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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAG_mRsph9-PA (#RDB17451)

Expression vector of mouse Rsph9.

Alternative name mouse Rsph9 on PA tagged pCAG vector
Clone info. Expression vector of mouse radial spoke head 9 homolog (Rsph9) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal.
Comment Rsph9 was amplified from mouse testis cDNA. The PA tag is a dodecapeptide (GVAMPGAEDDVV) that is recognized by this high-affinity antibody. Expression was confirmed by the depositor with western blotting.
Vector backbone pCAGGS (plasmid)
Size of vector backbone 4.8 kb
Selectable markers Ampicillin
Gene/insert name mouse Rsph9 cDNA
Depositor|Developer Ikawa, Masahito | Miyata, haruhiko |
 
Sequence (full) RDB17451zzk02.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Abbasi, F et al. J. Cell Sci. 131(19) jcs221648, 2018)
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Abbasi, F et al. J. Cell Sci. 131(19) jcs221648, 2018)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17451 pCAG_mRsph9-PA DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCAG_mRsph9-PA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17451).

Reference section:

Abbasi, F., Miyata, H., Shimada, K., Morohoshi, A., Nozawa, K., Matsumura, T., Xu, Z., Pratiwi, P., Ikawa, M., RSPH6A is required for sperm flagellum formation and male fertility in mice. J. Cell Sci. 131 (19). pii: jcs221648 (2018). PMID 30185526. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17451_A9G9p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 (Pr0164)
Region: insert 5'
Sequence file: RDB17451_A9G9a.seq check
>D06337A3_A9Gg_1_pAxCA_F1_A02_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGCC TTCTTCTTTT NCCTNCNGCT CCTGGGCAAC GTGCTGGTTA
   61 TTGTGCTGTC NNNNNNNNTT GNCAAATCTA GAGCCGCCAT GGACGCCGAC AGCCTCTTGT
  121 TGTCTCTGGA GTTGGCGTCT GGCAGTGGGC AGGGACTCAG CCCTGACCGT CGGGCCTCAC
  181 TGCTCACGTC CCTTATGCTG GTGAAGCGCG ATTACCGCTT CGCGAGGGTC CTTTTCTGGG
  241 GCCGCATCCT TGGCCTTGTG GCGGATTACT ACATCGCACA GGGCTTGAGT GAGGACCAGC
  301 TCGCACCACG CAAGACGCTC TACAGCCTGA ACTGCACAGA GTGGAGTCTC TTGCCCCCTG
  361 CCACAGAGGA GATGGCCATG CAGATATCTG TGGTGAGTGG CCGTTTCATG GGCGACCCTT
  421 CACACGAGTA TGAACACACA GAGTTGCAGA AGGTTAACGA AGGAGAGAAG GTCTTTGATG
  481 AAGAAGTGGT GGTCCAGATC AAGGAAGAGA CTCGCTTGGT GTCCATCATT GACCAGATTG
  541 ACAAGGCTGT AGCTATCATC CCTCGAGGTG CCCTCTTCAA GACCCCTTTT GGAGTCACCC
  601 ATGTCAATCG GACCTTTGAA GGCCTGCCCC TGTCCGAGGT CAGGAAGCTT AGCTCGTACT
  661 TCCACTTCAG GGAGGCTATT GATCTGAAGA ACAAGACCTT GCTGGAGAAG TCGGACTTGG
  721 AGCCCTCGCT GGATTTCCTG GACTCCCTGG AATATGACAT CCCCAGANGG TCTTGGANCA
  781 TCCAGATGGA AAGGGGCAAC GCACTGGTGG TGCTGCGCAN CCTGCTCTGG CCAGGCCTCA
