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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAG_mRsph10b-PA

Expression vector of mouse Rsph10b.

Catalog number RDB17452
Resource name pCAG_mRsph10b-PA
Alternative name mouse Rsph10b on PA tagged pCAG vector
Clone info. Expression vector of mouse radial spoke head 10 homolog B (Rsph10b) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal.
Rsph10b was amplified from mouse testis cDNA. The PA tag is a dodecapeptide (GVAMPGAEDDVV) that is recognized by this high-affinity antibody. Expression was confirmed by the depositor with western blotting.
Vector backbone pCAGGS (plasmid)
Size of vector backbone 4.8 kb
Selectable markers Ampicillin
Gene/insert name mouse Rsph10b cDNA
Depositor|Developer Ikawa, Masahito | Miyata, Haruhiko |
 
Other clones in our bank mouse Rsph10b (NCBI Gene 75136) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Abbasi, F et al. J. Cell Sci. 131(19) jcs221648, 2018)
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RDB17452 pCAG_mRsph10b-PA DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Sequence (full) RDB17452zzk02.seq provided by the depositor

Original reference

original Abbasi, F., RSPH6A is required for sperm flagellum formation and male fertility in mice. J. Cell Sci. 131 (19). pii: jcs221648 (2018). PMID 30185526.

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17452_A9G9p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1
Region: insert 5'
Sequence file: RDB17452_A9G9a.seq check
>D06337A4_A9Gg_2_pAxCA_F1_E02_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGC CTTCTTCTTT TTNNNNCNGC TCCTGGGCAA CGTGCTGGTT
   61 ATTGTGCTGT CTCATCATTT TGGCAAATCT AGAGCCGCCA TGGTGAAAGA AAAGAAAAAA
  121 GCGGACAAGA AAGGAGATAA GTCAGCCCGC TCTCCCTCCT CCATTTCTGA TAACCCAGAA
  181 GCCTCGAAAC AAGATAGCAA TGCCAGTAAG CAAGAAGTGG CCCCCAGTGC TGTTGTCCCA
  241 GTCGTGGAAA CACCACTCAA GCAAGCCCCG AAAAGAGACT CAGTACAAAT GGAGCAGAGT
  301 GAAGAGGAGA CTCAGTACGA AGAGCCCATC CTGACCAAGC TCATAGTGGA AAGCTATGAA
  361 GGGGAGAAAG TCCGTGGACT GTATGAGGGA GAAGGCTTTG CTGTCTTTCA AGGCGGAAAC
  421 ACCTACCACG GTATGTTTTC AGAAGGACTG ATGCATGGAC AAGGCACTTA CATTTGGGCT
  481 GACGGATTGA AGTACGAGGG GGACTTTGTG AAGAATATCC CCATGAACCA CGGGGTGTAC
  541 ACATGGCCGG ACGGCAGCAC TTATGAAGGA GAGGTGACCA ATGGCATGAG GAATGGATTT
  601 GGTATGTTCA AGTGTGGCAC ACAGCCTGTG TCCTACATCG GCCACTGGTG TCATGGCAAG
  661 AGACATGGGA AGGGCTCCAT CTATTACAAT CAAGAGGGGA NCTCTTGGTA TGAAGGAGAC
  721 TGGGTATACA ACATCAAAAA GGGCTGGGGA ATAAGANGCT ATAAATCANG AAACATATAT
  781 GAAGGGCAGT GGGAGAACAA CATGCGCCAT GGGGAANGCA GAATGANGTG GCTCACNACA
  841 AACGAGGAGT ACACGGGCCA CTGGGGANAA NGGGANCCAG ANNGGCTTTG GAACNCACAC
