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pCAG-EGxxFP

Validation plasmid for the effectiveness of guide RNA in CRISPR/Cas9 system.

Catalog number RDB13532
Resource name pCAG-EGxxFP
Clone info. Validation plasmid for the effectiveness of guide RNA in CRISPR/Cas9 system.
Comment For validation of sgRNA on double strand breaks by observation of green fluorescence. MCS: BamHI, NheI, PstI, SalI, EcoRI, and EcoRV.
Vector backbone pCAGGS (Plasmid)
Selectable markers Amp^r
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Ikawa, Masahito |
Other clones in our bank
External Database
Aequorea victoria GFP
            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Mashiko, D., Sci. Rep. 3: 3355 (2013)) The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five (5) years must obtain a written consent from the DEPOSITOR again.
            Additional terms and conditions:
            Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
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            MTA, for use for not-for-profit academic purpose [Word]
            MTA, for use for for-profit purpose [Word]
            Approval form by depositor [Word]
            Please visit Information of Request for Distribution.[link] 
            Remarks ((Additional form)) Approval Form(FormD)
            Please obtain written approval of the depositor.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は事前に寄託者の提供承諾書を得る。非営利機関が非営利目的の教育・研究用に用いる場合以外は、大阪大学と別途MTAを締結すること。研究成果の公表にあたって寄託者の指定する文献を引用する (Mashiko, D., Sci. Rep. 3: 3355 (2013))。5年経過後も使用を希望するときは改めて寄託者から承諾を得るものとする。
            付加的提供条件：
            CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
            提供依頼
            提供依頼書 [Word]
            提供同意書 (MTA, 非営利学術目的用)[Word]
            提供同意書 (MTA, 営利目的用)[Word]
            寄託者による提供承諾書 [Word]
            手続きの概要は、「提供申込みについて[link]」をご覧ください。
            備考 【追加提供依頼書】承諾書(FormD)
            寄託者の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB13532 pCAG-EGxxFP DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pCAG-EGxxFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13532).

            Reference section:

            Mashiko, D., Fujihara, Y., Satouh, Y., Miyata, H., Isotani, A., Ikawa, M., Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA. Sci. Rep. 3: 3355 (2013). PMID 24284873. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)

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            Featured content Genome Editing (English text)
            Featured content Genome Editing (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB18565_B0Iep1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pCAG-F (Pr0328)
            Region: CAG pro,Ensert 5'(EGxxFP-N)
            Sequence file: RDB18565_B0Iea.seq check
            >13532_18565_B0Ie_2_pCAG-F_H04_22_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNANGGNGG GGTTCGGCTT CTGGCGNGTG
               61 NNCGGCGGCT CTANCCTCTG CTAACCATGT TCATGCCTTC TTCTTTTTCC TACAGCTCCT
              121 GGGCAACGTG CTGGTTATTG TGCTGTCTCA TCATTTTGGC AAATCTAGAG CCGCCATGGT
              181 GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA
              241 CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA
              301 GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT
              361 GACCACCCTG ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA
              421 CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA
              481 GGACGACGGC AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA
              541 CCGCATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT
              601 GGAGTACAAC TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT
              661 CAAGGTGAAC TTCAAGATCC GCCACAACAT CGAGGANGGC AGCGTGCAGC TCGCCGACCA
              721 CTACCAGCAG AACACCCCCA TCGGCGANNG CCNCGTGCTG CTGCCCGACA ACCACTGNNG
              781 NNCCGCTAGC CTGCNNNCGA CGAATTCNNT ATCGGCNAGC TGANCCTGAN NTTCATCTGC
              841 ACCANNGGCA ANCTGCCCGN GCCCNNNNNC ACCNNNNNGA NCACCCNNNN NNANNNNNNG
            901 CNNNNCTTCA NNNNNNNNNC NNNNNNNNNN
            //
            Primer: pCAGGS_R1 (Pr0614)
            Region: insert 3'(EGxxFP-C)
            Sequence file: RDB18565_B0Ieb.seq check
            >13532_18565_B0Ie_2_pCAGGS_R1_H05_23_ABI24.ab1
                1 NNNNNNNNNN ANNCNCTATT GCATGNNNNA AATTTCCTTT ATTAGCCAGA AGTCAGATGC
               61 TCAAGGGGCT TCATGATGTC CCCATAATTT TTGGCAGAGG GAAAAAGATC TCAGTGGTAT
              121 TTGTGAGCCA GGGCATTGGC CACACCAGCC ACCACCTTCT GATAGGCAGC CTGCACCTGA
              181 GGNGTCTCGA GTTACTTGTA CAGCTCGTCC ATGCCGAGAG TGATCCCGGC GGCGGTCACG
              241 AACTCCAGCA GGACCATGTG ATCGCGCTTC TCGTTGGGGT CTTTGCTCAG GGCGGACTGG
              301 GTGCTCAGGT AGTGGTTGTC GGGCAGCAGC ACGGGGCCGT CGCCGATGGG GGTGTTCTGC
              361 TGGTAGTGGT CGGCGAGCTG CACGCTGCCG TCCTCGATGT TGTGGCGGAT CTTGAAGTTC
              421 ACCTTGATGC CGTTCTTCTG CTTGTCGGCC ATGATATAGA CGTTGTGGCT GTTGTAGTTG
              481 TACTCCAGCT TGTGCCCCAG GATGTTGCCG TCCTCCTTGA AGTCGATGCC CTTCAGCTCG
              541 ATGCGGTTCA CCAGGGTGTC GCCCTCGAAC TTCACCTCGG CGCGGGTCTT GTAGTTGCCG
              601 TCGTCCTTGA AGAAGANGGT GCGCTCCTGG ACGTAGCCTT CGGGCATGGC GGACTTGAAG
              661 AAGTCGTGCT GCTTCATGTG GTCGGGGTAG CGGCTGAAGC ACTGCACGCC GTANGTCANG
              721 GTGGTCACGA NGGTGGGCCA GGGCACGGGC AGCTTGCCGG NGGTGCANAT GAACTTCNNG
              781 GTCAGCTTGC CGANATCGAA TTCGTCNANC TGCNNNCTAN CGGNNCCTCA NNGGTTGTCN
              841 GGCAGCAGCA CGGGGNCGTC NCCNANGGGG NNNTTCTGCT GGNNNNNNNC GGNNNNCTNC
            901 NNNCTNNNNN NNNNN
            //
            Primer: EGFP-C-rev (Pr0026)
            Region: insert mid(EGxxFP-C,MCS,EGxxFP-N)
            Sequence file: RDB18565_B0Iec.seq check
            >13532_18565_B0Ie_2_EGFP-C-rev_H06_24_ABI24.ab1
                1 NNNNNNNNNN GNNNNTNNCN NNNNGNGGNN NNGGNGCTCA GGTAGTGGTT GTCGGGCAGC
               61 AGCACGGGGC CGTCGCCGAT GGGGGTGTTC TGCTGGTAGT GGTCGGCGAG CTGCACGCTG
              121 CCGTCCTCGA TGTTGTGGCG GATCTTGAAG TTCACCTTGA TGCCGTTCTT CTGCTTGTCG
              181 GCCATGATAT AGACGTTGTG GCTGTTGTAG TTGTACTCCA GCTTGTGCCC CAGGATGTTG
              241 CCGTCCTCCT TGAAGTCGAT GCCCTTCAGC TCGATGCGGT TCACCAGGGT GTCGCCCTCG
              301 AACTTCACCT CGGCGCGGGT CTTGTAGTTG CCGTCGTCCT TGAAGAAGAT GGTGCGCTCC
              361 TGGACGTAGC CTTCGGGCAT GGCGGACTTG AAGAAGTCGT GCTGCTTCAT GTGGTCGGGG
              421 TAGCGGCTGA AGCACTGCAC GCCGTAGGTC AGGGTGGTCA CGAGGGTGGG CCAGGGCACG
              481 GGCAGCTTGC CGGTGGTGCA GATGAACTTC AGGGTCAGCT TGCCGATATC GAATTCGTCG
              541 ACCTGCAGGC TAGCGGATCC TCAGTGGTTG TCGGGCAGCA GCACGGGGCC GTCGCCGATG
              601 GGGGTGTTCT GCTGGTAGTG GTCGGCGAGC TGCACGCTGC CGTCCTCGAT GTTGTGGCGG
              661 ATCTTGAAGT TCACCTTGAT GCCGTTCTTC TGCTTGTCGG CCATGATATA GACGTTGTGG
              721 CTGTTGTAGT TGTACTCCAG CTTGTGCCCC ANGNTGTTGC CGTCCTCCTT NAAGTCGATG
              781 CCCTTCANCT CGATGCGGTT CACCAGGGNG TCGCCCTCNA ACTTCACCTC NGCGCNGGNC
              841 TTGNANTTGC CNNCNNCCTN NAANAAGANN GNGCGCTCCN NNNNNNANCN NTCNGGNCAT
            901 NGNNGANTNN NNNNNNNCNN NCNNNTNNNN NNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            original Mashiko, D., Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA. Sci. Rep. 3: 3355 (2013). PMID 24284873. [link to RRC of NBRP]
            reference Mashiko, D., Feasibility for a large scale mouse mutagenesis by injecting CRISPR/Cas plasmid into zygotes. Dev. Growth Differ. 56 (1): 122-129 (2013). PMID 24372541.

            2023.10.05

            GNP_filter3_RDBDEP_html_231001.pl