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pCAG_mRsph1-PA

Expression vector of mouse Rsph1.

Catalog number RDB17449
Resource name pCAG_mRsph1-PA
Alternative name mouse Rsph1 on PA tagged pCAG vector
Clone info. Expression vector of mouse radial spoke head 1 homolog (Rsph1) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal.
Rsph1 was amplified from mouse testis cDNA. The PA tag is a dodecapeptide (GVAMPGAEDDVV) that is recognized by this high-affinity antibody. Expression was confirmed by the depositor with western blotting.
Vector backbone pCAGGS (plasmid)
Size of vector backbone 4.8 kb
Selectable markers Ampicillin
Gene/insert name mouse Rsph1 cDNA
Depositor Ikawa, Masahito | Miyata, Haruhiko |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Abbasi, F et al. J. Cell Sci. 131(19) jcs221648, 2018)
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB17449 pCAG_mRsph1-PA Under QC test. Please contact us. DNA solution

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References and tips

Electronic file

Original reference

original Abbasi, F., RSPH6A is required for sperm flagellum formation and male fertility in mice. J. Cell Sci. 131 (19). pii: jcs221648 (2018). PMID 30185526.

Further references such as user reports and related articles (go to bottom)

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Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

original Abbasi, F., RSPH6A is required for sperm flagellum formation and male fertility in mice. J. Cell Sci. 131 (19). pii: jcs221648 (2018). PMID 30185526.

2019.11.05

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