RDB19439 - pClgn-Lrrc23-FLAG

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pClgn-Lrrc23-FLAG

Expression vector of mouse Lrrc23 tagged with FLAG at C-terminus, driven by Calmergin promoter.

Catalog number	RDB19439
Resource name	pClgn-Lrrc23-FLAG
Clone info.	Expression vector of mouse leucine rich repeat containing 23 (Lrrc23) tagged with FLAG at C-terminus, Calmergin (Clgn) promoter, poly (A) signal. See Fig.8A of Zhang, X. et al. J. Cell Sci. jcs.259381, 2021 (134 (20)).
Comment	Expression was confirmed by the depositor with immunoblotting
Vector backbone	pClgn (plasmid)
Selectable markers	Ampicillin
Gene/insert name	mouse Lrrc23 cDNA
Depositor\|Developer	Ikawa, Masahito \| Lu, Yonggang \|

Other clones in our bank

mouse Lrrc23

External Database
mouse Lrrc23

Reference sequence

Sequence (full)	RDB19439hts01.seq assembled from experimentally sequenced data
Test sheet	RDB19439_B1L2p1-1.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Zhang, X. et al. J. Cell Sci. 134(20): jcs.259381, 2021). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.

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Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Zhang, X. et al. J. Cell Sci. 134(20): jcs.259381, 2021). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
Ordering	Order form [Credit Card Payment] [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Zhang, X. et al., J. Cell Sci. 134(20): jcs.259381, 2021)。利用者が非営利目的の教育・研究以外の目的に用いる場合は、大阪大学と別途MTAを締結すること。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB19439	pClgn-Lrrc23-FLAG	DNA solution

How to cite this biological resource

Materials & Methods section:

The pClgn-Lrrc23-FLAG was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19439).

Reference section:

Zhang, X., Sun, J., Lu, Y., Zhang, J,. Shimada, K., Noda, T., Zhao, S., Koyano, T., Matsuyama, M., Zhou, S., Wu, J., Ikawa, M., Liu, M., LRRC23 is a conserved component of the radial spoke that is necessary for sperm motility and male fertility in mice. J. Cell Sci. 134 (20): jcs259381 (2021). PMID 34585727. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

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References

Original, user report and related articles

original	Zhang, X., LRRC23 is a conserved component of the radial spoke that is necessary for sperm motility and male fertility in mice. J. Cell Sci. 134 (20): jcs259381 (2021). PMID 34585727. [link to RRC of NBRP]

2023.12.04

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