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pCAG-hPPP3CA-FLAG

Expression vector of human PPP3CA with FLAG-tag.

Catalog number RDB19403
Resource name pCAG-hPPP3CA-FLAG
Clone info. Expression vector of human protein phosphatase 3, catalytic subunit, alpha isoform (PPP3CA) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.
Comment Expression was confirmed by the depositor with western blotting.
Vector backbone pCAGGS (plasmid)
Size of vector backbone 5.2 kb
Selectable markers Ampicillin
Gene/insert name human PPP3CA cDNA
Depositor|Developer Ikawa, Masahito |
 
Sequence (full) RDB19403hts01.seqcheck assembled from experimentally sequenced data
Test sheet RDB19403_B1Kbp1-1.pdf 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Miyata, H. et al., Proc. Natl. Acad. Sci. USA. 118 (35): e2106673118, 2021). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
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MTA, for use for not-for-profit academic purpose [Word]
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Please visit Information of Request for Distribution.[link] 
提供案内 (日本国内) [open/close]

提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Miyata, H. et al., Proc. Natl. Acad. Sci. USA. 118 (35): e2106673118, 2021)。利用者が非営利目的の教育・研究以外の目的に用いる場合は、大阪大学と別途MTAを締結すること。
付加的提供条件:
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
提供依頼
提供依頼書 [Word]
提供同意書 (MTA, 非営利学術目的用)[Word]
提供同意書 (MTA, 営利目的用)[Word]
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19403 pCAG-hPPP3CA-FLAG DNA solution


How to cite this biological resource

Materials & Methods section:

The pCAG-hPPP3CA-FLAG was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19403).

Reference section:

Miyata, H., Oura, S., Morohoshi, A., Shimada, K., Mashiko, D., Oyama, Y., Kaneda, Y., Matsumura, T., Abbasi, F., Ikawa, M., SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice. Proc. Natl. Acad. Sci. U.S.A. 118 (35): e2106673118 (2021). PMID 34446558. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles

original Miyata, H., SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice. Proc. Natl. Acad. Sci. U.S.A. 118 (35): e2106673118 (2021). PMID 34446558. [link to RRC of NBRP]