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pX330-Cetn1/sgRNA#1

Expression plasmid targeting mouse Cetn1 gene in CRISPR/Cas9 system.

Catalog number RDB13533
Resource name pX330-Cetn1/sgRNA#1
Clone info. Expression plasmid targeting mouse Cetn1 gene in CRISPR/Cas9 system.
MGI ID, 1347086. sgRNA recognition sequence, GGCAACGTTTGACTTCCTGA.
Vector backbone pX330 (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse centrin 1 genomic DNA
Depositor|Developer Ikawa, Masahito |
 
Other clones in our bank mouse Cetn1 (NCBI Gene 26369) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Mashiko, D., Sci. Rep. 3: 3355 (2013)) The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
Ordering Please visit Information of Request for Distribution.[link] 
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Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
Approval form by depositor [Word]
Remarks ((Additional form)) Approval Form(FormD)
The BIOLOGICAL RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. Please refer the Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose).
Please obtain written approval of the depositor.
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB13533 pX330-Cetn1/sgRNA#1 Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Mashiko, D., Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA. Sci. Rep. 3: 3355 (2013). PMID 24284873.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Genome Editing (English text)
Featured content Genome Editing (Japanese text)

Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


References

Original, user report and related articles

original Mashiko, D., Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA. Sci. Rep. 3: 3355 (2013). PMID 24284873.

2021.03.11

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