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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAG_mRsph6a-FLAG

Expression vector of mouse Rsph6a.

Catalog number RDB17453
Resource name pCAG_mRsph6a-FLAG
Alternative name mouse Rsph6a on FLAG tagged pCAG vector
Clone info. Expression vector of mouse radial spoke head 6 homolog A (Rsph6a) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.
Rsph6a was amplified from mouse testis cDNA. Expression was confirmed by the depositor with western blotting.
Vector backbone pCAGGS (plasmid)
Size of vector backbone 4.8 kb
Selectable markers Ampicillin
Gene/insert name mouse Rsph6a cDNA
Depositor|Developer Ikawa, Masahito | Miyata, Haruhiko |
 
Other clones in our bank mouse Rsph6a (NCBI Gene 83434) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Abbasi, F et al. J. Cell Sci. 131(19) jcs221648, 2018)
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RDB17453 pCAG_mRsph6a-FLAG DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Sequence (full) RDB17453zzk02.seq provided by the depositor

Original reference

original Abbasi, F., RSPH6A is required for sperm flagellum formation and male fertility in mice. J. Cell Sci. 131 (19). pii: jcs221648 (2018). PMID 30185526.

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17453_A9G9p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1
Region: insert 5'
Sequence file: RDB17453_A9G9a.seq check
>D06337A5_A9Gg_3_pAxCA_F1_A03_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNCC TTCTTCTTTT TCNNNCNGCT CCTGGGCAAC GTGCTGGTTA
   61 TTGTGCTGTC TCNTCATTTT GGCAAATCTA GAGCCGCCAT GGGGGAACCA CCGCCCAATC
  121 CTGACCCCTC TCAAACCCGG AGGGCTTCCC AAGGCTCAGA AAGGGCACGG AGTCAAGAGT
  181 ACTCTCAGCC TCTGTTAACC ATCCCAGAGG ATGGGTTGAA CCGACCACCT CCGCAGCGGG
  241 GCAGTCGGAG CAGTCAGGGC AGTCAGGACC TGCAGGGAAC TGGTCTACCC CACTGGCCAC
  301 AGAGGTCCAG CCTGGTCCCA GATGTTCAAG GTGATGAAGG CACCGAGTAC CACCAGTCCA
  361 TGCCCTTAGG CTACACGCCA GGCTTTCCCA TGGAGTTCTC GCAACAGGGT TACCTGGATG
  421 ATAGCAGGAT GATGGAGCAG TTCCCACAGG GCCAGGACCT CTTAGAACAA CTGGAGTCTA
  481 CCTACCAAGG CTCGGCTTCT GGAATCCTGG GCCAGCTCAA CTTGTATCCA AGAGAGGATG
  541 AGATATTCAG CCAGGACACA CAGCACGGAC CCTACCTGAG AGACGACCCC TCCCTCCACC
  601 TGAGGCCCTC TGACCTGGGC TTCATGCCCA TTGTTGGAGA GGTACCTGAC CCCGAGCCTC
  661 GTGAGCTGGC AATACAGAAC GCCAAGGCCT ACCTGCTGCG GANCAGCATG AGCTGCAACC
  721 TCAGCCTGTA TGAGCACCTG GTGAACCTGC TGANCAAGAT CCTGAACCAG CGCCCTGANG
  781 ANCCCTTGTC CATCCTGGAG TCTCTGAACC GCACCATGCA GTGGGAATGG TTTCACNNCA
  841 AGCTGGACAC GCTGCGGGNN GANCNNNAGA TGCAGCCNAC NTACNANATG GNCGAAAAGC
  901 AAAANNNTCT GTTTATCANG GGTGGNNGAN AAGGNGAANN GGNAATGGNA GANGANGNGN
