Expression vector of mouse Rsph10b.
Alternative name | mouse Rsph10b on PA tagged pCAG vector |
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Clone info. | Expression vector of mouse radial spoke head 10 homolog B (Rsph10b) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal. |
Comment | Rsph10b was amplified from mouse testis cDNA. The PA tag is a dodecapeptide (GVAMPGAEDDVV) that is recognized by this high-affinity antibody. Expression was confirmed by the depositor with western blotting. |
Vector backbone | pCAGGS (plasmid) |
Size of vector backbone | 4.8 kb |
Selectable markers | Ampicillin |
Gene/insert name | mouse Rsph10b cDNA |
Depositor|Developer | Ikawa, Masahito | Miyata, haruhiko | |
Sequence (full) ![]() |
RDB17452zzk02.seq provided by the depositor |
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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Abbasi, F et al. J. Cell Sci. 131(19) jcs221648, 2018) |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Abbasi, F et al. J. Cell Sci. 131(19) jcs221648, 2018)。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB17452 | pCAG_mRsph10b-PA | DNA solution |
Materials & Methods section:
The pCAG_mRsph10b-PA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17452). |
Reference section:
Abbasi, F., Miyata, H., Shimada, K., Morohoshi, A., Nozawa, K., Matsumura, T., Xu, Z., Pratiwi, P., Ikawa, M., RSPH6A is required for sperm flagellum formation and male fertility in mice. J. Cell Sci. 131 (19). pii: jcs221648 (2018). PMID 30185526. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB17452_A9G9p1-1.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: pAxCA_F1 (Pr0164) Region: insert 5' Sequence file: RDB17452_A9G9a.seq ![]() |
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>D06337A4_A9Gg_2_pAxCA_F1_E02_14_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNGC CTTCTTCTTT TTNNNNCNGC TCCTGGGCAA CGTGCTGGTT 61 ATTGTGCTGT CTCATCATTT TGGCAAATCT AGAGCCGCCA TGGTGAAAGA AAAGAAAAAA 121 GCGGACAAGA AAGGAGATAA GTCAGCCCGC TCTCCCTCCT CCATTTCTGA TAACCCAGAA 181 GCCTCGAAAC AAGATAGCAA TGCCAGTAAG CAAGAAGTGG CCCCCAGTGC TGTTGTCCCA 241 GTCGTGGAAA CACCACTCAA GCAAGCCCCG AAAAGAGACT CAGTACAAAT GGAGCAGAGT 301 GAAGAGGAGA CTCAGTACGA AGAGCCCATC CTGACCAAGC TCATAGTGGA AAGCTATGAA 361 GGGGAGAAAG TCCGTGGACT GTATGAGGGA GAAGGCTTTG CTGTCTTTCA AGGCGGAAAC 421 ACCTACCACG GTATGTTTTC AGAAGGACTG ATGCATGGAC AAGGCACTTA CATTTGGGCT 481 GACGGATTGA AGTACGAGGG GGACTTTGTG AAGAATATCC CCATGAACCA CGGGGTGTAC 541 ACATGGCCGG ACGGCAGCAC TTATGAAGGA GAGGTGACCA ATGGCATGAG GAATGGATTT 601 GGTATGTTCA AGTGTGGCAC ACAGCCTGTG TCCTACATCG GCCACTGGTG TCATGGCAAG 661 AGACATGGGA AGGGCTCCAT CTATTACAAT CAAGAGGGGA NCTCTTGGTA TGAAGGAGAC 721 TGGGTATACA ACATCAAAAA GGGCTGGGGA ATAAGANGCT ATAAATCANG AAACATATAT 781 GAAGGGCAGT GGGAGAACAA CATGCGCCAT GGGGAANGCA GAATGANGTG GCTCACNACA 841 AACGAGGAGT ACACGGGCCA CTGGGGANAA NGGGANCCAG ANNGGCTTTG GAACNCACAC 901 NTGGTTCCTA AAGAGAATCC CCAANTNTCA NTATCCCTTG AGAAANGAAT NNNTANGGGN 961 ANTNNNAAAT GGNTNNCGNC NNGNNNNNNG NNAATTTTNN TACNNNNNNN NNN // |
