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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAG_mRsph6a-FLAG

Expression vector of mouse Rsph6a.

Catalog number RDB17453
Resource name pCAG_mRsph6a-FLAG
Alternative name mouse Rsph6a on FLAG tagged pCAG vector
Clone info. Expression vector of mouse radial spoke head 6 homolog A (Rsph6a) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.
Comment Rsph6a was amplified from mouse testis cDNA. Expression was confirmed by the depositor with western blotting.
Vector backbone pCAGGS (plasmid)
Size of vector backbone 4.8 kb
Selectable markers Ampicillin
Gene/insert name mouse Rsph6a cDNA
Depositor|Developer Ikawa, Masahito | Miyata, Haruhiko |
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External Database
mouse Rsph6a

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Abbasi, F et al. J. Cell Sci. 131(19) jcs221648, 2018)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Abbasi, F et al. J. Cell Sci. 131(19) jcs221648, 2018)。
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          RDB17453 pCAG_mRsph6a-FLAG DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence (full) RDB17453zzk02.seq provided by the depositor

          Original reference

          original Abbasi, F., RSPH6A is required for sperm flagellum formation and male fertility in mice. J. Cell Sci. 131 (19). pii: jcs221648 (2018). PMID 30185526.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB17453_A9G9p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pAxCA_F1
          Region: insert 5'
          Sequence file: RDB17453_A9G9a.seq check
          >D06337A5_A9Gg_3_pAxCA_F1_A03_03_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNCC TTCTTCTTTT TCNNNCNGCT CCTGGGCAAC GTGCTGGTTA
             61 TTGTGCTGTC TCNTCATTTT GGCAAATCTA GAGCCGCCAT GGGGGAACCA CCGCCCAATC
            121 CTGACCCCTC TCAAACCCGG AGGGCTTCCC AAGGCTCAGA AAGGGCACGG AGTCAAGAGT
            181 ACTCTCAGCC TCTGTTAACC ATCCCAGAGG ATGGGTTGAA CCGACCACCT CCGCAGCGGG
            241 GCAGTCGGAG CAGTCAGGGC AGTCAGGACC TGCAGGGAAC TGGTCTACCC CACTGGCCAC
            301 AGAGGTCCAG CCTGGTCCCA GATGTTCAAG GTGATGAAGG CACCGAGTAC CACCAGTCCA
            361 TGCCCTTAGG CTACACGCCA GGCTTTCCCA TGGAGTTCTC GCAACAGGGT TACCTGGATG
            421 ATAGCAGGAT GATGGAGCAG TTCCCACAGG GCCAGGACCT CTTAGAACAA CTGGAGTCTA
            481 CCTACCAAGG CTCGGCTTCT GGAATCCTGG GCCAGCTCAA CTTGTATCCA AGAGAGGATG
            541 AGATATTCAG CCAGGACACA CAGCACGGAC CCTACCTGAG AGACGACCCC TCCCTCCACC
            601 TGAGGCCCTC TGACCTGGGC TTCATGCCCA TTGTTGGAGA GGTACCTGAC CCCGAGCCTC
            661 GTGAGCTGGC AATACAGAAC GCCAAGGCCT ACCTGCTGCG GANCAGCATG AGCTGCAACC
            721 TCAGCCTGTA TGAGCACCTG GTGAACCTGC TGANCAAGAT CCTGAACCAG CGCCCTGANG
            781 ANCCCTTGTC CATCCTGGAG TCTCTGAACC GCACCATGCA GTGGGAATGG TTTCACNNCA
            841 AGCTGGACAC GCTGCGGGNN GANCNNNAGA TGCAGCCNAC NTACNANATG GNCGAAAAGC
            901 AAAANNNTCT GTTTATCANG GGTGGNNGAN AAGGNGAANN GGNAATGGNA GANGANGNGN
          961 NNGNNNNNNN NGNNNCNNNN NTNNNNGNNN NNNNNNN