961 NNGNNNNNNN NGNNNCNNNN NTNNNNGNNN NNNNNNN
//
Primer: pCA-bgter
Region: FLAG, insert 3'
Sequence file: RDB17453_A9G9b.seq check
>D06337A5_A9Gg_3_pCA-bgter_B03_06_ABI24.ab1
    1 NNNNNNNNNN NNNNNNTTTT GNNNGANNNN NNNGNNCTCN GTGGTNTTTG TGAGCCAGGG
   61 CATTGGCCAC ACCAGCCACC ACCTTCTGAT AGGCAGCCTG CACCTGAGGA GTCTCGAGCT
  121 ACTTGTCATC GTCATCCTTG TAGTCGATAT CGAATTCGTC CTCCAGGTCT TCATCCTCCT
  181 CCTCTTCCTC ATCTTCCTCC TCTTCCTCAG CGGCTGCCAG GGCCTGCTCC TGAGCAGCCT
  241 TCAGGGCCTG CTCCTCCTCC ACTGTGGGGT CACTCATCTC CGTGATTTCG GGGCCGCTGG
  301 GGTACTCTTG CTGAATCAGA GCTGGCAGCA TTGGGTTGAA GTTCTCGGGG CTATACTTGT
  361 GACCCCAGCC AATGTATATG TTCTCAAACT TCTTGCCAGT GGCGTAGGCA TAGGCCCCTG
  421 GCCAGAGGTT GGAGCGCACG ATGGCCACCG AGTACTGAGG GCTGAGGCTG CAGGAGAGGC
  481 GGGTGGTCCA GGGCGACAGG TGCATGATTT CTGCATCTTC TGAGAGCGGA GTCAGAAGCG
  541 GAGGACCGAC CTCCTGCTCC ACCTCCTCCA TTGCCTCGTC TGCCTTCTCT TCCTCCTCCC
  601 CTAGCTCTTC TTCCTCCTCT GTTTTCTGCA GTGGGTTCAC CCATGTACAG CGCCCCTGAG
  661 GCAGGATGTG CTGAGTGTGA TGTACCCAGT TGGCCATGGA GTCCACGAGC TCNAACACGG
  721 GGATGCCCTC AAAGTCGGGG TTCTCCTCAA AGGAATCGCG TCCCGCGCCG CCCTCCTCCT
  781 CCTCATCGCC CTCCTCCTCG CCGAANTGGT ANAAGCCCAG CGGGCTGATG TGTGTGGCGG
  841 CGGANANGCG CGCAATCTGC GCGCGGANAN AGTTGGNCTC GTNGCCCGGG AANGGCGGAT
  901 ANCTGATGAN NGGCGNNNNC AGGANNNNAG NGAANAACTT TTTNGANNTT GCNCGCGCNC
961 NCNNTCTGCG CGGNNNNNNN NNN
//
Primer: pUC-CAPbs_up_F
Region: lac pro, lacO, T3 pro, CMV en, CAG pro
Sequence file: RDB17453_A9G9c.seq check
>D06337A5_A9Gg_3_pUC-CAPbs_up_F_C03_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NCNNACGCAA TTAATGTGAG TTAGCTCNCT CATTAGGCAC
   61 CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC
  121 AATTTCACAC AGGAAACAGC TATGACCATG ATTACGCCAA GCGCGCAATT AACCCTCACT
  181 AAAGGGAACA AAAGCTGGAG CTCATCGATA CTCGACATTG ATTATTGACT AGTTATTAAT
  241 AGTAATCAAT TACGGGGTCA TTAGTTCATA GCCCATATAT GGAGTTCCGC GTTACATAAC
  301 TTACGGTAAA TGGCCCGCCT GGCTGACCGC CCAACGACCC CCGCCCATTG ACGTCAATAA
  361 TGACGTATGT TCCCATAGTA ACGCCAATAG GGACTTTCCA TTGACGTCAA TGGGTGGAGT
  421 ATTTACGGTA AACTGCCCAC TTGGCAGTAC ATCAAGTGTA TCATATGCCA AGTACGCCCC
  481 CTATTGACGT CAATGACGGT AAATGGCCCG CCTGGCATTA TGCCCAGTAC ATGACCTTAT
  541 GGGACTTTCC TACTTGGCAG TACATCTACG TATTAGTCAT CGCTATTACC ATGGTCGAGG
  601 TGAGCCCCAC GTTCTGCTTC ACTCTCCCCA TCTCCCCCCC CTCCCCACCC CCAATTTTGT
  661 ATTTATTTAT TTTTTAATTA TTTTGTGCAG CGATGGGGGC NGGGGGGGGG GGGGGNNNNN
721 NNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Abbasi, F., RSPH6A is required for sperm flagellum formation and male fertility in mice. J. Cell Sci. 131 (19). pii: jcs221648 (2018). PMID 30185526.

2021.08.20

GNP_filter3_RDBDEP_html_210618.pl