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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAG_mRsph10b-PA

Expression vector of mouse Rsph10b.

Catalog number RDB17452
Resource name pCAG_mRsph10b-PA
Alternative name mouse Rsph10b on PA tagged pCAG vector
Clone info. Expression vector of mouse radial spoke head 10 homolog B (Rsph10b) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal.
Comment Rsph10b was amplified from mouse testis cDNA. The PA tag is a dodecapeptide (GVAMPGAEDDVV) that is recognized by this high-affinity antibody. Expression was confirmed by the depositor with western blotting.
Vector backbone pCAGGS (plasmid)
Size of vector backbone 4.8 kb
Selectable markers Ampicillin
Gene/insert name mouse Rsph10b cDNA
Depositor|Developer Ikawa, Masahito | Miyata, Haruhiko |
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External Database
mouse Rsph10b

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Abbasi, F et al. J. Cell Sci. 131(19) jcs221648, 2018)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Abbasi, F et al. J. Cell Sci. 131(19) jcs221648, 2018)。
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          RDB17452 pCAG_mRsph10b-PA DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence (full) RDB17452zzk02.seq provided by the depositor

          Original reference

          original Abbasi, F., RSPH6A is required for sperm flagellum formation and male fertility in mice. J. Cell Sci. 131 (19). pii: jcs221648 (2018). PMID 30185526.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB17452_A9G9p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pAxCA_F1
          Region: insert 5'
          Sequence file: RDB17452_A9G9a.seq check
          >D06337A4_A9Gg_2_pAxCA_F1_E02_14_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNGC CTTCTTCTTT TTNNNNCNGC TCCTGGGCAA CGTGCTGGTT
             61 ATTGTGCTGT CTCATCATTT TGGCAAATCT AGAGCCGCCA TGGTGAAAGA AAAGAAAAAA
            121 GCGGACAAGA AAGGAGATAA GTCAGCCCGC TCTCCCTCCT CCATTTCTGA TAACCCAGAA
            181 GCCTCGAAAC AAGATAGCAA TGCCAGTAAG CAAGAAGTGG CCCCCAGTGC TGTTGTCCCA
            241 GTCGTGGAAA CACCACTCAA GCAAGCCCCG AAAAGAGACT CAGTACAAAT GGAGCAGAGT
            301 GAAGAGGAGA CTCAGTACGA AGAGCCCATC CTGACCAAGC TCATAGTGGA AAGCTATGAA
            361 GGGGAGAAAG TCCGTGGACT GTATGAGGGA GAAGGCTTTG CTGTCTTTCA AGGCGGAAAC
            421 ACCTACCACG GTATGTTTTC AGAAGGACTG ATGCATGGAC AAGGCACTTA CATTTGGGCT
            481 GACGGATTGA AGTACGAGGG GGACTTTGTG AAGAATATCC CCATGAACCA CGGGGTGTAC
            541 ACATGGCCGG ACGGCAGCAC TTATGAAGGA GAGGTGACCA ATGGCATGAG GAATGGATTT
            601 GGTATGTTCA AGTGTGGCAC ACAGCCTGTG TCCTACATCG GCCACTGGTG TCATGGCAAG
            661 AGACATGGGA AGGGCTCCAT CTATTACAAT CAAGAGGGGA NCTCTTGGTA TGAAGGAGAC
            721 TGGGTATACA ACATCAAAAA GGGCTGGGGA ATAAGANGCT ATAAATCANG AAACATATAT
            781 GAAGGGCAGT GGGAGAACAA CATGCGCCAT GGGGAANGCA GAATGANGTG GCTCACNACA
            841 AACGAGGAGT ACACGGGCCA CTGGGGANAA NGGGANCCAG ANNGGCTTTG GAACNCACAC
            901 NTGGTTCCTA AAGAGAATCC CCAANTNTCA NTATCCCTTG AGAAANGAAT NNNTANGGGN
          961 ANTNNNAAAT GGNTNNCGNC NNGNNNNNNG NNAATTTTNN TACNNNNNNN NNN
          //
          Primer: pCA-bgter
          Region: PA tag, insert 3'
          Sequence file: RDB17452_A9G9b.seq check
          >D06337A4_A9Gg_2_pCA-bgter_F02_17_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNTT NNNTGNNNGA NGNNNNNNNN CTCNGTGGTA TTTGTGAGCC
             61 AGGGCATTGG CCACACCAGC CNCCACCTTC TGATAGGCAG CCTGCACCTG AGGAGTCTCG
            121 ATTTACACCA CATCATCTTC GGCACCTGGC ATGGCAACGC CGCTAGCCTC GAGGGCCGCG
            181 TCGACAAGCT TCTTCTTCTT CTTCTTGCCA GGGCTGGCCT TGCTAGTCTG GATGGAAGCC
            241 AGGTCCTCTT TAGGGGGGCT GGGCGTGAGA GGCTGAGAGG CTGGGATCTC CGATGGCTCC
            301 TTGAGCACAG TGATGTCAAG TTCGAAGTCT TGTTTCCTGG CCTCCTCTTC CCGTAGGATG
            361 TTCAGCCTGG CTTCTAGCTC TTCGTTCTCT ATCTGCCTCT GCTGAGCCAG CTCTGCTTCC
            421 TGGAGCTGAT TTTCCTTCAC TTTCTCCTTG AGCATCTCTT CACGTTTGTA GGCACTGAAG
            481 AGCACACTTA CAAAGAAGAC ATAGAGGTTG TTCACCCATA TGTTGAGTTT CTCTTTCTGT
            541 TCATCTTTGG GCTTTTCATA TTTCTCCTGT GGAGACATAA ACACCAGGCC TCTTCCTTGA
            601 GTCTTGAAGT TCATTTTGGA GACTTTGTCC TCACTCAGCG ACTTCTTGAG CTTGGGCTCT
            661 GACTGCCTGA TTTTACCATC AGGCAGAGCG CCTATCTTGT CTAAGGACGA CTTGGTGCTG
            721 CTGAAGTGGA CTTCGGAGTC ATGGCTTGTG ATTGCACTCG GGCTCCTGTG GANGCTCTGG
            781 TTCAGTATCG TCTGANTGCT GCCGTATCGG TTTGTAGTGA TGTCGTCATA CGGGNNTTTT
            841 AGATAGGNTC TAGTCATCTG GTCAAACATG CAGCAGANNN ANAANCATAA NANGNNNNNN
            901 AANANNTNNA GGAAANNCAG CTCAAGCTCG AANTTGCTGT CNNTTNNNTC NNNNNCNAAA
            961 GGGNTGTCNN NCNNNNTCAC ANNCATGAAN TTNGNNNNNA NNTNNNNNNN TNNNNNNNNN
          1021 N
          //
          Primer: pUC-CAPbs_up_F
          Region: lac pro, lacO, T3 pro, CMV en, CAG pro
          Sequence file: RDB17452_A9G9c.seq check
          >D06337A4_A9Gg_2_pUC-CAPbs_up_F_G02_20_ABI24.ab1
              1 NNNNNNNNNN NGNNNNNNNC NCNNCGCNNT AANNTGAGTT AGCTCACTCA TTAGGCACCC
             61 CAGGCTTTAN NNNNNNNNCT TCCGGCTCGT ATGTTGTGTG GAATTGTGAG CGGATAACAA
            121 TTTCACACAG GAAACAGCTA TGACCATGAT TACGCCAAGC GCGCAATTAA CCCTCACTAA
            181 AGGGAACAAA AGCTGGAGCT CATCGATACT CGACATTGAT TATTGACTAG TTATTAATAG
            241 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT
            301 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG
            361 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT
            421 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT
            481 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG
            541 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTCGAGGTG
            601 AGCCCCACGT TCTGCTTCAC TCTCCCCATC TCCCCCCCCT CCCCACCCCC AATTTTGTAT
          661 TTATTTATTT TTTAATTATT TTGTGCAGCG ATGGGGGCNN NNGGGGGGNN NNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Abbasi, F., RSPH6A is required for sperm flagellum formation and male fertility in mice. J. Cell Sci. 131 (19). pii: jcs221648 (2018). PMID 30185526.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl