Expression vector of mouse Spata33 with PA-tag. I81A mutation is introduced in SPATA33 (PQAIIT).
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Clone info. | Expression vector of mouse spermatogenesis associated 33 (Spata33) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal. I81A mutation is introduced in SPATA33 (PQAIIT). |
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Comment | Expression was confirmed by the depositor with western blotting. |
Vector backbone | pCAGGS (plasmid) |
Size of vector backbone | 5.2 kb |
Selectable markers | Ampicillin |
Gene/insert name | mouse Spata33 cDNA |
Depositor|Developer | Ikawa, Masahito | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() MTA, for use for for-profit purpose [Word ![]() Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Miyata, H. et al., Proc. Natl. Acad. Sci. USA. 118 (35): e2106673118, 2021). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested. |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 提供同意書 (MTA, 営利目的用)[Word ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Miyata, H. et al., Proc. Natl. Acad. Sci. USA. 118 (35): e2106673118, 2021)。利用者が非営利目的の教育・研究以外の目的に用いる場合は、大阪大学と別途MTAを締結すること。 MTAに書く付加的使用条件: CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB19400 | pCAG-mSpata33 (I81A)-PA | DNA solution |
Materials & Methods section:
The pCAG-mSpata33 (I81A)-PA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19400). |
Reference section:
Miyata, H., Oura, S., Morohoshi, A., Shimada, K., Mashiko, D., Oyama, Y., Kaneda, Y., Matsumura, T., Abbasi, F., Ikawa, M., SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice. Proc. Natl. Acad. Sci. U.S.A. 118 (35): e2106673118 (2021). PMID 34446558. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Original, user report and related articles
original | Miyata, H., SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice. Proc. Natl. Acad. Sci. U.S.A. 118 (35): e2106673118 (2021). PMID 34446558. [link to RRC of NBRP] |
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