Resource data sheet
DNA Bank Top

pClgn-Lrrc23-1D4 (#RDB19440)

Expression vector of mouse Lrrc23 tagged with 1D4 at C-terminus, driven by Calmergin promoter.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Zhang, X., J. Cell Sci. 134 (20): jcs259381 (2021). PMID 34585727. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Expression vector of mouse leucine rich repeat containing 23 (Lrrc23) tagged with 1D4 at C-terminus, Calmergin (Clgn) promoter, poly (A) signal. See Fig.8A of Zhang, X. et al. J. Cell Sci. jcs.259381, 2021 (134 (20)).
Comment Expression was confirmed by the depositor with immunoblotting
Vector backbone pClgn (plasmid)
Selectable markers Ampicillin
Growth conditions LB+Amp, 37oC
Gene/insert name mouse Lrrc23 cDNA
Depositor|Developer Ikawa, Masahito | Lu, Yonggang |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Zhang, X. et al. J. Cell Sci. 134(20): jcs.259381, 2021). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Zhang, X. et al., J. Cell Sci. 134(20): jcs.259381, 2021)。利用者が非営利目的の教育・研究以外の目的に用いる場合は、大阪大学と別途MTAを締結すること。

Catalog # Resource name Shipping form Fee
RDB19440 pClgn-Lrrc23-1D4 DNA solution

How to cite this biological resource

Materials & Methods section:

The pClgn-Lrrc23-1D4 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19440).

Reference section:

Zhang, X., Sun, J., Lu, Y., Zhang, J,. Shimada, K., Noda, T., Zhao, S., Koyano, T., Matsuyama, M., Zhou, S., Wu, J., Ikawa, M., Liu, M., LRRC23 is a conserved component of the radial spoke that is necessary for sperm motility and male fertility in mice. J. Cell Sci. 134 (20): jcs259381 (2021). PMID 34585727. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Zhang, X., LRRC23 is a conserved component of the radial spoke that is necessary for sperm motility and male fertility in mice. J. Cell Sci. 134 (20): jcs259381 (2021). PMID 34585727. [PubMed] [Article] [RRC of NBRP]