Resource data sheet
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pCAG-MCS-BioID2-3xFLAG

CAG promoter-driven expression of BioID2-3xFLAG-fused protein (C terminus).

Catalog number RDB19659
Resource name pCAG-MCS-BioID2-3xFLAG
Clone info. CAG promoter-driven expression of BioID2-3xFLAG-fused protein (C terminus). BioID2 is a proximity labeling tool for proteomic analysis and is derived from the hyperthermophilic bacterium A. aeolicus.
Vector backbone pCAG1.1 (plasmid)
Size of vector backbone 5.2 kb
Selectable markers Ampicillin
Gene/insert name Aquifex aeolicus biotin protein ligase (BioID2); synthetic FLAG-tag.
Depositor|Developer Ikawa, Masahito | Oura, Seiya |
 
Sequence (full) RDB19659hts01.seqcheck assembled from experimentally sequenced data
Test sheet RDB19659_B2Gjp1-1.pdf 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Oura, S. et al. Sci. Rep. 12 (1): 22198 (2022))
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提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Oura, S. et al. Sci. Rep. 12 (1): 22198 (2022))。
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19659 pCAG-MCS-BioID2-3xFLAG DNA solution

How to cite this biological resource

Materials & Methods section:

The pCAG-MCS-BioID2-3xFLAG was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19659).

Reference section:

Oura, S., Ninomiya, A., Sugihara, F., Matzuk, M.M., Ikawa, M., Proximity-dependent biotin labeling in testicular germ cells identified TESMIN-associated proteins. Sci. Rep. 12 (1): 22198 (2022). PMID 36564444. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Oura, S., Proximity-dependent biotin labeling in testicular germ cells identified TESMIN-associated proteins. Sci. Rep. 12 (1): 22198 (2022). PMID 36564444. [link to RRC of NBRP]