Resource data sheet
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pClgn1.1 (#RDB19247)

Mammalian expression vector based on calmegin promoter.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)
GenBank Flat File Format open
SnapGene file download
Publication Ikawa, M., Dev. Biol. 240 (1): 254-261 (2001). PMID 11784061. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Mammalian expression vector based on calmegin promoter. For information about the calmegin promoter, see Watanabe, D. et al. FEBS Lett. 368 (3): 509-512, 1995.
Vector backbone pCAG1.1 (RDB19246) (plasmid)
Selectable markers Ampicillin
Growth conditions LB+Amp, 37℃
Gene/insert name mouse Clgn genomic
Depositor|Developer Ikawa, Masahito |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Ikawa, M. et al., Dev. Biol., 240 (1): 254-261, 2001). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Ikawa, M. et al., Dev. Biol., 240 (1): 254-261, 2001)。利用者が非営利目的の教育・研究以外の目的に用いる場合は、大阪大学と別途MTAを締結すること。

Catalog # Resource name Shipping form Fee
RDB19247 pClgn1.1 DNA solution


How to cite this biological resource

Materials & Methods section:

The pClgn1.1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19247).

Reference section:

Ikawa, M., Nakanishi, T., Yamada, S., Wada, I., Kominami, K., Tanaka, H., Nozaki, M., Nishimune, Y., Okabe, M., Calmegin is required for fertilin alpha/beta heterodimerization and sperm fertility. Dev. Biol. 240 (1): 254-261 (2001). PMID 11784061. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles

original Ikawa, M., Calmegin is required for fertilin alpha/beta heterodimerization and sperm fertility. Dev. Biol. 240 (1): 254-261 (2001). PMID 11784061. [PubMed] [Article] [RRC of NBRP]