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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAG_mRsph1-PA

Expression vector of mouse Rsph1.

Catalog number RDB17449
Resource name pCAG_mRsph1-PA
Alternative name mouse Rsph1 on PA tagged pCAG vector
Clone info. Expression vector of mouse radial spoke head 1 homolog (Rsph1) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal.
Comment Rsph1 was amplified from mouse testis cDNA. The PA tag is a dodecapeptide (GVAMPGAEDDVV) that is recognized by this high-affinity antibody. Expression was confirmed by the depositor with western blotting.
Vector backbone pCAGGS (plasmid)
Size of vector backbone 4.8 kb
Selectable markers Ampicillin
Gene/insert name mouse Rsph1 cDNA
Depositor|Developer Ikawa, Masahito | Miyata, Haruhiko |
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External Database
mouse Rsph1

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Abbasi, F et al. J. Cell Sci. 131(19) jcs221648, 2018)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Abbasi, F et al. J. Cell Sci. 131(19) jcs221648, 2018)。
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          RDB17449 pCAG_mRsph1-PA DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence (full) RDB17449zzk02.seq provided by the depositor

          Original reference

          original Abbasi, F., RSPH6A is required for sperm flagellum formation and male fertility in mice. J. Cell Sci. 131 (19). pii: jcs221648 (2018). PMID 30185526.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB17449_A9G9p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pAxCA_F1
          Region: CAG pro, insert 5'
          Sequence file: RDB17449_A9G9a.seq check
          >D06337A1_A9Gg_2_pAxCA_F1_A01_01_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNGCC TTCTTCTTTT TCCNNCNGCT CCTGGGCAAC GTGCTGGTTA
             61 TTGTGCTGTC TCATCATTTT GGCAAATCTA GAATCGATGC GGCCGCAGTC GACAAGCTTG
            121 CCGCCATGTC GGACCTGGGC TCTGAGGAGT TAGAGGAGGA GGGAGAGAAC GACCTTGGGG
            181 AGTACGAAGG GGAGCGCAAT GAGGTGGGAG AACGACACGG ACATGGGAAA GCACGACTGC
            241 CCAACGGGGA CACATATGAA GGAAGCTATG AGTTTGGAAA AAGACATGGC CAGGGGACTT
            301 ACAAATTTAA GAATGGGGCC CGGTACACCG GAGACTATGT CAAAAATAAA AAGCACGGCC
            361 AAGGCACCTT TATCTATCCA GATGGATCCA GATATGAAGG GGAGTGGGCT GATGACCAGA
            421 GGCACGGCCA AGGCGTGTAC TACTATGTCA ACAATGACAC CTACACAGGG GAGTGGTTCA
            481 ATCATCAAAG GCACGGGCAA GGCACCTACC TCTACGCAGA GACCGGCAGT AAGTATGTCG
            541 GTACCTGGGT GCACGGACAG CAGGAGGGTG CTGCCGAGCT CATCCACCTA AACCACAGGT
            601 ACCAGGGCAA GTTCATGAAC AAAAATCCTG TGGGCCCTGG AAAGTACGTA TTTGATATTG
            661 GATGCGAACA GCACGGTGAA TATCGCCTCA CAGATACGGA AAGAGGAGAA GAGGAGGANG
            721 AGGAAGAGAC ATTAGTGAAC ATCGTTCCNA AATGGAAAGC TCTCAACATC ACAGAANTGG
            781 CCCTGTGGAC TCCAACCCTC TCCGANGANC AGCCTCCCCC TGANGGCCNA NGCCAGGANG
            841 AACCGCANGG NCTTACTGGC GTNNNTGANC CCTCNNANNA NATCCAGGCA GAANNNNTTT
            901 GANGGCGANC TGNANCCNAN GGGGGGCCGA NGAAGANGNA NANNCGTNCA GGNNNNANAN
          961 CCAGGANNAC AGCTACNANN TNNANNANGG NANCTNNNAN NTTNNNNNNN NNN
          //
          Primer: pCA-bgter
          Region: PA tag, insert 3'
          Sequence file: RDB17449_A9G9b.seq check
          >D06337A1_A9Gg_2_pCA-bgter_B01_04_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNTT TGNNNNANGN NNNNNNCTCN GTGGTATTTG TGAGCCAGGG
             61 CATTGGCCAC ACCAGCCACC ACCTTCTGAT AGGCAGCCTG CACCTGAGGA GTCTCGAGCT
            121 AGGCAGGCGC CACTTGGCTG GTCTCTGTGC CCCCGCCCCC GATCACCACA TCATCTTCGG
            181 CACCTGGCAT GGCAACGCCG ATATCGAATT CATCCTGGAG GTCTGACGGT TCTTCATCAA
            241 AGTTCAAGTT TCCCTGGTCT ATGTCGTAGC TGTTCTCCTG GCTCTCCTGC CTGAACGTGT
            301 CTACGTCTTC ATCGGCCCCC CTCGGCTCCA GCTCGCCCTC AAAACCTTCT GCCTGGATGT
            361 CTTCTGAGGG GTCACCCACG CCAGTAAGTC CCTGCGGTTC CTCCTGGCCT TGGCCCTCAG
            421 GGGGAGGCTG CTCCTCGGAG AGGGTTGGAG TCCACAGGGC CAATTCTGTG ATGTTGAGAG
            481 CTTTCCATTT TGGAACGATG TTCACTAATG TCTCTTCCTC CTCCTCCTCT TCTCCTCTTT
            541 CCGTATCTGT GAGGCGATAT TCACCGTGCT GTTCGCATCN AATATCAAAT ACGTACTTTC
            601 CAGGGCCCAC AGGANTTTTG TTCATGAACT TGCCCTGGTA CCTGTGGTTT ANGTGGANGA
            661 GCTCGGCAGC ACCCTCCTGC TGTCCGTGCA CNCAGGTACC GACATACTTA CTGCCGGTCT
            721 CTGCGTANAG GTANGTGCCT TGNCCGNGCC TTTGANGANT NNNNNNCTCC CCTGNGTNNN
            781 TGTCATNGTT GANNTAGNAN TACACGCCTN GGCCGNGCNN CTNGTCATCA NCCCNNTNCC
            841 NTNNNTATCN NNNNNNNTCN NNNNANNNAA NNNNNNNNNG NNNNNNNTTT TNANTTTNGA
          901 NNNANNCTNN NNNGNNNNNG GNNCNNNNNN NNNANNTNNN NNNNNCNNNN NNNNNN
          //
          Primer: pUC-CAPbs_up_F
          Region: lac pro, lacO, T3 pro, CMV en, CAG pro
          Sequence file: RDB17449_A9G9c.seq check
          >D06337A1_A9Gg_2_pUC-CAPbs_up_F_C01_07_ABI24.ab1
              1 NNNNNNNNNN NNNNNANNNN NNNNNNNCAN TTAANGTGAG TTAGCTCNCT NNTTAGGCAC
             61 CCCAGGCTTT ACACTNNNNN CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC
            121 AATTTCACAC AGGAAACAGC TATGACCATG ATTACGCCAA GCGCGCAATT AACCCTCACT
            181 AAAGGGAACA AAAGCTGGAG CTCATCGATA CTCGACATTG ATTATTGACT AGTTATTAAT
            241 AGTAATCAAT TACGGGGTCA TTAGTTCATA GCCCATATAT GGAGTTCCGC GTTACATAAC
            301 TTACGGTAAA TGGCCCGCCT GGCTGACCGC CCAACGACCC CCGCCCATTG ACGTCAATAA
            361 TGACGTATGT TCCCATAGTA ACGCCAATAG GGACTTTCCA TTGACGTCAA TGGGTGGAGT
            421 ATTTACGGTA AACTGCCCAC TTGGCAGTAC ATCAAGTGTA TCATATGCCA AGTACGCCCC
            481 CTATTGACGT CAATGACGGT AAATGGCCCG CCTGGCATTA TGCCCAGTAC ATGACCTTAT
            541 GGGACTTTCC TACTTGGCAG TACATCTACG TATTAGTCAT CGCTATTACC ATGGTCGAGG
            601 TGAGCCCCAC GTTCTGCTTC ACTCTCCCCA TCTCCCCCCC CTCCCCACCC CCAATTTTGT
            661 ATTTATTTAT TTTTTAATTA TTTTGTGCAG CGATGGGGGC NNNGGGGGGG GNNNNNNNNN
          721 N
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Abbasi, F., RSPH6A is required for sperm flagellum formation and male fertility in mice. J. Cell Sci. 131 (19). pii: jcs221648 (2018). PMID 30185526.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl