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pX330-Cetn1/sgRNA#1

Expression plasmid targeting mouse Cetn1 gene in CRISPR/Cas9 system.

Catalog number RDB13533
Resource name pX330-Cetn1/sgRNA#1
Clone info. Expression plasmid targeting mouse Cetn1 gene in CRISPR/Cas9 system.
Comment MGI ID, 1347086. sgRNA recognition sequence, GGCAACGTTTGACTTCCTGA.
Vector backbone pX330 (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse centrin 1 genomic DNA
Depositor|Developer Ikawa, Masahito |
Other clones in our bank
External Database
Mouse centrin 1
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Mashiko, D., Sci. Rep. 3: 3355 (2013)) The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
          Additional terms and conditions:
          Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
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          Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
          Approval form by depositor [Word]
          Please visit Information of Request for Distribution.[link] 
          Remarks ((Additional form)) Approval Form(FormD)
          Please obtain written approval of the depositor.
          The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は事前に寄託者の提供承諾書を得る。非営利機関が非営利目的の教育・研究用に用いる場合以外は、大阪大学と別途MTAを締結すること。研究成果の公表にあたって寄託者の指定する文献を引用する（Mashiko, D., Sci. Rep. 3: 3355 (2013)）。5年経過後も使用を希望するときは改めて寄託者から承諾を得るものとする。
          付加的提供条件：
          CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
          提供依頼
          提供依頼書 [Word]
          専用MTA（CRISPR/Cas9内包遺伝子材料専用 非営利学術目的）をお使いください [Word]
          寄託者による提供承諾書 [Word]
          手続きの概要は、「提供申込みについて[link]」をご覧ください。
          備考 【追加提供依頼書】承諾書(FormD)
          寄託者の書面による提供承諾が必要です。
          本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB13533 pX330-Cetn1/sgRNA#1 Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          How to cite this biological resource

          Materials & Methods section:

          The pX330-Cetn1/sgRNA#1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13533).

          Reference section:

          Mashiko, D., Fujihara, Y., Satouh, Y., Miyata, H., Isotani, A., Ikawa, M., Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA. Sci. Rep. 3: 3355 (2013). PMID 24284873. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

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          Featured content Genome Editing (English text)
          Featured content Genome Editing (Japanese text)

          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Mashiko, D., Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA. Sci. Rep. 3: 3355 (2013). PMID 24284873. [link to RRC of NBRP]

          2023.10.05

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