Resource data sheet
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pCAG-mDrc3-PA (#RDB19240)

Expression vector of mouse Drc3 tagged with PA.

Clone info. Expression vector of mouse dynein regulatory complex subunit 3 (Drc3) tagged with PA at C-terminus.
Comment Expression was confirmed by the depositor with Western blotting and immunoprecipitation.
Vector backbone pCAGGS (plasmid)
Size of vector backbone 5.2 kb
Selectable markers Ampicillin
Gene/insert name mouse Drc3 cDNA
Depositor|Developer Ikawa, Masahito |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Morohoshi, A. et al., PLoS Genet., 16 (1): e1008585, 2020). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Morohoshi, A. et al., PLoS Genet., 16 (1): e1008585, 2020)。利用者が非営利目的の教育・研究以外の目的に用いる場合は、大阪大学と別途MTAを締結すること。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB19240 pCAG-mDrc3-PA Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pCAG-mDrc3-PA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19240).

Reference section:

Morohoshi, A., Miyata, H., Shimada, K., Nozawa, K., Matsumura, T., Yanase, R., Shiba, K., Inaba, K., Ikawa, M., Nexin-Dynein regulatory complex component DRC7 but not FBXL13 is required for sperm flagellum formation and male fertility in mice. PLoS Genet. 16 (1): e1008585 (2020). PMID 31961863. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Morohoshi, A., Nexin-Dynein regulatory complex component DRC7 but not FBXL13 is required for sperm flagellum formation and male fertility in mice. PLoS Genet. 16 (1): e1008585 (2020). PMID 31961863. [link to RRC of NBRP]