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pClgn-Lrrc23-FLAG (#RDB19439)

Expression vector of mouse Lrrc23 tagged with FLAG at C-terminus, driven by Calmergin promoter.


Drawn by SnapGene® software
Sequence (full) RDB19439hts01.seq
Publication Zhang, X., J. Cell Sci. 134 (20): jcs259381 (2021). [link to RRC of NBRP]
Test sheet RDB19439_B1L2p1-1.pdf 
Assembled from experimentally sequenced data.
 
Clone info. Expression vector of mouse leucine rich repeat containing 23 (Lrrc23) tagged with FLAG at C-terminus, Calmergin (Clgn) promoter, poly (A) signal. See Fig.8A of Zhang, X. et al. J. Cell Sci. jcs.259381, 2021 (134 (20)).
Comment Expression was confirmed by the depositor with immunoblotting
Vector backbone pClgn (plasmid)
Selectable markers Ampicillin
Gene/insert name mouse Lrrc23 cDNA
Depositor|Developer Ikawa, Masahito | Lu, Yonggang |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Zhang, X. et al. J. Cell Sci. 134(20): jcs.259381, 2021). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Zhang, X. et al., J. Cell Sci. 134(20): jcs.259381, 2021)。利用者が非営利目的の教育・研究以外の目的に用いる場合は、大阪大学と別途MTAを締結すること。

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RDB19439 pClgn-Lrrc23-FLAG DNA solution

How to cite this biological resource

Materials & Methods section:

The pClgn-Lrrc23-FLAG was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19439).

Reference section:

Zhang, X., Sun, J., Lu, Y., Zhang, J,. Shimada, K., Noda, T., Zhao, S., Koyano, T., Matsuyama, M., Zhou, S., Wu, J., Ikawa, M., Liu, M., LRRC23 is a conserved component of the radial spoke that is necessary for sperm motility and male fertility in mice. J. Cell Sci. 134 (20): jcs259381 (2021). PMID 34585727. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Zhang, X., LRRC23 is a conserved component of the radial spoke that is necessary for sperm motility and male fertility in mice. J. Cell Sci. 134 (20): jcs259381 (2021). PMID 34585727. [link to RRC of NBRP]