Resource data sheet
Prev.

pCAG-EGxxFP

Validation plasmid for the effectiveness of guide RNA in CRISPR/Cas9 system.

Catalog number RDB13532
Resource name pCAG-EGxxFP
Clone info. Validation plasmid for the effectiveness of guide RNA in CRISPR/Cas9 system.
For validation of sgRNA on double strand breaks by observation of green fluorescence. MCS: BamHI, NheI, PstI, SalI, EcoRI, and EcoRV.
Vector backbone pCAGGS (Plasmid)
Selectable markers Amp^r
Gene/insert name Aequorea victoria GFP cDNA
Depositor Ikawa, Masahito |
 
Other clones in our bank Aequorea victoria |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Mashiko, D., Sci. Rep. 3: 3355 (2013)) The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five (5) years must obtain a written consent from the DEPOSITOR again.
(Japanese text [open/close])
Remarks ((Additional form)) Approval Form(FormD), GFP-A
Please use the Exclusive MTA (genome editing) [Word] [pdf]
(Japanese text [open/close])

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13532 pCAG-EGxxFP DNA solution

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13532_A5J2p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 RDB13532_A5J2a.seq
>RDB13532_13532_A5J2_pAxCA_F1_A01_01.ab1
    1 ACCGGACCAC GGGGGGTTCT CTCAAGTTAT GCCTTCTTCT TTTTCCTACA GCTCCTGGGC
   61 AACGTGCTGG TTATTGTGCT GTCTCATCAT TTTGGCAAAT CTAGAGCCGC CATGGTGAGC
  121 AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA
  181 AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG
  241 ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC
  301 ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC
  361 TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC
  421 GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC
  481 ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG
  541 TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG
  601 GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC
  661 CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTGAGGATCC
  721 GCTAGCCTGC AGGTCGACGA ATTCGATATC GGCAAGCTGA CCCTGAAGTT CATCTGCACC
  781 ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGACCTA CGGCGTGCAG
  841 TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC
  901 GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGGAC GACGGCAACT ACAAGACCCG
  961 CGCCGAGGTG AAAGTTCGAG GGCGACACCC CTGGTGAACC GGCATCGAGC TGAAGGGCAT
 1021 CGACTTTCAA GGGAGGACGG CCAACATCTT GGGGGCACAA GGCTGGAGTA CACTACAACA
 1081 GGCACAACCG TCCTATATCC ATGGCCGAAC AGCAAGAAAG AACCGGGCCA ATTCCAGAGG
1141 GTGGTGCACA CTTTCTTCA
//
Primer: pCA-bgter RDB13532_A5J2b.seq
>RDB13532_13532_A5J2_pCA_bgter_C01_07.ab1
    1 GGGAGACCCC GGAAGTCCAA TCATTTTATG GCAGAGGGAA AAAAGATCTC AGTGGTATTT
   61 GTGAGCCAGG GCATTGGCCA CACCAGCCAC CACCTTCTGA TAGGCAGCCT GCACCTGAGG
  121 AGTCTCGAGT TACTTGTACA GCTCGTCCAT GCCGAGAGTG ATCCCGGCGG CGGTCACGAA
  181 CTCCAGCAGG ACCATGTGAT CGCGCTTCTC GTTGGGGTCT TTGCTCAGGG CGGACTGGGT
  241 GCTCAGGTAG TGGTTGTCGG GCAGCAGCAC GGGGCCGTCG CCGATGGGGG TGTTCTGCTG
  301 GTAGTGGTCG GCGAGCTGCA CGCTGCCGTC CTCGATGTTG TGGCGGATCT TGAAGTTCAC
  361 CTTGATGCCG TTCTTCTGCT TGTCGGCCAT GATATAGACG TTGTGGCTGT TGTAGTTGTA
  421 CTCCAGCTTG TGCCCCAGGA TGTTGCCGTC CTCCTTGAAG TCGATGCCCT TCAGCTCGAT
  481 GCGGTTCACC AGGGTGTCGC CCTCGAACTT CACCTCGGCG CGGGTCTTGT AGTTGCCGTC
  541 GTCCTTGAAG AAGATGGTGC GCTCCTGGAC GTAGCCTTCG GGCATGGCGG ACTTGAAGAA
  601 GTCGTGCTGC TTCATGTGGT CGGGGTAGCG GCTGAAGCAC TGCACGCCGT AGGTCAGGGT
  661 GGTCACGAGG GTGGGCCAGG GCACGGGCAG CTTGCCGGTG GTGCAGATGA ACTTCAGGGT
  721 CAGCTTGCCG ATATCGAATT CGTCGACCTG CAGGCTAGCG GATCCTCAGT GGTTGTCGGG
  781 CAGCAGCACG GGGCCGTCGC CGATGGGGGT GTTCTGCTGG TAGTGGTCGG CGAGCTGCAC
  841 GCTGCCGTCC TCGATGTTGT GGCGGATCTT GAAAGTTCAC CTTGATGCCG TTCTTCTGCT
  901 TGTCGGCCAT GATATAGACG TTGTGGCTGT TGTAGTTGTA CTCCAGCTTG TGCCCCAGGA
  961 TGTTGCCGTC CTTCCTTTGA AGTCGATGCC CTTCAGCCTC GAATGCGGGT TCACCCAGGG
 1021 TGTCGCCCTT CGAACTTTCA CCCTCGGGCG CGGTCTTGTA GTTGCCCGTT CGTCCCTTGA
 1081 AGAAGATGGT GCCGCTTTCC TGGAACGTAA GCCCTTCGGA CATGGCGGGC ACTGGAGAGT
1141 CCGTTGCTGC TTCATGTGGC TTCGGTATAA GTCGCTGCAG CT
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

Featured content

Featured content Genome Editing (English text)
Featured content Genome Editing (Japanese text)

References

original Mashiko, D., Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA. Sci. Rep. 3: 3355 (2013). PMID 24284873.
reference Mashiko, D., Feasibility for a large scale mouse mutagenesis by injecting CRISPR/Cas plasmid into zygotes. Dev. Growth Differ. 56 (1): 122-129 (2013). PMID 24372541.

2017.10.19

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