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Masahito Ikawa's Resource

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Catalog #Name of cloneRunning titlePublication
RDB13532pCAG-EGxxFPValidation plasmid for the effectiveness of guide RNA in CRISPR/Cas9 system.Mashiko, D., Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA. Sci. Rep. 3: 3355 (2013). PMID 24284873. (original). [link to RRC of NBRP]
RDB13533pX330-Cetn1/sgRNA#1Expression plasmid targeting mouse Cetn1 gene in CRISPR/Cas9 system.Mashiko, D., Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA. Sci. Rep. 3: 3355 (2013). PMID 24284873. (original). [link to RRC of NBRP]
RDB13534pX330-Prm1/sgRNA#1Expression plasmid targeting mouse Prm1 gene in CRISPR/Cas9 system.Mashiko, D., Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA. Sci. Rep. 3: 3355 (2013). PMID 24284873. (original). [link to RRC of NBRP]
RDB17449pCAG_mRsph1-PAExpression vector of mouse Rsph1.Abbasi, F., RSPH6A is required for sperm flagellum formation and male fertility in mice. J. Cell Sci. 131 (19). pii: jcs221648 (2018). PMID 30185526. (original). [link to RRC of NBRP]
RDB17450pCAG_mRsph4a-PAExpression vector of mouse Rsph4a.Abbasi, F., RSPH6A is required for sperm flagellum formation and male fertility in mice. J. Cell Sci. 131 (19). pii: jcs221648 (2018). PMID 30185526. (original). [link to RRC of NBRP]
RDB17451pCAG_mRsph9-PAExpression vector of mouse Rsph9.Abbasi, F., RSPH6A is required for sperm flagellum formation and male fertility in mice. J. Cell Sci. 131 (19). pii: jcs221648 (2018). PMID 30185526. (original). [link to RRC of NBRP]
RDB17452pCAG_mRsph10b-PAExpression vector of mouse Rsph10b.Abbasi, F., RSPH6A is required for sperm flagellum formation and male fertility in mice. J. Cell Sci. 131 (19). pii: jcs221648 (2018). PMID 30185526. (original). [link to RRC of NBRP]
RDB17453pCAG_mRsph6a-FLAGExpression vector of mouse Rsph6a.Abbasi, F., RSPH6A is required for sperm flagellum formation and male fertility in mice. J. Cell Sci. 131 (19). pii: jcs221648 (2018). PMID 30185526. (original). [link to RRC of NBRP]
RDB19240pCAG-mDrc3-PAExpression vector of mouse Drc3 tagged with PA.Morohoshi, A., Nexin-Dynein regulatory complex component DRC7 but not FBXL13 is required for sperm flagellum formation and male fertility in mice. PLoS Genet. 16 (1): e1008585 (2020). PMID 31961863. (original). [link to RRC of NBRP]
RDB19241pCAG-mGas8-PAExpression vector of mouse Gas8 tagged with PA.Morohoshi, A., Nexin-Dynein regulatory complex component DRC7 but not FBXL13 is required for sperm flagellum formation and male fertility in mice. PLoS Genet. 16 (1): e1008585 (2020). PMID 31961863. (original). [link to RRC of NBRP]
RDB19242pCAG-mTcte1-PAExpression vector of mouse Tcte1 tagged with PA.Morohoshi, A., Nexin-Dynein regulatory complex component DRC7 but not FBXL13 is required for sperm flagellum formation and male fertility in mice. PLoS Genet. 16 (1): e1008585 (2020). PMID 31961863. (original). [link to RRC of NBRP]
RDB19243pCAG-mDrc7-FLAGExpression vector of mouse Drc7 tagged with FLAG.Morohoshi, A., Nexin-Dynein regulatory complex component DRC7 but not FBXL13 is required for sperm flagellum formation and male fertility in mice. PLoS Genet. 16 (1): e1008585 (2020). PMID 31961863. (original). [link to RRC of NBRP]
RDB19246pCAG1.1Mammalian expression vector based on CAG promoter. A derivative of the pCAGGS vector.Ikawa, M., Calsperin is a testis-specific chaperone required for sperm fertility. J. Biol. Chem. 286 (7): 5639-5646 (2011). PMID 21131354. (original). [link to RRC of NBRP]
RDB19247pClgn1.1Mammalian expression vector based on calmegin promoter.Ikawa, M., Calmegin is required for fertilin alpha/beta heterodimerization and sperm fertility. Dev. Biol. 240 (1): 254-261 (2001). PMID 11784061. (original). [link to RRC of NBRP]
