Resource data sheet
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pCAG-1700029I15Rik-3×FLAG (#RDB19976)

Expression vector of mouse 1700029I15Rik. 3XFLAG tag at C-terminus.

Clone info. Expression vector of mouse 1700029I15Rik. 3XFLAG tag at C-terminus.
Vector backbone pCAG (plasmid)
Selectable markers Ampicillin (E. coli)
Growth conditions LB+Amp, 37oC
Gene/insert name mouse Frey1 cDNA; synthetic 3xFLAG-tag.
Depositor|Developer Ikawa, Masahito | Lu, Yonggang |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(Proc Natl Acad Sci U S A. 120(8): e2207263120, 2023)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用すること（Proc Natl Acad Sci U S A. 120(8): e2207263120, 2023）。利用者が非営利目的の教育・研究以外の目的に用いる場合は、大阪大学と別途MTAを締結すること。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB19976 pCAG-1700029I15Rik-3×FLAG Under QC test. Please contact us. DNA solution

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How to cite this biological resource

Materials & Methods section:

The pCAG-1700029I15Rik-3×FLAG was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19976).

Reference section:

Lu, Y., Shimada, K., Tang, S., Zhang, J., Ogawa, Y., Noda, T., Shibuya, H., Ikawa, M., 1700029I15Rik orchestrates the biosynthesis of acrosomal membrane proteins required for sperm-egg interaction. Proc. Natl. Acad. Sci. U.S.A. 120 (8): e2207263120 (2023). PMID 36787362. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Lu, Y., 1700029I15Rik orchestrates the biosynthesis of acrosomal membrane proteins required for sperm-egg interaction. Proc. Natl. Acad. Sci. U.S.A. 120 (8): e2207263120 (2023). PMID 36787362. [link to RRC of NBRP]