3xFLAG at C-terminus. mouse Clgn promoter. | Oura, S., Proximity-dependent biotin labeling in testicular germ cells identified TESMIN-associated proteins. Sci. Rep. 12 (1): 22198 (2022). PMID 36564444. (original). [link to RRC of NBRP] |
RDB19662 | pClgn-Tesmin-BioID2-3xFLAG | Expression vector of mouse Tesmin tagged with BioID2-3xFLAG at C-terminus. mouse Clgn promoter. | Oura, S., Proximity-dependent biotin labeling in testicular germ cells identified TESMIN-associated proteins. Sci. Rep. 12 (1): 22198 (2022). PMID 36564444. (original). [link to RRC of NBRP] |
RDB19661 | pCAG-Tesmin-BioID2-3xFLAG | Expression vector of mouse Tesmin tagged with BioID2-3xFLAG at C-terminus. CAG promoter. | Oura, S., Proximity-dependent biotin labeling in testicular germ cells identified TESMIN-associated proteins. Sci. Rep. 12 (1): 22198 (2022). PMID 36564444. (original). [link to RRC of NBRP] |
RDB19660 | pCAG-MCS-TurboID-3xFLAG | CAG promoter-driven expression of TurboID-3xFLAG-fused protein (C terminus). | Oura, S., Proximity-dependent biotin labeling in testicular germ cells identified TESMIN-associated proteins. Sci. Rep. 12 (1): 22198 (2022). PMID 36564444. (original). [link to RRC of NBRP] |
RDB19659 | pCAG-MCS-BioID2-3xFLAG | CAG promoter-driven expression of BioID2-3xFLAG-fused protein (C terminus). | Oura, S., Proximity-dependent biotin labeling in testicular germ cells identified TESMIN-associated proteins. Sci. Rep. 12 (1): 22198 (2022). PMID 36564444. (original). [link to RRC of NBRP] |
RDB19658 | pCAG-3xFLAG-TurboID-MCS | CAG promoter-driven expression of 3xFLAG-TurboID-fused protein (N terminus). | Oura, S., Proximity-dependent biotin labeling in testicular germ cells identified TESMIN-associated proteins. Sci. Rep. 12 (1): 22198 (2022). PMID 36564444. (original). [link to RRC of NBRP] |
RDB19657 | pCAG-3xFLAG-BioID2-MCS | CAG promoter-driven expression of 3xFLAG-BioID2-fused protein (N terminus). | Oura, S., Proximity-dependent biotin labeling in testicular germ cells identified TESMIN-associated proteins. Sci. Rep. 12 (1): 22198 (2022). PMID 36564444. (original). [link to RRC of NBRP] |
RDB19656 | pClgn1.1-Trim41-PA-1D4 | Expression vector of mouse Trim41under the control of Clgn promoter. | Oura, S., Trim41 is required to regulate chromosome axis protein dynamics and meiosis in male mice. PLoS Genet. 18 (6): e1010241 (2022). PMID 35648791. (original). [link to RRC of NBRP] |
RDB19467 | pCMV-Armc12/FLAG/BioID2/HA | Expression vector of mouse Armc12 fused with FLAG, BirA and HA (Armc12-FLAG-BioID2-HA). | Shimada, K., ARMC12 regulates spatiotemporal mitochondrial dynamics during spermiogenesis and is required for male fertility. Proc Natl Acad Sci U S A. 2021 Feb 9;118(6):e2018355118 (2021). PMID 33536340. (original). [link to RRC of NBRP] |
RDB19466 | pCAG1.1-Armc12 delta C/FLAG | Expression vector of C-terminal deleted mouse Armc12 tagged with FLAG at C-terminus. | Shimada, K., ARMC12 regulates spatiotemporal mitochondrial dynamics during spermiogenesis and is required for male fertility. Proc Natl Acad Sci U S A. 2021 Feb 9;118(6):e2018355118 (2021). PMID 33536340. (original). [link to RRC of NBRP] |