  841 CCTTCTACCA CGCTCCTCGC ACCNAGAACT ATGGCTACNT CTACGTANGC ACAGGANANA
  901 AGAACATGGA CNNGCCNNNC ANGCTGGAAN TCGATATCNA GCTNGNCGAN NNNGCCNNCN
961 ANGNNANCNG NNNTNNNNTN NCNNNNNNNN NN
//
Primer: pCA-bgter (Pr0066)
Region: PA tag, insert 3'
Sequence file: RDB17451_A9G9b.seq check
>D06337A3_A9Gg_1_pCA-bgter_B02_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNTT TTTGNNNGAN GNNNNNNNNC TCNGTGGTAT TTGTGAGCCA
   61 GGGCATTGGC CACACCAGCC ACCACCTTCT GATAGGCAGC CTGCACCTGA GGAGTCTCGA
  121 TTTACACCAC ATCATCTTCG GCACCTGGCA TGGCAACGCC GCTAGCCTCG AGGGCCGCGT
  181 CGACAAGCTT GATATCGAAT TCCAGCATGA AGGGCAAGTC CATGTTCTTC TCTCCTGTGC
  241 CTACGTAGAT GTAGCCATAG TTCTTGGTGC GAGGAGCGTG GTAGAAGGTG AGGCCTGGCC
  301 AGAGCAGGCT GCGCAGCACC ACCAGTGCGT TGCCCCTTTC CATCTGGATG CTCCAAGACC
  361 CTCTGGGGAT GTCATATTCC AGGGAGTCCA GGAAATCCAG CGAGGGCTCC AAGTCCGACT
  421 TCTCCAGCAA GGTCTTGTTC TTCAGATCAA TAGCCTCCCT GAAGTGGAAG TACGAGCTAA
  481 GCTTCCTGAC CTCGGACAGG GGCAGGCCTT CAAAGGTCCG ATTGACATGG GTGACTCCAA
  541 AAGGGGTCTT GAAGAGGGCA CCTCGAGGGA TGATAGCTAC AGCCTTGTCA ATCTGGTCAA
  601 TGATGGACAC CAAGCGAGTC TCTTCCTTGA TCTGGACCAC CACTTCTTCA TCAAAGACCT
  661 TCTCTCCTTC GTTAACCTTC TGCAACTCTG TGTGTTCATA CTCGTGTGAA GGGTCGCCCA
  721 TGAAACGGCC ACTCACCACA GATATCTGCA TGGCCATCTC CTCTGTGNCA GGGGGCAAGA
  781 GACTCCACTC TGTGCAGTTC ANGCTGTAGA GCGTCTTGCG TGGTGCGAGC TGGTCCTCAC
  841 TCAAGCCCNG TGCGATGTAG TAATCCGCCA CAAGGCCNAG GATGCGGCCC CAGAAAAGGA
  901 NNNTCGCGAA NCNGTAATCG CGCTTNNCCA GCATANNGGA NGTGANCANN GANGCCNNAC
961 GGNCNNNNCT NANNNNNNGN NNNNNNNNN
//
Primer: pUC-CAPbs_up_F (Pr0536)
Region: lac pro, lacO, T3 pro, CMV en, CAG pro
Sequence file: RDB17451_A9G9c.seq check
>D06337A3_A9Gg_1_pUC-CAPbs_up_F_C02_08_ABI24.ab1
    1 NNNNNNNNNN NNNGNNNNNN NNNCGCANGC NNNAATGTGA GTTAGCTCNC TNNTTAGGCA
   61 CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA
  121 CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGCCA AGCGCGCAAT TAACCCTCAC
  181 TAAAGGGAAC AAAAGCTGGA GCTCATCGAT ACTCGACATT GATTATTGAC TAGTTATTAA
  241 TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA
  301 CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA
  361 ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG
  421 TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC
  481 CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA
  541 TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTCGAG
  601 GTGAGCCCCA CGTTCTGCTT CACTCTCCCC ATCTCCCCCC CCTCCCCACC CCCAATTTTG
  661 TATTTATTTA TTTTTTAATT ATTTTGTGCA GCGATGGGGG CNNNGGGGGG GGNNNNNNNN
721 GNNGNGNNNN NNGNNNNNNN NNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Abbasi, F., RSPH6A is required for sperm flagellum formation and male fertility in mice. J. Cell Sci. 131 (19). pii: jcs221648 (2018). PMID 30185526. [link to RRC of NBRP]