          //
          Primer: pCA-bgter
          Region: FLAG, insert 3'
          Sequence file: RDB17453_A9G9b.seq check
          >D06337A5_A9Gg_3_pCA-bgter_B03_06_ABI24.ab1
              1 NNNNNNNNNN NNNNNNTTTT GNNNGANNNN NNNGNNCTCN GTGGTNTTTG TGAGCCAGGG
             61 CATTGGCCAC ACCAGCCACC ACCTTCTGAT AGGCAGCCTG CACCTGAGGA GTCTCGAGCT
            121 ACTTGTCATC GTCATCCTTG TAGTCGATAT CGAATTCGTC CTCCAGGTCT TCATCCTCCT
            181 CCTCTTCCTC ATCTTCCTCC TCTTCCTCAG CGGCTGCCAG GGCCTGCTCC TGAGCAGCCT
            241 TCAGGGCCTG CTCCTCCTCC ACTGTGGGGT CACTCATCTC CGTGATTTCG GGGCCGCTGG
            301 GGTACTCTTG CTGAATCAGA GCTGGCAGCA TTGGGTTGAA GTTCTCGGGG CTATACTTGT
            361 GACCCCAGCC AATGTATATG TTCTCAAACT TCTTGCCAGT GGCGTAGGCA TAGGCCCCTG
            421 GCCAGAGGTT GGAGCGCACG ATGGCCACCG AGTACTGAGG GCTGAGGCTG CAGGAGAGGC
            481 GGGTGGTCCA GGGCGACAGG TGCATGATTT CTGCATCTTC TGAGAGCGGA GTCAGAAGCG
            541 GAGGACCGAC CTCCTGCTCC ACCTCCTCCA TTGCCTCGTC TGCCTTCTCT TCCTCCTCCC
            601 CTAGCTCTTC TTCCTCCTCT GTTTTCTGCA GTGGGTTCAC CCATGTACAG CGCCCCTGAG
            661 GCAGGATGTG CTGAGTGTGA TGTACCCAGT TGGCCATGGA GTCCACGAGC TCNAACACGG
            721 GGATGCCCTC AAAGTCGGGG TTCTCCTCAA AGGAATCGCG TCCCGCGCCG CCCTCCTCCT
            781 CCTCATCGCC CTCCTCCTCG CCGAANTGGT ANAAGCCCAG CGGGCTGATG TGTGTGGCGG
            841 CGGANANGCG CGCAATCTGC GCGCGGANAN AGTTGGNCTC GTNGCCCGGG AANGGCGGAT
            901 ANCTGATGAN NGGCGNNNNC AGGANNNNAG NGAANAACTT TTTNGANNTT GCNCGCGCNC
          961 NCNNTCTGCG CGGNNNNNNN NNN
          //
          Primer: pUC-CAPbs_up_F
          Region: lac pro, lacO, T3 pro, CMV en, CAG pro
          Sequence file: RDB17453_A9G9c.seq check
          >D06337A5_A9Gg_3_pUC-CAPbs_up_F_C03_09_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NCNNACGCAA TTAATGTGAG TTAGCTCNCT CATTAGGCAC
             61 CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC
            121 AATTTCACAC AGGAAACAGC TATGACCATG ATTACGCCAA GCGCGCAATT AACCCTCACT
            181 AAAGGGAACA AAAGCTGGAG CTCATCGATA CTCGACATTG ATTATTGACT AGTTATTAAT
            241 AGTAATCAAT TACGGGGTCA TTAGTTCATA GCCCATATAT GGAGTTCCGC GTTACATAAC
            301 TTACGGTAAA TGGCCCGCCT GGCTGACCGC CCAACGACCC CCGCCCATTG ACGTCAATAA
            361 TGACGTATGT TCCCATAGTA ACGCCAATAG GGACTTTCCA TTGACGTCAA TGGGTGGAGT
            421 ATTTACGGTA AACTGCCCAC TTGGCAGTAC ATCAAGTGTA TCATATGCCA AGTACGCCCC
            481 CTATTGACGT CAATGACGGT AAATGGCCCG CCTGGCATTA TGCCCAGTAC ATGACCTTAT
            541 GGGACTTTCC TACTTGGCAG TACATCTACG TATTAGTCAT CGCTATTACC ATGGTCGAGG
            601 TGAGCCCCAC GTTCTGCTTC ACTCTCCCCA TCTCCCCCCC CTCCCCACCC CCAATTTTGT
            661 ATTTATTTAT TTTTTAATTA TTTTGTGCAG CGATGGGGGC NGGGGGGGGG GGGGGNNNNN
          721 NNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Abbasi, F., RSPH6A is required for sperm flagellum formation and male fertility in mice. J. Cell Sci. 131 (19). pii: jcs221648 (2018). PMID 30185526.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl