Gene Engineering Division >  Depositors List > 

Norihiko Misawa's Resource

Norihiko Misawa's web page in English /  in Japanese.

Learn more about their research, in English  /  in Japanese (by Reseachmap[link])

Catalog #Name of cloneRunning titlePublication
RDB19483pACCRT-EBpACYC184 vector-based plasmid for biosynthesis of phytoene in E. coli (crtE, crtB).Misawa, N., Structure and functional analysis of a marine bacterial carotenoid biosynthesis gene cluster and astaxanthin biosynthetic pathway proposed at the gene level. J. Bacteriol. 177 (22): 6575-6584 (1995). PMID 7592436. (original). [link to RRC of NBRP]
RDB19484pACCRT-EIBpACYC184 vector-based plasmid for biosynthesis of lycopene in E. coli (crtE, crtB, crtI).Misawa, N., Structure and functional analysis of a marine bacterial carotenoid biosynthesis gene cluster and astaxanthin biosynthetic pathway proposed at the gene level. J. Bacteriol. 177 (22): 6575-6584 (1995). PMID 7592436. (original). [link to RRC of NBRP]
RDB19485pACCRT-EB-RcIpACYC184 vector-based plasmid for biosynthesis of neurosporene in E. coli (crtE, crtB, Rc-crtI).Takaichi, S., The carotenoid 7,8-dihydro-psi end group can be cyclized by the lycopene cyclases from the bacterium Erwinia uredovora and the higher plant Capsicum annuum. Eur. J. Biochem. 241 (1): 291-296 (1996). PMID 8898919. (original). [link to RRC of NBRP]
RDB19486pACCAR16 delta crtXpACYC184 vector-based plasmid for biosynthesis of β-carotene in E. coli (crtE, crtB, crtI, crtY).Misawa, N., Structure and functional analysis of a marine bacterial carotenoid biosynthesis gene cluster and astaxanthin biosynthetic pathway proposed at the gene level. J. Bacteriol. 177 (22): 6575-6584 (1995). PMID 7592436. (original). [link to RRC of NBRP]
RDB19487pACCAR25 delta crtXpACYC184 vector-based plasmid for biosynthesis of zeaxanthin in E. coli (crtE, crtB, crtI, crtY, crtZ).Misawa, N., Structure and functional analysis of a marine bacterial carotenoid biosynthesis gene cluster and astaxanthin biosynthetic pathway proposed at the gene level. J. Bacteriol. 177 (22): 6575-6584 (1995). PMID 7592436. (original). [link to RRC of NBRP]
RDB19488pAC-CanthapACYC184 vector-based plasmid for biosynthesis of canthaxanthin in E. coli (crtE, crtB, crtI, crtY, crtW).Nishida, Y., Elucidation of a carotenoid biosynthesis gene cluster encoding a novel enzyme, 2,2'-beta-hydroxylase, from Brevundimonas sp. strain SD212 and combinatorial biosynthesis of new or rare xanthophylls. Appl. Environ. Microbiol. 71 (8): 4286-4296 (2005). PMID 16085816. (original). [link to RRC of NBRP]
RDB19489pAC-AstapACYC184 vector-based plasmid for biosynthesis of astaxanthin in E. coli (crtE, crtB, crtI, crtY, crtZ, crtW).Nishida, Y., Elucidation of a carotenoid biosynthesis gene cluster encoding a novel enzyme, 2,2'-beta-hydroxylase, from Brevundimonas sp. strain SD212 and combinatorial biosynthesis of new or rare xanthophylls. Appl. Environ. Microbiol. 71 (8): 4286-4296 (2005). PMID 16085816. (original). [link to RRC of NBRP]
RDB19490pRK-idiExpression plasmid of the isopentenyl diphoshate (IPP) isomerase gene (IDI), which is used to incerease carotenoid production with another carotenogenic plasmid in E. coli (XdIDI).Albrecht, M., Metabolic engineering of the terpenoid biosynthetic pathway of Escherichia coli for production of the carotenoids β-carotene and zeaxanthin. Biotechnology Letters 21: 791–795 (1999). (original). [link to RRC of NBRP]
RDB19491pACHP-PhypACYC184 vector-based plasmid for biosynthesis of phytoene (addtionally including the HpIDI gene) in E. coli. plasmid pACCRT-EB with HpIDI (HpIDI, crtE, crtB).Takemura, M., Elucidation of the whole carotenoid biosynthetic pathway of aphids at the gene level and arthropodal food chain involving aphids and the red dragonfly. BMC Zoology 6:19 (2021). (original). [link to RRC of NBRP]
RDB19492pACHP-LycpACYC184 vector-based plasmid for biosynthesis of lycopene (addtionally including the HpIDI gene) in E. coli. Plasmid pACCRT-EIB with HpIDI (HpIDI, crtE, crtB, crtI).Takemura, M., Elucidation of the whole carotenoid biosynthetic pathway of aphids at the gene level and arthropodal food chain involving aphids and the red dragonfly. BMC Zoology 6:19 (2021). (original). [link to RRC of NBRP]
RDB19493pACHP-BetapACYC184 vector-based plasmid for biosynthesis of β-carotene (addtionally including the HpIDI gene) in E. coli. plasmid pACCAR16ΔcrtX with HpIDI (HpIDI, crtE, crtB, crtI, crtY).Takemura, M., Biosynthetic routes of hydroxylated carotenoids (xanthophylls) in Marchantia polymorpha, and production of novel and rare xanthophylls through pathway engineering in Escherichia coli. Planta 241 (3): 699-710 (2015). PMID 25467956. (original). [link to RRC of NBRP]
RDB19494pACHP-ZeapACYC184 vector-based plasmid for biosynthesis of zeaxanthin (addtionally including the HpIDI gene) in E. coli. plasmid pACCAR25ΔcrtX with HpIDI (HpIDI, crtE, crtB, crtI, crtY, crtZ).Takemura, M., (6E) and (6Z)-9′-Aporhodoxanthinone, novel carotenoids produced in zeaxanthin-synthesizing-Escherichia coli by redox stress. Tetrahedron Letters 56(44): 6063-6065 (2015). (original). [link to RRC of NBRP]
RDB19495pAC-HIEBpACYC184 vector-based plasmid for biosynthesis of phytoene (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB).Misawa, N., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. (original). [link to RRC of NBRP]
RDB19496pAC-HIEBIpACYC184 vector-based plasmid for biosynthesis of lycopene (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI).Takemura, M., Elucidation of the whole carotenoid biosynthetic pathway of aphids at the gene level and arthropodal food chain involving aphids and the red dragonfly. BMC Zoology 6:19 (2021). (original). [link to RRC of NBRP]
RDB19497pAC-HIEBIApACYC184 vector-based plasmid for biosynthesis of γ-carotene (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, cruA).Takemura, M., Pathway engineering for high-yield production of lutein in Escherichia coli. Synth. Biol. (Oxf) 6 (1): ysab012 (2021). PMID 34712837. (original). [link to RRC of NBRP]
RDB19498pAC-HIEBIYpACYC184 vector-based plasmid for biosynthesis of β-carotene (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, crtY).Misawa, N., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. (original). [link to RRC of NBRP]
RDB19499pAC-HIEBIYZpACYC184 vector-based plasmid for biosynthesis of zeaxanthin (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, crtY, crtZ).Misawa, N., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. (original). [link to RRC of NBRP]
RDB19500pAC-HIEBIYZWpACYC184 vector-based plasmid for biosynthesis of astaxanthin (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, crtY, crtZ, crtW).Misawa, N., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. (original). [link to RRC of NBRP]
RDB19501pAC-HIEBIYZGpACYC184 vector-based plasmid for biosynthesis of nostoxanthin (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, crtY, crtZ, crtG).Misawa, N., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. (original). [link to RRC of NBRP]