Primer: pCA-bgter (Pr0066) Region: PA tag, insert 3' Sequence file: RDB17452_A9G9b.seq ![]() |
>D06337A4_A9Gg_2_pCA-bgter_F02_17_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNTT NNNTGNNNGA NGNNNNNNNN CTCNGTGGTA TTTGTGAGCC 61 AGGGCATTGG CCACACCAGC CNCCACCTTC TGATAGGCAG CCTGCACCTG AGGAGTCTCG 121 ATTTACACCA CATCATCTTC GGCACCTGGC ATGGCAACGC CGCTAGCCTC GAGGGCCGCG 181 TCGACAAGCT TCTTCTTCTT CTTCTTGCCA GGGCTGGCCT TGCTAGTCTG GATGGAAGCC 241 AGGTCCTCTT TAGGGGGGCT GGGCGTGAGA GGCTGAGAGG CTGGGATCTC CGATGGCTCC 301 TTGAGCACAG TGATGTCAAG TTCGAAGTCT TGTTTCCTGG CCTCCTCTTC CCGTAGGATG 361 TTCAGCCTGG CTTCTAGCTC TTCGTTCTCT ATCTGCCTCT GCTGAGCCAG CTCTGCTTCC 421 TGGAGCTGAT TTTCCTTCAC TTTCTCCTTG AGCATCTCTT CACGTTTGTA GGCACTGAAG 481 AGCACACTTA CAAAGAAGAC ATAGAGGTTG TTCACCCATA TGTTGAGTTT CTCTTTCTGT 541 TCATCTTTGG GCTTTTCATA TTTCTCCTGT GGAGACATAA ACACCAGGCC TCTTCCTTGA 601 GTCTTGAAGT TCATTTTGGA GACTTTGTCC TCACTCAGCG ACTTCTTGAG CTTGGGCTCT 661 GACTGCCTGA TTTTACCATC AGGCAGAGCG CCTATCTTGT CTAAGGACGA CTTGGTGCTG 721 CTGAAGTGGA CTTCGGAGTC ATGGCTTGTG ATTGCACTCG GGCTCCTGTG GANGCTCTGG 781 TTCAGTATCG TCTGANTGCT GCCGTATCGG TTTGTAGTGA TGTCGTCATA CGGGNNTTTT 841 AGATAGGNTC TAGTCATCTG GTCAAACATG CAGCAGANNN ANAANCATAA NANGNNNNNN 901 AANANNTNNA GGAAANNCAG CTCAAGCTCG AANTTGCTGT CNNTTNNNTC NNNNNCNAAA 961 GGGNTGTCNN NCNNNNTCAC ANNCATGAAN TTNGNNNNNA NNTNNNNNNN TNNNNNNNNN 1021 N // |
Primer: pUC-CAPbs_up_F (Pr0536) Region: lac pro, lacO, T3 pro, CMV en, CAG pro Sequence file: RDB17452_A9G9c.seq ![]() |
>D06337A4_A9Gg_2_pUC-CAPbs_up_F_G02_20_ABI24.ab1 1 NNNNNNNNNN NGNNNNNNNC NCNNCGCNNT AANNTGAGTT AGCTCACTCA TTAGGCACCC 61 CAGGCTTTAN NNNNNNNNCT TCCGGCTCGT ATGTTGTGTG GAATTGTGAG CGGATAACAA 121 TTTCACACAG GAAACAGCTA TGACCATGAT TACGCCAAGC GCGCAATTAA CCCTCACTAA 181 AGGGAACAAA AGCTGGAGCT CATCGATACT CGACATTGAT TATTGACTAG TTATTAATAG 241 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 301 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 361 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 421 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 481 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 541 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTCGAGGTG 601 AGCCCCACGT TCTGCTTCAC TCTCCCCATC TCCCCCCCCT CCCCACCCCC AATTTTGTAT 661 TTATTTATTT TTTAATTATT TTGTGCAGCG ATGGGGGCNN NNGGGGGGNN NNNNNNNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Abbasi, F., RSPH6A is required for sperm flagellum formation and male fertility in mice. J. Cell Sci. 131 (19). pii: jcs221648 (2018). PMID 30185526. [link to RRC of NBRP] |
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