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pCAG-hPPP3CC-FLAG

Expression vector of human PPP3CC with FLAG-tag.

Catalog number RDB19408
Resource name pCAG-hPPP3CC-FLAG
Clone info. Expression vector of human protein phosphatase 3, catalytic subunit, gamma isoform (PPP3CC) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.
Comment Expression was confirmed by the depositor with western blotting.
Vector backbone pCAGGS (plasmid)
Size of vector backbone 5.2 kb
Selectable markers Ampicillin
Gene/insert name human PPP3CC cDNA
Depositor|Developer Ikawa, Masahito |
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External Database
human PPP3CC

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Miyata, H. et al., Science, 350 (6259): 442-445, 2015) RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
          Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
          Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Miyata, H. et al., Science, 350 (6259): 442-445, 2015)。利用者が非営利目的の教育・研究以外の目的に用いる場合は、大阪大学と別途MTAを締結すること。
          付加的提供条件 CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB19408 pCAG-hPPP3CC-FLAG Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Electronic file

          Original reference

          original Miyata, H., Sperm calcineurin inhibition prevents mouse fertility with implications for male contraceptive. Science 350 (6259): 442-445 (2015). PMID 26429887.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Miyata, H., Sperm calcineurin inhibition prevents mouse fertility with implications for male contraceptive. Science 350 (6259): 442-445 (2015). PMID 26429887.

          2022.05.16

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