RDB19248pClgn1.1_Kctd19-3xHAExpression vector of mouse Kctd19 tagged with 3xHA at C-terminus.Oura, S., KCTD19 and its associated protein ZFP541 are independently essential for meiosis in male mice. PLoS Genet. 17 (5): e1009412 (2021). PMID 33961623. (original). [link to RRC of NBRP]
RDB19249pClgn1.1_3xFLAG-Kctd19Expression vector of mouse Kctd19 tagged with 3xFLAG at N-terminus.Oura, S., KCTD19 and its associated protein ZFP541 are independently essential for meiosis in male mice. PLoS Genet. 17 (5): e1009412 (2021). PMID 33961623. (original). [link to RRC of NBRP]
RDB19398pCAG-mSpata33-PAExpression vector of mouse Spata33 with PA-tag.Miyata, H., SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice. Proc. Natl. Acad. Sci. U.S.A. 118 (35): e2106673118 (2021). PMID 34446558. (original). [link to RRC of NBRP]
RDB19399pCAG-mSpata33 (P79A)-PAExpression vector of mouse Spata33 with PA-tag. P79A mutation is introduced in SPATA33 (AQIIIT).Miyata, H., SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice. Proc. Natl. Acad. Sci. U.S.A. 118 (35): e2106673118 (2021). PMID 34446558. (original). [link to RRC of NBRP]
RDB19400pCAG-mSpata33 (I81A)-PAExpression vector of mouse Spata33 with PA-tag. I81A mutation is introduced in SPATA33 (PQAIIT).Miyata, H., SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice. Proc. Natl. Acad. Sci. U.S.A. 118 (35): e2106673118 (2021). PMID 34446558. (original). [link to RRC of NBRP]
RDB19401pCAG-mSpata33 (I83A)-PAExpression vector of mouse Spata33 with PA-tag. I83A mutation is introduced in SPATA33 (PQIIAT).Miyata, H., SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice. Proc. Natl. Acad. Sci. U.S.A. 118 (35): e2106673118 (2021). PMID 34446558. (original). [link to RRC of NBRP]
RDB19402pCAG-hSPATA33-8xHIS-1D4Expression vector of human SPATA33 with 8xHIS- and 1D4-tags.Miyata, H., SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice. Proc. Natl. Acad. Sci. U.S.A. 118 (35): e2106673118 (2021). PMID 34446558. (original). [link to RRC of NBRP]
RDB19403pCAG-hPPP3CA-FLAGExpression vector of human PPP3CA with FLAG-tag.Miyata, H., SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice. Proc. Natl. Acad. Sci. U.S.A. 118 (35): e2106673118 (2021). PMID 34446558. (original). [link to RRC of NBRP]
RDB19404pCAG-hPPP3CB-FLAGExpression vector of human PPP3CB with FLAG-tag.Miyata, H., SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice. Proc. Natl. Acad. Sci. U.S.A. 118 (35): e2106673118 (2021). PMID 34446558. (original). [link to RRC of NBRP]
RDB19405pCAG-hPPP3R1Expression vector of human PPP3R1.Miyata, H., SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice. Proc. Natl. Acad. Sci. U.S.A. 118 (35): e2106673118 (2021). PMID 34446558. (original). [link to RRC of NBRP]
RDB19406pCAG-mPpp3cc-FLAG-6XHISExpression vector of mouse Ppp3cc with FLAG- and 6xHIS-tags.Miyata, H., Sperm calcineurin inhibition prevents mouse fertility with implications for male contraceptive. Science 350 (6259): 442-445 (2015). PMID 26429887. (original). [link to RRC of NBRP]
RDB19407pCAG-mPpp3r2Expression vector of mouse Ppp3r2.Miyata, H., Sperm calcineurin inhibition prevents mouse fertility with implications for male contraceptive. Science 350 (6259): 442-445 (2015). PMID 26429887. (original). [link to RRC of NBRP]
RDB19408pCAG-hPPP3CC-FLAGExpression vector of human PPP3CC with FLAG-tag.Miyata, H., Sperm calcineurin inhibition prevents mouse fertility with implications for male contraceptive. Science 350 (6259): 442-445 (2015). PMID 26429887. (original). [link to RRC of NBRP]
RDB19409pCAG-hPPP3R2Expression vector of human PPP3R2.Miyata, H., Sperm calcineurin inhibition prevents mouse fertility with implications for male contraceptive. Science 350 (6259): 442-445 (2015). PMID 26429887. (original). [link to RRC of NBRP]
