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pCAG-mPpp3r2

Expression vector of mouse Ppp3r2.

Catalog number RDB19407
Resource name pCAG-mPpp3r2
Clone info. Expression vector of mouse protein phosphatase 3, regulatory subunit B, beta isoform (Ppp3r2) at C-terminus, CAG promoter, rabbit globin poly(A) signal.
Comment Expression was confirmed by the depositor with western blotting.
Vector backbone pCAGGS (plasmid)
Size of vector backbone 5.2 kb
Selectable markers Ampicillin
Gene/insert name mouse Ppp3r2 cDNA
Depositor|Developer Ikawa, Masahito |
Other clones in our bank
External Database
mouse Ppp3r2
          Reference sequence
            
           
          Sequence (full) RDB19407hts01.seqcheck assembled from experimentally sequenced data
          Test sheet RDB19407_B1Kbp1-1.pdf 

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Miyata, H. et al., Science, 350 (6259): 442-445, 2015). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
          Additional terms and conditions:
          Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
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          MTA, for use for not-for-profit academic purpose [Word]
          MTA, for use for for-profit purpose [Word]
          Please visit Information of Request for Distribution.[link] 
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Miyata, H. et al., Science, 350 (6259): 442-445, 2015)。利用者が非営利目的の教育・研究以外の目的に用いる場合は、大阪大学と別途MTAを締結すること。
          付加的提供条件：
          CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
          提供依頼
          提供依頼書 [Word]
          提供同意書 (MTA, 非営利学術目的用)[Word]
          提供同意書 (MTA, 営利目的用)[Word]
          手続きの概要は、「提供申込みについて[link]」をご覧ください。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB19407 pCAG-mPpp3r2 DNA solution

          How to cite this biological resource

          Materials & Methods section:

          The pCAG-mPpp3r2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19407).

          Reference section:

          Miyata, H., Satouh, Y., Mashiko, D., Muto, M., Nozawa, K., Shiba, K., Fujihara, Y., Isotani, A., Inaba, K., Ikawa, M., Sperm calcineurin inhibition prevents mouse fertility with implications for male contraceptive. Science 350 (6259): 442-445 (2015). PMID 26429887. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

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          References

          Original, user report and related articles

          original Miyata, H., Sperm calcineurin inhibition prevents mouse fertility with implications for male contraceptive. Science 350 (6259): 442-445 (2015). PMID 26429887. [link to RRC of NBRP]

          2023.12.04

          GNP_filter3_RDBDEP_html_231027.pl