Expression plasmid targeting mouse Cetn1 gene in CRISPR/Cas9 system.
Catalog number | RDB13533 |
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Resource name | pX330-Cetn1/sgRNA#1 |
Clone info. | Expression plasmid targeting mouse Cetn1 gene in CRISPR/Cas9 system. MGI ID, 1347086. sgRNA recognition sequence, GGCAACGTTTGACTTCCTGA. |
Vector backbone | pX330 (Plasmid) |
Selectable markers | Amp^r |
Gene/insert name | Mouse centrin 1 genomic DNA |
Depositor|Developer | Ikawa, Masahito | |
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Other clones in our bank | mouse Cetn1 (NCBI Gene 26369) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Mashiko, D., Sci. Rep. 3: 3355 (2013)) The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
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Additional terms and conditions for distribution | Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested. |
Ordering | Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment ![]() ![]() Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word ![]() Approval form by depositor [Word ![]() |
Remarks | ((Additional form)) Approval Form(FormD) The BIOLOGICAL RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. Please refer the Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose). Please obtain written approval of the depositor. |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB13533 | pX330-Cetn1/sgRNA#1 | Under QC test. Please contact us. | DNA solution |
Electronic file
Original reference
original | Mashiko, D., Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA. Sci. Rep. 3: 3355 (2013). PMID 24284873. |
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Further references such as user reports and related articles (go to bottom)
Featured content
Featured content | Genome Editing (English text) |
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Featured content | Genome Editing (Japanese text) |
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
original | Mashiko, D., Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA. Sci. Rep. 3: 3355 (2013). PMID 24284873. |
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2021.03.11
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