RDB19439pClgn-Lrrc23-FLAGExpression vector of mouse Lrrc23 tagged with FLAG at C-terminus, driven by Calmergin promoter.Zhang, X., LRRC23 is a conserved component of the radial spoke that is necessary for sperm motility and male fertility in mice. J. Cell Sci. 134 (20): jcs259381 (2021). PMID 34585727. (original). [link to RRC of NBRP]
RDB19440pClgn-Lrrc23-1D4Expression vector of mouse Lrrc23 tagged with 1D4 at C-terminus, driven by Calmergin promoter.Zhang, X., LRRC23 is a conserved component of the radial spoke that is necessary for sperm motility and male fertility in mice. J. Cell Sci. 134 (20): jcs259381 (2021). PMID 34585727. (original). [link to RRC of NBRP]
RDB19463pCAG1.1-Armc12/FLAGExpression vector of mouse Armc12 tagged with FLAG at C-terminus.Shimada, K., ARMC12 regulates spatiotemporal mitochondrial dynamics during spermiogenesis and is required for male fertility. Proc Natl Acad Sci U S A. 2021 Feb 9;118(6):e2018355118 (2021). PMID 33536340. (original). [link to RRC of NBRP]
RDB19464pCAG1.1-Armc12 delta N/FLAGExpression vector of N-terminal deleted mouse Armc12 tagged with FLAG at C-terminus.Shimada, K., ARMC12 regulates spatiotemporal mitochondrial dynamics during spermiogenesis and is required for male fertility. Proc Natl Acad Sci U S A. 2021 Feb 9;118(6):e2018355118 (2021). PMID 33536340. (original). [link to RRC of NBRP]
RDB19465pCAG1.1-Armc12 delta ARM/FLAGExpression vector of ARM domain deleted mouse Armc12 tagged with FLAG at C-terminus.Shimada, K., ARMC12 regulates spatiotemporal mitochondrial dynamics during spermiogenesis and is required for male fertility. Proc Natl Acad Sci U S A. 2021 Feb 9;118(6):e2018355118 (2021). PMID 33536340. (original). [link to RRC of NBRP]
RDB19466pCAG1.1-Armc12 delta C/FLAGExpression vector of C-terminal deleted mouse Armc12 tagged with FLAG at C-terminus.Shimada, K., ARMC12 regulates spatiotemporal mitochondrial dynamics during spermiogenesis and is required for male fertility. Proc Natl Acad Sci U S A. 2021 Feb 9;118(6):e2018355118 (2021). PMID 33536340. (original). [link to RRC of NBRP]
RDB19467pCMV-Armc12/FLAG/BioID2/HAExpression vector of mouse Armc12 fused with FLAG, BirA and HA (Armc12-FLAG-BioID2-HA).Shimada, K., ARMC12 regulates spatiotemporal mitochondrial dynamics during spermiogenesis and is required for male fertility. Proc Natl Acad Sci U S A. 2021 Feb 9;118(6):e2018355118 (2021). PMID 33536340. (original). [link to RRC of NBRP]
RDB19656pClgn1.1-Trim41-PA-1D4Expression vector of mouse Trim41under the control of Clgn promoter.Oura, S., Trim41 is required to regulate chromosome axis protein dynamics and meiosis in male mice. PLoS Genet. 18 (6): e1010241 (2022). PMID 35648791. (original). [link to RRC of NBRP]
RDB19657pCAG-3xFLAG-BioID2-MCSCAG promoter-driven expression of 3xFLAG-BioID2-fused protein (N terminus).Oura, S., Proximity-dependent biotin labeling in testicular germ cells identified TESMIN-associated proteins. Sci. Rep. 12 (1): 22198 (2022). PMID 36564444. (original). [link to RRC of NBRP]
RDB19658pCAG-3xFLAG-TurboID-MCSCAG promoter-driven expression of 3xFLAG-TurboID-fused protein (N terminus).Oura, S., Proximity-dependent biotin labeling in testicular germ cells identified TESMIN-associated proteins. Sci. Rep. 12 (1): 22198 (2022). PMID 36564444. (original). [link to RRC of NBRP]
RDB19659pCAG-MCS-BioID2-3xFLAGCAG promoter-driven expression of BioID2-3xFLAG-fused protein (C terminus).Oura, S., Proximity-dependent biotin labeling in testicular germ cells identified TESMIN-associated proteins. Sci. Rep. 12 (1): 22198 (2022). PMID 36564444. (original). [link to RRC of NBRP]
RDB19660pCAG-MCS-TurboID-3xFLAGCAG promoter-driven expression of TurboID-3xFLAG-fused protein (C terminus).Oura, S., Proximity-dependent biotin labeling in testicular germ cells identified TESMIN-associated proteins. Sci. Rep. 12 (1): 22198 (2022). PMID 36564444. (original). [link to RRC of NBRP]
