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pCAG-3xFLAG-TurboID-MCS (#RDB19658)

CAG promoter-driven expression of 3xFLAG-TurboID-fused protein (N terminus).


Drawn by SnapGene® software
Sequence (full) RDB19658hts01.seq
Publication Oura, S., Sci. Rep. 12 (1): 22198 (2022). [link to RRC of NBRP]
Test sheet RDB19658_B2Gjp1-1.pdf 
Assembled from experimentally sequenced data.
 
Clone info. CAG promoter-driven expression of 3xFLAG-TurboID-fused protein (N terminus). TurboID is a proximity labeling tool for proteomic analysis and is derived from the E. coli.
Vector backbone pCAG1.1 (plasmid)
Size of vector backbone 5.2 kb
Selectable markers Ampicillin
Gene/insert name Escherichia coli biotin protein ligase (TurboID); synthetic FLAG-tag.
Depositor|Developer Ikawa, Masahito | Oura, Seiya |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Oura, S. et al. Sci. Rep. 12 (1): 22198 (2022))
提供案内 (日本国内) [open/close]

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提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Oura, S. et al. Sci. Rep. 12 (1): 22198 (2022))。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19658 pCAG-3xFLAG-TurboID-MCS DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pCAG-3xFLAG-TurboID-MCS was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19658).

Reference section:

Oura, S., Ninomiya, A., Sugihara, F., Matzuk, M.M., Ikawa, M., Proximity-dependent biotin labeling in testicular germ cells identified TESMIN-associated proteins. Sci. Rep. 12 (1): 22198 (2022). PMID 36564444. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Oura, S., Proximity-dependent biotin labeling in testicular germ cells identified TESMIN-associated proteins. Sci. Rep. 12 (1): 22198 (2022). PMID 36564444. [link to RRC of NBRP]