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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAG_mRsph4a-PA

Expression vector of mouse Rsph4a.

Catalog number RDB17450
Resource name pCAG_mRsph4a-PA
Alternative name mouse Rsph4a on PA tagged pCAG vector
Clone info. Expression vector of mouse radial spoke head 4 homolog A (Rsph4a) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal.
Comment Rsph4a was amplified from mouse testis cDNA. The PA tag is a dodecapeptide (GVAMPGAEDDVV) that is recognized by this high-affinity antibody. Expression was confirmed by the depositor with western blotting.
Vector backbone pCAGGS (plasmid)
Size of vector backbone 4.8 kb
Selectable markers Ampicillin
Gene/insert name mouse Rsph4a cDNA
Depositor|Developer Ikawa, Masahito | Miyata, Haruhiko |
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External Database
mouse Rsph4a

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Abbasi, F et al. J. Cell Sci. 131(19) jcs221648, 2018)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Abbasi, F et al. J. Cell Sci. 131(19) jcs221648, 2018)。
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          RDB17450 pCAG_mRsph4a-PA DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence (full) RDB17450zzk02.seq provided by the depositor

          Original reference

          original Abbasi, F., RSPH6A is required for sperm flagellum formation and male fertility in mice. J. Cell Sci. 131 (19). pii: jcs221648 (2018). PMID 30185526.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB17450_A9G9p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pAxCA_F1
          Region: insert 5'
          Sequence file: RDB17450_A9G9a.seq check
          >D06337A2_A9Gg_1_pAxCA_F1_E01_13_ABI24.ab1
              1 NNNNNNNNNN NNNNNNCCTT CTTCTTTTTC CNNCNGCTCC TGGGCAACGT GCTGGTNATT
             61 GTGCTGTCTC ATCATTTTGG CAAATCTAGA ATCGATGCGG CCGCAGTCGA CAAGCTTGCC
            121 GCCATGGAAA ACTCTACCTC TCTGAAACAA GAAAAGGAAA ATCAAGAGCC AGGGGAAGCA
            181 GAGAGATTAT GGCAAGGAGA GTCAGACGTT TCTCCCCAGG AGCCTGGTCC CCCATCACCT
            241 GAGTACCGGG AAGAGGAGCA AAGGACAGAC ACCGAACCCG CACCCAGAAT GAGCCCCTCT
            301 TGGAGCCACC AGTCTAGAGT CAGCCTGTCT ACGGGAGACC TCACCGCTGG ACCAGAAGTG
            361 TCATCCTCCC CTCCCCCTCC TCCTCTGCAG TTTCATTCCA CTCCTCTCAA CACTGAAACT
            421 ACTCAGGATC CTGTAGCTGC ATCACCCACA GAGAAGACCG CTAATGGAAT CGCTGACACC
            481 GGGACACCTT ATTCTGATCC TTGGGAATCA TCTTCTGCTG CAAAGCAATC AACTTCTCAC
            541 TACACAAGCC ACGCCGAGGA AAGTACTTTC CCGCAGTCTC AGACACCCCA ACCTGACCTC
            601 TGTGGGCTAA GGGATGCCAG CAGGAACAAG TCCAAGCACA AAGGGCTGAN ATTTGACCTT
            661 TTACAAGAGG AANGCTCAGA CAGTAACTGT GACCCAGACC AGCCTGANGT TGGAGCCAGT
            721 GAAGCAGCCC AGANTATGCT TGANGTGGNC ATTCAGAATG NAAAAGCCTA CCTGCTGANT
            781 ACCAGCAGCA AGTCTGGCTT AAATCTATAT GANNATCTTT CTAAAGTGTT GANCAAGATA
            841 CTAGATGANC GTCCTGCANA NGCTGTTGAN NTCANTGAAA ATATAAGCCN AGANGTGAAA
            901 NTGGNNCATT TTAATAAAAA ATTANATACN CTCNACANNG AANNNGANAN GNNNNCNNCA
