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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAG_mRsph9-PA

Expression vector of mouse Rsph9.

Catalog number RDB17451
Resource name pCAG_mRsph9-PA
Alternative name mouse Rsph9 on PA tagged pCAG vector
Clone info. Expression vector of mouse radial spoke head 9 homolog (Rsph9) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal.
Comment Rsph9 was amplified from mouse testis cDNA. The PA tag is a dodecapeptide (GVAMPGAEDDVV) that is recognized by this high-affinity antibody. Expression was confirmed by the depositor with western blotting.
Vector backbone pCAGGS (plasmid)
Size of vector backbone 4.8 kb
Selectable markers Ampicillin
Gene/insert name mouse Rsph9 cDNA
Depositor|Developer Ikawa, Masahito | Miyata, Haruhiko |
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External Database
mouse Rsph9

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Abbasi, F et al. J. Cell Sci. 131(19) jcs221648, 2018)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Abbasi, F et al. J. Cell Sci. 131(19) jcs221648, 2018)。
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          RDB17451 pCAG_mRsph9-PA DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence (full) RDB17451zzk02.seq provided by the depositor

          Original reference

          original Abbasi, F., RSPH6A is required for sperm flagellum formation and male fertility in mice. J. Cell Sci. 131 (19). pii: jcs221648 (2018). PMID 30185526.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB17451_A9G9p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pAxCA_F1
          Region: insert 5'
          Sequence file: RDB17451_A9G9a.seq check
          >D06337A3_A9Gg_1_pAxCA_F1_A02_02_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNGCC TTCTTCTTTT NCCTNCNGCT CCTGGGCAAC GTGCTGGTTA
             61 TTGTGCTGTC NNNNNNNNTT GNCAAATCTA GAGCCGCCAT GGACGCCGAC AGCCTCTTGT
            121 TGTCTCTGGA GTTGGCGTCT GGCAGTGGGC AGGGACTCAG CCCTGACCGT CGGGCCTCAC
            181 TGCTCACGTC CCTTATGCTG GTGAAGCGCG ATTACCGCTT CGCGAGGGTC CTTTTCTGGG
            241 GCCGCATCCT TGGCCTTGTG GCGGATTACT ACATCGCACA GGGCTTGAGT GAGGACCAGC
            301 TCGCACCACG CAAGACGCTC TACAGCCTGA ACTGCACAGA GTGGAGTCTC TTGCCCCCTG
            361 CCACAGAGGA GATGGCCATG CAGATATCTG TGGTGAGTGG CCGTTTCATG GGCGACCCTT
            421 CACACGAGTA TGAACACACA GAGTTGCAGA AGGTTAACGA AGGAGAGAAG GTCTTTGATG
            481 AAGAAGTGGT GGTCCAGATC AAGGAAGAGA CTCGCTTGGT GTCCATCATT GACCAGATTG
            541 ACAAGGCTGT AGCTATCATC CCTCGAGGTG CCCTCTTCAA GACCCCTTTT GGAGTCACCC
            601 ATGTCAATCG GACCTTTGAA GGCCTGCCCC TGTCCGAGGT CAGGAAGCTT AGCTCGTACT
            661 TCCACTTCAG GGAGGCTATT GATCTGAAGA ACAAGACCTT GCTGGAGAAG TCGGACTTGG
            721 AGCCCTCGCT GGATTTCCTG GACTCCCTGG AATATGACAT CCCCAGANGG TCTTGGANCA
            781 TCCAGATGGA AAGGGGCAAC GCACTGGTGG TGCTGCGCAN CCTGCTCTGG CCAGGCCTCA
            841 CCTTCTACCA CGCTCCTCGC ACCNAGAACT ATGGCTACNT CTACGTANGC ACAGGANANA
            901 AGAACATGGA CNNGCCNNNC ANGCTGGAAN TCGATATCNA GCTNGNCGAN NNNGCCNNCN