RDB19502pAC-HIEBI-ApCrtYB3pACYC184 vector-based plasmid for biosynthesis of β,γ-carotene (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, ApCrtYB3).Takemura, M., Elucidation of the whole carotenoid biosynthetic pathway of aphids at the gene level and arthropodal food chain involving aphids and the red dragonfly. BMC Zoology 6:19 (2021). (original). [link to RRC of NBRP]
RDB19503pAC-HIEBI-ApCrtYB1pACYC184 vector-based plasmid for biosynthesis of torulene (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, ApCrtYB1).Takemura, M., Elucidation of the whole carotenoid biosynthetic pathway of aphids at the gene level and arthropodal food chain involving aphids and the red dragonfly. BMC Zoology 6:19 (2021). (original). [link to RRC of NBRP]
RDB19504pAC-HIMpACYC184 vector-based plasmid for biosynthesis of 15-cis-4,4'-diapophytoene (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtM).Takemura, M., Heterologous production of novel and rare C30-carotenoids using Planococcus carotenoid biosynthesis genes. Microb. Cell Fact. 20 (1): 194 (2021). PMID 34627253. (original). [link to RRC of NBRP]
RDB19505pAC-HIMNpACYC184 vector-based plasmid for biosynthesis of 4,4'-diaponeurosporene (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtM, crtN).Takemura, M., Heterologous production of novel and rare C30-carotenoids using Planococcus carotenoid biosynthesis genes. Microb. Cell Fact. 20 (1): 194 (2021). PMID 34627253. (original). [link to RRC of NBRP]
RDB19506pRK-HIEBpRK404 vector-based plasmid for biosynthesis of phytoene (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB).Misawa, N., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. (original). [link to RRC of NBRP]
RDB19507pRK-HIEBIpRK404 vector-based plasmid for biosynthesis of lycopene (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI).Misawa, N., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. (original). [link to RRC of NBRP]
RDB19508pRK-HIEBIYpRK404 vector-based plasmid for biosynthesis of β-carotene (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, crtY).Misawa, N., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. (original). [link to RRC of NBRP]
RDB19509pRK-HIEBIYZpRK404 vector-based plasmid for biosynthesis of zeaxanthin (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, crtY, crtZ).Misawa, N., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. (original). [link to RRC of NBRP]
RDB19510pRK-HIEBIYZWpRK404 vector-based plasmid for biosynthesis of astaxanthin (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, crtY, crtZ, crtW).Misawa, N., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. (original). [link to RRC of NBRP]
RDB19511pRK-HIEBIYZGpRK404 vector-based plasmid for biosynthesis of nostoxanthin (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, crtY, crtZ, crtG).Misawa, N., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. (original). [link to RRC of NBRP]
RDB19512pRK-HIEBI-MpLCYb delta TP-MpLCYe-Z-EpgpRK404 vector-based plasmid for biosynthesis of zeinoxanthin (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, MpLCYb, MpLCYe, crtZ, Pag-crtE).Takemura, M., Pathway engineering for high-yield production of lutein in Escherichia coli. Synth. Biol. (Oxf) 6 (1): ysab012 (2021). PMID 34712837. (original). [link to RRC of NBRP]
RDB19513pRK-HIEBIYZ-FhCCD2pRK404 vector-based plasmid for biosynthesis of crocetindial (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, crtY, crtZ, FhCCD2).Misawa, N., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. (original). [link to RRC of NBRP]
RDB19514pRK-HIEBIYZ-FhCCD2-NcALD8pRK404 vector-based plasmid for biosynthesis of crocetin (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, crtY, crtZ, FhCCD2, NcALD8).Misawa, N., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. (original). [link to RRC of NBRP]