            961 TANGAAATAN CNNANNNNNA AANNNNNNTT TTTTNNNNNN GGNNNNTTNG NANNNNGNTN
          1021 ANNCNNNNNN NNNN
          //
          Primer: pCA-bgter
          Region: PA tag, insert 3'
          Sequence file: RDB17450_A9G9b.seq check
          >D06337A2_A9Gg_1_pCA-bgter_F01_16_ABI24.ab1
              1 NNNNNNNNNN CNNNNNNTTT TGNNNNANGG ANAAGNNCTC NGTGGTATTT GTGAGCCAGG
             61 GCATTGGCCA CACCAGCCAC CACCTTCTGA TAGGCAGCCT GCACCTGAGG AGTCTCGAGC
            121 TAGGCAGGCG CCACTTGGCT GGTCTCTGTG CCCCCGCCCC CGATCACCAC ATCATCTTCG
            181 GCACCTGGCA TGGCAACGCC GATATCGAAT TCGTCCTCAT CGTCCTCATC TTCATCTTCA
            241 GTTCCTTCGT TCTCCTCAGT TGAAAGTGCA ACTGGCTCTT GTGTCATCCT GAAAGCCTGT
            301 TCCTCCTCCA CACTGGGATC ATTCATTTCT GTAATTTCTG GTCCACTGGG ATATTCTTGA
            361 TAAACTGGTG GTGGGCTTGG GGGTGTGTAA TTCTCCACAC AGTACTTATG ACCCCAGCCT
            421 ATGTAGAAGT TTTCAAACTT CTTGCCATTG GAAAAAGCAT ATGCTCCAGG CCAAAGATTG
            481 GAGCGAAGGA CAGCAATGGC ATACTGTGGA ATGAGATTTG AAGACAGCTG TGTTGTCCAA
            541 GAGGGTATAT TCTGAATCCC CAAATCTTCA GATATTGGTG TCAAAAGAGG AGGTCCGACT
            601 TCCTGCTCTA TGTAGTCAGG TTCTTCCCCC TTTTCTTCAT CTTCTTCCTC TTCCTCTTCT
            661 TCATCTTCAT CTTTTTGTAT AGGGTTGAAC CAATTACAGC GACCCTGAGG AANANTATAC
            721 TGCACGTGAT GAACCCAATT GGATAGAGAC TCNACCAAGT CAATCACTTG GATGCCTTCA
            781 AAGTCAGGGT TTTCCTCATA GCTGTCTCGA CCACCTTCCA CCTCTTCTTC CTCTCCTTCC
            841 TCTTCACCAA ACTGATANAA TCCTANAGGA NTGACATGNG TCCNNGCTGA ANTGNGGGCA
            901 ATTTGTGCTC TTAANNNNNT GNTCNNNTTT CCTGGGAANG GNGGNTAGCT TANGANANCN
          961 NNNTCTANNC NNNNNNNGAA AAANTNNNNG ATTTTTNNNT NNNNNNNNNN
          //
          Primer: pUC-CAPbs_up_F
          Region: lac pro, lacO, T3 pro, CMV en, CAG pro
          Sequence file: RDB17450_A9G9c.seq check
          >D06337A2_A9Gg_1_pUC-CAPbs_up_F_G01_19_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNCNCNNNNN NNNTAATGTG AGTTAGCTCN CTCNTTAGGC
             61 ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA
            121 ACAATTTCAC ACAGGAAACA GCTATGACCA TGATTACGCC AAGCGCGCAA TTAACCCTCA
            181 CTAAAGGGAA CAAAAGCTGG AGCTCATCGA TACTCGACAT TGATTATTGA CTAGTTATTA
            241 ATAGTAATCA ATTACGGGGT CATTAGTTCA TAGCCCATAT ATGGAGTTCC GCGTTACATA
            301 ACTTACGGTA AATGGCCCGC CTGGCTGACC GCCCAACGAC CCCCGCCCAT TGACGTCAAT
            361 AATGACGTAT GTTCCCATAG TAACGCCAAT AGGGACTTTC CATTGACGTC AATGGGTGGA
            421 GTATTTACGG TAAACTGCCC ACTTGGCAGT ACATCAAGTG TATCATATGC CAAGTACGCC
            481 CCCTATTGAC GTCAATGACG GTAAATGGCC CGCCTGGCAT TATGCCCAGT ACATGACCTT
            541 ATGGGACTTT CCTACTTGGC AGTACATCTA CGTATTAGTC ATCGCTATTA CCATGGTCGA
            601 GGTGAGCCCC ACGTTCTGCT TCACTCTCCC CATCTCCCCC CCCTCCCCAC CCCCAATTTT
            661 GTATTTATTT ATTTTTTAAT TATTTTGTGC AGCGATGGGG GCNNNNGGGG GGNNNNNNNN
          721 NN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Abbasi, F., RSPH6A is required for sperm flagellum formation and male fertility in mice. J. Cell Sci. 131 (19). pii: jcs221648 (2018). PMID 30185526.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl