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pCAG-MCS-TurboID-3xFLAG

CAG promoter-driven expression of TurboID-3xFLAG-fused protein (C terminus).

Catalog number RDB19660
Resource name pCAG-MCS-TurboID-3xFLAG
Clone info. CAG promoter-driven expression of TurboID-3xFLAG-fused protein (C terminus). TurboID is a proximity labeling tool for proteomic analysis and is derived from the E. coli.
Vector backbone pCAG1.1 (plasmid)
Size of vector backbone 5.2 kb
Selectable markers Ampicillin
Gene/insert name Escherichia coli biotin protein ligase (TurboID); synthetic FLAG-tag.
Depositor|Developer Ikawa, Masahito | Oura, Seiya |
Other clones in our bank
External Database
Escherichia coli biotin protein ligase (TurboID)
          Reference sequence
            
           
          Sequence (full) RDB19660hts01.seqcheck assembled from experimentally sequenced data
          Test sheet RDB19660_B2Gjp1-1.pdf 

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Oura, S. et al. Sci. Rep. 12 (1): 22198 (2022))
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Oura, S. et al. Sci. Rep. 12 (1): 22198 (2022))。
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          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB19660 pCAG-MCS-TurboID-3xFLAG DNA solution

          How to cite this biological resource

          Materials & Methods section:

          The pCAG-MCS-TurboID-3xFLAG was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19660).

          Reference section:

          Oura, S., Ninomiya, A., Sugihara, F., Matzuk, M.M., Ikawa, M., Proximity-dependent biotin labeling in testicular germ cells identified TESMIN-associated proteins. Sci. Rep. 12 (1): 22198 (2022). PMID 36564444. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

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          References

          Original, user report and related articles

          original Oura, S., Proximity-dependent biotin labeling in testicular germ cells identified TESMIN-associated proteins. Sci. Rep. 12 (1): 22198 (2022). PMID 36564444. [link to RRC of NBRP]

          2023.11.13

          GNP_filter3_RDBDEP_html_231027.pl