RDB19465 | pCAG1.1-Armc12 delta ARM/FLAG | Expression vector of ARM domain deleted mouse Armc12 tagged with FLAG at C-terminus. | Shimada, K., ARMC12 regulates spatiotemporal mitochondrial dynamics during spermiogenesis and is required for male fertility. Proc Natl Acad Sci U S A. 2021 Feb 9;118(6):e2018355118 (2021). PMID 33536340. (original). [link to RRC of NBRP] |
RDB19464 | pCAG1.1-Armc12 delta N/FLAG | Expression vector of N-terminal deleted mouse Armc12 tagged with FLAG at C-terminus. | Shimada, K., ARMC12 regulates spatiotemporal mitochondrial dynamics during spermiogenesis and is required for male fertility. Proc Natl Acad Sci U S A. 2021 Feb 9;118(6):e2018355118 (2021). PMID 33536340. (original). [link to RRC of NBRP] |
RDB19463 | pCAG1.1-Armc12/FLAG | Expression vector of mouse Armc12 tagged with FLAG at C-terminus. | Shimada, K., ARMC12 regulates spatiotemporal mitochondrial dynamics during spermiogenesis and is required for male fertility. Proc Natl Acad Sci U S A. 2021 Feb 9;118(6):e2018355118 (2021). PMID 33536340. (original). [link to RRC of NBRP] |
RDB19440 | pClgn-Lrrc23-1D4 | Expression vector of mouse Lrrc23 tagged with 1D4 at C-terminus, driven by Calmergin promoter. | Zhang, X., LRRC23 is a conserved component of the radial spoke that is necessary for sperm motility and male fertility in mice. J. Cell Sci. 134 (20): jcs259381 (2021). PMID 34585727. (original). [link to RRC of NBRP] |
RDB19439 | pClgn-Lrrc23-FLAG | Expression vector of mouse Lrrc23 tagged with FLAG at C-terminus, driven by Calmergin promoter. | Zhang, X., LRRC23 is a conserved component of the radial spoke that is necessary for sperm motility and male fertility in mice. J. Cell Sci. 134 (20): jcs259381 (2021). PMID 34585727. (original). [link to RRC of NBRP] |
RDB19409 | pCAG-hPPP3R2 | Expression vector of human PPP3R2. | Miyata, H., Sperm calcineurin inhibition prevents mouse fertility with implications for male contraceptive. Science 350 (6259): 442-445 (2015). PMID 26429887. (original). [link to RRC of NBRP] |
RDB19408 | pCAG-hPPP3CC-FLAG | Expression vector of human PPP3CC with FLAG-tag. | Miyata, H., Sperm calcineurin inhibition prevents mouse fertility with implications for male contraceptive. Science 350 (6259): 442-445 (2015). PMID 26429887. (original). [link to RRC of NBRP] |
RDB19407 | pCAG-mPpp3r2 | Expression vector of mouse Ppp3r2. | Miyata, H., Sperm calcineurin inhibition prevents mouse fertility with implications for male contraceptive. Science 350 (6259): 442-445 (2015). PMID 26429887. (original). [link to RRC of NBRP] |
RDB19406 | pCAG-mPpp3cc-FLAG-6XHIS | Expression vector of mouse Ppp3cc with FLAG- and 6xHIS-tags. | Miyata, H., Sperm calcineurin inhibition prevents mouse fertility with implications for male contraceptive. Science 350 (6259): 442-445 (2015). PMID 26429887. (original). [link to RRC of NBRP] |
RDB19405 | pCAG-hPPP3R1 | Expression vector of human PPP3R1. | Miyata, H., SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice. Proc. Natl. Acad. Sci. U.S.A. 118 (35): e2106673118 (2021). PMID 34446558. (original). [link to RRC of NBRP] |
