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pCAG-mPpp3cc-FLAG-6XHIS

Expression vector of mouse Ppp3cc with FLAG- and 6xHIS-tags.

Catalog number RDB19406
Resource name pCAG-mPpp3cc-FLAG-6XHIS
Clone info. Expression vector of mouse protein phosphatase 3, catalytic subunit, gamma isoform (Ppp3cc) tagged with FLAG and 6xHIS at C-terminus, CAG promoter, rabbit globin poly(A) signal.
Comment Expression was confirmed by the depositor with western blotting.
Vector backbone pCAGGS (plasmid)
Size of vector backbone 5.2 kb
Selectable markers Ampicillin
Gene/insert name mouse Ppp3cc cDNA
Depositor|Developer Ikawa, Masahito |
 
Sequence (full) RDB19406hts01.seqcheck assembled from experimentally sequenced data
Test sheet RDB19406_B1Kbp1-1.pdf 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Miyata, H. et al., Science, 350 (6259): 442-445, 2015). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
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MTA, for use for not-for-profit academic purpose [Word]
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提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Miyata, H. et al., Science, 350 (6259): 442-445, 2015)。利用者が非営利目的の教育・研究以外の目的に用いる場合は、大阪大学と別途MTAを締結すること。
付加的提供条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
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提供同意書 (MTA, 非営利学術目的用)[Word]
提供同意書 (MTA, 営利目的用)[Word]
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19406 pCAG-mPpp3cc-FLAG-6XHIS DNA solution

How to cite this biological resource

Materials & Methods section:

The pCAG-mPpp3cc-FLAG-6XHIS was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19406).

Reference section:

Miyata, H., Satouh, Y., Mashiko, D., Muto, M., Nozawa, K., Shiba, K., Fujihara, Y., Isotani, A., Inaba, K., Ikawa, M., Sperm calcineurin inhibition prevents mouse fertility with implications for male contraceptive. Science 350 (6259): 442-445 (2015). PMID 26429887. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Miyata, H., Sperm calcineurin inhibition prevents mouse fertility with implications for male contraceptive. Science 350 (6259): 442-445 (2015). PMID 26429887. [link to RRC of NBRP]