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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAG_mRsph4a-PA (#RDB17450)

Expression vector of mouse Rsph4a.

Alternative name mouse Rsph4a on PA tagged pCAG vector
Clone info. Expression vector of mouse radial spoke head 4 homolog A (Rsph4a) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal.
Comment Rsph4a was amplified from mouse testis cDNA. The PA tag is a dodecapeptide (GVAMPGAEDDVV) that is recognized by this high-affinity antibody. Expression was confirmed by the depositor with western blotting.
Vector backbone pCAGGS (plasmid)
Size of vector backbone 4.8 kb
Selectable markers Ampicillin
Gene/insert name mouse Rsph4a cDNA
Depositor|Developer Ikawa, Masahito | Miyata, haruhiko |
 
Sequence (full) RDB17450zzk02.seq provided by the depositor

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Abbasi, F et al. J. Cell Sci. 131(19) jcs221648, 2018)
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Abbasi, F et al. J. Cell Sci. 131(19) jcs221648, 2018)。

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RDB17450 pCAG_mRsph4a-PA DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCAG_mRsph4a-PA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17450).

Reference section:

Abbasi, F., Miyata, H., Shimada, K., Morohoshi, A., Nozawa, K., Matsumura, T., Xu, Z., Pratiwi, P., Ikawa, M., RSPH6A is required for sperm flagellum formation and male fertility in mice. J. Cell Sci. 131 (19). pii: jcs221648 (2018). PMID 30185526. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17450_A9G9p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 (Pr0164)
Region: insert 5'
Sequence file: RDB17450_A9G9a.seq check
>D06337A2_A9Gg_1_pAxCA_F1_E01_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNCCTT CTTCTTTTTC CNNCNGCTCC TGGGCAACGT GCTGGTNATT
   61 GTGCTGTCTC ATCATTTTGG CAAATCTAGA ATCGATGCGG CCGCAGTCGA CAAGCTTGCC
  121 GCCATGGAAA ACTCTACCTC TCTGAAACAA GAAAAGGAAA ATCAAGAGCC AGGGGAAGCA
  181 GAGAGATTAT GGCAAGGAGA GTCAGACGTT TCTCCCCAGG AGCCTGGTCC CCCATCACCT
  241 GAGTACCGGG AAGAGGAGCA AAGGACAGAC ACCGAACCCG CACCCAGAAT GAGCCCCTCT
  301 TGGAGCCACC AGTCTAGAGT CAGCCTGTCT ACGGGAGACC TCACCGCTGG ACCAGAAGTG
  361 TCATCCTCCC CTCCCCCTCC TCCTCTGCAG TTTCATTCCA CTCCTCTCAA CACTGAAACT
  421 ACTCAGGATC CTGTAGCTGC ATCACCCACA GAGAAGACCG CTAATGGAAT CGCTGACACC
  481 GGGACACCTT ATTCTGATCC TTGGGAATCA TCTTCTGCTG CAAAGCAATC AACTTCTCAC
  541 TACACAAGCC ACGCCGAGGA AAGTACTTTC CCGCAGTCTC AGACACCCCA ACCTGACCTC
  601 TGTGGGCTAA GGGATGCCAG CAGGAACAAG TCCAAGCACA AAGGGCTGAN ATTTGACCTT
  661 TTACAAGAGG AANGCTCAGA CAGTAACTGT GACCCAGACC AGCCTGANGT TGGAGCCAGT
  721 GAAGCAGCCC AGANTATGCT TGANGTGGNC ATTCAGAATG NAAAAGCCTA CCTGCTGANT
  781 ACCAGCAGCA AGTCTGGCTT AAATCTATAT GANNATCTTT CTAAAGTGTT GANCAAGATA
  841 CTAGATGANC GTCCTGCANA NGCTGTTGAN NTCANTGAAA ATATAAGCCN AGANGTGAAA
  901 NTGGNNCATT TTAATAAAAA ATTANATACN CTCNACANNG AANNNGANAN GNNNNCNNCA
  961 TANGAAATAN CNNANNNNNA AANNNNNNTT TTTTNNNNNN GGNNNNTTNG NANNNNGNTN
1021 ANNCNNNNNN NNNN
//
Primer: pCA-bgter (Pr0066)
Region: PA tag, insert 3'
Sequence file: RDB17450_A9G9b.seq check
>D06337A2_A9Gg_1_pCA-bgter_F01_16_ABI24.ab1
    1 NNNNNNNNNN CNNNNNNTTT TGNNNNANGG ANAAGNNCTC NGTGGTATTT GTGAGCCAGG
   61 GCATTGGCCA CACCAGCCAC CACCTTCTGA TAGGCAGCCT GCACCTGAGG AGTCTCGAGC
  121 TAGGCAGGCG CCACTTGGCT GGTCTCTGTG CCCCCGCCCC CGATCACCAC ATCATCTTCG
  181 GCACCTGGCA TGGCAACGCC GATATCGAAT TCGTCCTCAT CGTCCTCATC TTCATCTTCA
  241 GTTCCTTCGT TCTCCTCAGT TGAAAGTGCA ACTGGCTCTT GTGTCATCCT GAAAGCCTGT
  301 TCCTCCTCCA CACTGGGATC ATTCATTTCT GTAATTTCTG GTCCACTGGG ATATTCTTGA
  361 TAAACTGGTG GTGGGCTTGG GGGTGTGTAA TTCTCCACAC AGTACTTATG ACCCCAGCCT
  421 ATGTAGAAGT TTTCAAACTT CTTGCCATTG GAAAAAGCAT ATGCTCCAGG CCAAAGATTG
  481 GAGCGAAGGA CAGCAATGGC ATACTGTGGA ATGAGATTTG AAGACAGCTG TGTTGTCCAA
  541 GAGGGTATAT TCTGAATCCC CAAATCTTCA GATATTGGTG TCAAAAGAGG AGGTCCGACT
  601 TCCTGCTCTA TGTAGTCAGG TTCTTCCCCC TTTTCTTCAT CTTCTTCCTC TTCCTCTTCT
  661 TCATCTTCAT CTTTTTGTAT AGGGTTGAAC CAATTACAGC GACCCTGAGG AANANTATAC
  721 TGCACGTGAT GAACCCAATT GGATAGAGAC TCNACCAAGT CAATCACTTG GATGCCTTCA
  781 AAGTCAGGGT TTTCCTCATA GCTGTCTCGA CCACCTTCCA CCTCTTCTTC CTCTCCTTCC
  841 TCTTCACCAA ACTGATANAA TCCTANAGGA NTGACATGNG TCCNNGCTGA ANTGNGGGCA
  901 ATTTGTGCTC TTAANNNNNT GNTCNNNTTT CCTGGGAANG GNGGNTAGCT TANGANANCN
961 NNNTCTANNC NNNNNNNGAA AAANTNNNNG ATTTTTNNNT NNNNNNNNNN
//
Primer: pUC-CAPbs_up_F (Pr0536)
Region: lac pro, lacO, T3 pro, CMV en, CAG pro
Sequence file: RDB17450_A9G9c.seq check
>D06337A2_A9Gg_1_pUC-CAPbs_up_F_G01_19_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNCNCNNNNN NNNTAATGTG AGTTAGCTCN CTCNTTAGGC
   61 ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA
  121 ACAATTTCAC ACAGGAAACA GCTATGACCA TGATTACGCC AAGCGCGCAA TTAACCCTCA
  181 CTAAAGGGAA CAAAAGCTGG AGCTCATCGA TACTCGACAT TGATTATTGA CTAGTTATTA
  241 ATAGTAATCA ATTACGGGGT CATTAGTTCA TAGCCCATAT ATGGAGTTCC GCGTTACATA
  301 ACTTACGGTA AATGGCCCGC CTGGCTGACC GCCCAACGAC CCCCGCCCAT TGACGTCAAT
  361 AATGACGTAT GTTCCCATAG TAACGCCAAT AGGGACTTTC CATTGACGTC AATGGGTGGA
  421 GTATTTACGG TAAACTGCCC ACTTGGCAGT ACATCAAGTG TATCATATGC CAAGTACGCC
  481 CCCTATTGAC GTCAATGACG GTAAATGGCC CGCCTGGCAT TATGCCCAGT ACATGACCTT
  541 ATGGGACTTT CCTACTTGGC AGTACATCTA CGTATTAGTC ATCGCTATTA CCATGGTCGA
  601 GGTGAGCCCC ACGTTCTGCT TCACTCTCCC CATCTCCCCC CCCTCCCCAC CCCCAATTTT
  661 GTATTTATTT ATTTTTTAAT TATTTTGTGC AGCGATGGGG GCNNNNGGGG GGNNNNNNNN
721 NN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Abbasi, F., RSPH6A is required for sperm flagellum formation and male fertility in mice. J. Cell Sci. 131 (19). pii: jcs221648 (2018). PMID 30185526. [link to RRC of NBRP]