RDB19515pUCBre-WcrtW-expression plasmid for production of astaxanthin and other ketocarotenoids when coexpressed with a plasmid to biosynthesize zeaxanthin in E. coli.Nishida, Y., Elucidation of a carotenoid biosynthesis gene cluster encoding a novel enzyme, 2,2'-beta-hydroxylase, from Brevundimonas sp. strain SD212 and combinatorial biosynthesis of new or rare xanthophylls. Appl. Environ. Microbiol. 71 (8): 4286-4296 (2005). PMID 16085816. (original). [link to RRC of NBRP]
RDB19516pUCBre-O11crtG-expression plasmid for production of nostoxanthin and caloxanthin when coexpressed with a plasmid to biosynthesize zeaxanthin in E. coli.Nishida, Y., Elucidation of a carotenoid biosynthesis gene cluster encoding a novel enzyme, 2,2'-beta-hydroxylase, from Brevundimonas sp. strain SD212 and combinatorial biosynthesis of new or rare xanthophylls. Appl. Environ. Microbiol. 71 (8): 4286-4296 (2005). PMID 16085816. (original). [link to RRC of NBRP]
RDB19517pUC-ApTorApTOR-expression plasmid for production of 3,4-didehydrolycopene and lycopene when coexpressed with a plasmid to biosynthesize phytoene in E. coli.Takemura, M., Elucidation of the whole carotenoid biosynthetic pathway of aphids at the gene level and arthropodal food chain involving aphids and the red dragonfly. BMC Zoology 6:19 (2021). (original). [link to RRC of NBRP]
RDB19518pUC-CaZEPCaZEP-expression plasmid for production of violaxanthin and antheraxanthin when coexpressed with a plasmid to biosynthesize zeaxanthin in E. coli.Takemura, M., Pathway engineering for efficient biosynthesis of violaxanthin in Escherichia coli. Appl. Microbiol. Biotechnol. 103 (23-24): 9393-9399 (2019). PMID 31673744. (original). [link to RRC of NBRP]
RDB19519pETDuet-MpLCYb/MpLCYe MpLCYb- and MpLCYe-expression plasmid for production of α-carotene (and β-carotene) when coexpressed with a plasmid to biosynthesize lycopene in E. coli.Takemura, M., Biosynthetic routes of hydroxylated carotenoids (xanthophylls) in Marchantia polymorpha, and production of novel and rare xanthophylls through pathway engineering in Escherichia coli. Planta 241 (3): 699-710 (2015). PMID 25467956. (original). [link to RRC of NBRP]
RDB19520pRSFDuet-MpBHYMpBHY-expression plasmid for production of zeinoxanthin (and zeaxanthin) when coexpressed with a plasmid to biosynthesize α-carotene (and β-carotene) in E. coli.Takemura, M., Biosynthetic routes of hydroxylated carotenoids (xanthophylls) in Marchantia polymorpha, and production of novel and rare xanthophylls through pathway engineering in Escherichia coli. Planta 241 (3): 699-710 (2015). PMID 25467956. (original). [link to RRC of NBRP]
RDB19521pRSFDuet-MpBHY/MpCYP97CMpBHY- and MpCYP97C-expression plasmid for production of lutein (and zeaxanthin) when coexpressed with a plasmid to biosynthesize α-carotene (and β-carotene) in E. coli.Takemura, M., Biosynthetic routes of hydroxylated carotenoids (xanthophylls) in Marchantia polymorpha, and production of novel and rare xanthophylls through pathway engineering in Escherichia coli. Planta 241 (3): 699-710 (2015). PMID 25467956. (original). [link to RRC of NBRP]
RDB19522CDF-MpCYP97C-MpLCYeMpCYP97C- and MpLCYe-expression plasmid for production of lutein when coexpreessd with a plasmid to biosynthsize zeinoxanthin in E. coli.Takemura, M., Pathway engineering for high-yield production of lutein in Escherichia coli. Synth. Biol. (Oxf) 6 (1): ysab012 (2021). PMID 34712837. (original). [link to RRC of NBRP]

go to top


2024.04.02

depxxxx_html_4_230809.pl