RDB19404 | pCAG-hPPP3CB-FLAG | Expression vector of human PPP3CB with FLAG-tag. | Miyata, H., SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice. Proc. Natl. Acad. Sci. U.S.A. 118 (35): e2106673118 (2021). PMID 34446558. (original). [link to RRC of NBRP] |
RDB19403 | pCAG-hPPP3CA-FLAG | Expression vector of human PPP3CA with FLAG-tag. | Miyata, H., SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice. Proc. Natl. Acad. Sci. U.S.A. 118 (35): e2106673118 (2021). PMID 34446558. (original). [link to RRC of NBRP] |
RDB19402 | pCAG-hSPATA33-8xHIS-1D4 | Expression vector of human SPATA33 with 8xHIS- and 1D4-tags. | Miyata, H., SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice. Proc. Natl. Acad. Sci. U.S.A. 118 (35): e2106673118 (2021). PMID 34446558. (original). [link to RRC of NBRP] |
RDB19401 | pCAG-mSpata33 (I83A)-PA | Expression vector of mouse Spata33 with PA-tag. I83A mutation is introduced in SPATA33 (PQIIAT). | Miyata, H., SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice. Proc. Natl. Acad. Sci. U.S.A. 118 (35): e2106673118 (2021). PMID 34446558. (original). [link to RRC of NBRP] |
RDB19400 | pCAG-mSpata33 (I81A)-PA | Expression vector of mouse Spata33 with PA-tag. I81A mutation is introduced in SPATA33 (PQAIIT). | Miyata, H., SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice. Proc. Natl. Acad. Sci. U.S.A. 118 (35): e2106673118 (2021). PMID 34446558. (original). [link to RRC of NBRP] |
RDB19399 | pCAG-mSpata33 (P79A)-PA | Expression vector of mouse Spata33 with PA-tag. P79A mutation is introduced in SPATA33 (AQIIIT). | Miyata, H., SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice. Proc. Natl. Acad. Sci. U.S.A. 118 (35): e2106673118 (2021). PMID 34446558. (original). [link to RRC of NBRP] |
RDB19398 | pCAG-mSpata33-PA | Expression vector of mouse Spata33 with PA-tag. | Miyata, H., SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice. Proc. Natl. Acad. Sci. U.S.A. 118 (35): e2106673118 (2021). PMID 34446558. (original). [link to RRC of NBRP] |
RDB19249 | pClgn1.1_3xFLAG-Kctd19 | Expression vector of mouse Kctd19 tagged with 3xFLAG at N-terminus. | Oura, S., KCTD19 and its associated protein ZFP541 are independently essential for meiosis in male mice. PLoS Genet. 17 (5): e1009412 (2021). PMID 33961623. (original). [link to RRC of NBRP] |
RDB19248 | pClgn1.1_Kctd19-3xHA | Expression vector of mouse Kctd19 tagged with 3xHA at C-terminus. | Oura, S., KCTD19 and its associated protein ZFP541 are independently essential for meiosis in male mice. PLoS Genet. 17 (5): e1009412 (2021). PMID 33961623. (original). [link to RRC of NBRP] |
RDB19247 | pClgn1.1 | Mammalian expression vector based on calmegin promoter. | Ikawa, M., Calmegin is required for fertilin alpha/beta heterodimerization and sperm fertility. Dev. Biol. 240 (1): 254-261 (2001). PMID 11784061. (original). [link to RRC of NBRP] |
RDB19246 | pCAG1.1 | Mammalian expression vector based on CAG promoter. A derivative of the pCAGGS vector. | Ikawa, M., Calsperin is a testis-specific chaperone required for sperm fertility. J. Biol. Chem. 286 (7): 5639-5646 (2011). PMID 21131354. (original). [link to RRC of NBRP] |
