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pCAG_mRsph10b-PA

Expression vector of mouse Rsph10b.

Catalog number RDB17452
Resource name pCAG_mRsph10b-PA
Alternative name mouse Rsph10b on PA tagged pCAG vector
Clone info. Expression vector of mouse radial spoke head 10 homolog B (Rsph10b) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal.
Rsph10b was amplified from mouse testis cDNA. The PA tag is a dodecapeptide (GVAMPGAEDDVV) that is recognized by this high-affinity antibody. Expression was confirmed by the depositor with western blotting.
Vector backbone pCAGGS (plasmid)
Size of vector backbone 4.8 kb
Selectable markers Ampicillin
Gene/insert name mouse Rsph10b cDNA
Depositor Ikawa, Masahito | Miyata, Haruhiko |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Abbasi, F et al. J. Cell Sci. 131(19) jcs221648, 2018)
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RDB17452 pCAG_mRsph10b-PA Under QC test. Please contact us. DNA solution

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References and tips

Electronic file

Original reference

original Abbasi, F., RSPH6A is required for sperm flagellum formation and male fertility in mice. J. Cell Sci. 131 (19). pii: jcs221648 (2018). PMID 30185526.

Further references such as user reports and related articles (go to bottom)

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Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

original Abbasi, F., RSPH6A is required for sperm flagellum formation and male fertility in mice. J. Cell Sci. 131 (19). pii: jcs221648 (2018). PMID 30185526.

2019.11.05

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