Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pClgn1.1_Kctd19-3xHA (#RDB19248)

Expression vector of mouse Kctd19 tagged with 3xHA at C-terminus.

Clone info. Expression vector of mouse potassium channel tetramerization domain containing 19 (Kctd19) tagged with 3xHA at C-terminus. See Fig.4 of Oura, S. et al. PLoS Genet. 17 (5): e1009412, 2021.
Comment Expression was confirmed by the depositor with immunoblotting.
Vector backbone pClgn1.1 (plasmid)
Selectable markers Ampicillin
Gene/insert name mouse Kctd19 cDNA
Depositor|Developer Ikawa, Masahito | Oura, Seiya |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Oura, S. et al., PLoS Genet., 17 (5): e1009412, 2021). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Oura, S. et al., PLoS Genet., 17 (5): e1009412, 2021)。利用者が非営利目的の教育・研究以外の目的に用いる場合は、大阪大学と別途MTAを締結すること。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB19248 pClgn1.1_Kctd19-3xHA Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pClgn1.1_Kctd19-3xHA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19248).

Reference section:

Oura, S., Koyano, T., Kodera, C., Horisawa-Takada, Y., Matsuyama, M., Ishiguro, K.I., Ikawa, M., KCTD19 and its associated protein ZFP541 are independently essential for meiosis in male mice. PLoS Genet. 17 (5): e1009412 (2021). PMID 33961623. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Oura, S., KCTD19 and its associated protein ZFP541 are independently essential for meiosis in male mice. PLoS Genet. 17 (5): e1009412 (2021). PMID 33961623. [link to RRC of NBRP]