RDB19243 | pCAG-mDrc7-FLAG | Expression vector of mouse Drc7 tagged with FLAG. | Morohoshi, A., Nexin-Dynein regulatory complex component DRC7 but not FBXL13 is required for sperm flagellum formation and male fertility in mice. PLoS Genet. 16 (1): e1008585 (2020). PMID 31961863. (original). [link to RRC of NBRP] |
RDB19242 | pCAG-mTcte1-PA | Expression vector of mouse Tcte1 tagged with PA. | Morohoshi, A., Nexin-Dynein regulatory complex component DRC7 but not FBXL13 is required for sperm flagellum formation and male fertility in mice. PLoS Genet. 16 (1): e1008585 (2020). PMID 31961863. (original). [link to RRC of NBRP] |
RDB19241 | pCAG-mGas8-PA | Expression vector of mouse Gas8 tagged with PA. | Morohoshi, A., Nexin-Dynein regulatory complex component DRC7 but not FBXL13 is required for sperm flagellum formation and male fertility in mice. PLoS Genet. 16 (1): e1008585 (2020). PMID 31961863. (original). [link to RRC of NBRP] |
RDB19240 | pCAG-mDrc3-PA | Expression vector of mouse Drc3 tagged with PA. | Morohoshi, A., Nexin-Dynein regulatory complex component DRC7 but not FBXL13 is required for sperm flagellum formation and male fertility in mice. PLoS Genet. 16 (1): e1008585 (2020). PMID 31961863. (original). [link to RRC of NBRP] |
RDB17453 | pCAG_mRsph6a-FLAG | Expression vector of mouse Rsph6a. | Abbasi, F., RSPH6A is required for sperm flagellum formation and male fertility in mice. J. Cell Sci. 131 (19). pii: jcs221648 (2018). PMID 30185526. (original). [link to RRC of NBRP] |
RDB17452 | pCAG_mRsph10b-PA | Expression vector of mouse Rsph10b. | Abbasi, F., RSPH6A is required for sperm flagellum formation and male fertility in mice. J. Cell Sci. 131 (19). pii: jcs221648 (2018). PMID 30185526. (original). [link to RRC of NBRP] |
RDB17451 | pCAG_mRsph9-PA | Expression vector of mouse Rsph9. | Abbasi, F., RSPH6A is required for sperm flagellum formation and male fertility in mice. J. Cell Sci. 131 (19). pii: jcs221648 (2018). PMID 30185526. (original). [link to RRC of NBRP] |
RDB17450 | pCAG_mRsph4a-PA | Expression vector of mouse Rsph4a. | Abbasi, F., RSPH6A is required for sperm flagellum formation and male fertility in mice. J. Cell Sci. 131 (19). pii: jcs221648 (2018). PMID 30185526. (original). [link to RRC of NBRP] |
RDB17449 | pCAG_mRsph1-PA | Expression vector of mouse Rsph1. | Abbasi, F., RSPH6A is required for sperm flagellum formation and male fertility in mice. J. Cell Sci. 131 (19). pii: jcs221648 (2018). PMID 30185526. (original). [link to RRC of NBRP] |
RDB13534 | pX330-Prm1/sgRNA#1 | Expression plasmid targeting mouse Prm1 gene in CRISPR/Cas9 system. | Mashiko, D., Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA. Sci. Rep. 3: 3355 (2013). PMID 24284873. (original). [link to RRC of NBRP] |
RDB13533 | pX330-Cetn1/sgRNA#1 | Expression plasmid targeting mouse Cetn1 gene in CRISPR/Cas9 system. | Mashiko, D., Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA. Sci. Rep. 3: 3355 (2013). PMID 24284873. (original). [link to RRC of NBRP] |
RDB13532 | pCAG-EGxxFP | Validation plasmid for the effectiveness of guide RNA in CRISPR/Cas9 system. | Mashiko, D., Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA. Sci. Rep. 3: 3355 (2013). PMID 24284873. (original). [link to RRC of NBRP] |
2024.10.04
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