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pCMV-Armc12/FLAG/BioID2/HA

Expression vector of mouse Armc12 fused with FLAG, BirA and HA (Armc12-FLAG-BioID2-HA).

Catalog number RDB19467
Resource name pCMV-Armc12/FLAG/BioID2/HA
Clone info. Expression vector of biotin ligase (BirA)-fused mouse armadillo repeat containing 12 (Armc12). HA-tagged BirA was linked posterior to the FLAG-tagged Armc12 (Armc12-FLAG-BioID2-HA). See Fig.S6A of Shimada, K. et al. Proc. Natl. Acad. Sci. USA. 118 (6): e2018355118, 2021.
Comment Expression was confirmed by the depositor with immunoblotting, immunofluorecence.
Vector backbone MCS-BioID2-HA (plasmid)
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Gene/insert name mouse Armc12 cDNA Aquifex aeolicus BioID2 genomic DNA
Depositor|Developer Ikawa, Masahito | Shimada, Keisuke |
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External Database
mouse Armc12 Aquifex aeolicus BioID2

        Reference sequence
          

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Shimada, K. et al., Proc. Natl. Acad. Sci. USA. 118 (6): e2018355118, 2021) RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
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        Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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        提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Shimada, K. et al., Proc. Natl. Acad. Sci. USA. 118 (6): e2018355118, 2021)。利用者が非営利目的の教育・研究以外の目的に用いる場合は、大阪大学と別途MTAを締結すること。
        提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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        提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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        RDB19467 pCMV-Armc12/FLAG/BioID2/HA Under QC test. Please contact us. DNA solution

        check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

        References and tips

        Electronic file

        Original reference

        original Shimada, K., ARMC12 regulates spatiotemporal mitochondrial dynamics during spermiogenesis and is required for male fertility. Proc Natl Acad Sci U S A. 2021 Feb 9;118(6):e2018355118 (2021). PMID 33536340.

        Further references such as user reports and related articles (go to bottom)

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        Sequence information

        check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


        References

        Original, user report and related articles

        original Shimada, K., ARMC12 regulates spatiotemporal mitochondrial dynamics during spermiogenesis and is required for male fertility. Proc Natl Acad Sci U S A. 2021 Feb 9;118(6):e2018355118 (2021). PMID 33536340.

        2022.05.16

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