          961 ANGNNANCNG NNNTNNNNTN NCNNNNNNNN NN
          //
          Primer: pCA-bgter
          Region: PA tag, insert 3'
          Sequence file: RDB17451_A9G9b.seq check
          >D06337A3_A9Gg_1_pCA-bgter_B02_05_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNTT TTTGNNNGAN GNNNNNNNNC TCNGTGGTAT TTGTGAGCCA
             61 GGGCATTGGC CACACCAGCC ACCACCTTCT GATAGGCAGC CTGCACCTGA GGAGTCTCGA
            121 TTTACACCAC ATCATCTTCG GCACCTGGCA TGGCAACGCC GCTAGCCTCG AGGGCCGCGT
            181 CGACAAGCTT GATATCGAAT TCCAGCATGA AGGGCAAGTC CATGTTCTTC TCTCCTGTGC
            241 CTACGTAGAT GTAGCCATAG TTCTTGGTGC GAGGAGCGTG GTAGAAGGTG AGGCCTGGCC
            301 AGAGCAGGCT GCGCAGCACC ACCAGTGCGT TGCCCCTTTC CATCTGGATG CTCCAAGACC
            361 CTCTGGGGAT GTCATATTCC AGGGAGTCCA GGAAATCCAG CGAGGGCTCC AAGTCCGACT
            421 TCTCCAGCAA GGTCTTGTTC TTCAGATCAA TAGCCTCCCT GAAGTGGAAG TACGAGCTAA
            481 GCTTCCTGAC CTCGGACAGG GGCAGGCCTT CAAAGGTCCG ATTGACATGG GTGACTCCAA
            541 AAGGGGTCTT GAAGAGGGCA CCTCGAGGGA TGATAGCTAC AGCCTTGTCA ATCTGGTCAA
            601 TGATGGACAC CAAGCGAGTC TCTTCCTTGA TCTGGACCAC CACTTCTTCA TCAAAGACCT
            661 TCTCTCCTTC GTTAACCTTC TGCAACTCTG TGTGTTCATA CTCGTGTGAA GGGTCGCCCA
            721 TGAAACGGCC ACTCACCACA GATATCTGCA TGGCCATCTC CTCTGTGNCA GGGGGCAAGA
            781 GACTCCACTC TGTGCAGTTC ANGCTGTAGA GCGTCTTGCG TGGTGCGAGC TGGTCCTCAC
            841 TCAAGCCCNG TGCGATGTAG TAATCCGCCA CAAGGCCNAG GATGCGGCCC CAGAAAAGGA
            901 NNNTCGCGAA NCNGTAATCG CGCTTNNCCA GCATANNGGA NGTGANCANN GANGCCNNAC
          961 GGNCNNNNCT NANNNNNNGN NNNNNNNNN
          //
          Primer: pUC-CAPbs_up_F
          Region: lac pro, lacO, T3 pro, CMV en, CAG pro
          Sequence file: RDB17451_A9G9c.seq check
          >D06337A3_A9Gg_1_pUC-CAPbs_up_F_C02_08_ABI24.ab1
              1 NNNNNNNNNN NNNGNNNNNN NNNCGCANGC NNNAATGTGA GTTAGCTCNC TNNTTAGGCA
             61 CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA
            121 CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGCCA AGCGCGCAAT TAACCCTCAC
            181 TAAAGGGAAC AAAAGCTGGA GCTCATCGAT ACTCGACATT GATTATTGAC TAGTTATTAA
            241 TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA
            301 CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA
            361 ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG
            421 TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC
            481 CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA
            541 TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTCGAG
            601 GTGAGCCCCA CGTTCTGCTT CACTCTCCCC ATCTCCCCCC CCTCCCCACC CCCAATTTTG
            661 TATTTATTTA TTTTTTAATT ATTTTGTGCA GCGATGGGGG CNNNGGGGGG GGNNNNNNNN
          721 GNNGNGNNNN NNGNNNNNNN NNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Abbasi, F., RSPH6A is required for sperm flagellum formation and male fertility in mice. J. Cell Sci. 131 (19). pii: jcs221648 (2018). PMID 30185526.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl