Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCAG_mRsph1-PA

Expression vector of mouse Rsph1.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Abbasi, F et al. J. Cell Sci. 131(19) jcs221648, 2018)
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Abbasi, F et al. J. Cell Sci. 131(19) jcs221648, 2018)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17449_A9G9p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 Region: CAG pro, insert 5' Sequence file: RDB17449_A9G9a.seq
>D06337A1_A9Gg_2_pAxCA_F1_A01_01_ABI24.ab1     1 NNNNNNNNNN NNNNNNNGCC TTCTTCTTTT TCCNNCNGCT CCTGGGCAAC GTGCTGGTTA    61 TTGTGCTGTC TCATCATTTT GGCAAATCTA GAATCGATGC GGCCGCAGTC GACAAGCTTG   121 CCGCCATGTC GGACCTGGGC TCTGAGGAGT TAGAGGAGGA GGGAGAGAAC GACCTTGGGG   181 AGTACGAAGG GGAGCGCAAT GAGGTGGGAG AACGACACGG ACATGGGAAA GCACGACTGC   241 CCAACGGGGA CACATATGAA GGAAGCTATG AGTTTGGAAA AAGACATGGC CAGGGGACTT   301 ACAAATTTAA GAATGGGGCC CGGTACACCG GAGACTATGT CAAAAATAAA AAGCACGGCC   361 AAGGCACCTT TATCTATCCA GATGGATCCA GATATGAAGG GGAGTGGGCT GATGACCAGA   421 GGCACGGCCA AGGCGTGTAC TACTATGTCA ACAATGACAC CTACACAGGG GAGTGGTTCA   481 ATCATCAAAG GCACGGGCAA GGCACCTACC TCTACGCAGA GACCGGCAGT AAGTATGTCG   541 GTACCTGGGT GCACGGACAG CAGGAGGGTG CTGCCGAGCT CATCCACCTA AACCACAGGT   601 ACCAGGGCAA GTTCATGAAC AAAAATCCTG TGGGCCCTGG AAAGTACGTA TTTGATATTG   661 GATGCGAACA GCACGGTGAA TATCGCCTCA CAGATACGGA AAGAGGAGAA GAGGAGGANG   721 AGGAAGAGAC ATTAGTGAAC ATCGTTCCNA AATGGAAAGC TCTCAACATC ACAGAANTGG   781 CCCTGTGGAC TCCAACCCTC TCCGANGANC AGCCTCCCCC TGANGGCCNA NGCCAGGANG   841 AACCGCANGG NCTTACTGGC GTNNNTGANC CCTCNNANNA NATCCAGGCA GAANNNNTTT   901 GANGGCGANC TGNANCCNAN GGGGGGCCGA NGAAGANGNA NANNCGTNCA GGNNNNANAN 961 CCAGGANNAC AGCTACNANN TNNANNANGG NANCTNNNAN NTTNNNNNNN NNN //
Primer: pCA-bgter Region: PA tag, insert 3' Sequence file: RDB17449_A9G9b.seq
>D06337A1_A9Gg_2_pCA-bgter_B01_04_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNTT TGNNNNANGN NNNNNNCTCN GTGGTATTTG TGAGCCAGGG    61 CATTGGCCAC ACCAGCCACC ACCTTCTGAT AGGCAGCCTG CACCTGAGGA GTCTCGAGCT   121 AGGCAGGCGC CACTTGGCTG GTCTCTGTGC CCCCGCCCCC GATCACCACA TCATCTTCGG   181 CACCTGGCAT GGCAACGCCG ATATCGAATT CATCCTGGAG GTCTGACGGT TCTTCATCAA   241 AGTTCAAGTT TCCCTGGTCT ATGTCGTAGC TGTTCTCCTG GCTCTCCTGC CTGAACGTGT   301 CTACGTCTTC ATCGGCCCCC CTCGGCTCCA GCTCGCCCTC AAAACCTTCT GCCTGGATGT   361 CTTCTGAGGG GTCACCCACG CCAGTAAGTC CCTGCGGTTC CTCCTGGCCT TGGCCCTCAG   421 GGGGAGGCTG CTCCTCGGAG AGGGTTGGAG TCCACAGGGC CAATTCTGTG ATGTTGAGAG   481 CTTTCCATTT TGGAACGATG TTCACTAATG TCTCTTCCTC CTCCTCCTCT TCTCCTCTTT   541 CCGTATCTGT GAGGCGATAT TCACCGTGCT GTTCGCATCN AATATCAAAT ACGTACTTTC   601 CAGGGCCCAC AGGANTTTTG TTCATGAACT TGCCCTGGTA CCTGTGGTTT ANGTGGANGA   661 GCTCGGCAGC ACCCTCCTGC TGTCCGTGCA CNCAGGTACC GACATACTTA CTGCCGGTCT   721 CTGCGTANAG GTANGTGCCT TGNCCGNGCC TTTGANGANT NNNNNNCTCC CCTGNGTNNN   781 TGTCATNGTT GANNTAGNAN TACACGCCTN GGCCGNGCNN CTNGTCATCA NCCCNNTNCC   841 NTNNNTATCN NNNNNNNTCN NNNNANNNAA NNNNNNNNNG NNNNNNNTTT TNANTTTNGA 901 NNNANNCTNN NNNGNNNNNG GNNCNNNNNN NNNANNTNNN NNNNNCNNNN NNNNNN //
Primer: pUC-CAPbs_up_F Region: lac pro, lacO, T3 pro, CMV en, CAG pro Sequence file: RDB17449_A9G9c.seq
>D06337A1_A9Gg_2_pUC-CAPbs_up_F_C01_07_ABI24.ab1     1 NNNNNNNNNN NNNNNANNNN NNNNNNNCAN TTAANGTGAG TTAGCTCNCT NNTTAGGCAC    61 CCCAGGCTTT ACACTNNNNN CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC   121 AATTTCACAC AGGAAACAGC TATGACCATG ATTACGCCAA GCGCGCAATT AACCCTCACT   181 AAAGGGAACA AAAGCTGGAG CTCATCGATA CTCGACATTG ATTATTGACT AGTTATTAAT   241 AGTAATCAAT TACGGGGTCA TTAGTTCATA GCCCATATAT GGAGTTCCGC GTTACATAAC   301 TTACGGTAAA TGGCCCGCCT GGCTGACCGC CCAACGACCC CCGCCCATTG ACGTCAATAA   361 TGACGTATGT TCCCATAGTA ACGCCAATAG GGACTTTCCA TTGACGTCAA TGGGTGGAGT   421 ATTTACGGTA AACTGCCCAC TTGGCAGTAC ATCAAGTGTA TCATATGCCA AGTACGCCCC   481 CTATTGACGT CAATGACGGT AAATGGCCCG CCTGGCATTA TGCCCAGTAC ATGACCTTAT   541 GGGACTTTCC TACTTGGCAG TACATCTACG TATTAGTCAT CGCTATTACC ATGGTCGAGG   601 TGAGCCCCAC GTTCTGCTTC ACTCTCCCCA TCTCCCCCCC CTCCCCACCC CCAATTTTGT   661 ATTTATTTAT TTTTTAATTA TTTTGTGCAG CGATGGGGGC NNNGGGGGGG GNNNNNNNNN 721 N //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl