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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAG_mRsph1-PA

Expression vector of mouse Rsph1.

Catalog number RDB17449
Resource name pCAG_mRsph1-PA
Alternative name mouse Rsph1 on PA tagged pCAG vector
Clone info. Expression vector of mouse radial spoke head 1 homolog (Rsph1) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal.
Rsph1 was amplified from mouse testis cDNA. The PA tag is a dodecapeptide (GVAMPGAEDDVV) that is recognized by this high-affinity antibody. Expression was confirmed by the depositor with western blotting.
Vector backbone pCAGGS (plasmid)
Size of vector backbone 4.8 kb
Selectable markers Ampicillin
Gene/insert name mouse Rsph1 cDNA
Depositor|Developer Ikawa, Masahito | Miyata, Haruhiko |
 
Other clones in our bank mouse Rsph1 (NCBI Gene 22092) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Abbasi, F et al. J. Cell Sci. 131(19) jcs221648, 2018)
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RDB17449 pCAG_mRsph1-PA DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Sequence (full) RDB17449zzk02.seq provided by the depositor

Original reference

original Abbasi, F., RSPH6A is required for sperm flagellum formation and male fertility in mice. J. Cell Sci. 131 (19). pii: jcs221648 (2018). PMID 30185526.

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17449_A9G9p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1
Region: CAG pro, insert 5'
Sequence file: RDB17449_A9G9a.seq check
>D06337A1_A9Gg_2_pAxCA_F1_A01_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGCC TTCTTCTTTT TCCNNCNGCT CCTGGGCAAC GTGCTGGTTA
   61 TTGTGCTGTC TCATCATTTT GGCAAATCTA GAATCGATGC GGCCGCAGTC GACAAGCTTG
  121 CCGCCATGTC GGACCTGGGC TCTGAGGAGT TAGAGGAGGA GGGAGAGAAC GACCTTGGGG
  181 AGTACGAAGG GGAGCGCAAT GAGGTGGGAG AACGACACGG ACATGGGAAA GCACGACTGC
  241 CCAACGGGGA CACATATGAA GGAAGCTATG AGTTTGGAAA AAGACATGGC CAGGGGACTT
  301 ACAAATTTAA GAATGGGGCC CGGTACACCG GAGACTATGT CAAAAATAAA AAGCACGGCC
  361 AAGGCACCTT TATCTATCCA GATGGATCCA GATATGAAGG GGAGTGGGCT GATGACCAGA
  421 GGCACGGCCA AGGCGTGTAC TACTATGTCA ACAATGACAC CTACACAGGG GAGTGGTTCA
  481 ATCATCAAAG GCACGGGCAA GGCACCTACC TCTACGCAGA GACCGGCAGT AAGTATGTCG
  541 GTACCTGGGT GCACGGACAG CAGGAGGGTG CTGCCGAGCT CATCCACCTA AACCACAGGT
  601 ACCAGGGCAA GTTCATGAAC AAAAATCCTG TGGGCCCTGG AAAGTACGTA TTTGATATTG
  661 GATGCGAACA GCACGGTGAA TATCGCCTCA CAGATACGGA AAGAGGAGAA GAGGAGGANG
  721 AGGAAGAGAC ATTAGTGAAC ATCGTTCCNA AATGGAAAGC TCTCAACATC ACAGAANTGG
  781 CCCTGTGGAC TCCAACCCTC TCCGANGANC AGCCTCCCCC TGANGGCCNA NGCCAGGANG
  841 AACCGCANGG NCTTACTGGC GTNNNTGANC CCTCNNANNA NATCCAGGCA GAANNNNTTT
  901 GANGGCGANC TGNANCCNAN GGGGGGCCGA NGAAGANGNA NANNCGTNCA GGNNNNANAN
961 CCAGGANNAC AGCTACNANN TNNANNANGG NANCTNNNAN NTTNNNNNNN NNN
//
Primer: pCA-bgter
Region: PA tag, insert 3'
Sequence file: RDB17449_A9G9b.seq check
>D06337A1_A9Gg_2_pCA-bgter_B01_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNTT TGNNNNANGN NNNNNNCTCN GTGGTATTTG TGAGCCAGGG
   61 CATTGGCCAC ACCAGCCACC ACCTTCTGAT AGGCAGCCTG CACCTGAGGA GTCTCGAGCT
  121 AGGCAGGCGC CACTTGGCTG GTCTCTGTGC CCCCGCCCCC GATCACCACA TCATCTTCGG
  181 CACCTGGCAT GGCAACGCCG ATATCGAATT CATCCTGGAG GTCTGACGGT TCTTCATCAA
  241 AGTTCAAGTT TCCCTGGTCT ATGTCGTAGC TGTTCTCCTG GCTCTCCTGC CTGAACGTGT
  301 CTACGTCTTC ATCGGCCCCC CTCGGCTCCA GCTCGCCCTC AAAACCTTCT GCCTGGATGT
  361 CTTCTGAGGG GTCACCCACG CCAGTAAGTC CCTGCGGTTC CTCCTGGCCT TGGCCCTCAG
  421 GGGGAGGCTG CTCCTCGGAG AGGGTTGGAG TCCACAGGGC CAATTCTGTG ATGTTGAGAG
  481 CTTTCCATTT TGGAACGATG TTCACTAATG TCTCTTCCTC CTCCTCCTCT TCTCCTCTTT
  541 CCGTATCTGT GAGGCGATAT TCACCGTGCT GTTCGCATCN AATATCAAAT ACGTACTTTC
  601 CAGGGCCCAC AGGANTTTTG TTCATGAACT TGCCCTGGTA CCTGTGGTTT ANGTGGANGA
  661 GCTCGGCAGC ACCCTCCTGC TGTCCGTGCA CNCAGGTACC GACATACTTA CTGCCGGTCT
  721 CTGCGTANAG GTANGTGCCT TGNCCGNGCC TTTGANGANT NNNNNNCTCC CCTGNGTNNN
  781 TGTCATNGTT GANNTAGNAN TACACGCCTN GGCCGNGCNN CTNGTCATCA NCCCNNTNCC
  841 NTNNNTATCN NNNNNNNTCN NNNNANNNAA NNNNNNNNNG NNNNNNNTTT TNANTTTNGA
901 NNNANNCTNN NNNGNNNNNG GNNCNNNNNN NNNANNTNNN NNNNNCNNNN NNNNNN
//
Primer: pUC-CAPbs_up_F
Region: lac pro, lacO, T3 pro, CMV en, CAG pro
Sequence file: RDB17449_A9G9c.seq check
>D06337A1_A9Gg_2_pUC-CAPbs_up_F_C01_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNANNNN NNNNNNNCAN TTAANGTGAG TTAGCTCNCT NNTTAGGCAC
   61 CCCAGGCTTT ACACTNNNNN CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC
  121 AATTTCACAC AGGAAACAGC TATGACCATG ATTACGCCAA GCGCGCAATT AACCCTCACT
  181 AAAGGGAACA AAAGCTGGAG CTCATCGATA CTCGACATTG ATTATTGACT AGTTATTAAT
  241 AGTAATCAAT TACGGGGTCA TTAGTTCATA GCCCATATAT GGAGTTCCGC GTTACATAAC
  301 TTACGGTAAA TGGCCCGCCT GGCTGACCGC CCAACGACCC CCGCCCATTG ACGTCAATAA
  361 TGACGTATGT TCCCATAGTA ACGCCAATAG GGACTTTCCA TTGACGTCAA TGGGTGGAGT
  421 ATTTACGGTA AACTGCCCAC TTGGCAGTAC ATCAAGTGTA TCATATGCCA AGTACGCCCC
  481 CTATTGACGT CAATGACGGT AAATGGCCCG CCTGGCATTA TGCCCAGTAC ATGACCTTAT
  541 GGGACTTTCC TACTTGGCAG TACATCTACG TATTAGTCAT CGCTATTACC ATGGTCGAGG
  601 TGAGCCCCAC GTTCTGCTTC ACTCTCCCCA TCTCCCCCCC CTCCCCACCC CCAATTTTGT
  661 ATTTATTTAT TTTTTAATTA TTTTGTGCAG CGATGGGGGC NNNGGGGGGG GNNNNNNNNN
721 N
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Abbasi, F., RSPH6A is required for sperm flagellum formation and male fertility in mice. J. Cell Sci. 131 (19). pii: jcs221648 (2018). PMID 30185526.

2021.08.20

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