  901 NTGGTTCCTA AAGAGAATCC CCAANTNTCA NTATCCCTTG AGAAANGAAT NNNTANGGGN
961 ANTNNNAAAT GGNTNNCGNC NNGNNNNNNG NNAATTTTNN TACNNNNNNN NNN
//
Primer: pCA-bgter
Region: PA tag, insert 3'
Sequence file: RDB17452_A9G9b.seq check
>D06337A4_A9Gg_2_pCA-bgter_F02_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNTT NNNTGNNNGA NGNNNNNNNN CTCNGTGGTA TTTGTGAGCC
   61 AGGGCATTGG CCACACCAGC CNCCACCTTC TGATAGGCAG CCTGCACCTG AGGAGTCTCG
  121 ATTTACACCA CATCATCTTC GGCACCTGGC ATGGCAACGC CGCTAGCCTC GAGGGCCGCG
  181 TCGACAAGCT TCTTCTTCTT CTTCTTGCCA GGGCTGGCCT TGCTAGTCTG GATGGAAGCC
  241 AGGTCCTCTT TAGGGGGGCT GGGCGTGAGA GGCTGAGAGG CTGGGATCTC CGATGGCTCC
  301 TTGAGCACAG TGATGTCAAG TTCGAAGTCT TGTTTCCTGG CCTCCTCTTC CCGTAGGATG
  361 TTCAGCCTGG CTTCTAGCTC TTCGTTCTCT ATCTGCCTCT GCTGAGCCAG CTCTGCTTCC
  421 TGGAGCTGAT TTTCCTTCAC TTTCTCCTTG AGCATCTCTT CACGTTTGTA GGCACTGAAG
  481 AGCACACTTA CAAAGAAGAC ATAGAGGTTG TTCACCCATA TGTTGAGTTT CTCTTTCTGT
  541 TCATCTTTGG GCTTTTCATA TTTCTCCTGT GGAGACATAA ACACCAGGCC TCTTCCTTGA
  601 GTCTTGAAGT TCATTTTGGA GACTTTGTCC TCACTCAGCG ACTTCTTGAG CTTGGGCTCT
  661 GACTGCCTGA TTTTACCATC AGGCAGAGCG CCTATCTTGT CTAAGGACGA CTTGGTGCTG
  721 CTGAAGTGGA CTTCGGAGTC ATGGCTTGTG ATTGCACTCG GGCTCCTGTG GANGCTCTGG
  781 TTCAGTATCG TCTGANTGCT GCCGTATCGG TTTGTAGTGA TGTCGTCATA CGGGNNTTTT
  841 AGATAGGNTC TAGTCATCTG GTCAAACATG CAGCAGANNN ANAANCATAA NANGNNNNNN
  901 AANANNTNNA GGAAANNCAG CTCAAGCTCG AANTTGCTGT CNNTTNNNTC NNNNNCNAAA
  961 GGGNTGTCNN NCNNNNTCAC ANNCATGAAN TTNGNNNNNA NNTNNNNNNN TNNNNNNNNN
1021 N
//
Primer: pUC-CAPbs_up_F
Region: lac pro, lacO, T3 pro, CMV en, CAG pro
Sequence file: RDB17452_A9G9c.seq check
>D06337A4_A9Gg_2_pUC-CAPbs_up_F_G02_20_ABI24.ab1
    1 NNNNNNNNNN NGNNNNNNNC NCNNCGCNNT AANNTGAGTT AGCTCACTCA TTAGGCACCC
   61 CAGGCTTTAN NNNNNNNNCT TCCGGCTCGT ATGTTGTGTG GAATTGTGAG CGGATAACAA
  121 TTTCACACAG GAAACAGCTA TGACCATGAT TACGCCAAGC GCGCAATTAA CCCTCACTAA
  181 AGGGAACAAA AGCTGGAGCT CATCGATACT CGACATTGAT TATTGACTAG TTATTAATAG
  241 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT
  301 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG
  361 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT
  421 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT
  481 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG
  541 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTCGAGGTG
  601 AGCCCCACGT TCTGCTTCAC TCTCCCCATC TCCCCCCCCT CCCCACCCCC AATTTTGTAT
661 TTATTTATTT TTTAATTATT TTGTGCAGCG ATGGGGGCNN NNGGGGGGNN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Abbasi, F., RSPH6A is required for sperm flagellum formation and male fertility in mice. J. Cell Sci. 131 (19). pii: jcs221648 (2018). PMID 30185526.

2021.08.20

GNP_filter3_RDBDEP_html_210618.pl