Resource data sheet
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pscAAV-CAG-Cre (#RDB20979)

Recombinant AAV vector packaging self-complementary Cre under the CAG promoter.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Nakagawa, T., Exp. Anim. 74 (3): 310-318 (2025). PMID 39805595. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Recombinant AAV vector packaging self-complementary Cre under the CAG promoter.
Comment The integrity of ITR (positions 21-142) must be confirmed by restriction digestion. For example, when using XhoI and NcoI, the correct fragment length is 422 bp, whereas the loss of the ITR results in a 326 bp fragment.
Vector backbone pscAAV-CAG-GFP (plasmid)
Selectable markers Ampicillin
Gene/insert name Bacteriophage P1 Cre recombinase; SV40 NLS.
Depositor|Developer Ikawa, Masahito |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. (Tatsuya Nakagawa et al. Exp. Anim., doi: 10.1538/expanim.24-0165. 2025) The RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with The University of Osaka.
Additional terms and conditions:
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit
academic purpose.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Tatsuya Nakagawa et al. Exp. Anim., doi: 10.1538/expanim.24-0165. 2025)。利用者が非営利目的の教育・研究以外の目的に用いる場合は、大阪大学と別途MTAを締結すること。
付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee
RDB20979 pscAAV-CAG-Cre DNA solution

How to cite this biological resource

Materials & Methods section:

The pscAAV-CAG-Cre was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20979).

Reference section:

Nakagawa, T., Emori, C., Ikawa, M., Trophectoderm-specific gene manipulation using adeno-associated viral vectors. Exp. Anim. 74 (3): 310-318 (2025). PMID 39805595. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Nakagawa, T., Trophectoderm-specific gene manipulation using adeno-associated viral vectors. Exp. Anim. 74 (3): 310-318 (2025). PMID 39805595. [PubMed] [Article] [RRC of NBRP]