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pPB-Prm1 pro-H3.3-mCherry (#RDB20978)

Piggybac expression vector of histone H3.3-mCherry fusion protein (H33C) under the control of protamine 1 (Prm1) promoter.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Makino, Y., Front. Cell Dev. Biol. 2: 30 (2014). PMID 25364737. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Piggybac expression vector of histone H3.3-mCherry fusion protein (H33C) under the control of protamine 1 (Prm1) promoter. See Fig. 1 of Makino. Y, et al., Front. Cell Dev. Biol., 2:30, 2014. doi: 10.3389/fcell.2014.00030.
Vector backbone pPBbsr2 (plasmid)
Selectable markers Ampicillin (E. coli)
Gene/insert name mouse Prm1 gene promoter; human histone H3.3 cDNA; Discosoma sp. fluorescent protein (mCcherry) cDNA.
Depositor|Developer Ikawa, Masahito | Okada, Yuki | Makino, Yoshinori |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Yoshinori Makino et al., Front Cell Dev Biol. 23; 2:30, 2014) The RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with The University of Osaka(mta-egr@biken.osaka-u.ac.jp).
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piggyBac®トランスポゾンを内包するバイオリソースです。Limited Use License Agreement (LULA) [Word]の提出をお願いします。
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yoshinori Makino et al., Front Cell Dev Biol. 23; 2:30, 2014)。利用者が非営利目的の教育・研究以外の目的に用いる場合は、大阪大学(mta-egr@biken.osaka-u.ac.jp)と別途MTAを締結すること。

Catalog # Resource name Shipping form Fee
RDB20978 pPB-Prm1 pro-H3.3-mCherry DNA solution

How to cite this biological resource

Materials & Methods section:

The pPB-Prm1 pro-H3.3-mCherry was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20978).

Reference section:

Makino, Y., Inoue, E., Hada, M., Aoshima, K., Kitano, S., Miyachi, H., Okada, Y., Generation of a dual-color reporter mouse line to monitor spermatogenesis in vivo. Front. Cell Dev. Biol. 2: 30 (2014). PMID 25364737. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Makino, Y., Generation of a dual-color reporter mouse line to monitor spermatogenesis in vivo. Front. Cell Dev. Biol. 2: 30 (2014). PMID 25364737. [PubMed] [Article] [RRC of NBRP]