Resource data sheet
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pCAG1.1-Armc12 delta C/FLAG (#RDB19466)

Expression vector of C-terminal deleted mouse Armc12 tagged with FLAG at C-terminus.

Clone info. Expression vector of C-terminal deleted mouse armadillo repeat containing 12 (Armc12) tagged with FLAG at C-terminus. See Fig.4D of Shimada, K. et al. Proc. Natl. Acad. Sci. USA. 118 (6): e2018355118, 2021.
Comment Expression was confirmed by the depositor with immunoblotting, immunofluorecence.
Vector backbone pCAG1.1 (plasmid)
Selectable markers Ampicillin
Gene/insert name mouse Armc12 cDNA
Depositor|Developer Ikawa, Masahito | Shimada, Keisuke |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Shimada, K. et al., Proc. Natl. Acad. Sci. USA. 118 (6): e2018355118, 2021). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Shimada, K. et al., Proc. Natl. Acad. Sci. USA. 118 (6): e2018355118, 2021)。利用者が非営利目的の教育・研究以外の目的に用いる場合は、大阪大学と別途MTAを締結すること。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB19466 pCAG1.1-Armc12 delta C/FLAG Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

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How to cite this biological resource

Materials & Methods section:

The pCAG1.1-Armc12 delta C/FLAG was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19466).

Reference section:

Shimada, K., Park, S., Miyata, H., Yu, Z., Morohoshi, A., Oura, S., Matzuk, M.M., Ikawa, M., ARMC12 regulates spatiotemporal mitochondrial dynamics during spermiogenesis and is required for male fertility. Proc Natl Acad Sci U S A. 2021 Feb 9;118(6):e2018355118 (2021). PMID 33536340. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Shimada, K., ARMC12 regulates spatiotemporal mitochondrial dynamics during spermiogenesis and is required for male fertility. Proc Natl Acad Sci U S A. 2021 Feb 9;118(6):e2018355118 (2021). PMID 33536340. [link to RRC of NBRP]