Resource data sheet
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pscAAV-CAG-myr-AKT1 (#RDB20988)

Recombinant AAV vector packaging self-complementary myr-AKT1 under the CAG promoter.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Hiraoka, T., Nat. Commun. 16 (1): 5755 (2025). PMID 40595542. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Recombinant AAV vector packaging self-complementary myr-AKT1 (myristoylated Akt1) under the CAG promoter.
Comment The integrity of ITR (positions 21-142) must be confirmed by restriction digestion. For example, when using XhoI and NcoI, the correct fragment length is 422 bp, whereas the loss of the ITR results in a 326 bp fragment.
Vector backbone pscAAV-CAG-GFP (plasmid)
Selectable markers Ampicillin (E. coli)
Gene/insert name mouse Akt1 cDNA; synthetic N-myristoylation signal.
Depositor|Developer Ikawa, Masahito |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Takehiro Hiraoka et al. Nat. Commun., Jul 1;16(1):5755. doi: 10.1038/s41467-025-60610-x. 2025) The RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with The University of Osaka(mta-egr@biken.osaka-u.ac.jp).
Additional terms and conditions:
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit
academic purpose.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Takehiro Hiraoka et al. Nat. Commun., Jul 1;16(1):5755. doi: 10.1038/s41467-025-60610-x. 2025)。利用者が非営利目的の教育・研究以外の目的に用いる場合は、大阪大学(mta-egr@biken.osaka-u.ac.jp)と別途MTAを締結すること。
付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee
RDB20988 pscAAV-CAG-myr-AKT1 DNA solution

How to cite this biological resource

Materials & Methods section:

The pscAAV-CAG-myr-AKT1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20988).

Reference section:

Hiraoka, T., Aikawa, S., Mashiko, D., Nakagawa, T., Shirai, H., Hirota, Y., Kimura, H., Ikawa, M., An ex vivo uterine system captures implantation, embryogenesis, and trophoblast invasion via maternal-embryonic signaling. Nat. Commun. 16 (1): 5755 (2025). PMID 40595542. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Hiraoka, T., An ex vivo uterine system captures implantation, embryogenesis, and trophoblast invasion via maternal-embryonic signaling. Nat. Commun. 16 (1): 5755 (2025). PMID 40595542. [PubMed] [Article] [RRC of NBRP]