Expression vector of mouse Rsph1.
Alternative name | mouse Rsph1 on PA tagged pCAG vector |
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Clone info. | Expression vector of mouse radial spoke head 1 homolog (Rsph1) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal. |
Comment | Rsph1 was amplified from mouse testis cDNA. The PA tag is a dodecapeptide (GVAMPGAEDDVV) that is recognized by this high-affinity antibody. Expression was confirmed by the depositor with western blotting. |
Vector backbone | pCAGGS (plasmid) |
Size of vector backbone | 4.8 kb |
Selectable markers | Ampicillin |
Gene/insert name | mouse Rsph1 cDNA |
Depositor|Developer | Ikawa, Masahito | Miyata, haruhiko | |
Sequence (full) ![]() |
RDB17449zzk02.seq provided by the depositor |
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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Abbasi, F et al. J. Cell Sci. 131(19) jcs221648, 2018) |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Abbasi, F et al. J. Cell Sci. 131(19) jcs221648, 2018)。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB17449 | pCAG_mRsph1-PA | DNA solution |
Materials & Methods section:
The pCAG_mRsph1-PA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17449). |
Reference section:
Abbasi, F., Miyata, H., Shimada, K., Morohoshi, A., Nozawa, K., Matsumura, T., Xu, Z., Pratiwi, P., Ikawa, M., RSPH6A is required for sperm flagellum formation and male fertility in mice. J. Cell Sci. 131 (19). pii: jcs221648 (2018). PMID 30185526. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB17449_A9G9p1-1.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: pAxCA_F1 (Pr0164) Region: CAG pro, insert 5' Sequence file: RDB17449_A9G9a.seq ![]() |
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>D06337A1_A9Gg_2_pAxCA_F1_A01_01_ABI24.ab1 1 NNNNNNNNNN NNNNNNNGCC TTCTTCTTTT TCCNNCNGCT CCTGGGCAAC GTGCTGGTTA 61 TTGTGCTGTC TCATCATTTT GGCAAATCTA GAATCGATGC GGCCGCAGTC GACAAGCTTG 121 CCGCCATGTC GGACCTGGGC TCTGAGGAGT TAGAGGAGGA GGGAGAGAAC GACCTTGGGG 181 AGTACGAAGG GGAGCGCAAT GAGGTGGGAG AACGACACGG ACATGGGAAA GCACGACTGC 241 CCAACGGGGA CACATATGAA GGAAGCTATG AGTTTGGAAA AAGACATGGC CAGGGGACTT 301 ACAAATTTAA GAATGGGGCC CGGTACACCG GAGACTATGT CAAAAATAAA AAGCACGGCC 361 AAGGCACCTT TATCTATCCA GATGGATCCA GATATGAAGG GGAGTGGGCT GATGACCAGA 421 GGCACGGCCA AGGCGTGTAC TACTATGTCA ACAATGACAC CTACACAGGG GAGTGGTTCA 481 ATCATCAAAG GCACGGGCAA GGCACCTACC TCTACGCAGA GACCGGCAGT AAGTATGTCG 541 GTACCTGGGT GCACGGACAG CAGGAGGGTG CTGCCGAGCT CATCCACCTA AACCACAGGT 601 ACCAGGGCAA GTTCATGAAC AAAAATCCTG TGGGCCCTGG AAAGTACGTA TTTGATATTG 661 GATGCGAACA GCACGGTGAA TATCGCCTCA CAGATACGGA AAGAGGAGAA GAGGAGGANG 721 AGGAAGAGAC ATTAGTGAAC ATCGTTCCNA AATGGAAAGC TCTCAACATC ACAGAANTGG 781 CCCTGTGGAC TCCAACCCTC TCCGANGANC AGCCTCCCCC TGANGGCCNA NGCCAGGANG 841 AACCGCANGG NCTTACTGGC GTNNNTGANC CCTCNNANNA NATCCAGGCA GAANNNNTTT 901 GANGGCGANC TGNANCCNAN GGGGGGCCGA NGAAGANGNA NANNCGTNCA GGNNNNANAN 961 CCAGGANNAC AGCTACNANN TNNANNANGG NANCTNNNAN NTTNNNNNNN NNN // |
Primer: pCA-bgter (Pr0066) Region: PA tag, insert 3' Sequence file: RDB17449_A9G9b.seq ![]() |
>D06337A1_A9Gg_2_pCA-bgter_B01_04_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNTT TGNNNNANGN NNNNNNCTCN GTGGTATTTG TGAGCCAGGG 61 CATTGGCCAC ACCAGCCACC ACCTTCTGAT AGGCAGCCTG CACCTGAGGA GTCTCGAGCT 121 AGGCAGGCGC CACTTGGCTG GTCTCTGTGC CCCCGCCCCC GATCACCACA TCATCTTCGG 181 CACCTGGCAT GGCAACGCCG ATATCGAATT CATCCTGGAG GTCTGACGGT TCTTCATCAA 241 AGTTCAAGTT TCCCTGGTCT ATGTCGTAGC TGTTCTCCTG GCTCTCCTGC CTGAACGTGT 301 CTACGTCTTC ATCGGCCCCC CTCGGCTCCA GCTCGCCCTC AAAACCTTCT GCCTGGATGT 361 CTTCTGAGGG GTCACCCACG CCAGTAAGTC CCTGCGGTTC CTCCTGGCCT TGGCCCTCAG 421 GGGGAGGCTG CTCCTCGGAG AGGGTTGGAG TCCACAGGGC CAATTCTGTG ATGTTGAGAG 481 CTTTCCATTT TGGAACGATG TTCACTAATG TCTCTTCCTC CTCCTCCTCT TCTCCTCTTT 541 CCGTATCTGT GAGGCGATAT TCACCGTGCT GTTCGCATCN AATATCAAAT ACGTACTTTC 601 CAGGGCCCAC AGGANTTTTG TTCATGAACT TGCCCTGGTA CCTGTGGTTT ANGTGGANGA 661 GCTCGGCAGC ACCCTCCTGC TGTCCGTGCA CNCAGGTACC GACATACTTA CTGCCGGTCT 721 CTGCGTANAG GTANGTGCCT TGNCCGNGCC TTTGANGANT NNNNNNCTCC CCTGNGTNNN 781 TGTCATNGTT GANNTAGNAN TACACGCCTN GGCCGNGCNN CTNGTCATCA NCCCNNTNCC 841 NTNNNTATCN NNNNNNNTCN NNNNANNNAA NNNNNNNNNG NNNNNNNTTT TNANTTTNGA 901 NNNANNCTNN NNNGNNNNNG GNNCNNNNNN NNNANNTNNN NNNNNCNNNN NNNNNN // |
Primer: pUC-CAPbs_up_F (Pr0536) Region: lac pro, lacO, T3 pro, CMV en, CAG pro Sequence file: RDB17449_A9G9c.seq ![]() |
>D06337A1_A9Gg_2_pUC-CAPbs_up_F_C01_07_ABI24.ab1 1 NNNNNNNNNN NNNNNANNNN NNNNNNNCAN TTAANGTGAG TTAGCTCNCT NNTTAGGCAC 61 CCCAGGCTTT ACACTNNNNN CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC 121 AATTTCACAC AGGAAACAGC TATGACCATG ATTACGCCAA GCGCGCAATT AACCCTCACT 181 AAAGGGAACA AAAGCTGGAG CTCATCGATA CTCGACATTG ATTATTGACT AGTTATTAAT 241 AGTAATCAAT TACGGGGTCA TTAGTTCATA GCCCATATAT GGAGTTCCGC GTTACATAAC 301 TTACGGTAAA TGGCCCGCCT GGCTGACCGC CCAACGACCC CCGCCCATTG ACGTCAATAA 361 TGACGTATGT TCCCATAGTA ACGCCAATAG GGACTTTCCA TTGACGTCAA TGGGTGGAGT 421 ATTTACGGTA AACTGCCCAC TTGGCAGTAC ATCAAGTGTA TCATATGCCA AGTACGCCCC 481 CTATTGACGT CAATGACGGT AAATGGCCCG CCTGGCATTA TGCCCAGTAC ATGACCTTAT 541 GGGACTTTCC TACTTGGCAG TACATCTACG TATTAGTCAT CGCTATTACC ATGGTCGAGG 601 TGAGCCCCAC GTTCTGCTTC ACTCTCCCCA TCTCCCCCCC CTCCCCACCC CCAATTTTGT 661 ATTTATTTAT TTTTTAATTA TTTTGTGCAG CGATGGGGGC NNNGGGGGGG GNNNNNNNNN 721 N // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Abbasi, F., RSPH6A is required for sperm flagellum formation and male fertility in mice. J. Cell Sci. 131 (19). pii: jcs221648 (2018). PMID 30185526. [link to RRC of NBRP] |
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