RDB19661pCAG-Tesmin-BioID2-3xFLAGExpression vector of mouse Tesmin tagged with BioID2-3xFLAG at C-terminus. CAG promoter.Oura, S., Proximity-dependent biotin labeling in testicular germ cells identified TESMIN-associated proteins. Sci. Rep. 12 (1): 22198 (2022). PMID 36564444. (original). [link to RRC of NBRP]
RDB19662pClgn-Tesmin-BioID2-3xFLAGExpression vector of mouse Tesmin tagged with BioID2-3xFLAG at C-terminus. mouse Clgn promoter.Oura, S., Proximity-dependent biotin labeling in testicular germ cells identified TESMIN-associated proteins. Sci. Rep. 12 (1): 22198 (2022). PMID 36564444. (original). [link to RRC of NBRP]
RDB19663pClgn-Tesmin-TurboID-3xFLAGExpression vector of mouse Tesmin tagged with TurboID-3xFLAG at C-terminus. mouse Clgn promoter.Oura, S., Proximity-dependent biotin labeling in testicular germ cells identified TESMIN-associated proteins. Sci. Rep. 12 (1): 22198 (2022). PMID 36564444. (original). [link to RRC of NBRP]
RDB19670pCAG-Dcst1-3xFLAGExpression vector of mouse Dcst1-3xFLAG under the CAG promoter.Noda, T., Sperm membrane proteins DCST1 and DCST2 are required for sperm-egg interaction in mice and fish. Commun. Biol. 5 (1): 332 (2022). PMID 35393517. (original). [link to RRC of NBRP]
RDB19671pCAG-Dcst2-3xHAExpression vector of mouse Dcst2-3xHA under the CAG promoter.Noda, T., Sperm membrane proteins DCST1 and DCST2 are required for sperm-egg interaction in mice and fish. Commun. Biol. 5 (1): 332 (2022). PMID 35393517. (original). [link to RRC of NBRP]
RDB19672pCAG-Izumo1-1D4Expression vector of mouse Izumo1-1D4 under the CAG promoter.Noda, T., Sperm membrane proteins DCST1 and DCST2 are required for sperm-egg interaction in mice and fish. Commun. Biol. 5 (1): 332 (2022). PMID 35393517. (original). [link to RRC of NBRP]
RDB19673pClgn1.1_Dcst1-3xHAExpression vector of mouse Dcst1-3xHA under the mouse Clgn promoter.Noda, T., Sperm membrane proteins DCST1 and DCST2 are required for sperm-egg interaction in mice and fish. Commun. Biol. 5 (1): 332 (2022). PMID 35393517. (original). [link to RRC of NBRP]
RDB19674pClgn1.1_Dcst2-3xHAExpression vector of mouse Dcst2-3xHA under the mouse Clgn promoter.Noda, T., Sperm membrane proteins DCST1 and DCST2 are required for sperm-egg interaction in mice and fish. Commun. Biol. 5 (1): 332 (2022). PMID 35393517. (original). [link to RRC of NBRP]
RDB19972pCAG1.1-Tsks/1D4Expression vector of mouse TSKS (variant X6). 1D4 tag at C-terminus.Shimada, K., TSKS localizes to nuage in spermatids and regulates cytoplasmic elimination during spermiation. Proc. Natl. Acad. Sci. U.S.A. 120 (11): e2221762120 (2023). PMID 36881620. (original). [link to RRC of NBRP]
RDB19973pCAG1.1-Myc/TSSK1Expression vector of mouse TSSK1. Myc-tag at N-terminus.Shimada, K., TSKS localizes to nuage in spermatids and regulates cytoplasmic elimination during spermiation. Proc. Natl. Acad. Sci. U.S.A. 120 (11): e2221762120 (2023). PMID 36881620. (original). [link to RRC of NBRP]
RDB19974pCAG1.1-TSSK2/3xFLAGExpression vector of mouse TSSK2. 3xFLAG tag at C-terminus.Shimada, K., TSKS localizes to nuage in spermatids and regulates cytoplasmic elimination during spermiation. Proc. Natl. Acad. Sci. U.S.A. 120 (11): e2221762120 (2023). PMID 36881620. (original). [link to RRC of NBRP]
RDB19975pCAG1.1-HA/Ppp1cc2Expression vector of mouse PPP1CC2. HA tag at N-terminus.Shimada, K., TSKS localizes to nuage in spermatids and regulates cytoplasmic elimination during spermiation. Proc. Natl. Acad. Sci. U.S.A. 120 (11): e2221762120 (2023). PMID 36881620. (original). [link to RRC of NBRP]
RDB19976pCAG-1700029I15Rik-3×FLAG Expression vector of mouse 1700029I15Rik. 3XFLAG tag at C-terminus.Lu, Y., 1700029I15Rik orchestrates the biosynthesis of acrosomal membrane proteins required for sperm-egg interaction. Proc. Natl. Acad. Sci. U.S.A. 120 (8): e2207263120 (2023). PMID 36787362. (original). [link to RRC of NBRP]

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2024.02.15

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