Resource data sheet
Prev.
Please review the HTS results indicated by check icon below as well as clone information before placing your order.

pCAG-hSPATA33-8xHIS-1D4

Expression vector of human SPATA33 with 8xHIS- and 1D4-tags.

Catalog number RDB19402
Resource name pCAG-hSPATA33-8xHIS-1D4
Clone info. Expression vector of human spermatogenesis associated 33 (SPATA33) tagged with 8xHIS and 1D4 at C-terminus, CAG promoter, rabbit globin poly(A) signal.
Comment Expression was confirmed by the depositor with western blotting.
Vector backbone pCAGGS (plasmid)
Size of vector backbone 5.2 kb
Selectable markers Ampicillin
Gene/insert name human SPATA33 cDNA
Depositor|Developer Ikawa, Masahito |
Other clones in our bank

External Database
human SPATA33

          Reference sequence
            
           
          Sequence (full) RDB19402hts01.seqcheck assembled from experimentally sequenced data
          Test sheet RDB19402_B1Kbp1-1.pdf 

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Miyata, H. et al., Proc. Natl. Acad. Sci. USA. 118 (35): e2106673118, 2021). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
          Additional terms and conditions:
          Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
          Ordering
          Order form [Credit Card Payment]  [Bank Transfer Payment]
          MTA, for use for not-for-profit academic purpose [Word]
          MTA, for use for for-profit purpose [Word]
          Please visit Information of Request for Distribution.[link] 
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Miyata, H. et al., Proc. Natl. Acad. Sci. USA. 118 (35): e2106673118, 2021)。利用者が非営利目的の教育・研究以外の目的に用いる場合は、大阪大学と別途MTAを締結すること。
          付加的提供条件:
          CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
          提供依頼
          提供依頼書 [Word]
          提供同意書 (MTA, 非営利学術目的用)[Word]
          提供同意書 (MTA, 営利目的用)[Word]
          手続きの概要は、「提供申込みについて[link]」をご覧ください。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB19402 pCAG-hSPATA33-8xHIS-1D4 DNA solution


          How to cite this biological resource

          Materials & Methods section:

          The pCAG-hSPATA33-8xHIS-1D4 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19402).

          Reference section:

          Miyata, H., Oura, S., Morohoshi, A., Shimada, K., Mashiko, D., Oyama, Y., Kaneda, Y., Matsumura, T., Abbasi, F., Ikawa, M., SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice. Proc. Natl. Acad. Sci. U.S.A. 118 (35): e2106673118 (2021). PMID 34446558. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


          Tips

          Featured content


          References

          Original, user report and related articles

          original Miyata, H., SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice. Proc. Natl. Acad. Sci. U.S.A. 118 (35): e2106673118 (2021). PMID 34446558. [link to RRC of NBRP]

          2023.12.04

          GNP_filter3_RDBDEP_html_231027.pl