Resource data sheet
DNA Bank Top

pRK-idi (#RDB19490)

Expression plasmid of the isopentenyl diphoshate (IPP) isomerase gene (IDI), which is used to incerease carotenoid production with another carotenogenic plasmid in E. coli (XdIDI).


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Albrecht, M., Biotechnology Letters 21: 791–795 (1999). [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Expression plasmid of the isopentenyl diphoshate (IPP) isomerase gene (IDI), which is used to incerease carotenoid production with another carotenogenic plasmid in E. coli. See Table 1 of Albrecht, M. et al., Biotechnol. Lett., 21 (9): 791-795, 1999.
Vector backbone pRK404 (plasmid)
Selectable markers tetracycline
Growth conditions LB medium including 12.5 mg/L of Tc at 37℃
Gene/insert name Xanthophyllomyces dendrorhous XdIDI.
Reference of insert sequence LC645088 (DDBJ accession number)
Depositor|Developer Misawa, Norihiko |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (M, Albrecht. et al, Biotechnol. Lett., 21, 791-795, 1999)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (M, Albrecht. et al, Biotechnol. Lett., 21, 791-795, 1999)。

Catalog # Resource name Shipping form Fee
RDB19490 pRK-idi DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pRK-idi was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19490).

Reference section:

Albrecht, M., Misawa, N., Sandmann, G., Metabolic engineering of the terpenoid biosynthetic pathway of Escherichia coli for production of the carotenoids β-carotene and zeaxanthin. Biotechnology Letters 21: 791–795 (1999). [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Albrecht, M., Metabolic engineering of the terpenoid biosynthetic pathway of Escherichia coli for production of the carotenoids β-carotene and zeaxanthin. Biotechnology Letters 21: 791–795 (1999). [Article] [RRC of NBRP]
reference Matsumoto, W., Carotenoid productivity in human intestinal bacteria Eubacterium limosum and Leuconostoc mesenteroides with functional analysis of their carotenoid biosynthesis genes. Engineering Microbiology 4 (2): 100147 (2024). [Article]
reference Misawa, N., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [PubMed] [Article] [RRC of NBRP]